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Protein

2'-5'-oligoadenylate synthase 1A

Gene

Oas1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L.

Catalytic activityi

3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection. The dsRNA activator must be at least 15 nucleotides long, and no modification of the 2'-hydroxyl group is tolerated. ssRNA or dsDNA do not act as activators.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641ATPBy similarity
Metal bindingi76 – 761Magnesium; catalyticBy similarity
Metal bindingi78 – 781Magnesium; catalyticBy similarity
Metal bindingi149 – 1491Magnesium; catalyticBy similarity
Binding sitei214 – 2141ATPBy similarity
Binding sitei231 – 2311ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi2.7.7.84. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-oligoadenylate synthase 1A (EC:2.7.7.84)
Short name:
(2-5')oligo(A) synthase 1A
Short name:
2-5A synthase 1A
Gene namesi
Name:Oas1a
Synonyms:Oas1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621760. Oas1a.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3583582'-5'-oligoadenylate synthase 1APRO_0000160263Add
BLAST

Proteomic databases

PaxDbiQ05961.
PRIDEiQ05961.

PTM databases

iPTMnetiQ05961.
PhosphoSiteiQ05961.

Interactioni

Subunit structurei

Monomer. Homotetramer.By similarity

Protein-protein interaction databases

MINTiMINT-4563702.
STRINGi10116.ENSRNOP00000036734.

Structurei

3D structure databases

ProteinModelPortaliQ05961.
SMRiQ05961. Positions 4-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 6148Interaction with dsRNABy similarityAdd
BLAST
Regioni201 – 21111Interaction with dsRNABy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
HOGENOMiHOG000022614.
HOVERGENiHBG000994.
InParanoidiQ05961.
KOiK14216.

Family and domain databases

Gene3Di1.10.1410.20. 1 hit.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 1 hit.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 1 hit.
PS50152. 25A_SYNTH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQELRSTPS WKLDKFIEVY LLPNTSFRDD VKSAINVLCD FLKERCFRDT
60 70 80 90 100
VHPVRVSKVV KGGSSGKGTT LKGKSDADLV VFLNNFTSFE DQLNRRGEFI
110 120 130 140 150
KEIKKQLYEV QREKHFRVKF EVQSSWWPNP RALSFKLSAP HLQQEVEFDV
160 170 180 190 200
LPAYDVLGHV SLYSNPDPKI YTILISECIS LGKDGEFSTC FTELQRNFLK
210 220 230 240 250
QRPTKLKSLI RLVKHWYQLC KEKLGKPLPP QYALELLTVY AWERGNGITE
260 270 280 290 300
FNTAQGFRTI LELVTKYQQL RIYWTKYYDF QHPDVSKYLH RQLRKSRPVI
310 320 330 340 350
LDPADPTGNV AGGNQEGWRR LASEARLWLQ YPCFMNRGGS PVSSWEVPVD

EAWSCILL
Length:358
Mass (Da):41,666
Last modified:February 1, 1994 - v1
Checksum:iAE0F24E6A5D45BEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18877 mRNA. Translation: CAA79317.1.
PIRiS31407.
RefSeqiNP_620268.1. NM_138913.1.
UniGeneiRn.10383.

Genome annotation databases

GeneIDi192281.
KEGGirno:192281.
UCSCiRGD:621760. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18877 mRNA. Translation: CAA79317.1.
PIRiS31407.
RefSeqiNP_620268.1. NM_138913.1.
UniGeneiRn.10383.

3D structure databases

ProteinModelPortaliQ05961.
SMRiQ05961. Positions 4-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4563702.
STRINGi10116.ENSRNOP00000036734.

PTM databases

iPTMnetiQ05961.
PhosphoSiteiQ05961.

Proteomic databases

PaxDbiQ05961.
PRIDEiQ05961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192281.
KEGGirno:192281.
UCSCiRGD:621760. rat.

Organism-specific databases

CTDi246730.
RGDi621760. Oas1a.

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
HOGENOMiHOG000022614.
HOVERGENiHBG000994.
InParanoidiQ05961.
KOiK14216.

Enzyme and pathway databases

BRENDAi2.7.7.84. 5301.

Miscellaneous databases

NextBioi623006.
PROiQ05961.

Family and domain databases

Gene3Di1.10.1410.20. 1 hit.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 1 hit.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 1 hit.
PS50152. 25A_SYNTH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Transgenic potato plants expressing mammalian 2'-5' oligoadenylate synthetase are protected from potato virus X infection under field conditions."
    Truve E., Aaspollu A., Honkanen J., Puska R., Mehto M., Hassi A., Teeri T.H., Kelve M., Seppanen P., Saarma M.
    Biotechnology (N.Y.) 11:1048-1052(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The mammalian 2'-5' oligoadenylate synthetase gene family: evidence for concerted evolution of paralogous Oas1 genes in Rodentia and Artiodactyla."
    Perelygin A.A., Zharkikh A.A., Scherbik S.V., Brinton M.A.
    J. Mol. Evol. 63:562-576(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiOAS1A_RAT
AccessioniPrimary (citable) accession number: Q05961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 20, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.