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Protein

Sterol regulatory element-binding protein ECM22

Gene

ECM22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor involved in sterol uptake and regulation of sterol biosynthesis. Binds to sterol regulatory elements (SRE) with the consensus sequence 5'-TCGTATA-3' present in ERG2 and ERG3 promoters and regulates transcription of ERG2 and ERG3 in response to sterol levels. Seems to be involved in activation of DAN2 and DAN3 cell wall mannoproteins.3 Publications

Miscellaneous

Present with 79 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi43 – 73Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32342-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol regulatory element-binding protein ECM22
Alternative name(s):
Extracellular mutant protein 22
Gene namesi
Name:ECM22
Ordered Locus Names:YLR228C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR228C
SGDiS000004218 ECM22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

ECM22 and UPC2 (in combination) null mutants are not viable.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi790G → D: Increases aerobic sterol uptake. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149481 – 814Sterol regulatory element-binding protein ECM22Add BLAST814

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei431PhosphoserineCombined sources1
Modified residuei445PhosphoserineCombined sources1
Modified residuei464PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05958
PaxDbiQ05958
PRIDEiQ05958

PTM databases

iPTMnetiQ05958

Interactioni

Protein-protein interaction databases

BioGridi31498, 103 interactors
DIPiDIP-5951N
IntActiQ05958, 15 interactors
STRINGi4932.YLR228C

Structurei

3D structure databases

ProteinModelPortaliQ05958
SMRiQ05958
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00390000013273
HOGENOMiHOG000001044
InParanoidiQ05958
OMAiPTAWIFH
OrthoDBiEOG092C3B2O

Family and domain databases

CDDicd00067 GAL4, 1 hit
Gene3Di4.10.240.10, 1 hit
InterProiView protein in InterPro
IPR021858 Fun_TF
IPR001138 Zn2-C6_fun-type_DNA-bd
IPR036864 Zn2-C6_fun-type_DNA-bd_sf
PfamiView protein in Pfam
PF11951 Fungal_trans_2, 1 hit
PF00172 Zn_clus, 1 hit
SMARTiView protein in SMART
SM00066 GAL4, 1 hit
SUPFAMiSSF57701 SSF57701, 1 hit
PROSITEiView protein in PROSITE
PS00463 ZN2_CY6_FUNGAL_1, 1 hit
PS50048 ZN2_CY6_FUNGAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q05958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSDDGNAGQ EREKDAELIE VGGKKVSKTS TGKRKFHNKS KTGCDNCKRR
60 70 80 90 100
RVKCDEGKPF CKKCTNMKLD CVYSPIQPRR RKDSSSSKFA SAVHDRVGKK
110 120 130 140 150
NLSDNAIMLQ QQQQQLHHQQ EQQFRQQQQV QLQQQLLPHV GTDEQSNSPN
160 170 180 190 200
SVPPSVSNNM ENLLLPHLLA SLVNNTSNST NSSANGAEAH NNITQTAPSS
210 220 230 240 250
MINNNHPNMA LPGNSPLSIP ITPSFQSTAM NLSSSLNGLL SPGRLNSVTN
260 270 280 290 300
GLQQPQLQQQ NQQIPQQQGT QSPFSNIPFD QLAQLNKMGL NFNMKSFNTL
310 320 330 340 350
FPYGAANGMA SEFQELFGLG KFATSNNRAI KVSTAEEALA NMQQEQEDKN
360 370 380 390 400
KQFTKNPLDN TKTDAVNSGN NPLNGNENKV TASDILSHNK NLIIDNTGLT
410 420 430 440 450
ISPPHTLSKP SIDQNIASPS TGVSNVTSTK SLLSIPDNRT ALGNSPTLKT
460 470 480 490 500
SPMGDLLSNS EALSPRSSNS HTQQQSSPHS NASSASRLVP ELVGLSRKSN
510 520 530 540 550
LNLIDLKLFH HYCTDVWHTI TEAGISGPEV WSTYIPDLAF HFPFLMHTIL
560 570 580 590 600
AFSATHLSRT EAGLDNYVSS HRLEALRLLR EAVLEISDDN TDALVASALI
610 620 630 640 650
LILDSLANAS SSSPTAWIFH VKGAVTILTA VWPLSETSKF YNLISVDLSD
660 670 680 690 700
LGEAVINQSN HNNDNDNSNN GDGNNNNTIS ELVCFDESIA DLYPVEIDSP
710 720 730 740 750
YLITLAYLDK LHREKNQLDF MLRVFSFPAL LDRTFLALLM TGDLGAMRIM
760 770 780 790 800
RSYYTLLRGY TTEIKDKVWF LDSVSQVLPQ DVDEYSGGGG MHMMLDFLGG
810
GLPSMTTTNF SAFM
Length:814
Mass (Da):89,286
Last modified:November 1, 1996 - v1
Checksum:i48E3BDF32D7C668F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19027 Genomic DNA Translation: AAB67415.1
BK006945 Genomic DNA Translation: DAA09546.1
PIRiS51451
RefSeqiNP_013329.1, NM_001182115.1

Genome annotation databases

EnsemblFungiiYLR228C; YLR228C; YLR228C
GeneIDi850928
KEGGisce:YLR228C

Similar proteinsi

Entry informationi

Entry nameiECM22_YEAST
AccessioniPrimary (citable) accession number: Q05958
Secondary accession number(s): D6VYN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health