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Protein

Sterol regulatory element-binding protein ECM22

Gene

ECM22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in sterol uptake and regulation of sterol biosynthesis. Binds to sterol regulatory elements (SRE) with the consensus sequence 5'-TCGTATA-3' present in ERG2 and ERG3 promoters and regulates transcription of ERG2 and ERG3 in response to sterol levels. Seems to be involved in activation of DAN2 and DAN3 cell wall mannoproteins.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi43 – 7331Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32342-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol regulatory element-binding protein ECM22
Alternative name(s):
Extracellular mutant protein 22
Gene namesi
Name:ECM22
Ordered Locus Names:YLR228C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR228C.
SGDiS000004218. ECM22.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: SGD
  • nucleus Source: SGD
  • perinuclear region of cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

ECM22 and UPC2 (in combination) null mutants are not viable.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi790 – 7901G → D: Increases aerobic sterol uptake. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 814814Sterol regulatory element-binding protein ECM22PRO_0000114948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei431 – 4311PhosphoserineCombined sources
Modified residuei445 – 4451PhosphoserineCombined sources
Modified residuei464 – 4641PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05958.

PTM databases

iPTMnetiQ05958.

Interactioni

Protein-protein interaction databases

BioGridi31498. 50 interactions.
DIPiDIP-5951N.
IntActiQ05958. 5 interactions.
MINTiMINT-4495485.

Structurei

3D structure databases

ProteinModelPortaliQ05958.
SMRiQ05958. Positions 500-780.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000013273.
HOGENOMiHOG000001044.
InParanoidiQ05958.
OMAiPTAWIFH.
OrthoDBiEOG7327ZS.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR021858. Fun_TF.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF11951. Fungal_trans_2. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSDDGNAGQ EREKDAELIE VGGKKVSKTS TGKRKFHNKS KTGCDNCKRR
60 70 80 90 100
RVKCDEGKPF CKKCTNMKLD CVYSPIQPRR RKDSSSSKFA SAVHDRVGKK
110 120 130 140 150
NLSDNAIMLQ QQQQQLHHQQ EQQFRQQQQV QLQQQLLPHV GTDEQSNSPN
160 170 180 190 200
SVPPSVSNNM ENLLLPHLLA SLVNNTSNST NSSANGAEAH NNITQTAPSS
210 220 230 240 250
MINNNHPNMA LPGNSPLSIP ITPSFQSTAM NLSSSLNGLL SPGRLNSVTN
260 270 280 290 300
GLQQPQLQQQ NQQIPQQQGT QSPFSNIPFD QLAQLNKMGL NFNMKSFNTL
310 320 330 340 350
FPYGAANGMA SEFQELFGLG KFATSNNRAI KVSTAEEALA NMQQEQEDKN
360 370 380 390 400
KQFTKNPLDN TKTDAVNSGN NPLNGNENKV TASDILSHNK NLIIDNTGLT
410 420 430 440 450
ISPPHTLSKP SIDQNIASPS TGVSNVTSTK SLLSIPDNRT ALGNSPTLKT
460 470 480 490 500
SPMGDLLSNS EALSPRSSNS HTQQQSSPHS NASSASRLVP ELVGLSRKSN
510 520 530 540 550
LNLIDLKLFH HYCTDVWHTI TEAGISGPEV WSTYIPDLAF HFPFLMHTIL
560 570 580 590 600
AFSATHLSRT EAGLDNYVSS HRLEALRLLR EAVLEISDDN TDALVASALI
610 620 630 640 650
LILDSLANAS SSSPTAWIFH VKGAVTILTA VWPLSETSKF YNLISVDLSD
660 670 680 690 700
LGEAVINQSN HNNDNDNSNN GDGNNNNTIS ELVCFDESIA DLYPVEIDSP
710 720 730 740 750
YLITLAYLDK LHREKNQLDF MLRVFSFPAL LDRTFLALLM TGDLGAMRIM
760 770 780 790 800
RSYYTLLRGY TTEIKDKVWF LDSVSQVLPQ DVDEYSGGGG MHMMLDFLGG
810
GLPSMTTTNF SAFM
Length:814
Mass (Da):89,286
Last modified:November 1, 1996 - v1
Checksum:i48E3BDF32D7C668F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19027 Genomic DNA. Translation: AAB67415.1.
BK006945 Genomic DNA. Translation: DAA09546.1.
PIRiS51451.
RefSeqiNP_013329.1. NM_001182115.1.

Genome annotation databases

EnsemblFungiiYLR228C; YLR228C; YLR228C.
GeneIDi850928.
KEGGisce:YLR228C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19027 Genomic DNA. Translation: AAB67415.1.
BK006945 Genomic DNA. Translation: DAA09546.1.
PIRiS51451.
RefSeqiNP_013329.1. NM_001182115.1.

3D structure databases

ProteinModelPortaliQ05958.
SMRiQ05958. Positions 500-780.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31498. 50 interactions.
DIPiDIP-5951N.
IntActiQ05958. 5 interactions.
MINTiMINT-4495485.

PTM databases

iPTMnetiQ05958.

Proteomic databases

MaxQBiQ05958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR228C; YLR228C; YLR228C.
GeneIDi850928.
KEGGisce:YLR228C.

Organism-specific databases

EuPathDBiFungiDB:YLR228C.
SGDiS000004218. ECM22.

Phylogenomic databases

GeneTreeiENSGT00390000013273.
HOGENOMiHOG000001044.
InParanoidiQ05958.
OMAiPTAWIFH.
OrthoDBiEOG7327ZS.

Enzyme and pathway databases

BioCyciYEAST:G3O-32342-MONOMER.

Miscellaneous databases

PROiQ05958.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR021858. Fun_TF.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF11951. Fungal_trans_2. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake."
    Shianna K.V., Dotson W.D., Tove S., Parks L.W.
    J. Bacteriol. 183:830-834(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-790, DISRUPTION PHENOTYPE.
  4. "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae."
    Abramova N.E., Sertil O., Mehta S., Lowry C.V.
    J. Bacteriol. 183:2881-2887(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Upc2p and Ecm22p, dual regulators of sterol biosynthesis in Saccharomyces cerevisiae."
    Vik A., Rine J.
    Mol. Cell. Biol. 21:6395-6405(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431; SER-445 AND SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiECM22_YEAST
AccessioniPrimary (citable) accession number: Q05958
Secondary accession number(s): D6VYN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 79 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.