ID   BUR2_YEAST              Reviewed;         395 AA.
AC   Q05949;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Protein BUR2;
DE   AltName: Full=Bypass UAS requirement protein 2;
DE   AltName: Full=Chromosome stability protein 4;
GN   Name=BUR2; Synonyms=CST4; OrderedLocusNames=YLR226W;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313267; PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
RA   Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
RA   Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
RA   Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
RA   Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
RA   Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
RA   Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
RA   Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
RA   Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
RA   Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
RA   Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
RA   Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   FUNCTION.
RX   PubMed=8293972;
RA   Prelich G., Winston F.;
RT   "Mutations that suppress the deletion of an upstream activating
RT   sequence in yeast: involvement of a protein kinase and histone H3 in
RT   repressing transcription in vivo.";
RL   Genetics 135:665-676(1993).
RN   [3]
RP   GENE NAME.
RX   MEDLINE=99384339; PubMed=10454593; DOI=10.1093/nar/27.15.3001;
RA   Ouspenski I.I., Elledge S.J., Brinkley B.R.;
RT   "New yeast genes important for chromosome integrity and segregation
RT   identified by dosage effects on genome stability.";
RL   Nucleic Acids Res. 27:3001-3008(1999).
RN   [4]
RP   INTERACTION WITH BUR1, AND FUNCTION.
RX   PubMed=10982824; DOI=10.1128/MCB.20.19.7080-7087.2000;
RA   Yao S., Neiman A., Prelich G.;
RT   "BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin
RT   complex important for transcription in vivo.";
RL   Mol. Cell. Biol. 20:7080-7087(2000).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   FUNCTION.
RX   PubMed=16040246; DOI=10.1016/j.cub.2005.07.028;
RA   Laribee R.N., Krogan N.J., Xiao T., Shibata Y., Hughes T.R.,
RA   Greenblatt J.F., Strahl B.D.;
RT   "BUR kinase selectively regulates H3 K4 trimethylation and H2B
RT   ubiquitylation through recruitment of the PAF elongation complex.";
RL   Curr. Biol. 15:1487-1493(2005).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, AND MASS
RP   SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Component of the BUR kinase complex involved in
CC       transcription regulation. This complex phosphorylates 'Ser-120' of
CC       the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to
CC       monoubiquitination of histone H2B, the localization of the PAF1
CC       complex to the chromatin, and the silencing of telomeric-
CC       associated genes. Also required for histone H3 'Lys-4'
CC       trimethylation. May phosphorylate the 'Ser-5' of the RBP1 carboxy-
CC       terminal domain (CTD) repeats. Necessary for the recovery from
CC       pheromone-induced growth arrest in the cell cycle G1 phase. Also
CC       required for vegetative growth itself. The kinase activity of the
CC       complex requires the presence of BUR2. Overexpression of BUR2
CC       interferes with mitotic chromosome segregation.
CC   -!- SUBUNIT: Belongs to the BUR kinase complex composed of SGV1/BUR1
CC       and BUR2. Interacts with SGV1.
CC   -!- INTERACTION:
CC       P23293:SGV1; NbExp=2; IntAct=EBI-30948, EBI-17078;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Present with 2910 molecules/cell in log phase SD
CC       medium.
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DR   EMBL; U19027; AAB67407.1; -; Genomic_DNA.
DR   PIR; S51449; S51449.
DR   RefSeq; NP_013327.1; -.
DR   DIP; DIP:4787N; -.
DR   IntAct; Q05949; 7.
DR   Ensembl; YLR226W; Saccharomyces cerevisiae.
DR   GeneID; 850923; -.
DR   GenomeReviews; Y13138_GR; YLR226W.
DR   KEGG; sce:YLR226W; -.
DR   NMPDR; fig|4932.3.peg.4338; -.
DR   CYGD; YLR226w; -.
DR   SGD; S000004216; BUR2.
DR   HOGENOM; Q05949; -.
DR   OMA; Q05949; MEKCLMY.
DR   NextBio; 967350; -.
DR   ArrayExpress; Q05949; -.
DR   GermOnline; YLR226W; Saccharomyces cerevisiae.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0016538; F:cyclin-dependent protein kinase regulator a...; IDA:SGD.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IGI:SGD.
DR   GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0006974; P:response to DNA damage stimulus; IMP:SGD.
DR   GO; GO:0006350; P:transcription; IDA:SGD.
PE   1: Evidence at protein level;
KW   Cell cycle; Complete proteome; Nucleus; Phosphoprotein; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    395       Protein BUR2.
FT                                /FTId=PRO_0000076186.
FT   MOD_RES      24     24       Phosphoserine.
SQ   SEQUENCE   395 AA;  45721 MW;  B317A7B429030FDF CRC64;
     MSATSSSGDV KKFQAVPKPT SNASPPPASS GFNARTLWPD LIETPENQWV FECKDIIEKI
     GTNGPIAVEI KKNMEKCLMY FYTLKKKLNL FDHTYTASCI LFYRYWFIYG IPTAITECIH
     ISQGILVTAC KTMENNRPIE AYIKATCEFL MQNIPSLKSR TNIDKLKWEF RDKLVTNEKK
     ILCLFGFDLN ISNPKELIEE VFSGYYRFNR DHNLPENFKK AFPKILQESR NFMVQAVTQP
     VSLLCDGYTF IVLSLIYCGL EYKKLVDKDF RYPKNFFKDR FPIEVTPENF ANIFTDYKLL
     EENFFNLKSN KGAKLQIDSS MIDSVIDESG DVENEVSEIS DPFNYELIKS GEVKEEFLNH
     IETRVKDLLD KAKQESMKRK AKDPIRTPDA KKPKI
//