Reviewed, UniProtKB/Swiss-Prot Q05949 (BUR2_YEAST)

Names and origin

Protein names

Recommended name:
    Protein BUR2
Alternative name(s):
    Bypass UAS requirement protein 2
    Chromosome stability protein 4

Gene names

Name:	BUR2
Synonyms:	CST4
Ordered Locus Names:	YLR226W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the BUR kinase complex involved in transcription regulation. This complex phosphorylates 'Ser-120' of the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B, the localization of the PAF1 complex to the chromatin, and the silencing of telomeric-associated genes. Also required for histone H3 'Lys-4' trimethylation. May phosphorylate the 'Ser-5' of the RBP1 carboxy-terminal domain (CTD) repeats. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase. Also required for vegetative growth itself. The kinase activity of the complex requires the presence of BUR2. Overexpression of BUR2 interferes with mitotic chromosome segregation. Ref.2 Ref.4 Ref.7
Subunit structure	Belongs to the BUR kinase complex composed of SGV1/BUR1 and BUR2. Interacts with SGV1. Ref.4
Subcellular location	Nucleus. Ref.5
Miscellaneous	Present with 2910 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitotic sister chromatid segregation Ref.3 Inferred from genetic interaction. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulus Inferred from mutant phenotype. Source: SGD transcription Ref.4 Inferred from direct assay. Source: SGD
Cellular component	nucleus Ref.5 Inferred from direct assay. Source: SGD
Molecular function	cyclin-dependent protein kinase regulator activity Ref.4 Inferred from direct assay. Source: SGD protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SGV1	P23293	2	EBI-30948,EBI-17078

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Protein BUR2		PRO_0000076186
Amino acid modifications
	Modified residue	24	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q05949-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B317A7B429030FDF	FASTA	395	45,721
	10 20 30 40 50 60 MSATSSSGDV KKFQAVPKPT SNASPPPASS GFNARTLWPD LIETPENQWV FECKDIIEKI 70 80 90 100 110 120 GTNGPIAVEI KKNMEKCLMY FYTLKKKLNL FDHTYTASCI LFYRYWFIYG IPTAITECIH 130 140 150 160 170 180 ISQGILVTAC KTMENNRPIE AYIKATCEFL MQNIPSLKSR TNIDKLKWEF RDKLVTNEKK 190 200 210 220 230 240 ILCLFGFDLN ISNPKELIEE VFSGYYRFNR DHNLPENFKK AFPKILQESR NFMVQAVTQP 250 260 270 280 290 300 VSLLCDGYTF IVLSLIYCGL EYKKLVDKDF RYPKNFFKDR FPIEVTPENF ANIFTDYKLL 310 320 330 340 350 360 EENFFNLKSN KGAKLQIDSS MIDSVIDESG DVENEVSEIS DPFNYELIKS GEVKEEFLNH 370 380 390 IETRVKDLLD KAKQESMKRK AKDPIRTPDA KKPKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo." Prelich G., Winston F. Genetics 135:665-676(1993) [PubMed: 8293972] [Abstract] Cited for: FUNCTION.
[3]	"New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability." Ouspenski I.I., Elledge S.J., Brinkley B.R. Nucleic Acids Res. 27:3001-3008(1999) [PubMed: 10454593] [Abstract] Cited for: GENE NAME.
[4]	"BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo." Yao S., Neiman A., Prelich G. Mol. Cell. Biol. 20:7080-7087(2000) [PubMed: 10982824] [Abstract] Cited for: INTERACTION WITH BUR1, FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex." Laribee R.N., Krogan N.J., Xiao T., Shibata Y., Hughes T.R., Greenblatt J.F., Strahl B.D. Curr. Biol. 15:1487-1493(2005) [PubMed: 16040246] [Abstract] Cited for: FUNCTION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19027 Genomic DNA. Translation: AAB67407.1.
PIR	S51449.
RefSeq	NP_013327.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4787N.
IntAct	Q05949. 7 interactions.
Genome annotation databases
Ensembl	YLR226W. Saccharomyces cerevisiae. [Contig view]
GeneID	850923.
GenomeReviews	Gene locus YLR226W in contig Y13138_GR.
KEGG	sce:YLR226W.
NMPDR	fig|4932.3.peg.4338.
Organism-specific databases
CYGD	YLR226w.
SGD	S000004216. BUR2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q05949.
OMA	Q05949. MEKCLMY.
Gene expression databases
ArrayExpress	Q05949.
GermOnline	YLR226W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	967350.

Entry information

Entry name

BUR2_YEAST

Accession

Primary (citable) accession number: Q05949

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)