Protein BUR2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q05949 (BUR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein BUR2

Alternative name(s):
Bypass UAS requirement protein 2
Chromosome stability protein 4

Gene names

Name:	BUR2
Synonyms:	CST4
Ordered Locus Names:	YLR226W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the BUR kinase complex involved in transcription regulation. This complex phosphorylates 'Ser-120' of the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B, the localization of the PAF1 complex to the chromatin, and the silencing of telomeric-associated genes. Also required for histone H3 'Lys-4' trimethylation. May phosphorylate the 'Ser-5' of the RBP1 carboxy-terminal domain (CTD) repeats. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase. Also required for vegetative growth itself. The kinase activity of the complex requires the presence of BUR2. Overexpression of BUR2 interferes with mitotic chromosome segregation. Ref.3 Ref.5 Ref.8
Subunit structure	Belongs to the BUR kinase complex composed of SGV1/BUR1 and BUR2. Interacts with SGV1. Ref.5
Subcellular location	Nucleus Ref.6.
Miscellaneous	Present with 2910 molecules/cell in log phase SD medium. Ref.7

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitotic sister chromatid segregation Inferred from genetic interaction Ref.4. Source: SGD phosphorylation of RNA polymerase II C-terminal domain Inferred from mutant phenotype. Source: SGD positive regulation of histone H3-K4 methylation Inferred from mutant phenotype Ref.8. Source: SGD positive regulation of histone ubiquitination Inferred from mutant phenotype Ref.8. Source: SGD response to DNA damage stimulus Inferred from mutant phenotype. Source: SGD transcription, DNA-dependent Inferred from physical interaction Ref.5. Source: SGD
Cellular component	cyclin-dependent protein kinase holoenzyme complex Inferred from physical interaction Ref.5. Source: SGD nucleus Inferred from direct assay Ref.6. Source: SGD
Molecular function	cyclin-dependent protein kinase regulator activity Inferred from physical interaction Ref.5. Source: SGD protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SGV1	P23293	6	EBI-30948,EBI-17078

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 395

395

Protein BUR2

PRO_0000076186

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

Q05949 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B317A7B429030FDF
Show »

FASTA

395

45,721

        10         20         30         40         50         60 
MSATSSSGDV KKFQAVPKPT SNASPPPASS GFNARTLWPD LIETPENQWV FECKDIIEKI 

        70         80         90        100        110        120 
GTNGPIAVEI KKNMEKCLMY FYTLKKKLNL FDHTYTASCI LFYRYWFIYG IPTAITECIH 

       130        140        150        160        170        180 
ISQGILVTAC KTMENNRPIE AYIKATCEFL MQNIPSLKSR TNIDKLKWEF RDKLVTNEKK 

       190        200        210        220        230        240 
ILCLFGFDLN ISNPKELIEE VFSGYYRFNR DHNLPENFKK AFPKILQESR NFMVQAVTQP 

       250        260        270        280        290        300 
VSLLCDGYTF IVLSLIYCGL EYKKLVDKDF RYPKNFFKDR FPIEVTPENF ANIFTDYKLL 

       310        320        330        340        350        360 
EENFFNLKSN KGAKLQIDSS MIDSVIDESG DVENEVSEIS DPFNYELIKS GEVKEEFLNH 

       370        380        390 
IETRVKDLLD KAKQESMKRK AKDPIRTPDA KKPKI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo." Prelich G., Winston F. Genetics 135:665-676(1993) [PubMed: 8293972] [Abstract] Cited for: FUNCTION.
[4]	"New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability." Ouspenski I.I., Elledge S.J., Brinkley B.R. Nucleic Acids Res. 27:3001-3008(1999) [PubMed: 10454593] [Abstract] Cited for: GENE NAME.
[5]	"BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo." Yao S., Neiman A., Prelich G. Mol. Cell. Biol. 20:7080-7087(2000) [PubMed: 10982824] [Abstract] Cited for: INTERACTION WITH BUR1, FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex." Laribee R.N., Krogan N.J., Xiao T., Shibata Y., Hughes T.R., Greenblatt J.F., Strahl B.D. Curr. Biol. 15:1487-1493(2005) [PubMed: 16040246] [Abstract] Cited for: FUNCTION.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19027 Genomic DNA. Translation: AAB67407.1. BK006945 Genomic DNA. Translation: DAA09542.1.
PIR	S51449.
RefSeq	NP_013327.1. NM_001182113.1.
3D structure databases
ProteinModelPortal	Q05949.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4787N.
IntAct	Q05949. 4 interactions.
MINT	MINT-507428.
STRING	Q05949.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR226W; YLR226W; YLR226W.
GeneID	850923.
KEGG	sce:YLR226W.
NMPDR	fig\|4932.3.peg.4338.
Organism-specific databases
CYGD	YLR226w.
SGD	S000004216. BUR2.
Phylogenomic databases
eggNOG	fuNOG08201.
HOGENOM	HBG202504.
OMA	MEKCLMY.
OrthoDB	EOG4BS0VM.
Gene expression databases
ArrayExpress	Q05949.
Genevestigator	Q05949.
GermOnline	YLR226W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013763. Cyclin-like. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 1 hit.
KO	K15563.
SUPFAM	SSF47954. Cyclin_like. 1 hit.
ProtoNet	Search...
Other
NextBio	967350.

Entry information

Entry name

BUR2_YEAST

Accession

Primary (citable) accession number: Q05949
Secondary accession number(s): D6VYM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents