Reviewed,
UniProtKB/Swiss-Prot Q05949 (BUR2_YEAST)
Last modified
June 16, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
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Names and origin
| Protein names | Recommended name: Protein BUR2 Alternative name(s): Bypass UAS requirement protein 2 Chromosome stability protein 4 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 395 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the BUR kinase complex involved in transcription regulation. This complex phosphorylates 'Ser-120' of the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B, the localization of the PAF1 complex to the chromatin, and the silencing of telomeric-associated genes. Also required for histone H3 'Lys-4' trimethylation. May phosphorylate the 'Ser-5' of the RBP1 carboxy-terminal domain (CTD) repeats. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase. Also required for vegetative growth itself. The kinase activity of the complex requires the presence of BUR2. Overexpression of BUR2 interferes with mitotic chromosome segregation. Ref.2 Ref.4 Ref.7 |
| Subunit structure | Belongs to the BUR kinase complex composed of SGV1/BUR1 and BUR2. Interacts with SGV1. Ref.4 |
| Subcellular location | |
| Miscellaneous | Present with 2910 molecules/cell in log phase SD medium. Ref.6 |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell cycle Transcription Transcription regulation |
| Cellular component | Nucleus |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | mitotic sister chromatid segregation Ref.3 Inferred from genetic interaction. Source: SGD regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulusInferred from mutant phenotype. Source: SGD transcription Ref.4Inferred from direct assay. Source: SGD |
| Cellular component | nucleus Ref.5 Inferred from direct assay. Source: SGD |
| Molecular function | cyclin-dependent protein kinase regulator activity Ref.4 Inferred from direct assay. Source: SGD protein binding Ref.4Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D.Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | "Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo." Prelich G., Winston F. Genetics 135:665-676(1993) [PubMed: 8293972] [Abstract] Cited for: FUNCTION. |
| [3] | "New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability." Ouspenski I.I., Elledge S.J., Brinkley B.R. Nucleic Acids Res. 27:3001-3008(1999) [PubMed: 10454593] [Abstract] Cited for: GENE NAME. |
| [4] | "BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo." Yao S., Neiman A., Prelich G. Mol. Cell. Biol. 20:7080-7087(2000) [PubMed: 10982824] [Abstract] Cited for: INTERACTION WITH BUR1, FUNCTION. |
| [5] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex." Laribee R.N., Krogan N.J., Xiao T., Shibata Y., Hughes T.R., Greenblatt J.F., Strahl B.D. Curr. Biol. 15:1487-1493(2005) [PubMed: 16040246] [Abstract] Cited for: FUNCTION. |
| [8] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U19027 Genomic DNA. Translation: AAB67407.1. | |
| PIR | S51449. |
| RefSeq | NP_013327.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:4787N. |
| IntAct | Q05949. 7 interactions. |
Genome annotation databases | |
| Ensembl | YLR226W. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 850923. |
| GenomeReviews | Gene locus YLR226W in contig Y13138_GR. |
| KEGG | sce:YLR226W. |
| NMPDR | fig|4932.3.peg.4338. |
Organism-specific databases | |
| CYGD | YLR226w. |
| SGD | S000004216. BUR2. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | Q05949. |
| OMA | Q05949. MEKCLMY. |
Gene expression databases | |
| ArrayExpress | Q05949. |
| GermOnline | YLR226W. Saccharomyces cerevisiae. |
Family and domain databases | |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 967350. |
Entry information
| Entry name | BUR2_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q05949 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||

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