Q05946 (UTP13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U3 small nucleolar RNA-associated protein 13
Short name=U3 snoRNA-associated protein 13

Alternative name(s):
U three protein 13

Gene names

Name:	UTP13
Ordered Locus Names:	YLR222C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	817 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Ref.3
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.3
Subcellular location	Nucleus › nucleolus Ref.3.
Miscellaneous	Present with 8070 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 12 WD repeats.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat WD repeat
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD
Cellular component	Pwp2p-containing subcomplex of 90S preribosome Inferred from direct assay. Source: SGD small-subunit processome Inferred from direct assay Ref.3. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct snoRNA binding Inferred from physical interaction Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PWP2	P25635	7	EBI-34702,EBI-14332

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 817	817	U3 small nucleolar RNA-associated protein 13		PRO_0000051320
Regions
	Repeat	59 – 100	42	WD 1
	Repeat	102 – 139	38	WD 2
	Repeat	142 – 187	46	WD 3
	Repeat	191 – 233	43	WD 4
	Repeat	238 – 280	43	WD 5
	Repeat	386 – 425	40	WD 6
	Repeat	432 – 476	45	WD 7
	Repeat	489 – 528	40	WD 8
	Repeat	531 – 572	42	WD 9
	Repeat	573 – 614	42	WD 10
	Repeat	616 – 654	39	WD 11
	Repeat	664 – 705	42	WD 12
Amino acid modifications
	Modified residue	359	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q05946 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A3DEA4288917027D	FASTA	817	91,032
	10 20 30 40 50 60 MDLKTSYKGI SLNPIYAGSS AVATVSENGK ILATPVLDEI NIIDLTPGSR KILHKISNED 70 80 90 100 110 120 EQEITALKLT PDGQYLTYVS QAQLLKIFHL KTGKVVRSMK ISSPSYILDA DSTSTLLAVG 130 140 150 160 170 180 GTDGSIIVVD IENGYITHSF KGHGGTISSL KFYGQLNSKI WLLASGDTNG MVKVWDLVKR 190 200 210 220 230 240 KCLHTLQEHT SAVRGLDIIE VPDNDEPSLN LLSGGRDDII NLWDFNMKKK CKLLKTLPVN 250 260 270 280 290 300 QQVESCGFLK DGDGKRIIYT AGGDAIFQLI DSESGSVLKR TNKPIEELFI IGVLPILSNS 310 320 330 340 350 360 QMFLVLSDQT LQLINVEEDL KNDEDTIQVT SSIAGNHGII ADMRYVGPEL NKLALATNSP 370 380 390 400 410 420 SLRIIPVPDL SGPEASLPLD VEIYEGHEDL LNSLDATEDG LWIATASKDN TAIVWRYNEN 430 440 450 460 470 480 SCKFDIYAKY IGHSAAVTAV GLPNIVSKGY PEFLLTASND LTIKKWIIPK PTASMDVQII 490 500 510 520 530 540 KVSEYTRHAH EKDINALSVS PNDSIFATAS YDKTCKIWNL ENGELEATLA NHKRGLWDVS 550 560 570 580 590 600 FCQYDKLLAT SSGDKTVKIW SLDTFSVMKT LEGHTNAVQR CSFINKQKQL ISCGADGLIK 610 620 630 640 650 660 IWDCSSGECL KTLDGHNNRL WALSTMNDGD MIVSADADGV FQFWKDCTEQ EIEEEQEKAK 670 680 690 700 710 720 LQVEQEQSLQ NYMSKGDWTN AFLLAMTLDH PMRLFNVLKR ALGESRSRQD TEEGKIEVIF 730 740 750 760 770 780 NEELDQAISI LNDEQLILLM KRCRDWNTNA KTHTIAQRTI RCILMHHNIA KLSEIPGMVK 790 800 810 IVDAIIPYTQ RHFTRVDNLV EQSYILDYAL VEMDKLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed: 12068309] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19027 Genomic DNA. Translation: AAB67411.1. BK006945 Genomic DNA. Translation: DAA09538.1.
PIR	S51445.
RefSeq	NP_013323.1. NM_001182109.1.
3D structure databases
ProteinModelPortal	Q05946.
SMR	Q05946. Positions 17-272, 340-645.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4790N.
IntAct	Q05946. 29 interactions.
MINT	MINT-501291.
STRING	Q05946.
Proteomic databases
PeptideAtlas	Q05946.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR222C; YLR222C; YLR222C.
GeneID	850919.
KEGG	sce:YLR222C.
NMPDR	fig|4932.3.peg.4333.
Organism-specific databases
CYGD	YLR222c.
SGD	S000004212. UTP13.
Phylogenomic databases
eggNOG	fuNOG07044.
GeneTree	EFGT00050000000047.
HOGENOM	HBG397335.
OMA	ADSKLIR.
OrthoDB	EOG4DZ53K.
Gene expression databases
ArrayExpress	Q05946.
Genevestigator	Q05946.
GermOnline	YLR222C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR013934. SSU_processome_Utp13. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
KO	K14555.
Pfam	PF08625. Utp13. 1 hit. PF00400. WD40. 9 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 11 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 2 hits.
PROSITE	PS00678. WD_REPEATS_1. 3 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967338.

Entry information

Entry name

UTP13_YEAST

Accession

Primary (citable) accession number: Q05946
Secondary accession number(s): D6VYM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program