Q05941 (ACHA7_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-7

Gene names

Name:	Chrna7
Synonyms:	Acra7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Subunit structure	Homopentamer. Interacts with RIC3; which is required for proper folding and assembly. Ref.5 Ref.6
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	calcium ion transport Inferred from mutant phenotype. Source: MGI dopamine biosynthetic process Inferred from expression pattern. Source: RGD regulation of action potential in neuron Inferred from direct assay. Source: RGD synaptic transmission, cholinergic Inferred from mutant phenotype. Source: RGD
Cellular component	acetylcholine-gated channel complex Inferred from direct assay. Source: RGD asymmetric synapse Inferred from direct assay. Source: RGD axon Inferred from direct assay. Source: RGD cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay. Source: RGD dendritic shaft Inferred from direct assay. Source: RGD growth cone Inferred from direct assay. Source: RGD membrane raft Inferred from direct assay. Source: RGD neuronal cell body Inferred from direct assay. Source: RGD postsynaptic density Inferred from direct assay. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell presynaptic membrane Inferred from direct assay. Source: RGD
Molecular function	acetylcholine binding Inferred from direct assay. Source: RGD acetylcholine-activated cation-selective channel activity Inferred from direct assay. Source: MGI adenylate cyclase binding Inferred from physical interaction. Source: RGD drug binding Inferred from direct assay. Source: RGD protein kinase binding Inferred from physical interaction. Source: RGD receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APP	P05067	1	EBI-79422,EBI-77613	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 502	480	Neuronal acetylcholine receptor subunit alpha-7		PRO_0000000369
Regions
	Topological domain	23 – 230	208	Extracellular Potential
	Transmembrane	231 – 255	25	Helical; Potential
	Transmembrane	262 – 280	19	Helical; Potential
	Transmembrane	296 – 317	22	Helical; Potential
	Topological domain	318 – 469	152	Cytoplasmic Potential
	Transmembrane	470 – 490	21	Helical; Potential
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Disulfide bond	150 ↔ 164		By similarity
	Disulfide bond	212 ↔ 213		Associated with receptor activation By similarity
Experimental info
	Mutagenesis	433	1	L → A: Abolishes dependency on RIC3 for functional expression. Ref.5
	Mutagenesis	435	1	K → A: No effect on dependency on RIC3 for functional expression. Ref.5
	Mutagenesis	440	1	V → A: Impairs dependency on RIC3 for functional expression. Ref.5
	Mutagenesis	445	1	N → A: Slightly impairs dependency on RIC3 for functional expression. Ref.5
	Mutagenesis	446	1	R → A: Impairs dependency on RIC3 for functional expression. Ref.5
	Mutagenesis	447	1	F → A: Impairs dependency on RIC3 for functional expression. Ref.5
	Mutagenesis	448	1	R → A: Impairs dependency on RIC3 for functional expression. Ref.5
	Sequence conflict	447	1	F → N in AAB25224. Ref.1
	Sequence conflict	469	1	R → P in AAB25224. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05941 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 289A30498C7B9A58	FASTA	502	56,503
	10 20 30 40 50 60 MCGGRGGIWL ALAAALLHVS LQGEFQRRLY KELVKNYNPL ERPVANDSQP LTVYFSLSLL 70 80 90 100 110 120 QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN MSEYPGVKNV RFPDGQIWKP DILLYNSADE 130 140 150 160 170 180 RFDATFHTNV LVNASGHCQY LPPGIFKSSC YIDVRWFPFD VQQCKLKFGS WSYGGWSLDL 190 200 210 220 230 240 QMQEADISSY IPNGEWDLMG IPGKRNEKFY ECCKEPYPDV TYTVTMRRRT LYYGLNLLIP 250 260 270 280 290 300 CVLISALALL VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST 310 320 330 340 350 360 MIIVGLSVVV TVIVLRYHHH DPDGGKMPKW TRIILLNWCA WFLRMKRPGE DKVRPACQHK 370 380 390 400 410 420 PRRCSLASVE LSAGAGPPTS NGNLLYIGFR GLEGMHCAPT PDSGVVCGRL ACSPTHDEHL 430 440 450 460 470 480 MHGAHPSDGD PDLAKILEEV RYIANRFRCQ DESEVICSEW KFAACVVDRL CLMAFSVFTI 490 500 ICTIGILMSA PNFVEAVSKD FA

References

[1]	"Molecular cloning, functional properties, and distribution of rat brain alpha 7: a nicotinic cation channel highly permeable to calcium." Seguela P., Wadiche J., Dineley-Miller K., Dani J.A., Patrick J.W. J. Neurosci. 13:596-604(1993) [PubMed: 7678857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	Boulter J. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[3]	Hartley M. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 363. Strain: Sprague-Dawley. Tissue: Brain.
[4]	Groot-Kormelink P.J. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[5]	"Dual role of the RIC-3 protein in trafficking of serotonin and nicotinic acetylcholine receptors." Castillo M., Mulet J., Gutierrez L.M., Ortiz J.A., Castelan F., Gerber S., Sala S., Sala F., Criado M. J. Biol. Chem. 280:27062-27068(2005) [PubMed: 15927954] [Abstract] Cited for: INTERACTION WITH RIC3, MUTAGENESIS OF LEU-433; LYS-435; VAL-440; ASN-445; ARG-446; PHE-447 AND ARG-448.
[6]	"RIC-3 enhances functional expression of multiple nicotinic acetylcholine receptor subtypes in mammalian cells." Lansdell S.J., Gee V.J., Harkness P.C., Doward A.I., Baker E.R., Gibb A.J., Millar N.S. Mol. Pharmacol. 68:1431-1438(2005) [PubMed: 16120769] [Abstract] Cited for: INTERACTION WITH RIC3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S53987 mRNA. Translation: AAB25224.2. L31619 mRNA. Translation: AAC33136.1. AY574256 mRNA. Translation: AAS90352.1.
IPI	IPI00607154.
PIR	T01378.
RefSeq	NP_036964.3. NM_012832.3.
UniGene	Rn.9698.
3D structure databases
ProteinModelPortal	Q05941.
SMR	Q05941. Positions 227-261.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q05941. 1 interaction.
STRING	Q05941.
PTM databases
PhosphoSite	Q05941.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25302.
KEGG	rno:25302.
UCSC	AY671974. rat.
Organism-specific databases
CTD	1139.
RGD	2348. Chrna7.
Phylogenomic databases
eggNOG	roNOG14608.
HOVERGEN	HBG003756.
Gene expression databases
ArrayExpress	Q05941.
Genevestigator	Q05941.
GermOnline	ENSRNOG00000010853. Rattus norvegicus.
Family and domain databases
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KO	K04809.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606083.

Entry information

Entry name

ACHA7_RAT

Accession

Primary (citable) accession number: Q05941
Secondary accession number(s): Q53YK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program