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Q05940 (VMAT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name=VAT2
Gene names
Name:SLC18A2
Synonyms:SVMT, VMAT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

Subunit structure

Interacts with SLC6A3 By similarity.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

Sequence caution

The sequence BAB19009.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentCytoplasmic vesicle
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

cellular response to ammonium ion

Inferred from electronic annotation. Source: Ensembl

cellular response to drug

Inferred from electronic annotation. Source: Ensembl

death

Inferred from electronic annotation. Source: Ensembl

endocytic recycling

Inferred from electronic annotation. Source: Ensembl

glucose homeostasis

Inferred from electronic annotation. Source: Ensembl

insulin secretion

Inferred from electronic annotation. Source: Ensembl

locomotory behavior

Inferred from electronic annotation. Source: Ensembl

monoamine transport

Traceable author statement Ref.1. Source: ProtInc

negative regulation of neurotransmitter transport

Inferred from electronic annotation. Source: Ensembl

neurotransmitter secretion

Traceable author statement. Source: Reactome

post-embryonic development

Inferred from electronic annotation. Source: Ensembl

response to amphetamine

Inferred from electronic annotation. Source: Ensembl

response to cocaine

Inferred from electronic annotation. Source: Ensembl

response to corticosterone

Inferred from electronic annotation. Source: Ensembl

response to herbicide

Inferred from electronic annotation. Source: Ensembl

response to starvation

Inferred from electronic annotation. Source: Ensembl

response to zinc ion

Inferred from electronic annotation. Source: Ensembl

synaptic transmission

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

   Cellular_componentclathrin-sculpted monoamine transport vesicle membrane

Traceable author statement. Source: Reactome

dense core granule

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

membrane

Traceable author statement Ref.1. Source: ProtInc

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

synaptic vesicle membrane

Inferred from electronic annotation. Source: Ensembl

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionamine transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

drug binding

Inferred from electronic annotation. Source: Ensembl

monoamine transmembrane transporter activity

Traceable author statement Ref.1. Source: ProtInc

serotonin transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Synaptic vesicular amine transporter
PRO_0000127514

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Topological domain42 – 12988Lumenal, vesicle Potential
Transmembrane130 – 15021Helical; Potential
Topological domain151 – 1599Cytoplasmic Potential
Transmembrane160 – 18021Helical; Potential
Topological domain181 – 1899Lumenal, vesicle Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 2199Cytoplasmic Potential
Transmembrane220 – 24223Helical; Potential
Topological domain243 – 2486Lumenal, vesicle Potential
Transmembrane249 – 27123Helical; Potential
Topological domain272 – 29120Cytoplasmic Potential
Transmembrane292 – 31120Helical; Potential
Topological domain312 – 32817Lumenal, vesicle Potential
Transmembrane329 – 35224Helical; Potential
Topological domain353 – 3575Cytoplasmic Potential
Transmembrane358 – 37821Helical; Potential
Topological domain379 – 38911Lumenal, vesicle Potential
Transmembrane390 – 41021Helical; Potential
Topological domain411 – 4144Cytoplasmic Potential
Transmembrane415 – 43521Helical; Potential
Topological domain436 – 4405Lumenal, vesicle Potential
Transmembrane441 – 46222Helical; Potential
Topological domain463 – 51452Cytoplasmic Potential

Amino acid modifications

Modified residue5111Phosphoserine By similarity
Modified residue5131Phosphoserine By similarity
Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation911N-linked (GlcNAc...) Potential
Disulfide bond117 ↔ 324 Ref.10

Experimental info

Sequence conflict171R → H in CAA50489. Ref.4
Sequence conflict3021C → S in AAA59877. Ref.1
Sequence conflict3541K → T in AAA59877. Ref.1
Sequence conflict3781A → P in AAA59877. Ref.1
Sequence conflict3951I → N in AAA59877. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q05940 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 42EA91A225485DB2

FASTA51455,713
        10         20         30         40         50         60 
MALSELALVR WLQESRRSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS IKHEKNATEI 

        70         80         90        100        110        120 
QTARPVHTAS ISDSFQSIFS YYDNSTMVTG NATRDLTLHQ TATQHMVTNA SAVPSDCPSE 

       130        140        150        160        170        180 
DKDLLNENVQ VGLLFASKAT VQLITNPFIG LLTNRIGYPI PIFAGFCIMF VSTIMFAFSS 

       190        200        210        220        230        240 
SYAFLLIARS LQGIGSSCSS VAGMGMLASV YTDDEERGNV MGIALGGLAM GVLVGPPFGS 

       250        260        270        280        290        300 
VLYEFVGKTA PFLVLAALVL LDGAIQLFVL QPSRVQPESQ KGTPLTTLLK DPYILIAAGS 

       310        320        330        340        350        360 
ICFANMGIAM LEPALPIWMM ETMCSRKWQL GVAFLPASIS YLIGTNIFGI LAHKMGRWLC 

       370        380        390        400        410        420 
ALLGMIIVGV SILCIPFAKN IYGLIAPNFG VGFAIGMVDS SMMPIMGYLV DLRHVSVYGS 

       430        440        450        460        470        480 
VYAIADVAFC MGYAIGPSAG GAIAKAIGFP WLMTIIGIID ILFAPLCFFL RSPPAKEEKM 

       490        500        510 
AILMDHNCPI KTKMYTQNNI QSYPIGEDEE SESD 

« Hide

References

« Hide 'large scale' references
[1]"A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs."
Surratt C.K., Persico A.M., Yang X.D., Edgar S.R., Bird G.S., Hawkins A.L., Griffin C.A., Li X., Jabs E.W., Uhl G.R.
FEBS Lett. 318:325-330(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Functional identification and molecular cloning of a human brain vesicle monoamine transporter."
Erickson J.D., Eiden L.E.
J. Neurochem. 61:2314-2317(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Chromosomal localization of the human vesicular amine transporter genes."
Peter D., Finn J.P., Klisak I., Liu Y., Kojis T., Heinzmann C., Roghani A., Sparkes R.S., Edwards R.H.
Genomics 18:720-723(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Substantia nigra.
[4]"Extensive sequence divergence between the human and rat brain vesicular monoamine transporter: possible molecular basis for species differences in the susceptibility to MPP+."
Lesch K.P., Gross J., Wolozin B.L., Murphy D.L., Riederer P.
J. Neural Transm. 93:75-82(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[5]"Exon/intron boundaries, novel polymorphisms, and association analysis with schizophrenia of the human synaptic vesicle monoamine transporter (SVMT) gene."
Kunugi H., Ishida S., Akahane A., Nanko S.
Mol. Psychiatry 6:456-460(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[7]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]"Identification of human vesicle monoamine transporter (VMAT2) lumenal cysteines that form an intramolecular disulfide bond."
Thiriot D.S., Sievert M.K., Ruoho A.E.
Biochemistry 41:6346-6353(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BOND.
[11]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
+Additional computationally mapped references.

Web resources

Wikipedia

Vesicular monoamine transporter entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L09118 mRNA. Translation: AAA59877.1.
L23205 mRNA. Translation: AAA61290.1.
L14269 mRNA. Translation: AAA91853.1.
X71354 mRNA. Translation: CAA50489.1.
AB044401 Genomic DNA. Translation: BAB19009.1. Sequence problems.
AK314997 mRNA. Translation: BAG37493.1.
AL391988, AL731557 Genomic DNA. Translation: CAH70348.1.
AL731557, AL391988 Genomic DNA. Translation: CAI14828.1.
CH471066 Genomic DNA. Translation: EAW49428.1.
CH471066 Genomic DNA. Translation: EAW49429.1.
BC108927 mRNA. Translation: AAI08928.1.
BC108928 mRNA. Translation: AAI08929.1.
CCDSCCDS7599.1.
PIRA49368.
S29810.
RefSeqNP_003045.2. NM_003054.4.
UniGeneHs.596992.

3D structure databases

ProteinModelPortalQ05940.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112459. 2 interactions.
STRING9606.ENSP00000298472.

Chemistry

BindingDBQ05940.
ChEMBLCHEMBL1893.
DrugBankDB00386. Alseroxylon.
DB00206. Reserpine.
DB04844. Tetrabenazine.
GuidetoPHARMACOLOGY1012.

Protein family/group databases

TCDB2.A.1.2.29. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteQ05940.

Polymorphism databases

DMDM1722742.

Proteomic databases

PaxDbQ05940.
PRIDEQ05940.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000298472; ENSP00000298472; ENSG00000165646.
GeneID6571.
KEGGhsa:6571.
UCSCuc001ldd.2. human.

Organism-specific databases

CTD6571.
GeneCardsGC10P119000.
H-InvDBHIX0035636.
HGNCHGNC:10935. SLC18A2.
HPAHPA016856.
MIM193001. gene.
neXtProtNX_Q05940.
Orphanet352649. Brain dopamine-serotonin vesicular transport disease.
PharmGKBPA325.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG255549.
HOGENOMHOG000018545.
HOVERGENHBG055082.
InParanoidQ05940.
KOK08155.
OMARIGYQIP.
OrthoDBEOG79CXZ7.
PhylomeDBQ05940.
TreeFamTF313494.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_20679. Amine compound SLC transporters.

Gene expression databases

BgeeQ05940.
CleanExHS_SLC18A2.
GenevestigatorQ05940.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 2 hits.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiVesicular_monoamine_transporter_2.
GenomeRNAi6571.
NextBio25567.
PROQ05940.
SOURCESearch...

Entry information

Entry nameVMAT2_HUMAN
AccessionPrimary (citable) accession number: Q05940
Secondary accession number(s): B2RC96 expand/collapse secondary AC list , D3DRC4, Q15876, Q5VW49, Q9H3P6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM