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Reviewed, UniProtKB/Swiss-Prot Q05940 (VMAT2_HUMAN)

Last modified November 25, 2008. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Synaptic vesicular amine transporter
Alternative name(s):
    Vesicular amine transporter 2
      Short name=VAT2
    Monoamine transporter
    Solute carrier family 18 member 2
Gene names
Name: SLC18A2
Synonyms: SVMT, VMAT2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

Sequence caution

The sequence BAB19009.1 differs from that shown. Reason: Erroneous gene model prediction.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Synaptic vesicular amine transporter
PRO_0000127514

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121 Potential
Topological domain42 – 12988Lumenal, vesicle Potential
Transmembrane130 – 15021 Potential
Topological domain151 – 1599Cytoplasmic Potential
Transmembrane160 – 18021 Potential
Topological domain181 – 1899Lumenal, vesicle Potential
Transmembrane190 – 21021 Potential
Topological domain211 – 2199Cytoplasmic Potential
Transmembrane220 – 24223 Potential
Topological domain243 – 2486Lumenal, vesicle Potential
Transmembrane249 – 27123 Potential
Topological domain272 – 29120Cytoplasmic Potential
Transmembrane292 – 31120 Potential
Topological domain312 – 32817Lumenal, vesicle Potential
Transmembrane329 – 35224 Potential
Topological domain353 – 3575Cytoplasmic Potential
Transmembrane358 – 37821 Potential
Topological domain379 – 38911Lumenal, vesicle Potential
Transmembrane390 – 41021 Potential
Topological domain411 – 4144Cytoplasmic Potential
Transmembrane415 – 43521 Potential
Topological domain436 – 4405Lumenal, vesicle Potential
Transmembrane441 – 46222 Potential
Topological domain463 – 51452Cytoplasmic Potential

Amino acid modifications

Modified residue5111Phosphoserine
Modified residue5131Phosphoserine
Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation911N-linked (GlcNAc...) Potential
Disulfide bond117 ↔ 324

Experimental info

Sequence conflict171R → H in CAA50489. Ref.4
Sequence conflict3021C → S in AAA59877. Ref.1
Sequence conflict3541K → T in AAA59877. Ref.1
Sequence conflict3781A → P in AAA59877. Ref.1
Sequence conflict3951I → N in AAA59877. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q05940-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 42EA91A225485DB2

FASTA51455,713
        10         20         30         40         50         60 
MALSELALVR WLQESRRSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS IKHEKNATEI 

        70         80         90        100        110        120 
QTARPVHTAS ISDSFQSIFS YYDNSTMVTG NATRDLTLHQ TATQHMVTNA SAVPSDCPSE 

       130        140        150        160        170        180 
DKDLLNENVQ VGLLFASKAT VQLITNPFIG LLTNRIGYPI PIFAGFCIMF VSTIMFAFSS 

       190        200        210        220        230        240 
SYAFLLIARS LQGIGSSCSS VAGMGMLASV YTDDEERGNV MGIALGGLAM GVLVGPPFGS 

       250        260        270        280        290        300 
VLYEFVGKTA PFLVLAALVL LDGAIQLFVL QPSRVQPESQ KGTPLTTLLK DPYILIAAGS 

       310        320        330        340        350        360 
ICFANMGIAM LEPALPIWMM ETMCSRKWQL GVAFLPASIS YLIGTNIFGI LAHKMGRWLC 

       370        380        390        400        410        420 
ALLGMIIVGV SILCIPFAKN IYGLIAPNFG VGFAIGMVDS SMMPIMGYLV DLRHVSVYGS 

       430        440        450        460        470        480 
VYAIADVAFC MGYAIGPSAG GAIAKAIGFP WLMTIIGIID ILFAPLCFFL RSPPAKEEKM 

       490        500        510 
AILMDHNCPI KTKMYTQNNI QSYPIGEDEE SESD

« Hide

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References

« Hide 'large scale' references
[1]"A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs."
Surratt C.K., Persico A.M., Yang X.D., Edgar S.R., Bird G.S., Hawkins A.L., Griffin C.A., Li X., Jabs E.W., Uhl G.R.
FEBS Lett. 318:325-330(1993) [PubMed: 8095030] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Functional identification and molecular cloning of a human brain vesicle monoamine transporter."
Erickson J.D., Eiden L.E.
J. Neurochem. 61:2314-2317(1993) [PubMed: 8245983] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Chromosomal localization of the human vesicular amine transporter genes."
Peter D., Finn J.P., Klisak I., Liu Y., Kojis T., Heinzmann C., Roghani A., Sparkes R.S., Edwards R.H.
Genomics 18:720-723(1993) [PubMed: 7905859] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Substantia nigra.
[4]"Extensive sequence divergence between the human and rat brain vesicular monoamine transporter: possible molecular basis for species differences in the usceptibility to MPP+."
Lesch K.P., Gross J., Wolozin B.L., Murphy D.L., Riederer P.
J. Neural Transm. 93:75-82(1993) [PubMed: 8373557] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[5]"Exon/intron boundaries, novel polymorphisms, and association analysis with schizophrenia of the human synaptic vesicle monoamine transporter (SVMT) gene."
Kunugi H., Ishida S., Akahane A., Nanko S.
Mol. Psychiatry 6:456-460(2001) [PubMed: 11443533] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed: 15164054] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Identification of human vesicle monoamine transporter (VMAT2) lumenal cysteines that form an intramolecular disulfide bond."
Thiriot D.S., Sievert M.K., Ruoho A.E.
Biochemistry 41:6346-6353(2002) [PubMed: 12009896] [Abstract]
Cited for: DISULFIDE BOND.
[9]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-511 AND SER-513, MASS SPECTROMETRY.
Tissue: Platelet.
+Additional computationally mapped references.

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Web resources

Wikipedia

Vesicular monoamine transporter entry

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Cross-references

Sequence databases

L09118 mRNA. Translation: AAA59877.1.
L23205 mRNA. Translation: AAA61290.1.
L14269 mRNA. Translation: AAA91853.1.
X71354 mRNA. Translation: CAA50489.1.
AB044401 Genomic DNA. Translation: BAB19009.1. Sequence problems.
AL391988, AL731557 Genomic DNA. Translation: CAH70348.1.
AL731557, AL391988 Genomic DNA. Translation: CAI14828.1.
BC108927 mRNA. Translation: AAI08928.1.
BC108928 mRNA. Translation: AAI08929.1.
PIRA49368.
S29810.
RefSeqNP_003045.2.
UniGeneHs.654476

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteQ05940.

Genome annotation databases

EnsemblENSG00000165646. Homo sapiens. [Contig view]
GeneID6571.
KEGGhsa:6571.

Organism-specific databases

H-InvDBHIX0035382.
HIX0035636.
HGNCHGNC:10935. SLC18A2.
HPAHPA016856.
MIM193001. gene.
PharmGKBPA325.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ05940.
HOVERGENQ05940.

Gene expression databases

ArrayExpressQ05940.
CleanExHS_SLC18A2.
GermOnlineENSG00000165646. Homo sapiens.

Family and domain databases

InterProIPR007114. MFS.
IPR011701. MFS_1.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00386. Alseroxylon.
DB00206. Reserpine.
DB04844. Tetrabenazine.
NextBio25567.
SOURCESearch...

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Entry information

Entry nameVMAT2_HUMAN
AccessionPrimary (citable) accession number: Q05940
Secondary accession number(s): Q15876, Q5VW49, Q9H3P6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: November 25, 2008
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents