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Protein

Synaptic vesicular amine transporter

Gene

SLC18A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

GO - Molecular functioni

GO - Biological processi

  • aminergic neurotransmitter loading into synaptic vesicle Source: GO_Central
  • chemical synaptic transmission Source: ParkinsonsUK-UCL
  • dopamine transport Source: ParkinsonsUK-UCL
  • locomotory behavior Source: Ensembl
  • monoamine transport Source: ProtInc
  • negative regulation of reactive oxygen species biosynthetic process Source: ParkinsonsUK-UCL
  • neurotransmitter loading into synaptic vesicle Source: ParkinsonsUK-UCL
  • neurotransmitter secretion Source: Reactome
  • post-embryonic development Source: Ensembl
  • response to amphetamine Source: Ensembl
  • response to toxic substance Source: Ensembl
  • sequestering of neurotransmitter Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000165646-MONOMER.
ReactomeiR-HSA-181429. Serotonin Neurotransmitter Release Cycle.
R-HSA-181430. Norepinephrine Neurotransmitter Release Cycle.
R-HSA-212676. Dopamine Neurotransmitter Release Cycle.
R-HSA-442660. Na+/Cl- dependent neurotransmitter transporters.
SIGNORiQ05940.

Protein family/group databases

TCDBi2.A.1.2.29. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:SLC18A2
Synonyms:SVMT, VMAT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:10935. SLC18A2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 129Lumenal, vesicleSequence analysisAdd BLAST88
Transmembranei130 – 150HelicalSequence analysisAdd BLAST21
Topological domaini151 – 159CytoplasmicSequence analysis9
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 189Lumenal, vesicleSequence analysis9
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 219CytoplasmicSequence analysis9
Transmembranei220 – 242HelicalSequence analysisAdd BLAST23
Topological domaini243 – 248Lumenal, vesicleSequence analysis6
Transmembranei249 – 271HelicalSequence analysisAdd BLAST23
Topological domaini272 – 291CytoplasmicSequence analysisAdd BLAST20
Transmembranei292 – 311HelicalSequence analysisAdd BLAST20
Topological domaini312 – 328Lumenal, vesicleSequence analysisAdd BLAST17
Transmembranei329 – 352HelicalSequence analysisAdd BLAST24
Topological domaini353 – 357CytoplasmicSequence analysis5
Transmembranei358 – 378HelicalSequence analysisAdd BLAST21
Topological domaini379 – 389Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Topological domaini411 – 414CytoplasmicSequence analysis4
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Topological domaini436 – 440Lumenal, vesicleSequence analysis5
Transmembranei441 – 462HelicalSequence analysisAdd BLAST22
Topological domaini463 – 514CytoplasmicSequence analysisAdd BLAST52

GO - Cellular componenti

  • clathrin-sculpted monoamine transport vesicle membrane Source: Reactome
  • integral component of plasma membrane Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: Reactome
  • synaptic vesicle Source: ParkinsonsUK-UCL
  • synaptic vesicle membrane Source: GO_Central
  • terminal bouton Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6571.
MalaCardsiSLC18A2.
OpenTargetsiENSG00000165646.
Orphaneti352649. Brain dopamine-serotonin vesicular transport disease.
PharmGKBiPA325.

Chemistry databases

ChEMBLiCHEMBL1893.
DrugBankiDB00182. Amphetamine.
DB00865. Benzphetamine.
DB01089. Deserpidine.
DB01576. Dextroamphetamine.
DB01363. Ephedra.
DB01364. Ephedrine.
DB06706. Isometheptene.
DB01577. Methamphetamine.
DB00368. Norepinephrine.
DB06714. Propylhexedrine.
DB00206. Reserpine.
DB04844. Tetrabenazine.
GuidetoPHARMACOLOGYi1012.

Polymorphism and mutation databases

BioMutaiSLC18A2.
DMDMi1722742.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275141 – 514Synaptic vesicular amine transporterAdd BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi91N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi117 ↔ 3241 Publication
Modified residuei511PhosphoserineBy similarity1
Modified residuei513PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ05940.
PaxDbiQ05940.
PeptideAtlasiQ05940.
PRIDEiQ05940.

PTM databases

iPTMnetiQ05940.
PhosphoSitePlusiQ05940.

Expressioni

Gene expression databases

BgeeiENSG00000165646.
CleanExiHS_SLC18A2.
GenevisibleiQ05940. HS.

Organism-specific databases

HPAiHPA016856.

Interactioni

Subunit structurei

Interacts with SLC6A3.By similarity

Protein-protein interaction databases

BioGridi112459. 1 interactor.
STRINGi9606.ENSP00000298472.

Chemistry databases

BindingDBiQ05940.

Structurei

3D structure databases

ProteinModelPortaliQ05940.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ05940.
KOiK08155.
OMAiQKAETEP.
OrthoDBiEOG091G0534.
PhylomeDBiQ05940.
TreeFamiTF313494.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q05940-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALSELALVR WLQESRRSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS
60 70 80 90 100
IKHEKNATEI QTARPVHTAS ISDSFQSIFS YYDNSTMVTG NATRDLTLHQ
110 120 130 140 150
TATQHMVTNA SAVPSDCPSE DKDLLNENVQ VGLLFASKAT VQLITNPFIG
160 170 180 190 200
LLTNRIGYPI PIFAGFCIMF VSTIMFAFSS SYAFLLIARS LQGIGSSCSS
210 220 230 240 250
VAGMGMLASV YTDDEERGNV MGIALGGLAM GVLVGPPFGS VLYEFVGKTA
260 270 280 290 300
PFLVLAALVL LDGAIQLFVL QPSRVQPESQ KGTPLTTLLK DPYILIAAGS
310 320 330 340 350
ICFANMGIAM LEPALPIWMM ETMCSRKWQL GVAFLPASIS YLIGTNIFGI
360 370 380 390 400
LAHKMGRWLC ALLGMIIVGV SILCIPFAKN IYGLIAPNFG VGFAIGMVDS
410 420 430 440 450
SMMPIMGYLV DLRHVSVYGS VYAIADVAFC MGYAIGPSAG GAIAKAIGFP
460 470 480 490 500
WLMTIIGIID ILFAPLCFFL RSPPAKEEKM AILMDHNCPI KTKMYTQNNI
510
QSYPIGEDEE SESD
Length:514
Mass (Da):55,713
Last modified:October 1, 1996 - v2
Checksum:i42EA91A225485DB2
GO
Isoform 2 (identifier: Q05940-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     204-209: MGMLAS → RCGMPE
     210-514: Missing.

Note: No experimental confirmation available.
Show »
Length:209
Mass (Da):22,980
Checksum:iE5C6638E4F86FF66
GO

Sequence cautioni

The sequence BAB19009 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17R → H in CAA50489 (PubMed:8373557).Curated1
Sequence conflicti302C → S in AAA59877 (PubMed:8095030).Curated1
Sequence conflicti354K → T in AAA59877 (PubMed:8095030).Curated1
Sequence conflicti378A → P in AAA59877 (PubMed:8095030).Curated1
Sequence conflicti395I → N in AAA59877 (PubMed:8095030).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057151204 – 209MGMLAS → RCGMPE in isoform 2. 1 Publication6
Alternative sequenceiVSP_057152210 – 514Missing in isoform 2. 1 PublicationAdd BLAST305

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09118 mRNA. Translation: AAA59877.1.
L23205 mRNA. Translation: AAA61290.1.
L14269 mRNA. Translation: AAA91853.1.
X71354 mRNA. Translation: CAA50489.1.
AB044401 Genomic DNA. Translation: BAB19009.1. Sequence problems.
AK314997 mRNA. Translation: BAG37493.1.
AL391988, AL731557 Genomic DNA. Translation: CAH70348.1.
AL731557, AL391988 Genomic DNA. Translation: CAI14828.1.
CH471066 Genomic DNA. Translation: EAW49428.1.
CH471066 Genomic DNA. Translation: EAW49429.1.
BC030593 mRNA. Translation: AAH30593.1.
BC108927 mRNA. Translation: AAI08928.1.
BC108928 mRNA. Translation: AAI08929.1.
CCDSiCCDS7599.1. [Q05940-1]
PIRiA49368.
S29810.
RefSeqiNP_003045.2. NM_003054.4. [Q05940-1]
UniGeneiHs.596992.

Genome annotation databases

EnsembliENST00000298472; ENSP00000298472; ENSG00000165646. [Q05940-1]
GeneIDi6571.
KEGGihsa:6571.
UCSCiuc001ldd.3. human. [Q05940-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Wikipedia

Vesicular monoamine transporter entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09118 mRNA. Translation: AAA59877.1.
L23205 mRNA. Translation: AAA61290.1.
L14269 mRNA. Translation: AAA91853.1.
X71354 mRNA. Translation: CAA50489.1.
AB044401 Genomic DNA. Translation: BAB19009.1. Sequence problems.
AK314997 mRNA. Translation: BAG37493.1.
AL391988, AL731557 Genomic DNA. Translation: CAH70348.1.
AL731557, AL391988 Genomic DNA. Translation: CAI14828.1.
CH471066 Genomic DNA. Translation: EAW49428.1.
CH471066 Genomic DNA. Translation: EAW49429.1.
BC030593 mRNA. Translation: AAH30593.1.
BC108927 mRNA. Translation: AAI08928.1.
BC108928 mRNA. Translation: AAI08929.1.
CCDSiCCDS7599.1. [Q05940-1]
PIRiA49368.
S29810.
RefSeqiNP_003045.2. NM_003054.4. [Q05940-1]
UniGeneiHs.596992.

3D structure databases

ProteinModelPortaliQ05940.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112459. 1 interactor.
STRINGi9606.ENSP00000298472.

Chemistry databases

BindingDBiQ05940.
ChEMBLiCHEMBL1893.
DrugBankiDB00182. Amphetamine.
DB00865. Benzphetamine.
DB01089. Deserpidine.
DB01576. Dextroamphetamine.
DB01363. Ephedra.
DB01364. Ephedrine.
DB06706. Isometheptene.
DB01577. Methamphetamine.
DB00368. Norepinephrine.
DB06714. Propylhexedrine.
DB00206. Reserpine.
DB04844. Tetrabenazine.
GuidetoPHARMACOLOGYi1012.

Protein family/group databases

TCDBi2.A.1.2.29. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ05940.
PhosphoSitePlusiQ05940.

Polymorphism and mutation databases

BioMutaiSLC18A2.
DMDMi1722742.

Proteomic databases

EPDiQ05940.
PaxDbiQ05940.
PeptideAtlasiQ05940.
PRIDEiQ05940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298472; ENSP00000298472; ENSG00000165646. [Q05940-1]
GeneIDi6571.
KEGGihsa:6571.
UCSCiuc001ldd.3. human. [Q05940-1]

Organism-specific databases

CTDi6571.
DisGeNETi6571.
GeneCardsiSLC18A2.
H-InvDBHIX0035636.
HGNCiHGNC:10935. SLC18A2.
HPAiHPA016856.
MalaCardsiSLC18A2.
MIMi193001. gene.
neXtProtiNX_Q05940.
OpenTargetsiENSG00000165646.
Orphaneti352649. Brain dopamine-serotonin vesicular transport disease.
PharmGKBiPA325.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ05940.
KOiK08155.
OMAiQKAETEP.
OrthoDBiEOG091G0534.
PhylomeDBiQ05940.
TreeFamiTF313494.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000165646-MONOMER.
ReactomeiR-HSA-181429. Serotonin Neurotransmitter Release Cycle.
R-HSA-181430. Norepinephrine Neurotransmitter Release Cycle.
R-HSA-212676. Dopamine Neurotransmitter Release Cycle.
R-HSA-442660. Na+/Cl- dependent neurotransmitter transporters.
SIGNORiQ05940.

Miscellaneous databases

GeneWikiiVesicular_monoamine_transporter_2.
GenomeRNAii6571.
PROiQ05940.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165646.
CleanExiHS_SLC18A2.
GenevisibleiQ05940. HS.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVMAT2_HUMAN
AccessioniPrimary (citable) accession number: Q05940
Secondary accession number(s): B2RC96
, D3DRC4, Q15876, Q4G147, Q5VW49, Q9H3P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.