Q05936 (SCRB_STAXY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sucrose-6-phosphate hydrolase
Short name=Sucrase
EC=3.2.1.26

Alternative name(s):
Invertase

Gene names

Name:

scrB

Organism

Staphylococcus xylosus

Taxonomic identifier

1288 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Sucrose-6-phosphate hydrolase		PRO_0000169875
Regions
	Region	45 – 48	4	Substrate binding By similarity
	Region	107 – 108	2	Substrate binding By similarity
	Region	168 – 169	2	Substrate binding By similarity
Sites
	Active site	48	1	By similarity
	Binding site	64	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05936 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 3F129C33DC1DAB78	FASTA	494	57,371
	10 20 30 40 50 60 MSEWTKEQRY QKYEDVDQQT IEKLTEQVNQ SEYRQTFHIQ PQIGLLNDPN GLIYFKGNYY 70 80 90 100 110 120 VSHQWFPLGP VHGLKYWYTY QSKDLVDFKA IGPTIKPDTK DDSHGVYSGS AFEYHDHLYY 130 140 150 160 170 180 MYTANHRDSD WNRISTQHIA KMSKDGSINK FPKAVISAPP SGYTQHFRDP KVHVQDGVYY 190 200 210 220 230 240 AMIAAQNIKK QGRILQYRST DIVNWEFQGE VQTNLDDFGY MWECPDYFNL NGYDMLLFCP 250 260 270 280 290 300 QGIDSEGERF KNIYQSGYIM GQYDINNLTM NHADFHELDY GFDFYAPQTF LDENGQRILI 310 320 330 340 350 360 GWMGLPDINY PSDADGWAHC LTIPRVLTIE SGNLKQRPIK ALEKLRTNEE TALGYANKFT 370 380 390 400 410 420 RQLHPYEGKQ FELIIDILEN EATEVYFEVR TSKTESTLIT YNKREQKLTL DRSESGQLPE 430 440 450 460 470 480 PVEGTTRSTY LDTPLSKLQL FVDTSSVEIF CNDGERVMTA RIFTDENATG IKTSTESGQT 490 YLKFTKYDLK GDTI

References

[1]	"Characterization of a sucrase gene from Staphylococcus xylosus." Brueckner R., Wagner E., Goetz F. J. Bacteriol. 175:851-857(1993) [PubMed: 8423155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 20267 / Isolate C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67744 Genomic DNA. Translation: CAA47970.1.
PIR	A47059.
3D structure databases
ProteinModelPortal	Q05936.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12602.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. IPR006232. Suc6P_hydrolase. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
TIGRFAMs	TIGR01322. ScrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_STAXY

Accession

Primary (citable) accession number: Q05936

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 21, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program