Q05933 (ARPC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin-related protein 2/3 complex subunit 3

Alternative name(s):
Arp2/3 complex 21 kDa subunit
Short name=p21-ARC

Gene names

Name:	ARC18
Ordered Locus Names:	YLR370C
ORF Names:	L8039.15

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks By similarity.
Subunit structure	Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC.
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the ARPC3 family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin filament organization Inferred from mutant phenotype. Source: SGD establishment of mitochondrion localization Inferred from mutant phenotype. Source: SGD mitochondrion inheritance Traceable author statement. Source: SGD regulation of actin filament polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	Arp2/3 protein complex Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARC35	P53731	5	EBI-2764,EBI-2770
ARC40	P38328	4	EBI-2764,EBI-2777

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Actin-related protein 2/3 complex subunit 3		PRO_0000124048

Sequences

	Sequence		Length	Mass (Da)
	Q05933 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 456EABC2B9CC4860	FASTA	178	20,579
	10 20 30 40 50 60 MPAYHSTFPV DPNTDRMVGN FALLPLNTKF RGPAYPSNSD YDIIDECLDL FRANSFFKNF 70 80 90 100 110 120 EIKSPADRVL IYGILFINDC LAHLKITTSF NEAVKVLTNV ALDNFTLPGT PGFPLNNVYQ 130 140 150 160 170 VPVQDHNSMD LLKTYIQQFR QELAMRLLER VYSSTDSKEY PSKFWLAFTR RRFMNKSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches." Winter D., Podtelejnikov A.V., Mann M., Li R. Curr. Biol. 7:519-529(1997) [PubMed: 9210376] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX.
[5]	Erratum Winter D., Podtelejnikov A.V., Mann M., Li R. Curr. Biol. 7:R593-R593(1997)
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19103 Genomic DNA. Translation: AAB67576.1. AY558319 Genomic DNA. Translation: AAS56645.1. BK006945 Genomic DNA. Translation: DAA09673.1.
PIR	S51388.
RefSeq	NP_013474.1. NM_001182259.1.
3D structure databases
ProteinModelPortal	Q05933.
SMR	Q05933. Positions 2-178.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2218N.
IntAct	Q05933. 21 interactions.
MINT	MINT-570288.
STRING	Q05933.
Proteomic databases
PeptideAtlas	Q05933.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR370C; YLR370C; YLR370C.
GeneID	851085.
KEGG	sce:YLR370C.
NMPDR	fig|4932.3.peg.4499.
Organism-specific databases
CYGD	YLR370c.
SGD	S000004362. ARC18.
Phylogenomic databases
eggNOG	fuNOG08411.
GeneTree	EFGT00050000006570.
HOGENOM	HBG320336.
OMA	KWWICFA.
OrthoDB	EOG4KSSV1.
Gene expression databases
ArrayExpress	Q05933.
Genevestigator	Q05933.
GermOnline	YLR370C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007204. ARP2/3_p21-Arc. [Graphical view]
Gene3D	G3DSA:1.10.1760.10. ARP2/3_P21-Arc. 1 hit.
KO	K05756.
PANTHER	PTHR12391. P21-Arc. 1 hit.
Pfam	PF04062. P21-Arc. 1 hit. [Graphical view]
PIRSF	PIRSF016315. ARP2/3_P21-Arc. 1 hit.
SUPFAM	SSF69060. ARP2/3_P21-Arc. 1 hit.
ProtoNet	Search...
Other
NextBio	967750.

Entry information

Entry name

ARPC3_YEAST

Accession

Primary (citable) accession number: Q05933
Secondary accession number(s): D6VZ07

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program