Reviewed, UniProtKB/Swiss-Prot Q05930 (MDM30_YEAST)

Names and origin

Protein names

Recommended name:
    Mitochondrial distribution and morphology protein 30

Gene names

Name:	MDM30
Ordered Locus Names:	YLR368W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity. Seems to recognize FZ01 and regulates the amount of FZO1. Regulatory factor for the mitochondrial fusion machinery. Required for mitochondrial DNA maintenance.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Component of the probable SCF(MDM30) complex containing CDC53, SKP1, RBX1 and MDM30. Interacts with SKP1. Ref.5
Subcellular location	Cytoplasm. Mitochondrion. Ref.3 Ref.4
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Mitochondrion
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: SGD chronological cell aging Inferred from mutant phenotype. Source: SGD mitochondrial fusion Ref.3 Inferred from mutant phenotype. Source: SGD protein ubiquitination during ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: SGD regulation of mRNA export from nucleus Inferred from mutant phenotype. Source: SGD
Cellular component	SCF ubiquitin ligase complex Inferred from physical interaction. Source: SGD mitochondrion Ref.4 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC53	Q12018	2	EBI-31799,EBI-4321
ECM4	P36156	1	EBI-31799,EBI-2042717
IMP2	P46972	1	EBI-31799,EBI-9231

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	Mitochondrial distribution and morphology protein 30		PRO_0000119969
Regions
	Domain	13 – 59	47	F-box

Sequences

	Sequence		Length	Mass (Da)
	Q05930-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CECDC2E2C7EDBB06	FASTA	598	70,528
	10 20 30 40 50 60 MTKRRNLFMV GSSFTIDHLP PEIWLCISKL VGTSDLHNLC LINRRLYLTI TSDEIWKRRC 70 80 90 100 110 120 YDRWINRESL DILTGNDYDS IPVSQWYSYY LRRAKWENKI FCLLWGLTEE TNPQHFREKY 130 140 150 160 170 180 LHILQFRHYK LATFLHRIIK QGYIPDKRPL DLITYANYLL KNVRHKYVFP LFYPTNAAEL 190 200 210 220 230 240 KNLNNMASRD AEMIYLRLSA IDTSFDDLLD AREFILNGIC SDLLQKYKKI EEFLKLRPVT 250 260 270 280 290 300 RVSKLISIST DYLDCFTQPH DSVGQTNDRA TGRELHREDF MLLRVYSREG RGYKTIILAI 310 320 330 340 350 360 IQAITKRYNV DSYLARDHLV VSEPDFPDGR AFVTVNEDFQ PYIFDKEDLL SVWSNNFHNA 370 380 390 400 410 420 ENFESTVLPA LLEPISIQHL LTEFFRELLR CKPRPFEGYP NRAHGLRDMF PYGKVEVPRD 430 440 450 460 470 480 VTMYFAFIYD LFDGMFESGM TSLRGQMLRD LLNYVNANNF GDLNIIIGQN ALKEPNDCWS 490 500 510 520 530 540 NKRDYVLLDD NNKIGYFYTD IETEDTLCAL NQYEVDGKVF ITTIDILGDI RVRLAEGLTP 550 560 570 580 590 FQGDNDKLWE SFSSVVPRTD WGLFFKGYDK ERRRMQLNPY IEEKLSNLAN DEQPLHNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae." Dimmer K.S., Fritz S., Fuchs F., Messerschmitt M., Weinbach N., Neupert W., Westermann B. Mol. Biol. Cell 13:847-853(2002) [PubMed: 11907266] [Abstract] Cited for: FUNCTION.
[3]	"Mdm30 is an F-box protein required for maintenance of fusion-competent mitochondria in yeast." Fritz S., Weinbach N., Westermann B. Mol. Biol. Cell 14:2303-2313(2003) [PubMed: 12808031] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro." Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M. Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract] Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(MDM30) COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19103 Genomic DNA. Translation: AAB67566.1.
PIR	S51386.
RefSeq	NP_013472.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1239N.
IntAct	Q05930. 11 interactions.
Genome annotation databases
Ensembl	YLR368W. Saccharomyces cerevisiae. [Contig view]
GeneID	851083.
GenomeReviews	Gene locus YLR368W in contig Y13138_GR.
KEGG	sce:YLR368W.
NMPDR	fig|4932.3.peg.4497.
Organism-specific databases
CYGD	YLR368w.
SGD	S000004360. MDM30.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q05930.
OMA	Q05930. FRESICE.
Gene expression databases
GermOnline	YLR368W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967744.

Entry information

Entry name

MDM30_YEAST

Accession

Primary (citable) accession number: Q05930

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)