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Protein

Mitochondrial distribution and morphology protein 30

Gene

MDM30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins (By similarity). Recognizes FZO1 and regulates the amount of FZO1. Regulatory factor for the mitochondrial fusion machinery. Required for mitochondrial DNA maintenance.By similarity6 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • chronological cell aging Source: SGD
  • mitochondrial fusion Source: SGD
  • mitochondrion organization Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • proteolysis involved in cellular protein catabolic process Source: SGD
  • regulation of mRNA export from nucleus Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32437-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial distribution and morphology protein 30
Gene namesi
Name:MDM30
Ordered Locus Names:YLR368W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR368W.
SGDiS000004360. MDM30.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB-SubCell
  • SCF ubiquitin ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Mitochondrial distribution and morphology protein 30PRO_0000119969Add
BLAST

Proteomic databases

PRIDEiQ05930.

Interactioni

Subunit structurei

Interacts with SKP1. Component of the probable SCF(MDM30) complex containing CDC53, SKP1, RBX1 and MDM30. Interacts with SKP1 and FZO1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IMP2P469722EBI-31799,EBI-9231
SKP1P522863EBI-31799,EBI-4090

Protein-protein interaction databases

BioGridi31628. 48 interactions.
DIPiDIP-1239N.
IntActiQ05930. 11 interactions.
MINTiMINT-384045.

Structurei

3D structure databases

ProteinModelPortaliQ05930.
SMRiQ05930. Positions 14-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 5947F-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ05930.
KOiK15070.
OMAiFRESICE.
OrthoDBiEOG79W9DS.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKRRNLFMV GSSFTIDHLP PEIWLCISKL VGTSDLHNLC LINRRLYLTI
60 70 80 90 100
TSDEIWKRRC YDRWINRESL DILTGNDYDS IPVSQWYSYY LRRAKWENKI
110 120 130 140 150
FCLLWGLTEE TNPQHFREKY LHILQFRHYK LATFLHRIIK QGYIPDKRPL
160 170 180 190 200
DLITYANYLL KNVRHKYVFP LFYPTNAAEL KNLNNMASRD AEMIYLRLSA
210 220 230 240 250
IDTSFDDLLD AREFILNGIC SDLLQKYKKI EEFLKLRPVT RVSKLISIST
260 270 280 290 300
DYLDCFTQPH DSVGQTNDRA TGRELHREDF MLLRVYSREG RGYKTIILAI
310 320 330 340 350
IQAITKRYNV DSYLARDHLV VSEPDFPDGR AFVTVNEDFQ PYIFDKEDLL
360 370 380 390 400
SVWSNNFHNA ENFESTVLPA LLEPISIQHL LTEFFRELLR CKPRPFEGYP
410 420 430 440 450
NRAHGLRDMF PYGKVEVPRD VTMYFAFIYD LFDGMFESGM TSLRGQMLRD
460 470 480 490 500
LLNYVNANNF GDLNIIIGQN ALKEPNDCWS NKRDYVLLDD NNKIGYFYTD
510 520 530 540 550
IETEDTLCAL NQYEVDGKVF ITTIDILGDI RVRLAEGLTP FQGDNDKLWE
560 570 580 590
SFSSVVPRTD WGLFFKGYDK ERRRMQLNPY IEEKLSNLAN DEQPLHNL
Length:598
Mass (Da):70,528
Last modified:November 1, 1996 - v1
Checksum:iCECDC2E2C7EDBB06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19103 Genomic DNA. Translation: AAB67566.1.
BK006945 Genomic DNA. Translation: DAA09671.1.
PIRiS51386.
RefSeqiNP_013472.3. NM_001182257.3.

Genome annotation databases

EnsemblFungiiYLR368W; YLR368W; YLR368W.
GeneIDi851083.
KEGGisce:YLR368W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19103 Genomic DNA. Translation: AAB67566.1.
BK006945 Genomic DNA. Translation: DAA09671.1.
PIRiS51386.
RefSeqiNP_013472.3. NM_001182257.3.

3D structure databases

ProteinModelPortaliQ05930.
SMRiQ05930. Positions 14-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31628. 48 interactions.
DIPiDIP-1239N.
IntActiQ05930. 11 interactions.
MINTiMINT-384045.

Proteomic databases

PRIDEiQ05930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR368W; YLR368W; YLR368W.
GeneIDi851083.
KEGGisce:YLR368W.

Organism-specific databases

EuPathDBiFungiDB:YLR368W.
SGDiS000004360. MDM30.

Phylogenomic databases

InParanoidiQ05930.
KOiK15070.
OMAiFRESICE.
OrthoDBiEOG79W9DS.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-32437-MONOMER.

Miscellaneous databases

NextBioi967744.
PROiQ05930.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae."
    Dimmer K.S., Fritz S., Fuchs F., Messerschmitt M., Weinbach N., Neupert W., Westermann B.
    Mol. Biol. Cell 13:847-853(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Mdm30 is an F-box protein required for maintenance of fusion-competent mitochondria in yeast."
    Fritz S., Weinbach N., Westermann B.
    Mol. Biol. Cell 14:2303-2313(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  6. "Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
    Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.
    Proteins 54:455-467(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(MDM30) COMPLEX.
  7. "Regulation of mitochondrial fusion by the F-box protein Mdm30 involves proteasome-independent turnover of Fzo1."
    Escobar-Henriques M., Westermann B., Langer T.
    J. Cell Biol. 173:645-650(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FZO1.
  8. "Ubiquitin-proteasome-dependent degradation of a mitofusin, a critical regulator of mitochondrial fusion."
    Cohen M.M., Leboucher G.P., Livnat-Levanon N., Glickman M.H., Weissman A.M.
    Mol. Biol. Cell 19:2457-2464(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion."
    Anton F., Fres J.M., Schauss A., Pinson B., Praefcke G.J., Langer T., Escobar-Henriques M.
    J. Cell Sci. 124:1126-1135(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FZO1.
  10. "Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion."
    Cohen M.M., Amiott E.A., Day A.R., Leboucher G.P., Pryce E.N., Glickman M.H., McCaffery J.M., Shaw J.M., Weissman A.M.
    J. Cell Sci. 124:1403-1410(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FZO1.

Entry informationi

Entry nameiMDM30_YEAST
AccessioniPrimary (citable) accession number: Q05930
Secondary accession number(s): D6VZ05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.