Q05928 (BTC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probetacellulin

Cleaved into the following chain:

1. Betacellulin
   Short name=BTC

Gene names

Name:	Btc
Synonyms:	Bcn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Ref.3
Subunit structure	Monomer. Interacts with EGFR and ERBB4.
Subcellular location	Betacellulin: Secreted › extracellular space. Probetacellulin: Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain.
Sequence similarities	Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
Molecular function	Growth factor Mitogen
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of apoptotic process Inferred from direct assay PubMed 18656477. Source: MGI positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell proliferation Inferred from genetic interaction PubMed 18656477. Source: MGI positive regulation of mitosis Inferred from sequence or structural similarity PubMed 11004502. Source: MGI
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from sequence or structural similarity PubMed 11004502. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Ref.1
	Chain	32 – 177	146	Probetacellulin		PRO_0000300686
	Chain	32 – 111	80	Betacellulin		PRO_0000007492
	Propeptide	112 – 177	66	Removed in mature form		PRO_0000007493
Regions
	Topological domain	32 – 118	87	Extracellular Potential
	Transmembrane	119 – 139	21	Helical; Potential
	Topological domain	140 – 177	38	Cytoplasmic Potential
	Domain	65 – 105	41	EGF-like
	Compositional bias	146 – 153	8	Arg/Lys-rich (basic)
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Disulfide bond	69 ↔ 82		By similarity
	Disulfide bond	77 ↔ 93		By similarity
	Disulfide bond	95 ↔ 104		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05928 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 066BB34F0E13F82B	FASTA	177	19,664
	10 20 30 40 50 60 MDPTAPGSSV SSLPLLLVLA LGLAILHCVV ADGNTTRTPE TNGSLCGAPG ENCTGTTPRQ 70 80 90 100 110 120 KVKTHFSRCP KQYKHYCIHG RCRFVVDEQT PSCICEKGYF GARCERVDLF YLQQDRGQIL 130 140 150 160 170 VVCLIVVMVV FIILVIGVCT CCHPLRKHRK KKKEEKMETL DKDKTPISED IQETNIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Betacellulin: a mitogen from pancreatic beta cell tumors." Shing Y., Christofori G., Hanahan D., Ono Y., Sasada R., Igarashi K., Folkman J. Science 259:1604-1607(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-54; 64-71 AND 75-111. Tissue: Pancreas.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Skin.
[3]	"Betacellulin-induced beta cell proliferation and regeneration is mediated by activation of ErbB-1 and ErbB-2 receptors." Oh Y.S., Shin S., Lee Y.J., Kim E.H., Jun H.S. PLoS ONE 6:E23894-E23894(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08394 mRNA. Translation: AAA40511.1. AK076272 mRNA. Translation: BAC36281.1.
IPI	IPI00114721.
PIR	A37408.
RefSeq	NP_031594.1. NM_007568.5.
UniGene	Mm.2024.
3D structure databases
ProteinModelPortal	Q05928.
SMR	Q05928. Positions 63-111.
ModBase	Search...
Proteomic databases
PRIDE	Q05928.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000121044; ENSMUSP00000112765; ENSMUSG00000082361.
GeneID	12223.
KEGG	mmu:12223.
Organism-specific databases
CTD	685.
MGI	MGI:99439. Btc.
Phylogenomic databases
eggNOG	NOG121071.
HOGENOM	HOG000237352.
HOVERGEN	HBG004905.
InParanoid	Q05928.
KO	K09783.
OMA	TQSKRKG.
OrthoDB	EOG4WH8N4.
Gene expression databases
ArrayExpress	Q05928.
Bgee	Q05928.
Genevestigator	Q05928.
GermOnline	ENSMUSG00000029391. Mus musculus.
Family and domain databases
InterPro	IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR015497. EGF_rcpt_ligand. [Graphical view]
PANTHER	PTHR10740. PTHR10740. 1 hit.
Pfam	PF00008. EGF. 1 hit. [Graphical view]
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280627.
PMAP-CutDB	Q05928.
SOURCE	Search...

Entry information

Entry name

BTC_MOUSE

Accession

Primary (citable) accession number: Q05928

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program