Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q05927 (5NTD_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5'-nucleotidase
Short name=5'-NT
EC=3.1.3.5
Alternative name(s):
Ecto-5'-nucleotidase
CD_antigen=CD73
Gene names
Name:NT5E
Synonyms:NT5, NTE
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Catalytic activity

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactor

Zinc.

Subunit structure

Homodimer; disulfide-linked.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor.

Sequence similarities

Belongs to the 5'-nucleotidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626
Chain27 – 5495235'-nucleotidase
PRO_0000000013
Propeptide550 – 57425Removed in mature form By similarity
PRO_0000000014

Regions

Region500 – 5067Substrate binding By similarity

Sites

Metal binding361Zinc 1 By similarity
Metal binding381Zinc 1 By similarity
Metal binding851Zinc 1 By similarity
Metal binding851Zinc 2 By similarity
Metal binding1171Zinc 2 By similarity
Metal binding2201Zinc 2 By similarity
Metal binding2431Zinc 2 By similarity
Binding site4171Substrate By similarity
Site1181Transition state stabilizer By similarity
Site1211Transition state stabilizer By similarity

Amino acid modifications

Lipidation5491GPI-anchor amidated serine By similarity
Glycosylation531N-linked (GlcNAc...) Potential
Glycosylation3111N-linked (GlcNAc...) Potential
Glycosylation3331N-linked (GlcNAc...) Potential
Glycosylation4031N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict151A → P in BAA03408. Ref.1
Sequence conflict151A → P in AAB27698. Ref.1
Sequence conflict111 – 1122DA → ES in BAA03408. Ref.1
Sequence conflict111 – 1122DA → ES in AAB27698. Ref.1
Sequence conflict436 – 4383EHS → DDT in BAA03408. Ref.1
Sequence conflict436 – 4383EHS → DDT in AAB27698. Ref.1
Sequence conflict4451A → R in BAA03408. Ref.1
Sequence conflict4451A → R in AAB27698. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q05927 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: DF5BF74E05BFE0A3

FASTA57462,966
        10         20         30         40         50         60 
MNPGAARTPA LRILALGALL WPAARPWELT ILHTNDVHSR LEQTSEDSSK CVNASRCVGG 

        70         80         90        100        110        120 
VARLATKVHQ IRRAEPHVLL LDAGDQYQGT IWFTVYKGTE VAHFMNALGY DAMALGNHEF 

       130        140        150        160        170        180 
DNGVEGLIDP LLKEVNFPIL SANIKAKGPL ASKISGLYSP YKILTVGDEV VGIVGYTSKE 

       190        200        210        220        230        240 
TPFLSNPGTN LVFEDEITAL QPEVDKLKTL NVNKIIALGH SGFEVDKLIA QKVKGVDVVV 

       250        260        270        280        290        300 
GGHSNTFLYT GNPPSKEVPA GQYPFIVTSD DGRKVPVVQA YAFGKYLGYL KVEFDEKGNV 

       310        320        330        340        350        360 
VTSHGNPILL NSSIPEDPNI KADINKWRVK LDNYSTQELG KTIVYLDGTA QSCRFRECNM 

       370        380        390        400        410        420 
GNLICDAMIN NNLRHPDEMS WNHVSMCILN GGGIRSPIDE RNNGTITWEN LAAVLPFGGT 

       430        440        450        460        470        480 
FDLVQLKGST LKKAFEHSVH RYGQATGEFL QVGGIHVVYD ISRNPGDRVV KLEVLCTQCR 

       490        500        510        520        530        540 
VPSYEPLRMD KVYKVILPSF LVSGGDGFQM IKDEKIKHDS GDQDINVVSG YISKMKVLYP 

       550        560        570 
AVEGRIQFSA GSHCCGSFSL IFLSVLAVII ILYQ

« Hide

References

« Hide 'large scale' references
[1]"Purification and cDNA cloning of bovine liver 5'-nucleotidase, a GPI-anchored protein, and its expression in COS cells."
Suzuki K., Furukawa Y., Tamura H., Ejiri N., Suematsu H., Taguchi R., Nakamura S., Suzuki Y., Ikezawa H.
J. Biochem. 113:607-613(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Liver.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Hypothalamus.
[3]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D14541 mRNA. Translation: BAA03408.1.
S64302 mRNA. Translation: AAB27698.1.
BC114093 mRNA. Translation: AAI14094.1.
BT026240 mRNA. Translation: ABG67079.1.
IPIIPI00698673.
PIRJX0269.
RefSeqNP_776554.2. NM_174129.3.
UniGeneBt.57034.

3D structure databases

ProteinModelPortalQ05927.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000015059.

Proteomic databases

PRIDEQ05927.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID281363.
KEGGbta:281363.

Organism-specific databases

CTD4907.

Phylogenomic databases

eggNOGCOG0737.
HOGENOMHOG000247215.
HOVERGENHBG000026.
InParanoidQ05927.
KOK01081.
OrthoDBEOG4TMR1Q.

Enzyme and pathway databases

SABIO-RKQ05927.

Family and domain databases

Gene3D3.90.780.10. 1 hit.
InterProIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Metallo_PEstase_dom.
[Graphical view]
PANTHERPTHR11575. PTHR11575. 1 hit.
PfamPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR01607. APYRASEFAMLY.
SUPFAMSSF55816. 5'-Nucleotdase_C. 1 hit.
PROSITEPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805368.

Entry information

Entry name5NTD_BOVIN
AccessionPrimary (citable) accession number: Q05927
Secondary accession number(s): Q24K05
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 3, 2007
Last modified: April 3, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents