Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q05926

- GLRX8_YEAST

UniProt

Q05926 - GLRX8_YEAST

Protein

Glutaredoxin-8

Gene

GRX8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Glutathione-dependent oxidoreductase with lower activity compared to the other members of the glutaredoxin family. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase.2 Publications

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. glutathione-disulfide reductase activity Source: SGD
    3. protein disulfide oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro

    Keywords - Biological processi

    Electron transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32435-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaredoxin-8
    Alternative name(s):
    Glutathione-dependent oxidoreductase 8
    Gene namesi
    Name:GRX8
    Ordered Locus Names:YLR364W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR364w.
    SGDiS000004356. GRX8.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi25 – 251C → S: Impairs activity. 1 Publication
    Mutagenesisi28 – 281C → S: Impairs activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 109109Glutaredoxin-8PRO_0000268192Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi25 ↔ 28Redox-activeCurated

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiQ05926.
    PaxDbiQ05926.
    PeptideAtlasiQ05926.

    Expressioni

    Inductioni

    Strongly induced by the exposure to arsenic which causes glutathione depletion.1 Publication

    Gene expression databases

    GenevestigatoriQ05926.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi31626. 2 interactions.
    IntActiQ05926. 1 interaction.
    MINTiMINT-4496129.
    STRINGi4932.YLR364W.

    Structurei

    Secondary structure

    1
    109
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1410
    Beta strandi17 – 215
    Helixi28 – 3811
    Turni41 – 433
    Beta strandi44 – 485
    Helixi54 – 6714
    Beta strandi72 – 787
    Beta strandi81 – 844
    Helixi86 – 9510
    Helixi98 – 1047

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2M80NMR-A1-109[»]
    ProteinModelPortaliQ05926.
    SMRiQ05926. Positions 2-98.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 109105GlutaredoxinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutaredoxin family.Curated
    Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0695.
    OMAiRKWVPTI.
    OrthoDBiEOG7JMGSQ.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002109. Glutaredoxin.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00462. Glutaredoxin. 1 hit.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05926-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAFVTKAEE MIKSHPYFQL SASWCPDCVY ANSIWNKLNV QDKVFVFDIG    50
    SLPRNEQEKW RIAFQKVVGS RNLPTIVVNG KFWGTESQLH RFEAKGTLEE 100
    ELTKIGLLP 109
    Length:109
    Mass (Da):12,519
    Last modified:November 1, 1996 - v1
    Checksum:i3353EFB47A61574A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19103 Genomic DNA. Translation: AAB67570.1.
    AY558221 Genomic DNA. Translation: AAS56547.1.
    BK006945 Genomic DNA. Translation: DAA09669.1.
    PIRiS51382.
    RefSeqiNP_013468.3. NM_001182253.3.

    Genome annotation databases

    EnsemblFungiiYLR364W; YLR364W; YLR364W.
    GeneIDi851079.
    KEGGisce:YLR364W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19103 Genomic DNA. Translation: AAB67570.1 .
    AY558221 Genomic DNA. Translation: AAS56547.1 .
    BK006945 Genomic DNA. Translation: DAA09669.1 .
    PIRi S51382.
    RefSeqi NP_013468.3. NM_001182253.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2M80 NMR - A 1-109 [» ]
    ProteinModelPortali Q05926.
    SMRi Q05926. Positions 2-98.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31626. 2 interactions.
    IntActi Q05926. 1 interaction.
    MINTi MINT-4496129.
    STRINGi 4932.YLR364W.

    Proteomic databases

    MaxQBi Q05926.
    PaxDbi Q05926.
    PeptideAtlasi Q05926.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR364W ; YLR364W ; YLR364W .
    GeneIDi 851079.
    KEGGi sce:YLR364W.

    Organism-specific databases

    CYGDi YLR364w.
    SGDi S000004356. GRX8.

    Phylogenomic databases

    eggNOGi COG0695.
    OMAi RKWVPTI.
    OrthoDBi EOG7JMGSQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32435-MONOMER.

    Miscellaneous databases

    NextBioi 967738.

    Gene expression databases

    Genevestigatori Q05926.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002109. Glutaredoxin.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00462. Glutaredoxin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 1 hit.
    PROSITEi PS51354. GLUTAREDOXIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Integrating phenotypic and expression profiles to map arsenic-response networks."
      Haugen A.C., Kelley R., Collins J.B., Tucker C.J., Deng C., Afshari C.A., Brown J.M., Ideker T., Van Houten B.
      Genome Biol. 5:R95.1-R95.18(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "A novel group of glutaredoxins in the cis-Golgi critical for oxidative stress resistance."
      Mesecke N., Spang A., Deponte M., Herrmann J.M.
      Mol. Biol. Cell 19:2673-2680(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Biochemical characterization of dithiol glutaredoxin 8 from Saccharomyces cerevisiae: the catalytic redox mechanism redux."
      Eckers E., Bien M., Stroobant V., Herrmann J.M., Deponte M.
      Biochemistry 48:1410-1423(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF CYS-25 AND CYS-28.

    Entry informationi

    Entry nameiGLRX8_YEAST
    AccessioniPrimary (citable) accession number: Q05926
    Secondary accession number(s): D6VZ03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 573 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3