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Q05926 (GLRX8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaredoxin-8
Alternative name(s):
Glutathione-dependent oxidoreductase 8
Gene names
Name:GRX8
Ordered Locus Names:YLR364W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length109 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glutathione-dependent oxidoreductase with lower activity compared to the other members of the glutaredoxin family. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Ref.7 Ref.8

Subunit structure

Monomer. Ref.8

Subcellular location

Cytoplasm Ref.4.

Induction

Strongly induced by the exposure to arsenic which causes glutathione depletion. Ref.6

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glutaredoxin family.

Contains 1 glutaredoxin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 109109Glutaredoxin-8
PRO_0000268192

Regions

Domain5 – 109105Glutaredoxin

Amino acid modifications

Disulfide bond25 ↔ 28Redox-active Probable

Experimental info

Mutagenesis251C → S: Impairs activity. Ref.8
Mutagenesis281C → S: Impairs activity. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q05926 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3353EFB47A61574A

FASTA10912,519
        10         20         30         40         50         60 
MSAFVTKAEE MIKSHPYFQL SASWCPDCVY ANSIWNKLNV QDKVFVFDIG SLPRNEQEKW 

        70         80         90        100 
RIAFQKVVGS RNLPTIVVNG KFWGTESQLH RFEAKGTLEE ELTKIGLLP 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Integrating phenotypic and expression profiles to map arsenic-response networks."
Haugen A.C., Kelley R., Collins J.B., Tucker C.J., Deng C., Afshari C.A., Brown J.M., Ideker T., Van Houten B.
Genome Biol. 5:R95.1-R95.18(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"A novel group of glutaredoxins in the cis-Golgi critical for oxidative stress resistance."
Mesecke N., Spang A., Deponte M., Herrmann J.M.
Mol. Biol. Cell 19:2673-2680(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Biochemical characterization of dithiol glutaredoxin 8 from Saccharomyces cerevisiae: the catalytic redox mechanism redux."
Eckers E., Bien M., Stroobant V., Herrmann J.M., Deponte M.
Biochemistry 48:1410-1423(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF CYS-25 AND CYS-28.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U19103 Genomic DNA. Translation: AAB67570.1.
AY558221 Genomic DNA. Translation: AAS56547.1.
BK006945 Genomic DNA. Translation: DAA09669.1.
PIRS51382.
RefSeqNP_013468.3. NM_001182253.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2M80NMR-A1-109[»]
ProteinModelPortalQ05926.
SMRQ05926. Positions 2-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31626. 2 interactions.
IntActQ05926. 1 interaction.
MINTMINT-4496129.
STRING4932.YLR364W.

Proteomic databases

MaxQBQ05926.
PaxDbQ05926.
PeptideAtlasQ05926.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR364W; YLR364W; YLR364W.
GeneID851079.
KEGGsce:YLR364W.

Organism-specific databases

CYGDYLR364w.
SGDS000004356. GRX8.

Phylogenomic databases

eggNOGCOG0695.
OMARKWVPTI.
OrthoDBEOG7JMGSQ.

Enzyme and pathway databases

BioCycYEAST:G3O-32435-MONOMER.

Gene expression databases

GenevestigatorQ05926.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR002109. Glutaredoxin.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMSSF52833. SSF52833. 1 hit.
PROSITEPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967738.

Entry information

Entry nameGLRX8_YEAST
AccessionPrimary (citable) accession number: Q05926
Secondary accession number(s): D6VZ03
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references