Q05925 (HME1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein engrailed-1
Short name=Homeobox protein en-1
Short name=Hu-En-1

Gene names

Name:

EN1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus.
Sequence similarities	Belongs to the engrailed homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anatomical structure morphogenesis Traceable author statement PubMed 8094370. Source: ProtInc dorsal/ventral pattern formation Inferred from electronic annotation. Source: Compara embryonic forelimb morphogenesis Inferred from electronic annotation. Source: Compara hindbrain development Inferred from electronic annotation. Source: Compara midbrain development Inferred from electronic annotation. Source: Compara midbrain-hindbrain boundary development Inferred from electronic annotation. Source: Compara neuron development Inferred from electronic annotation. Source: Compara pigmentation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara proximal/distal pattern formation Inferred from electronic annotation. Source: Compara skeletal system development Traceable author statement PubMed 8684466. Source: ProtInc
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Homeobox protein engrailed-1		PRO_0000196062
Regions
	DNA binding	303 – 362	60	Homeobox
	Compositional bias	199 – 218	20	Poly-Ala
	Compositional bias	224 – 231	8	Poly-Gly
Experimental info
	Sequence conflict	18	1	A → G in AAA53502. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05925 [UniParc]. Last modified November 25, 2008. Version 3. Checksum: BDFE6279E9C27BFD	FASTA	392	40,115
	10 20 30 40 50 60 MEEQQPEPKS QRDSALGAAA AATPGGLSLS LSPGASGSSG SGSDGDSVPV SPQPAPPSPP 70 80 90 100 110 120 AAPCLPPLAH HPHLPPHPPP PPPQHLAAPA HQPQPAAQLH RTTNFFIDNI LRPDFGCKKE 130 140 150 160 170 180 QPPPQLLVAA AARGGAGGGG RVERDRGQTA AGRDPVHPLG TRAPGAASLL CAPDANCGPP 190 200 210 220 230 240 DGSQPAAAGA GASKAGNPAA AAAAAAAAVA AAAAAAAAKP SDTGGGGSGG GAGSPGAQGT 250 260 270 280 290 300 KYPEHGNPAI LLMGSANGGP VVKTDSQQPL VWPAWVYCTR YSDRPSSGPR TRKLKKKKNE 310 320 330 340 350 360 KEDKRPRTAF TAEQLQRLKA EFQANRYITE QRRQTLAQEL SLNESQIKIW FQNKRAKIKK 370 380 390 ATGIKNGLAL HLMAQGLYNH STTTVQDKDE SE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions." Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J., Joyner A.L. Dev. Genet. 13:345-358(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J., Joyner A.L. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 288.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12699, L12698 Genomic DNA. Translation: AAA53502.2. AC012665 Genomic DNA. Translation: AAX88967.1. CH471103 Genomic DNA. Translation: EAW95206.1.
IPI	IPI00016734.
PIR	B48423.
RefSeq	NP_001417.3. NM_001426.3.
UniGene	Hs.271977.
3D structure databases
ProteinModelPortal	Q05925.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000295206.
PTM databases
PhosphoSite	Q05925.
Polymorphism databases
DMDM	215274149.
Proteomic databases
PaxDb	Q05925.
PRIDE	Q05925.
Protocols and materials databases
DNASU	2019.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295206; ENSP00000295206; ENSG00000163064.
GeneID	2019.
KEGG	hsa:2019.
UCSC	uc002tlm.3. human.
Organism-specific databases
CTD	2019.
GeneCards	GC02M119694.
HGNC	HGNC:3342. EN1.
HPA	CAB014884.
MIM	131290. gene.
neXtProt	NX_Q05925.
PharmGKB	PA27779.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG238866.
HOGENOM	HOG000247054.
HOVERGEN	HBG005975.
InParanoid	Q05925.
KO	K09319.
OMA	NCGPPDG.
OrthoDB	EOG4H72D0.
PhylomeDB	Q05925.
Gene expression databases
Bgee	Q05925.
CleanEx	HS_EN1.
Genevestigator	Q05925.
GermOnline	ENSG00000163064. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR019549. Homeobox-engrailed_C-terminal. IPR017970. Homeobox_CS. IPR020479. Homeobox_metazoa. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR000747. Homeodomain_engrailed. IPR019737. Homoebox-engrailed_CS. [Graphical view]
Pfam	PF10525. Engrail_1_C_sig. 1 hit. PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00026. ENGRAILED. PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00033. ENGRAILED. 1 hit. PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	2019.
NextBio	8181.
SOURCE	Search...

Entry information

Entry name

HME1_HUMAN

Accession

Primary (citable) accession number: Q05925
Secondary accession number(s): Q4ZG44

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.