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Protein

Homeobox protein engrailed-1

Gene

EN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi303 – 36260HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB

GO - Biological processi

  1. anatomical structure morphogenesis Source: ProtInc
  2. dorsal/ventral pattern formation Source: Ensembl
  3. embryonic forelimb morphogenesis Source: Ensembl
  4. hindbrain development Source: Ensembl
  5. midbrain development Source: Ensembl
  6. midbrain-hindbrain boundary development Source: Ensembl
  7. multicellular organism growth Source: Ensembl
  8. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  9. neuron development Source: Ensembl
  10. pigmentation Source: Ensembl
  11. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  12. proximal/distal pattern formation Source: Ensembl
  13. response to cocaine Source: Ensembl
  14. skeletal system development Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ05925.

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein engrailed-1
Short name:
Homeobox protein en-1
Short name:
Hu-En-1
Gene namesi
Name:EN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:3342. EN1.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27779.

Polymorphism and mutation databases

BioMutaiEN1.
DMDMi215274149.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Homeobox protein engrailed-1PRO_0000196062Add
BLAST

Proteomic databases

MaxQBiQ05925.
PaxDbiQ05925.
PRIDEiQ05925.

PTM databases

PhosphoSiteiQ05925.

Expressioni

Gene expression databases

BgeeiQ05925.
CleanExiHS_EN1.
GenevestigatoriQ05925.

Organism-specific databases

HPAiCAB014884.

Interactioni

Protein-protein interaction databases

BioGridi108334. 3 interactions.
STRINGi9606.ENSP00000295206.

Structurei

3D structure databases

ProteinModelPortaliQ05925.
SMRiQ05925. Positions 282-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi199 – 21820Poly-AlaAdd
BLAST
Compositional biasi224 – 2318Poly-Gly

Sequence similaritiesi

Belongs to the engrailed homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG238866.
GeneTreeiENSGT00780000121984.
HOGENOMiHOG000247054.
HOVERGENiHBG005975.
InParanoidiQ05925.
KOiK09319.
OMAiNCGPPDG.
OrthoDBiEOG7VTDN8.
PhylomeDBiQ05925.
TreeFamiTF106461.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR019549. Homeobox-engrailed_C-terminal.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000747. Homeodomain_engrailed.
IPR019737. Homoebox-engrailed_CS.
[Graphical view]
PfamiPF10525. Engrail_1_C_sig. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00026. ENGRAILED.
PR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00033. ENGRAILED. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEQQPEPKS QRDSALGAAA AATPGGLSLS LSPGASGSSG SGSDGDSVPV
60 70 80 90 100
SPQPAPPSPP AAPCLPPLAH HPHLPPHPPP PPPQHLAAPA HQPQPAAQLH
110 120 130 140 150
RTTNFFIDNI LRPDFGCKKE QPPPQLLVAA AARGGAGGGG RVERDRGQTA
160 170 180 190 200
AGRDPVHPLG TRAPGAASLL CAPDANCGPP DGSQPAAAGA GASKAGNPAA
210 220 230 240 250
AAAAAAAAVA AAAAAAAAKP SDTGGGGSGG GAGSPGAQGT KYPEHGNPAI
260 270 280 290 300
LLMGSANGGP VVKTDSQQPL VWPAWVYCTR YSDRPSSGPR TRKLKKKKNE
310 320 330 340 350
KEDKRPRTAF TAEQLQRLKA EFQANRYITE QRRQTLAQEL SLNESQIKIW
360 370 380 390
FQNKRAKIKK ATGIKNGLAL HLMAQGLYNH STTTVQDKDE SE
Length:392
Mass (Da):40,115
Last modified:November 25, 2008 - v3
Checksum:iBDFE6279E9C27BFD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181A → G in AAA53502 (PubMed:1363401).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12699, L12698 Genomic DNA. Translation: AAA53502.2.
AC012665 Genomic DNA. Translation: AAX88967.1.
CH471103 Genomic DNA. Translation: EAW95206.1.
CCDSiCCDS2123.1.
PIRiB48423.
RefSeqiNP_001417.3. NM_001426.3.
UniGeneiHs.271977.

Genome annotation databases

EnsembliENST00000295206; ENSP00000295206; ENSG00000163064.
GeneIDi2019.
KEGGihsa:2019.
UCSCiuc002tlm.3. human.

Polymorphism and mutation databases

BioMutaiEN1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12699, L12698 Genomic DNA. Translation: AAA53502.2.
AC012665 Genomic DNA. Translation: AAX88967.1.
CH471103 Genomic DNA. Translation: EAW95206.1.
CCDSiCCDS2123.1.
PIRiB48423.
RefSeqiNP_001417.3. NM_001426.3.
UniGeneiHs.271977.

3D structure databases

ProteinModelPortaliQ05925.
SMRiQ05925. Positions 282-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108334. 3 interactions.
STRINGi9606.ENSP00000295206.

PTM databases

PhosphoSiteiQ05925.

Polymorphism and mutation databases

BioMutaiEN1.
DMDMi215274149.

Proteomic databases

MaxQBiQ05925.
PaxDbiQ05925.
PRIDEiQ05925.

Protocols and materials databases

DNASUi2019.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295206; ENSP00000295206; ENSG00000163064.
GeneIDi2019.
KEGGihsa:2019.
UCSCiuc002tlm.3. human.

Organism-specific databases

CTDi2019.
GeneCardsiGC02M119694.
HGNCiHGNC:3342. EN1.
HPAiCAB014884.
MIMi131290. gene.
neXtProtiNX_Q05925.
PharmGKBiPA27779.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG238866.
GeneTreeiENSGT00780000121984.
HOGENOMiHOG000247054.
HOVERGENiHBG005975.
InParanoidiQ05925.
KOiK09319.
OMAiNCGPPDG.
OrthoDBiEOG7VTDN8.
PhylomeDBiQ05925.
TreeFamiTF106461.

Enzyme and pathway databases

SignaLinkiQ05925.

Miscellaneous databases

GeneWikiiEN1_(gene).
GenomeRNAii2019.
NextBioi8181.
PROiQ05925.
SOURCEiSearch...

Gene expression databases

BgeeiQ05925.
CleanExiHS_EN1.
GenevestigatoriQ05925.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR019549. Homeobox-engrailed_C-terminal.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000747. Homeodomain_engrailed.
IPR019737. Homoebox-engrailed_CS.
[Graphical view]
PfamiPF10525. Engrail_1_C_sig. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00026. ENGRAILED.
PR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00033. ENGRAILED. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions."
    Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J., Joyner A.L.
    Dev. Genet. 13:345-358(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J., Joyner A.L.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 288.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiHME1_HUMAN
AccessioniPrimary (citable) accession number: Q05925
Secondary accession number(s): Q4ZG44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 25, 2008
Last modified: April 29, 2015
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.