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Q05922 (DUS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein phosphatase 2

EC=3.1.3.16
EC=3.1.3.48
Alternative name(s):
Dual specificity protein phosphatase PAC-1
Gene names
Name:Dusp2
Synonyms:Pac-1, Pac1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subcellular location

Nucleus.

Tissue specificity

In hematopoietic tissues such as spleen and thymus.

Induction

By mitogens.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Contains 1 rhodanese domain.

Contains 1 tyrosine-protein phosphatase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MAPK1P284822EBI-7898692,EBI-959949From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q05922-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q05922-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     175-179: GGPVE → VSSDL
     180-318: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Dual specificity protein phosphatase 2
PRO_0000094794

Regions

Domain27 – 148122Rhodanese
Domain241 – 30666Tyrosine-protein phosphatase

Sites

Active site2611Phosphocysteine intermediate By similarity

Natural variations

Alternative sequence175 – 1795GGPVE → VSSDL in isoform 2.
VSP_005135
Alternative sequence180 – 318139Missing in isoform 2.
VSP_005136

Experimental info

Sequence conflict11 – 122CE → WQ in AAA85136. Ref.2
Sequence conflict201A → V in AAA85136. Ref.2
Sequence conflict641R → P in AAA19666. Ref.1
Sequence conflict641R → P in AAA85136. Ref.2
Sequence conflict1031A → T in AAA19666. Ref.1
Sequence conflict1031A → T in AAA85136. Ref.2
Sequence conflict1561P → A in AAA85136. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: AAB6A01BA598C5C9

FASTA31834,576
        10         20         30         40         50         60 
MPIAMGLETA CELECAALGA LLREPREAER TLLLDCRPFL AFCRSHVRAA RPVPWNALLR 

        70         80         90        100        110        120 
RRARGTPAAA LACLLPDRAL RARLGRGELA RAVVLDESSA SVAELPPDGP AHLLLAALQH 

       130        140        150        160        170        180 
EMRGGPTTVC FLRGGFKSFQ TYCPDLCSEA PAQALPPAGA ENSNSDPRVP IYDQGGPVEI 

       190        200        210        220        230        240 
LPYLYLGSCN HSSDLQGLQA CGITAVLNVS ASCPNHFEGL FHYKSIPVED NQMVEISAWF 

       250        260        270        280        290        300 
QEAISFIDSV KNSGGRVLVH CQAGISRSAT ICLAYLIQSH RVRLDEAFDF VKQRRGVISP 

       310 
NFSFMGQLLQ LETQVLCH 

« Hide

Isoform 2 (Short) [UniParc].

Checksum: E10BE72F8835521A
Show »

FASTA17919,209

References

« Hide 'large scale' references
[1]"PAC-1: a mitogen-induced nuclear protein tyrosine phosphatase."
Rohan P., Davis P., Moskaluk C.A., Kearns M., Krutzsch H., Siebenlist U., Kelly K.
Science 259:1763-1766(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Structure of the gene encoding the murine dual specificity tyrosine-threonine phosphatase PAC1."
Gerondakis S., Economou C., Grumont R.J.
Genomics 24:182-184(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
Strain: 129.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Thymus.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Limb.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11330 mRNA. Translation: AAA19666.1.
U09268 Genomic DNA. Translation: AAA85136.1.
AK134067 mRNA. Translation: BAE21999.1.
AL845368 Genomic DNA. Translation: CAM17446.1.
BC048696 mRNA. Translation: AAH48696.1.
CCDSCCDS16699.1. [Q05922-1]
PIRB57126.
RefSeqNP_034220.2. NM_010090.2. [Q05922-1]
UniGeneMm.4729.

3D structure databases

ProteinModelPortalQ05922.
SMRQ05922. Positions 29-147, 174-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199340. 3 interactions.
IntActQ05922. 2 interactions.
MINTMINT-8214532.

PTM databases

PhosphoSiteQ05922.

Proteomic databases

PRIDEQ05922.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028846; ENSMUSP00000028846; ENSMUSG00000027368. [Q05922-1]
GeneID13537.
KEGGmmu:13537.
UCSCuc008mff.1. mouse. [Q05922-1]

Organism-specific databases

CTD1844.
MGIMGI:101911. Dusp2.

Phylogenomic databases

eggNOGCOG2453.
GeneTreeENSGT00700000104321.
HOGENOMHOG000294080.
HOVERGENHBG007347.
InParanoidQ80ZN1.
KOK04459.
OMACLAYLMQ.
OrthoDBEOG75MVWD.
TreeFamTF105122.

Gene expression databases

BgeeQ05922.
CleanExMM_DUSP2.
GenevestigatorQ05922.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProIPR020417. Atypical_DUSP.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERPTHR10159. PTHR10159. 1 hit.
PfamPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PIRSFPIRSF000939. MAPK_Ptase. 1 hit.
PRINTSPR01908. ADSPHPHTASE.
PR01764. MAPKPHPHTASE.
SMARTSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDUSP2. mouse.
NextBio284136.
PROQ05922.
SOURCESearch...

Entry information

Entry nameDUS2_MOUSE
AccessionPrimary (citable) accession number: Q05922
Secondary accession number(s): Q60640, Q80ZN1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot