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Q05921

- RN5A_MOUSE

UniProt

Q05921 - RN5A_MOUSE

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Protein

2-5A-dependent ribonuclease

Gene

Rnasel

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA turnover (By similarity).By similarity

Catalytic activityi

Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.

Cofactori

Manganese or magnesium. Required for optimal RNA cleavage rates.

Enzyme regulationi

After binding to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) the homodimerization and subsequent activation occurs. Inhibited by RNASEL inhibitor ABCE1/RLI, a cytoplasmic member of the ATP-binding cassette (ABC) transporter family (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri401 – 43636C6-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endonuclease activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. protein kinase activity Source: InterPro
  5. ribonuclease activity Source: Ensembl
  6. rRNA binding Source: Ensembl

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. mRNA processing Source: InterPro
  3. negative regulation of viral genome replication Source: Ensembl
  4. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  5. rRNA processing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
2-5A-dependent ribonuclease (EC:3.1.26.-)
Short name:
2-5A-dependent RNase
Alternative name(s):
Ribonuclease 4
Ribonuclease L
Short name:
RNase L
Gene namesi
Name:Rnasel
Synonyms:Rns4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1098272. Rnasel.

Subcellular locationi

Cytoplasm 1 Publication. Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
  2. nuclear matrix Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7357352-5A-dependent ribonucleasePRO_0000067052Add
BLAST

Proteomic databases

MaxQBiQ05921.
PaxDbiQ05921.
PRIDEiQ05921.

PTM databases

PhosphoSiteiQ05921.

Expressioni

Tissue specificityi

Expressed in spleen, thymus, lung, testis, kidney, liver and heart.

Inductioni

By interferons. Virus replication in higher vertebrates is restrained by IFNs that cause cells to transcribe genes encoding antiviral proteins, such as 2'-5' oligoadenylate synthetases (OASs). oligoadenylate synthetase is stimulated by dsRNA to produce 5'-phosphorylated, 2'-5'-linked oligoadenylates (2-5A), whose function is to activate RNASEL.

Gene expression databases

BgeeiQ05921.
ExpressionAtlasiQ05921. baseline and differential.
GenevestigatoriQ05921.

Interactioni

Subunit structurei

Monomer (inactive form) or homodimer. Interacts with ABCE1; this interaction inhibits the RNASEL (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ05921.
SMRiQ05921. Positions 25-717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati24 – 5330ANK 1Add
BLAST
Repeati58 – 8730ANK 2Add
BLAST
Repeati91 – 12030ANK 3Add
BLAST
Repeati124 – 15330ANK 4Add
BLAST
Repeati167 – 19731ANK 5Add
BLAST
Repeati201 – 23434ANK 6Add
BLAST
Repeati238 – 26831ANK 7Add
BLAST
Repeati272 – 30130ANK 8Add
BLAST
Repeati303 – 32826ANK 9Add
BLAST
Domaini364 – 584221Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini587 – 722136KENPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 242142-5A binding (P-loop) 1Add
BLAST
Regioni253 – 275232-5A binding (P-loop) 2Add
BLAST

Domaini

The nine ankyrin repeats also called 2-5A sensor constitute the N-terminus 2-5A binding domain.
The protein kinase domain is predicted to be catalytically inactive. It allows the homodimerization.
The ribonuclease domain is located in the C-terminus. A single active nuclease domain in a dimer is sufficient for ribonuclease activity.

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 9 ANK repeats.PROSITE-ProRule annotation
Contains 1 KEN domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri401 – 43636C6-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00760000118867.
HOGENOMiHOG000276879.
HOVERGENiHBG012673.
InParanoidiQ05921.
KOiK01165.
OMAiDCGDLVM.
OrthoDBiEOG7VDXNN.
PhylomeDBiQ05921.
TreeFamiTF344032.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
[Graphical view]
PfamiPF00023. Ank. 6 hits.
PF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00580. PUG. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 7 hits.
PS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05921 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METPDYNTPQ GGTPSAGSQR TVVEDDSSLI KAVQKGDVVR VQQLLEKGAD
60 70 80 90 100
ANACEDTWGW TPLHNAVQAG RVDIVNLLLS HGADPHRRKK NGATPFIIAG
110 120 130 140 150
IQGDVKLLEI LLSCGADVNE CDENGFTAFM EAAERGNAEA LRFLFAKGAN
160 170 180 190 200
VNLRRQTTKD KRRLKQGGAT ALMSAAEKGH LEVLRILLND MKAEVDARDN
210 220 230 240 250
MGRNALIRTL LNWDCENVEE ITSILIQHGA DVNVRGERGK TPLIAAVERK
260 270 280 290 300
HTGLVQMLLS REGINIDARD NEGKTALLIA VDKQLKEIVQ LLLEKGADKC
310 320 330 340 350
DDLVWIARRN HDYHLVKLLL PYVANPDTDP PAGDWSPHSS RWGTALKSLH
360 370 380 390 400
SMTRPMIGKL KIFIHDDYKI AGTSEGAVYL GIYDNREVAV KVFRENSPRG
410 420 430 440 450
CKEVSCLRDC GDHSNLVAFY GREDDKGCLY VCVSLCEWTL EEFLRLPREE
460 470 480 490 500
PVENGEDKFA HSILLSIFEG VQKLHLHGYS HQDLQPQNIL IDSKKAVRLA
510 520 530 540 550
DFDQSIRWMG ESQMVRRDLE DLGRLVLYVV MKGEIPFETL KTQNDEVLLT
560 570 580 590 600
MSPDEETKDL IHCLFSPGEN VKNCLVDLLG HPFFWTWENR YRTLRNVGNE
610 620 630 640 650
SDIKVRKCKS DLLRLLQHQT LEPPRSFDQW TSKIDKNVMD EMNHFYEKRK
660 670 680 690 700
KNPYQDTVGD LLKFIRNIGE HINEEKKRGM KEILGDPSRY FQETFPDLVI
710 720 730
YIYKKLKETE YRKHFPQPPP RLSVPEAVGP GGIQS
Length:735
Mass (Da):83,275
Last modified:February 11, 2002 - v2
Checksum:iB6632F4A5B50F711
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF281045 mRNA. Translation: AAG33708.1.
L10382 Genomic DNA. Translation: AAA37117.1.
CCDSiCCDS15377.1.
PIRiB45771.
RefSeqiNP_036012.1. NM_011882.2.
XP_006529588.1. XM_006529525.1.
XP_006529589.1. XM_006529526.1.
XP_006529590.1. XM_006529527.1.
XP_006529591.1. XM_006529528.1.
XP_006529592.1. XM_006529529.1.
XP_006529593.1. XM_006529530.1.
UniGeneiMm.259254.

Genome annotation databases

EnsembliENSMUST00000086209; ENSMUSP00000083385; ENSMUSG00000066800.
GeneIDi24014.
KEGGimmu:24014.
UCSCiuc007daf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF281045 mRNA. Translation: AAG33708.1 .
L10382 Genomic DNA. Translation: AAA37117.1 .
CCDSi CCDS15377.1.
PIRi B45771.
RefSeqi NP_036012.1. NM_011882.2.
XP_006529588.1. XM_006529525.1.
XP_006529589.1. XM_006529526.1.
XP_006529590.1. XM_006529527.1.
XP_006529591.1. XM_006529528.1.
XP_006529592.1. XM_006529529.1.
XP_006529593.1. XM_006529530.1.
UniGenei Mm.259254.

3D structure databases

ProteinModelPortali Q05921.
SMRi Q05921. Positions 25-717.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q05921.
ChEMBLi CHEMBL2687.

PTM databases

PhosphoSitei Q05921.

Proteomic databases

MaxQBi Q05921.
PaxDbi Q05921.
PRIDEi Q05921.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000086209 ; ENSMUSP00000083385 ; ENSMUSG00000066800 .
GeneIDi 24014.
KEGGi mmu:24014.
UCSCi uc007daf.1. mouse.

Organism-specific databases

CTDi 6041.
MGIi MGI:1098272. Rnasel.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00760000118867.
HOGENOMi HOG000276879.
HOVERGENi HBG012673.
InParanoidi Q05921.
KOi K01165.
OMAi DCGDLVM.
OrthoDBi EOG7VDXNN.
PhylomeDBi Q05921.
TreeFami TF344032.

Miscellaneous databases

NextBioi 303947.
PROi Q05921.
SOURCEi Search...

Gene expression databases

Bgeei Q05921.
ExpressionAtlasi Q05921. baseline and differential.
Genevestigatori Q05921.

Family and domain databases

Gene3Di 1.25.40.20. 3 hits.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
[Graphical view ]
Pfami PF00023. Ank. 6 hits.
PF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view ]
PRINTSi PR01415. ANKYRIN.
SMARTi SM00248. ANK. 8 hits.
SM00580. PUG. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 7 hits.
PS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis and origins of the human and mouse RNase L genes: mediators of interferon action."
    Zhou A., Nie H., Silverman R.H.
    Mamm. Genome 11:989-992(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/An.
    Tissue: Adipose tissue.
  2. "Expression cloning of 2-5A-dependent RNAase: a uniquely regulated mediator of interferon action."
    Zhou A., Hassel B.A., Silverman R.H.
    Cell 72:753-765(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-679.
  3. "The 2-5A/RNase L/RNase L inhibitor (RNI) pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells."
    Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.
    J. Biol. Chem. 276:48473-48482(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Erratum
    Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.
    J. Biol. Chem. 277:13354-13354(2002)

Entry informationi

Entry nameiRN5A_MOUSE
AccessioniPrimary (citable) accession number: Q05921
Secondary accession number(s): Q9ERU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 11, 2002
Last modified: October 29, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3