SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q05921

- RN5A_MOUSE

UniProt

Q05921 - RN5A_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
2-5A-dependent ribonuclease
Gene
Rnasel, Rns4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA turnover By similarity.1 Publication

Catalytic activityi

Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.

Cofactori

Manganese or magnesium. Required for optimal RNA cleavage rates.

Enzyme regulationi

After binding to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) the homodimerization and subsequent activation occurs. Inhibited by RNASEL inhibitor ABCE1/RLI, a cytoplasmic member of the ATP-binding cassette (ABC) transporter family By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri401 – 43636C6-type Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endonuclease activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. protein kinase activity Source: InterPro
  5. rRNA binding Source: Ensembl
  6. ribonuclease activity Source: Ensembl

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. mRNA processing Source: InterPro
  3. negative regulation of viral genome replication Source: Ensembl
  4. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  5. rRNA processing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
2-5A-dependent ribonuclease (EC:3.1.26.-)
Short name:
2-5A-dependent RNase
Alternative name(s):
Ribonuclease 4
Ribonuclease L
Short name:
RNase L
Gene namesi
Name:Rnasel
Synonyms:Rns4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1098272. Rnasel.

Subcellular locationi

Cytoplasm. Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. nuclear matrix Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7357352-5A-dependent ribonuclease
PRO_0000067052Add
BLAST

Proteomic databases

MaxQBiQ05921.
PaxDbiQ05921.
PRIDEiQ05921.

PTM databases

PhosphoSiteiQ05921.

Expressioni

Tissue specificityi

Expressed in spleen, thymus, lung, testis, kidney, liver and heart.

Inductioni

By interferons. Virus replication in higher vertebrates is restrained by IFNs that cause cells to transcribe genes encoding antiviral proteins, such as 2'-5' oligoadenylate synthetases (OASs). oligoadenylate synthetase is stimulated by dsRNA to produce 5'-phosphorylated, 2'-5'-linked oligoadenylates (2-5A), whose function is to activate RNASEL.

Gene expression databases

ArrayExpressiQ05921.
BgeeiQ05921.
GenevestigatoriQ05921.

Interactioni

Subunit structurei

Monomer (inactive form) or homodimer. Interacts with ABCE1; this interaction inhibits the RNASEL By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ05921.
SMRiQ05921. Positions 25-717.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati24 – 5330ANK 1
Add
BLAST
Repeati58 – 8730ANK 2
Add
BLAST
Repeati91 – 12030ANK 3
Add
BLAST
Repeati124 – 15330ANK 4
Add
BLAST
Repeati167 – 19731ANK 5
Add
BLAST
Repeati201 – 23434ANK 6
Add
BLAST
Repeati238 – 26831ANK 7
Add
BLAST
Repeati272 – 30130ANK 8
Add
BLAST
Repeati303 – 32826ANK 9
Add
BLAST
Domaini364 – 584221Protein kinase
Add
BLAST
Domaini587 – 722136KEN
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 242142-5A binding (P-loop) 1
Add
BLAST
Regioni253 – 275232-5A binding (P-loop) 2
Add
BLAST

Domaini

The nine ankyrin repeats also called 2-5A sensor constitute the N-terminus 2-5A binding domain.
The protein kinase domain is predicted to be catalytically inactive. It allows the homodimerization.
The ribonuclease domain is located in the C-terminus. A single active nuclease domain in a dimer is sufficient for ribonuclease activity.

Sequence similaritiesi

Contains 9 ANK repeats.
Contains 1 KEN domain.

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00750000117792.
HOGENOMiHOG000276879.
HOVERGENiHBG012673.
InParanoidiQ05921.
KOiK01165.
OMAiDCGDLVM.
OrthoDBiEOG7VDXNN.
PhylomeDBiQ05921.
TreeFamiTF344032.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
[Graphical view]
PfamiPF00023. Ank. 6 hits.
PF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00580. PUG. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 7 hits.
PS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05921-1 [UniParc]FASTAAdd to Basket

« Hide

METPDYNTPQ GGTPSAGSQR TVVEDDSSLI KAVQKGDVVR VQQLLEKGAD    50
ANACEDTWGW TPLHNAVQAG RVDIVNLLLS HGADPHRRKK NGATPFIIAG 100
IQGDVKLLEI LLSCGADVNE CDENGFTAFM EAAERGNAEA LRFLFAKGAN 150
VNLRRQTTKD KRRLKQGGAT ALMSAAEKGH LEVLRILLND MKAEVDARDN 200
MGRNALIRTL LNWDCENVEE ITSILIQHGA DVNVRGERGK TPLIAAVERK 250
HTGLVQMLLS REGINIDARD NEGKTALLIA VDKQLKEIVQ LLLEKGADKC 300
DDLVWIARRN HDYHLVKLLL PYVANPDTDP PAGDWSPHSS RWGTALKSLH 350
SMTRPMIGKL KIFIHDDYKI AGTSEGAVYL GIYDNREVAV KVFRENSPRG 400
CKEVSCLRDC GDHSNLVAFY GREDDKGCLY VCVSLCEWTL EEFLRLPREE 450
PVENGEDKFA HSILLSIFEG VQKLHLHGYS HQDLQPQNIL IDSKKAVRLA 500
DFDQSIRWMG ESQMVRRDLE DLGRLVLYVV MKGEIPFETL KTQNDEVLLT 550
MSPDEETKDL IHCLFSPGEN VKNCLVDLLG HPFFWTWENR YRTLRNVGNE 600
SDIKVRKCKS DLLRLLQHQT LEPPRSFDQW TSKIDKNVMD EMNHFYEKRK 650
KNPYQDTVGD LLKFIRNIGE HINEEKKRGM KEILGDPSRY FQETFPDLVI 700
YIYKKLKETE YRKHFPQPPP RLSVPEAVGP GGIQS 735
Length:735
Mass (Da):83,275
Last modified:February 11, 2002 - v2
Checksum:iB6632F4A5B50F711
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF281045 mRNA. Translation: AAG33708.1.
L10382 Genomic DNA. Translation: AAA37117.1.
CCDSiCCDS15377.1.
PIRiB45771.
RefSeqiNP_036012.1. NM_011882.2.
XP_006529588.1. XM_006529525.1.
XP_006529589.1. XM_006529526.1.
XP_006529590.1. XM_006529527.1.
XP_006529591.1. XM_006529528.1.
XP_006529592.1. XM_006529529.1.
XP_006529593.1. XM_006529530.1.
UniGeneiMm.259254.

Genome annotation databases

EnsembliENSMUST00000086209; ENSMUSP00000083385; ENSMUSG00000066800.
ENSMUST00000182538; ENSMUSP00000138734; ENSMUSG00000066800.
GeneIDi24014.
KEGGimmu:24014.
UCSCiuc007daf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF281045 mRNA. Translation: AAG33708.1 .
L10382 Genomic DNA. Translation: AAA37117.1 .
CCDSi CCDS15377.1.
PIRi B45771.
RefSeqi NP_036012.1. NM_011882.2.
XP_006529588.1. XM_006529525.1.
XP_006529589.1. XM_006529526.1.
XP_006529590.1. XM_006529527.1.
XP_006529591.1. XM_006529528.1.
XP_006529592.1. XM_006529529.1.
XP_006529593.1. XM_006529530.1.
UniGenei Mm.259254.

3D structure databases

ProteinModelPortali Q05921.
SMRi Q05921. Positions 25-717.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q05921.
ChEMBLi CHEMBL2687.

PTM databases

PhosphoSitei Q05921.

Proteomic databases

MaxQBi Q05921.
PaxDbi Q05921.
PRIDEi Q05921.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000086209 ; ENSMUSP00000083385 ; ENSMUSG00000066800 .
ENSMUST00000182538 ; ENSMUSP00000138734 ; ENSMUSG00000066800 .
GeneIDi 24014.
KEGGi mmu:24014.
UCSCi uc007daf.1. mouse.

Organism-specific databases

CTDi 6041.
MGIi MGI:1098272. Rnasel.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00750000117792.
HOGENOMi HOG000276879.
HOVERGENi HBG012673.
InParanoidi Q05921.
KOi K01165.
OMAi DCGDLVM.
OrthoDBi EOG7VDXNN.
PhylomeDBi Q05921.
TreeFami TF344032.

Miscellaneous databases

NextBioi 303947.
PROi Q05921.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q05921.
Bgeei Q05921.
Genevestigatori Q05921.

Family and domain databases

Gene3Di 1.25.40.20. 3 hits.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
[Graphical view ]
Pfami PF00023. Ank. 6 hits.
PF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view ]
PRINTSi PR01415. ANKYRIN.
SMARTi SM00248. ANK. 8 hits.
SM00580. PUG. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 7 hits.
PS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis and origins of the human and mouse RNase L genes: mediators of interferon action."
    Zhou A., Nie H., Silverman R.H.
    Mamm. Genome 11:989-992(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/An.
    Tissue: Adipose tissue.
  2. "Expression cloning of 2-5A-dependent RNAase: a uniquely regulated mediator of interferon action."
    Zhou A., Hassel B.A., Silverman R.H.
    Cell 72:753-765(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-679.
  3. "The 2-5A/RNase L/RNase L inhibitor (RNI) pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells."
    Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.
    J. Biol. Chem. 276:48473-48482(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Erratum
    Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.
    J. Biol. Chem. 277:13354-13354(2002)

Entry informationi

Entry nameiRN5A_MOUSE
AccessioniPrimary (citable) accession number: Q05921
Secondary accession number(s): Q9ERU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 11, 2002
Last modified: July 9, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3