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Protein

GTP cyclohydrolase 1

Gene

Gch1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence (By similarity).By similarity

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Enzyme regulationi

GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity. Zinc is required for catalytic activity. Inhibited by Mg2+.

Pathway: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (Gch1)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei87 – 871Involved in pterin ring bindingBy similarity
Sitei96 – 961Involved in pterin ring bindingBy similarity
Metal bindingi132 – 1321ZincBy similarity
Metal bindingi135 – 1351ZincBy similarity
Metal bindingi203 – 2031ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.16. 3474.
ReactomeiREACT_273079. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:Gch1
Synonyms:Gch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:95675. Gch1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1111By similarityPRO_0000010718Add
BLAST
Chaini12 – 241230GTP cyclohydrolase 1PRO_0000010719Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511Phosphoserine1 Publication
Modified residuei72 – 721PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05915.
PaxDbiQ05915.
PRIDEiQ05915.

PTM databases

PhosphoSiteiQ05915.

Expressioni

Gene expression databases

BgeeiQ05915.
CleanExiMM_GCH1.
ExpressionAtlasiQ05915. baseline and differential.
GenevisibleiQ05915. MM.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers. Interacts with AHSA1 and GCHFR/GFRP (By similarity).By similarity

Protein-protein interaction databases

BioGridi199864. 1 interaction.
STRINGi10090.ENSMUSP00000087405.

Structurei

3D structure databases

ProteinModelPortaliQ05915.
SMRiQ05915. Positions 48-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiCOG0302.
HOGENOMiHOG000221222.
HOVERGENiHBG003136.
InParanoidiQ05915.
KOiK01495.
OMAiVIVVTEC.
OrthoDBiEOG77DJ6T.
PhylomeDBiQ05915.
TreeFamiTF105616.

Family and domain databases

HAMAPiMF_00223. FolE.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPRGVRCT NGFSERELPR PGASPPAEKS RPPEAKGAQP ADAWKAGRHR
60 70 80 90 100
SEEENQVNLP KLAAAYSSIL LSLGEDPQRQ GLLKTPWRAA TAMQYFTKGY
110 120 130 140 150
QETISDVLND AIFDEDHDEM VIVKDIDMFS MCEHHLVPFV GRVHIGYLPN
160 170 180 190 200
KQVLGLSKLA RIVEIYSRRL QVQERLTKQI AVAITEALQP AGVGVVIEAT
210 220 230 240
HMCMVMRGVQ KMNSKTVTST MLGVFREDPK TREEFLTLIR S
Length:241
Mass (Da):27,014
Last modified:February 1, 1994 - v1
Checksum:iB4366F102B4A6FE6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541E → D in AAC60677 (PubMed:8304101).Curated
Sequence conflicti56 – 561Q → E in AAC60677 (PubMed:8304101).Curated
Sequence conflicti235 – 2351F → S in AAC60677 (PubMed:8304101).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09737 mRNA. Translation: AAA37757.1.
BC005643 mRNA. Translation: AAH05643.1.
BC069921 mRNA. Translation: AAH69921.1.
S71373 mRNA. Translation: AAC60677.2.
CCDSiCCDS26984.1.
PIRiJQ1934.
RefSeqiNP_032128.1. NM_008102.3.
UniGeneiMm.10651.

Genome annotation databases

EnsembliENSMUST00000089959; ENSMUSP00000087405; ENSMUSG00000037580.
GeneIDi14528.
KEGGimmu:14528.
UCSCiuc007tht.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09737 mRNA. Translation: AAA37757.1.
BC005643 mRNA. Translation: AAH05643.1.
BC069921 mRNA. Translation: AAH69921.1.
S71373 mRNA. Translation: AAC60677.2.
CCDSiCCDS26984.1.
PIRiJQ1934.
RefSeqiNP_032128.1. NM_008102.3.
UniGeneiMm.10651.

3D structure databases

ProteinModelPortaliQ05915.
SMRiQ05915. Positions 48-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199864. 1 interaction.
STRINGi10090.ENSMUSP00000087405.

PTM databases

PhosphoSiteiQ05915.

Proteomic databases

MaxQBiQ05915.
PaxDbiQ05915.
PRIDEiQ05915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089959; ENSMUSP00000087405; ENSMUSG00000037580.
GeneIDi14528.
KEGGimmu:14528.
UCSCiuc007tht.1. mouse.

Organism-specific databases

CTDi2643.
MGIiMGI:95675. Gch1.

Phylogenomic databases

eggNOGiCOG0302.
HOGENOMiHOG000221222.
HOVERGENiHBG003136.
InParanoidiQ05915.
KOiK01495.
OMAiVIVVTEC.
OrthoDBiEOG77DJ6T.
PhylomeDBiQ05915.
TreeFamiTF105616.

Enzyme and pathway databases

UniPathwayiUPA00848; UER00151.
BRENDAi3.5.4.16. 3474.
ReactomeiREACT_273079. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

NextBioi286176.
PROiQ05915.
SOURCEiSearch...

Gene expression databases

BgeeiQ05915.
CleanExiMM_GCH1.
ExpressionAtlasiQ05915. baseline and differential.
GenevisibleiQ05915. MM.

Family and domain databases

HAMAPiMF_00223. FolE.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Mammary gland.
  3. "Detection and quantification of GTP cyclohydrolase I mRNA."
    Gutlich M., Schott K., Werner T., Bacher A., Ziegler I.
    Adv. Exp. Med. Biol. 338:167-170(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-241.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGCH1_MOUSE
AccessioniPrimary (citable) accession number: Q05915
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 24, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.