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Q05915

- GCH1_MOUSE

UniProt

Q05915 - GCH1_MOUSE

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Protein

GTP cyclohydrolase 1

Gene

Gch1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence (By similarity).By similarity

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Enzyme regulationi

GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity. Zinc is required for catalytic activity. Inhibited by Mg2+.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei87 – 871Involved in pterin ring bindingBy similarity
Sitei96 – 961Involved in pterin ring bindingBy similarity
Metal bindingi132 – 1321ZincBy similarity
Metal bindingi135 – 1351ZincBy similarity
Metal bindingi203 – 2031ZincBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: Ensembl
  2. coenzyme binding Source: Ensembl
  3. GTP binding Source: UniProtKB-KW
  4. GTP cyclohydrolase I activity Source: MGI
  5. zinc ion binding Source: Ensembl

GO - Biological processi

  1. 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. dopamine biosynthetic process Source: Ensembl
  3. GTP catabolic process Source: Ensembl
  4. negative regulation of blood pressure Source: Ensembl
  5. neuromuscular process controlling posture Source: Ensembl
  6. positive regulation of nitric-oxide synthase activity Source: Ensembl
  7. protein heterooligomerization Source: Ensembl
  8. protein homooligomerization Source: Ensembl
  9. regulation of blood pressure Source: MGI
  10. regulation of lung blood pressure Source: MGI
  11. response to interferon-gamma Source: Ensembl
  12. response to lipopolysaccharide Source: Ensembl
  13. response to pain Source: UniProtKB
  14. response to tumor necrosis factor Source: Ensembl
  15. tetrahydrobiopterin biosynthetic process Source: MGI
  16. tetrahydrofolate biosynthetic process Source: InterPro
  17. vasodilation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.16. 3474.
ReactomeiREACT_199047. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:Gch1
Synonyms:Gch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:95675. Gch1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: Ensembl
  2. cytosol Source: Ensembl
  3. nuclear membrane Source: Ensembl
  4. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1111By similarityPRO_0000010718Add
BLAST
Chaini12 – 241230GTP cyclohydrolase 1PRO_0000010719Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511Phosphoserine1 Publication
Modified residuei72 – 721PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05915.
PaxDbiQ05915.
PRIDEiQ05915.

PTM databases

PhosphoSiteiQ05915.

Expressioni

Gene expression databases

BgeeiQ05915.
CleanExiMM_GCH1.
ExpressionAtlasiQ05915. baseline and differential.
GenevestigatoriQ05915.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers. Interacts with AHSA1 and GCHFR/GFRP (By similarity).By similarity

Protein-protein interaction databases

BioGridi199864. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ05915.
SMRiQ05915. Positions 48-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiCOG0302.
HOGENOMiHOG000221222.
HOVERGENiHBG003136.
InParanoidiQ05915.
KOiK01495.
OMAiKRTYDEE.
OrthoDBiEOG77DJ6T.
PhylomeDBiQ05915.
TreeFamiTF105616.

Family and domain databases

HAMAPiMF_00223. FolE.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05915-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKPRGVRCT NGFSERELPR PGASPPAEKS RPPEAKGAQP ADAWKAGRHR
60 70 80 90 100
SEEENQVNLP KLAAAYSSIL LSLGEDPQRQ GLLKTPWRAA TAMQYFTKGY
110 120 130 140 150
QETISDVLND AIFDEDHDEM VIVKDIDMFS MCEHHLVPFV GRVHIGYLPN
160 170 180 190 200
KQVLGLSKLA RIVEIYSRRL QVQERLTKQI AVAITEALQP AGVGVVIEAT
210 220 230 240
HMCMVMRGVQ KMNSKTVTST MLGVFREDPK TREEFLTLIR S
Length:241
Mass (Da):27,014
Last modified:February 1, 1994 - v1
Checksum:iB4366F102B4A6FE6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541E → D in AAC60677. (PubMed:8304101)Curated
Sequence conflicti56 – 561Q → E in AAC60677. (PubMed:8304101)Curated
Sequence conflicti235 – 2351F → S in AAC60677. (PubMed:8304101)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09737 mRNA. Translation: AAA37757.1.
BC005643 mRNA. Translation: AAH05643.1.
BC069921 mRNA. Translation: AAH69921.1.
S71373 mRNA. Translation: AAC60677.2.
CCDSiCCDS26984.1.
PIRiJQ1934.
RefSeqiNP_032128.1. NM_008102.3.
UniGeneiMm.10651.

Genome annotation databases

EnsembliENSMUST00000089959; ENSMUSP00000087405; ENSMUSG00000037580.
GeneIDi14528.
KEGGimmu:14528.
UCSCiuc007tht.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09737 mRNA. Translation: AAA37757.1 .
BC005643 mRNA. Translation: AAH05643.1 .
BC069921 mRNA. Translation: AAH69921.1 .
S71373 mRNA. Translation: AAC60677.2 .
CCDSi CCDS26984.1.
PIRi JQ1934.
RefSeqi NP_032128.1. NM_008102.3.
UniGenei Mm.10651.

3D structure databases

ProteinModelPortali Q05915.
SMRi Q05915. Positions 48-241.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199864. 1 interaction.

PTM databases

PhosphoSitei Q05915.

Proteomic databases

MaxQBi Q05915.
PaxDbi Q05915.
PRIDEi Q05915.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000089959 ; ENSMUSP00000087405 ; ENSMUSG00000037580 .
GeneIDi 14528.
KEGGi mmu:14528.
UCSCi uc007tht.1. mouse.

Organism-specific databases

CTDi 2643.
MGIi MGI:95675. Gch1.

Phylogenomic databases

eggNOGi COG0302.
HOGENOMi HOG000221222.
HOVERGENi HBG003136.
InParanoidi Q05915.
KOi K01495.
OMAi KRTYDEE.
OrthoDBi EOG77DJ6T.
PhylomeDBi Q05915.
TreeFami TF105616.

Enzyme and pathway databases

UniPathwayi UPA00848 ; UER00151 .
BRENDAi 3.5.4.16. 3474.
Reactomei REACT_199047. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

NextBioi 286176.
PROi Q05915.
SOURCEi Search...

Gene expression databases

Bgeei Q05915.
CleanExi MM_GCH1.
ExpressionAtlasi Q05915. baseline and differential.
Genevestigatori Q05915.

Family and domain databases

HAMAPi MF_00223. FolE.
InterProi IPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view ]
PANTHERi PTHR11109. PTHR11109. 1 hit.
Pfami PF01227. GTP_cyclohydroI. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00063. folE. 1 hit.
PROSITEi PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Mammary gland.
  3. "Detection and quantification of GTP cyclohydrolase I mRNA."
    Gutlich M., Schott K., Werner T., Bacher A., Ziegler I.
    Adv. Exp. Med. Biol. 338:167-170(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-241.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGCH1_MOUSE
AccessioniPrimary (citable) accession number: Q05915
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3