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Protein

GTP cyclohydrolase 1

Gene

Gch1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence.By similarity

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.By similarity

Enzyme regulationi

GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity. Zinc is required for catalytic activity. Inhibited by Mg2+.By similarity

Pathwayi: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (Gch1)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87Involved in pterin ring bindingBy similarity1
Sitei96Involved in pterin ring bindingBy similarity1
Metal bindingi132ZincBy similarity1
Metal bindingi135ZincBy similarity1
Metal bindingi203ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.16. 3474.
ReactomeiR-MMU-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16By similarity)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:Gch1
Synonyms:Gch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:95675. Gch1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000107181 – 11By similarityAdd BLAST11
ChainiPRO_000001071912 – 241GTP cyclohydrolase 1Add BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineCombined sources1
Modified residuei72PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ05915.
PeptideAtlasiQ05915.
PRIDEiQ05915.

PTM databases

iPTMnetiQ05915.
PhosphoSitePlusiQ05915.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037580.
CleanExiMM_GCH1.
ExpressionAtlasiQ05915. baseline and differential.
GenevisibleiQ05915. MM.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers. Interacts with AHSA1 and GCHFR/GFRP.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199864. 1 interactor.
STRINGi10090.ENSMUSP00000087405.

Structurei

3D structure databases

ProteinModelPortaliQ05915.
SMRiQ05915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiKOG2698. Eukaryota.
COG0302. LUCA.
GeneTreeiENSGT00390000013481.
HOGENOMiHOG000221222.
HOVERGENiHBG003136.
InParanoidiQ05915.
KOiK01495.
OMAiMGKVHIG.
OrthoDBiEOG091G0PCE.
PhylomeDBiQ05915.
TreeFamiTF105616.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPRGVRCT NGFSERELPR PGASPPAEKS RPPEAKGAQP ADAWKAGRHR
60 70 80 90 100
SEEENQVNLP KLAAAYSSIL LSLGEDPQRQ GLLKTPWRAA TAMQYFTKGY
110 120 130 140 150
QETISDVLND AIFDEDHDEM VIVKDIDMFS MCEHHLVPFV GRVHIGYLPN
160 170 180 190 200
KQVLGLSKLA RIVEIYSRRL QVQERLTKQI AVAITEALQP AGVGVVIEAT
210 220 230 240
HMCMVMRGVQ KMNSKTVTST MLGVFREDPK TREEFLTLIR S
Length:241
Mass (Da):27,014
Last modified:February 1, 1994 - v1
Checksum:iB4366F102B4A6FE6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54E → D in AAC60677 (PubMed:8304101).Curated1
Sequence conflicti56Q → E in AAC60677 (PubMed:8304101).Curated1
Sequence conflicti235F → S in AAC60677 (PubMed:8304101).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09737 mRNA. Translation: AAA37757.1.
BC005643 mRNA. Translation: AAH05643.1.
BC069921 mRNA. Translation: AAH69921.1.
S71373 mRNA. Translation: AAC60677.2.
CCDSiCCDS26984.1.
PIRiJQ1934.
RefSeqiNP_032128.1. NM_008102.3.
UniGeneiMm.10651.

Genome annotation databases

EnsembliENSMUST00000089959; ENSMUSP00000087405; ENSMUSG00000037580.
GeneIDi14528.
KEGGimmu:14528.
UCSCiuc007tht.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09737 mRNA. Translation: AAA37757.1.
BC005643 mRNA. Translation: AAH05643.1.
BC069921 mRNA. Translation: AAH69921.1.
S71373 mRNA. Translation: AAC60677.2.
CCDSiCCDS26984.1.
PIRiJQ1934.
RefSeqiNP_032128.1. NM_008102.3.
UniGeneiMm.10651.

3D structure databases

ProteinModelPortaliQ05915.
SMRiQ05915.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199864. 1 interactor.
STRINGi10090.ENSMUSP00000087405.

PTM databases

iPTMnetiQ05915.
PhosphoSitePlusiQ05915.

Proteomic databases

PaxDbiQ05915.
PeptideAtlasiQ05915.
PRIDEiQ05915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089959; ENSMUSP00000087405; ENSMUSG00000037580.
GeneIDi14528.
KEGGimmu:14528.
UCSCiuc007tht.1. mouse.

Organism-specific databases

CTDi2643.
MGIiMGI:95675. Gch1.

Phylogenomic databases

eggNOGiKOG2698. Eukaryota.
COG0302. LUCA.
GeneTreeiENSGT00390000013481.
HOGENOMiHOG000221222.
HOVERGENiHBG003136.
InParanoidiQ05915.
KOiK01495.
OMAiMGKVHIG.
OrthoDBiEOG091G0PCE.
PhylomeDBiQ05915.
TreeFamiTF105616.

Enzyme and pathway databases

UniPathwayiUPA00848; UER00151.
BRENDAi3.5.4.16. 3474.
ReactomeiR-MMU-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

PROiQ05915.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037580.
CleanExiMM_GCH1.
ExpressionAtlasiQ05915. baseline and differential.
GenevisibleiQ05915. MM.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCH1_MOUSE
AccessioniPrimary (citable) accession number: Q05915
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.