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Q05915

- GCH1_MOUSE

UniProt

Q05915 - GCH1_MOUSE

Protein

GTP cyclohydrolase 1

Gene

Gch1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence By similarity.By similarity

    Catalytic activityi

    GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

    Enzyme regulationi

    GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity. Zinc is required for catalytic activity. Inhibited by Mg2+.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei87 – 871Involved in pterin ring bindingBy similarity
    Sitei96 – 961Involved in pterin ring bindingBy similarity
    Metal bindingi132 – 1321ZincBy similarity
    Metal bindingi135 – 1351ZincBy similarity
    Metal bindingi203 – 2031ZincBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: Ensembl
    2. coenzyme binding Source: Ensembl
    3. GTP binding Source: UniProtKB-KW
    4. GTP cyclohydrolase I activity Source: MGI
    5. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. dopamine biosynthetic process Source: Ensembl
    3. GTP catabolic process Source: Ensembl
    4. negative regulation of blood pressure Source: Ensembl
    5. neuromuscular process controlling posture Source: Ensembl
    6. positive regulation of nitric-oxide synthase activity Source: Ensembl
    7. protein heterooligomerization Source: Ensembl
    8. protein homooligomerization Source: Ensembl
    9. regulation of blood pressure Source: MGI
    10. regulation of lung blood pressure Source: MGI
    11. response to interferon-gamma Source: Ensembl
    12. response to lipopolysaccharide Source: Ensembl
    13. response to pain Source: UniProtKB
    14. response to tumor necrosis factor Source: Ensembl
    15. tetrahydrobiopterin biosynthetic process Source: MGI
    16. tetrahydrofolate biosynthetic process Source: InterPro
    17. vasodilation Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Tetrahydrobiopterin biosynthesis

    Keywords - Ligandi

    GTP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.5.4.16. 3474.
    ReactomeiREACT_199047. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    UniPathwayiUPA00848; UER00151.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP cyclohydrolase 1 (EC:3.5.4.16)
    Alternative name(s):
    GTP cyclohydrolase I
    Short name:
    GTP-CH-I
    Gene namesi
    Name:Gch1
    Synonyms:Gch
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:95675. Gch1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: Ensembl
    2. cytosol Source: Ensembl
    3. nuclear membrane Source: Ensembl
    4. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 1111By similarityPRO_0000010718Add
    BLAST
    Chaini12 – 241230GTP cyclohydrolase 1PRO_0000010719Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei51 – 511Phosphoserine1 Publication
    Modified residuei72 – 721PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ05915.
    PaxDbiQ05915.
    PRIDEiQ05915.

    PTM databases

    PhosphoSiteiQ05915.

    Expressioni

    Gene expression databases

    BgeeiQ05915.
    CleanExiMM_GCH1.
    GenevestigatoriQ05915.

    Interactioni

    Subunit structurei

    Toroid-shaped homodecamer, composed of two pentamers of five dimers. Interacts with AHSA1 and GCHFR/GFRP By similarity.By similarity

    Protein-protein interaction databases

    BioGridi199864. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05915.
    SMRiQ05915. Positions 48-241.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GTP cyclohydrolase I family.Curated

    Phylogenomic databases

    eggNOGiCOG0302.
    HOGENOMiHOG000221222.
    HOVERGENiHBG003136.
    InParanoidiQ05915.
    KOiK01495.
    OMAiKRTYDEE.
    OrthoDBiEOG77DJ6T.
    PhylomeDBiQ05915.
    TreeFamiTF105616.

    Family and domain databases

    HAMAPiMF_00223. FolE.
    InterProiIPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    [Graphical view]
    PANTHERiPTHR11109. PTHR11109. 1 hit.
    PfamiPF01227. GTP_cyclohydroI. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00063. folE. 1 hit.
    PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05915-1 [UniParc]FASTAAdd to Basket

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    MEKPRGVRCT NGFSERELPR PGASPPAEKS RPPEAKGAQP ADAWKAGRHR    50
    SEEENQVNLP KLAAAYSSIL LSLGEDPQRQ GLLKTPWRAA TAMQYFTKGY 100
    QETISDVLND AIFDEDHDEM VIVKDIDMFS MCEHHLVPFV GRVHIGYLPN 150
    KQVLGLSKLA RIVEIYSRRL QVQERLTKQI AVAITEALQP AGVGVVIEAT 200
    HMCMVMRGVQ KMNSKTVTST MLGVFREDPK TREEFLTLIR S 241
    Length:241
    Mass (Da):27,014
    Last modified:February 1, 1994 - v1
    Checksum:iB4366F102B4A6FE6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 541E → D in AAC60677. (PubMed:8304101)Curated
    Sequence conflicti56 – 561Q → E in AAC60677. (PubMed:8304101)Curated
    Sequence conflicti235 – 2351F → S in AAC60677. (PubMed:8304101)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09737 mRNA. Translation: AAA37757.1.
    BC005643 mRNA. Translation: AAH05643.1.
    BC069921 mRNA. Translation: AAH69921.1.
    S71373 mRNA. Translation: AAC60677.2.
    CCDSiCCDS26984.1.
    PIRiJQ1934.
    RefSeqiNP_032128.1. NM_008102.3.
    UniGeneiMm.10651.

    Genome annotation databases

    EnsembliENSMUST00000089959; ENSMUSP00000087405; ENSMUSG00000037580.
    GeneIDi14528.
    KEGGimmu:14528.
    UCSCiuc007tht.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09737 mRNA. Translation: AAA37757.1 .
    BC005643 mRNA. Translation: AAH05643.1 .
    BC069921 mRNA. Translation: AAH69921.1 .
    S71373 mRNA. Translation: AAC60677.2 .
    CCDSi CCDS26984.1.
    PIRi JQ1934.
    RefSeqi NP_032128.1. NM_008102.3.
    UniGenei Mm.10651.

    3D structure databases

    ProteinModelPortali Q05915.
    SMRi Q05915. Positions 48-241.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199864. 1 interaction.

    PTM databases

    PhosphoSitei Q05915.

    Proteomic databases

    MaxQBi Q05915.
    PaxDbi Q05915.
    PRIDEi Q05915.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000089959 ; ENSMUSP00000087405 ; ENSMUSG00000037580 .
    GeneIDi 14528.
    KEGGi mmu:14528.
    UCSCi uc007tht.1. mouse.

    Organism-specific databases

    CTDi 2643.
    MGIi MGI:95675. Gch1.

    Phylogenomic databases

    eggNOGi COG0302.
    HOGENOMi HOG000221222.
    HOVERGENi HBG003136.
    InParanoidi Q05915.
    KOi K01495.
    OMAi KRTYDEE.
    OrthoDBi EOG77DJ6T.
    PhylomeDBi Q05915.
    TreeFami TF105616.

    Enzyme and pathway databases

    UniPathwayi UPA00848 ; UER00151 .
    BRENDAi 3.5.4.16. 3474.
    Reactomei REACT_199047. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

    Miscellaneous databases

    NextBioi 286176.
    PROi Q05915.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q05915.
    CleanExi MM_GCH1.
    Genevestigatori Q05915.

    Family and domain databases

    HAMAPi MF_00223. FolE.
    InterProi IPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    [Graphical view ]
    PANTHERi PTHR11109. PTHR11109. 1 hit.
    Pfami PF01227. GTP_cyclohydroI. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00063. folE. 1 hit.
    PROSITEi PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Mammary gland.
    3. "Detection and quantification of GTP cyclohydrolase I mRNA."
      Gutlich M., Schott K., Werner T., Bacher A., Ziegler I.
      Adv. Exp. Med. Biol. 338:167-170(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-241.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGCH1_MOUSE
    AccessioniPrimary (citable) accession number: Q05915
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3