Reviewed, UniProtKB/Swiss-Prot Q05913 (T2FA_DROME)

Names and origin

Protein names

Recommended name:
    General transcription factor IIF subunit 1
Alternative name(s):
    Transcription initiation factor IIF subunit alpha
      Short name=TFIIF-alpha
    Transcription factor 5 large chain
      Short name=TF5A

Gene names

Name:	TfIIFalpha
ORF Names:	CG10281

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. Ref.6
Subunit structure	Heterodimer of an alpha and a beta subunit.
Subcellular location	Nucleus.
Post-translational modification	Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases By similarity.
Sequence similarities	Belongs to the TFIIF alpha subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	positive regulation of transcription Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct transcription activator activity Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GTF2F1	P35269	1	EBI-174822,EBI-457886	From a different organism.
NHP2	Q9V3U2	1	EBI-174822,EBI-187697

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	General transcription factor IIF subunit 1		PRO_0000211233
Amino acid modifications
	Modified residue	183	1	Phosphoserine Ref.7
	Modified residue	246	1	Phosphoserine Ref.7
	Modified residue	250	1	Phosphoserine Ref.7
	Modified residue	252	1	Phosphoserine Ref.7
	Modified residue	341	1	Phosphothreonine Ref.7
	Modified residue	342	1	Phosphoserine Ref.7
	Modified residue	352	1	Phosphoserine Ref.7
	Modified residue	355	1	Phosphoserine Ref.7
	Modified residue	453	1	Phosphoserine Ref.7
	Modified residue	455	1	Phosphoserine Ref.7
	Modified residue	457	1	Phosphothreonine Ref.7
	Modified residue	482	1	Phosphoserine Ref.7
	Modified residue	484	1	Phosphoserine Ref.7
	Modified residue	488	1	Phosphothreonine Ref.7
Experimental info
	Sequence conflict	20	1	A → R in L10331. Ref.1
	Sequence conflict	76	1	E → G in L10331. Ref.1
	Sequence conflict	528	1	P → A in L10331. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05913-1 [UniParc]. Last modified December 1, 2000. Version 3. Checksum: 1E3DF88E3C833761	FASTA	577	64,467
	10 20 30 40 50 60 MSSASKSTPS AASGSSTSAA AAAAASVASG SASSSANVQE FKIRVPKMPK KHHVMRFNAT 70 80 90 100 110 120 LNVDFAQWRN VKLERENNMK EFRGMEEDQP KFGAGSEYNR DQREEARRKK FGIIARKYRP 130 140 150 160 170 180 EAQPWILKVG GKTGKKFKGI REGGVGENAA FYVFTHAPDG AIEAYPLTEW YNFQPIQRYK 190 200 210 220 230 240 SLSAEEAEQE FGRRKKVMNY FSLMLRKRLR GDEEEEQDPE EAKLIKAATK KSKELKITDM 250 260 270 280 290 300 DEWIDSEDES DSEDEEDKKK KEQEDSDDGK AKGKGKKGAD KKKKKRDVDD EAFEESDDGD 310 320 330 340 350 360 EEGREMDYDT SSSEDEPDPE AKVDKDMKGV AEEDALRKLL TSDEEEDDEK KSDESDKEDA 370 380 390 400 410 420 DGEKKKKDKG KDEVSKDKKK KKPTKDDKKG KSNGSGDSST DFSSDSTDSE DDLSNGPPKK 430 440 450 460 470 480 KVVVKDKDKE KEKEKESAAS SKVIASSSNA NKSRSATPTL STDASKRKMN SLPSDLTASD 490 500 510 520 530 540 TSNSPTSTPA KRPKNEISTS LPTSFSGGKV EDYGITEEAV RRYLKRKPLT ATELLTKFKN 550 560 570 KKTPVSSDRL VETMTKILKK INPVKHTIQG KMYLWIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of cDNA encoding Drosophila transcription initiation factor TFIIF alpha (RAP74)." Gong D.-W., Horikoshi M., Nakatani Y. Nucleic Acids Res. 21:1492-1492(1993) [PubMed: 8464745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of a Drosophila cDNA with sequence similarity to human transcription factor RAP74." Kephart D., Price M.P., Burton Z.F., Finkelstein A., Greenblalt J., Price D.H. Nucleic Acids Res. 21:1319-1319(1993) [PubMed: 8464716] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Functional analysis of Drosophila factor 5 (TFIIF), a general transcription factor." Kephart D., Wang B.Q., Burton Z.F., Price D.H. J. Biol. Chem. 269:13536-13543(1994) [PubMed: 8175788] [Abstract] Cited for: FUNCTION.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-246; SER-250; SER-252; THR-341; SER-342; SER-352; SER-355; SER-453; SER-455; THR-457; SER-482; SER-484 AND THR-488, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10331 mRNA. No translation available. X66982 mRNA. Translation: CAA47391.1. AE014297 Genomic DNA. Translation: AAF54125.1. AY051733 mRNA. Translation: AAK93157.1.
PIR	S30237.
RefSeq	NP_524246.1.
UniGene	Dm.6708
3D structure databases
HSSP	HSSP built from PDB template 1F3U based on UniProtKB P35269.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17887N.
IntAct	Q05913. 2 interactions.
Proteomic databases
PRIDE	Q05913.
Genome annotation databases
Ensembl	FBgn0010282. Drosophila melanogaster. [Contig view]
GeneID	40790.
KEGG	dme:Dmel_CG10281.
NMPDR	fig|7227.3.peg.11398.
Organism-specific databases
FlyBase	FBgn0010282. TfIIFalpha.
Phylogenomic databases
HOGENOM	Q05913.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-010266-MON.
Gene expression databases
ArrayExpress	Q05913.
GermOnline	CG10281. Drosophila melanogaster.
Family and domain databases
InterPro	IPR008851. TFIIF-alpha. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF05793. TFIIF_alpha. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	820599.

Entry information

Entry name

T2FA_DROME

Accession

Primary (citable) accession number: Q05913
Secondary accession number(s): Q9VI19

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project