Reviewed, UniProtKB/Swiss-Prot Q05911 (PUR8_YEAST)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate lyase
      Short name=ASL
    EC=4.3.2.2
Alternative name(s):
    Adenylosuccinase
      Short name=ASase

Gene names

Name:	ADE13
Ordered Locus Names:	YLR359W
ORF Names:	L8039.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.
Sequence similarities	Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Lyase
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from mutant phenotype. Source: SGD
Molecular function	(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPS1	P38148	1	EBI-14263,EBI-21161

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Adenylosuccinate lyase		PRO_0000137899
Sites
	Active site	83	1	Proton donor By similarity
	Active site	156	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	4	1	Phosphotyrosine Ref.3
	Modified residue	9	1	Phosphothreonine Ref.3
	Cross-link	196		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q05911-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AED9468B7828AB77	FASTA	482	54,510
	10 20 30 40 50 60 MPDYDNYTTP LSSRYASKEM SATFSLRNRF STWRKLWLNL AIAEKELGLT VVTDEAIEQM 70 80 90 100 110 120 RKHVEITDDE IAKASAQEAI VRHDVMAHVH TFGETCPAAA GIIHLGATSC FVTDNADLIF 130 140 150 160 170 180 IRDAYDIIIP KLVNVINRLA KFAMEYKDLP VLGWTHFQPA QLTTLGKRAT LWIQELLWDL 190 200 210 220 230 240 RNFERARNDI GLRGVKGTTG TQASFLALFH GNHDKVEALD ERVTELLGFD KVYPVTGQTY 250 260 270 280 290 300 SRKIDIDVLA PLSSFAATAH KMATDIRLLA NLKEVEEPFE KSQIGSSAMA YKRNPMRCER 310 320 330 340 350 360 VCSLARHLGS LFSDAVQTAS VQWFERTLDD SAIRRISLPS AFLTADILLS TLLNISSGLV 370 380 390 400 410 420 VYPKVIERRI KGELPFMATE NIIMAMVEKN ASRQEVHERI RVLSHQAAAV VKEEGGENDL 430 440 450 460 470 480 IERVKRDEFF KPIWEELDSL LEPSTFVGRA PQQVEKFVQK DVNNALQPFQ KYLNDEQVKL NV

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-196, MASS SPECTROMETRY.
[3]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-4 AND THR-9, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19103 Genomic DNA. Translation: AAB67573.1. U19102 Genomic DNA. Translation: AAB67755.2.
PIR	S51377.
RefSeq	NP_013463.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2787N.
IntAct	Q05911. 7 interactions.
STRING	Q05911.
Proteomic databases
PeptideAtlas	Q05911.
Genome annotation databases
Ensembl	YLR359W; YLR359W; YLR359W; Saccharomyces cerevisiae. [Genome view]
GeneID	851073.
KEGG	sce:YLR359W.
NMPDR	fig|4932.3.peg.4485.
Organism-specific databases
CYGD	YLR359w.
SGD	S000004351. ADE13.
Phylogenomic databases
HOGENOM	Q05911.
OMA	DDSAIRR
OrthoDB	EOG9DBVXF
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-489.
BRENDA	4.3.2.2. 250.
Gene expression databases
ArrayExpress	Q05911.
Genevestigator	Q05911.
GermOnline	YLR359W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019468. Adenylosuccinate_lyase_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR004769. Pur_lyase. [Graphical view]
Pfam	PF10397. ADSL_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00928. purB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967720.

Entry information

Entry name

PUR8_YEAST

Accession

Primary (citable) accession number: Q05911

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)