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Protein

Disintegrin and metalloproteinase domain-containing protein 8

Gene

Adam8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible involvement in extravasation of leukocytes.

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi329 – 3291Zinc; catalyticBy similarity
Active sitei330 – 3301PROSITE-ProRule annotation
Metal bindingi333 – 3331Zinc; catalyticBy similarity
Metal bindingi339 – 3391Zinc; catalyticBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: BHF-UCL
  2. cell adhesion molecule binding Source: BHF-UCL
  3. integrin binding Source: BHF-UCL
  4. metalloendopeptidase activity Source: InterPro
  5. metallopeptidase activity Source: BHF-UCL
  6. zinc ion binding Source: BHF-UCL

GO - Biological processi

  1. activation of MAPK activity involved in innate immune response Source: BHF-UCL
  2. angiogenesis Source: BHF-UCL
  3. blood vessel endothelial cell migration Source: BHF-UCL
  4. cell-matrix adhesion Source: BHF-UCL
  5. cell morphogenesis Source: BHF-UCL
  6. cellular response to hypoxia Source: BHF-UCL
  7. inflammatory response Source: MGI
  8. leukocyte migration involved in inflammatory response Source: BHF-UCL
  9. lymphocyte chemotaxis Source: BHF-UCL
  10. membrane fusion Source: BHF-UCL
  11. negative regulation of neuron apoptotic process Source: BHF-UCL
  12. negative regulation of thymocyte aggregation Source: BHF-UCL
  13. osteoclast fusion Source: BHF-UCL
  14. positive regulation of acute inflammatory response Source: BHF-UCL
  15. positive regulation of bone resorption Source: BHF-UCL
  16. positive regulation of cell adhesion Source: BHF-UCL
  17. positive regulation of cellular extravasation Source: BHF-UCL
  18. positive regulation of eosinophil migration Source: BHF-UCL
  19. positive regulation of fibronectin-dependent thymocyte migration Source: BHF-UCL
  20. positive regulation of inflammatory response Source: BHF-UCL
  21. positive regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
  22. positive regulation of neutrophil extravasation Source: MGI
  23. positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
  24. positive regulation of protein kinase B signaling Source: BHF-UCL
  25. positive regulation of T cell differentiation in thymus Source: BHF-UCL
  26. positive regulation of T cell migration Source: BHF-UCL
  27. positive regulation of thymocyte apoptotic process Source: BHF-UCL
  28. positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Source: BHF-UCL
  29. regulation of cell-cell adhesion Source: BHF-UCL
  30. regulation of osteoclast differentiation Source: BHF-UCL
  31. single organismal cell-cell adhesion Source: MGI
  32. tissue morphogenesis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_275864. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiM12.208.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 8 (EC:3.4.24.-)
Short name:
ADAM 8
Alternative name(s):
Cell surface antigen MS2
Macrophage cysteine-rich glycoprotein
CD_antigen: CD156a
Gene namesi
Name:Adam8
Synonyms:Ms2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:107825. Adam8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 658642ExtracellularSequence AnalysisAdd
BLAST
Transmembranei659 – 68325HelicalSequence AnalysisAdd
BLAST
Topological domaini684 – 826143CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. alpha9-beta1 integrin-ADAM8 complex Source: BHF-UCL
  2. alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex Source: Ensembl
  3. cell surface Source: MGI
  4. cytoplasm Source: BHF-UCL
  5. dense core granule membrane Source: BHF-UCL
  6. integral component of plasma membrane Source: BHF-UCL
  7. phagolysosome Source: BHF-UCL
  8. plasma membrane Source: BHF-UCL
  9. podosome Source: BHF-UCL
  10. specific granule Source: BHF-UCL
  11. tertiary granule Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 826810Disintegrin and metalloproteinase domain-containing protein 8PRO_0000029061Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi260 – 2601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi305 ↔ 390By similarity
Disulfide bondi346 ↔ 374By similarity
Disulfide bondi348 ↔ 357By similarity
Glycosylationi431 – 4311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi461 ↔ 481By similarity
Glycosylationi614 – 6141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi615 ↔ 625By similarity
Disulfide bondi619 ↔ 631By similarity
Disulfide bondi633 ↔ 642By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ05910.
PRIDEiQ05910.

PTM databases

PhosphoSiteiQ05910.

Miscellaneous databases

PMAP-CutDBQ05910.

Expressioni

Tissue specificityi

Macrophages.

Gene expression databases

BgeeiQ05910.
CleanExiMM_ADAM8.
ExpressionAtlasiQ05910. baseline and differential.
GenevestigatoriQ05910.

Interactioni

Subunit structurei

Interacts with FST3.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ05910.
SMRiQ05910. Positions 193-607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini196 – 395200Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini403 – 48987DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini611 – 64333EGF-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG310718.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ05910.
KOiK06540.
OMAiCYNGACP.
PhylomeDBiQ05910.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGLWLLSVL WTPAVAPGPP LPHVKQYEVV WPRRLAASRS RRALPSHWGQ
60 70 80 90 100
YPESLSYALG TSGHVFTLHL RKNRDLLGSS YTETYSAANG SEVTEQLQEQ
110 120 130 140 150
DHCLYQGHVE GYEGSAASIS TCAGLRGFFR VGSTVHLIEP LDADEEGQHA
160 170 180 190 200
MYQAKHLQQK AGTCGVKDTN LNDLGPRALE IYRAQPRNWL IPRETRYVEL
210 220 230 240 250
YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV VLVGLEIWNK
260 270 280 290 300
DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK
310 320 330 340 350
VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE
360 370 380 390 400
PREGGGCIMT ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF
410 420 430 440 450
VGGPVCGNLF VEHGEQCDCG TPQDCQNPCC NATTCQLVKG AECASGTCCH
460 470 480 490 500
ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT CPEDAFQQNG TPCPGGYCFD
510 520 530 540 550
GSCPTLAQQC RDLWGPGARV AADSCYTFSI PPGCNGRMYS GRINRCGALY
560 570 580 590 600
CEGGQKPLER SFCTFSSNHG VCHALGTGSN IDTFELVLQG TKCEEGKVCM
610 620 630 640 650
DGSCQDLRVY RSENCSAKCN NHGVCNHKRE CHCHKGWAPP NCVQRLADVS
660 670 680 690 700
DEQAASTSLP VSVVVVLVIL VAAMVIVAGI VIYRKAPRQI QRRSVAPKPI
710 720 730 740 750
SGLSNPLFYT RDSSLPAKNR PPDPSETVST NQPPRPIVKP KRPPPAPPGA
760 770 780 790 800
VSSSPLPVPV YAPKIPNQFR PDPPTKPLPE LKPKQVKPTF APPTPPVKPG
810 820
TGGTVPGATQ GAGEPKVALK VPIQKR
Length:826
Mass (Da):90,046
Last modified:October 1, 1996 - v3
Checksum:i3142CC81DBBADFB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10911 Genomic DNA. Translation: BAA21771.1.
X13335 mRNA. Translation: CAA31712.1.
CCDSiCCDS21959.1.
RefSeqiNP_031429.1. NM_007403.2.
UniGeneiMm.15969.

Genome annotation databases

EnsembliENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
GeneIDi11501.
KEGGimmu:11501.
UCSCiuc009kgj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10911 Genomic DNA. Translation: BAA21771.1.
X13335 mRNA. Translation: CAA31712.1.
CCDSiCCDS21959.1.
RefSeqiNP_031429.1. NM_007403.2.
UniGeneiMm.15969.

3D structure databases

ProteinModelPortaliQ05910.
SMRiQ05910. Positions 193-607.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM12.208.

PTM databases

PhosphoSiteiQ05910.

Proteomic databases

PaxDbiQ05910.
PRIDEiQ05910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
GeneIDi11501.
KEGGimmu:11501.
UCSCiuc009kgj.1. mouse.

Organism-specific databases

CTDi101.
MGIiMGI:107825. Adam8.

Phylogenomic databases

eggNOGiNOG310718.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ05910.
KOiK06540.
OMAiCYNGACP.
PhylomeDBiQ05910.
TreeFamiTF314733.

Enzyme and pathway databases

ReactomeiREACT_275864. Degradation of the extracellular matrix.

Miscellaneous databases

NextBioi278902.
PMAP-CutDBQ05910.
PROiQ05910.
SOURCEiSearch...

Gene expression databases

BgeeiQ05910.
CleanExiMM_ADAM8.
ExpressionAtlasiQ05910. baseline and differential.
GenevestigatoriQ05910.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Yamamoto S., Yoshiyama K., Setoguchi M., Matsuura K., Higuchi Y., Akizuki S.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ICR.
  2. "Structure of the murine CD156 gene, characterization of its promoter, and chromosomal location."
    Kataoka M., Yoshiyama K., Matsuura K., Hijiya N., Higuchi Y., Yamamoto S.
    J. Biol. Chem. 272:18209-18215(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Liver.
  3. "Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage."
    Yoshida S., Setoguchi M., Higuchi Y., Akizuki S., Yamamoto S.
    Int. Immunol. 2:585-591(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiADAM8_MOUSE
AccessioniPrimary (citable) accession number: Q05910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: April 1, 2015
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.