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Q05910

- ADAM8_MOUSE

UniProt

Q05910 - ADAM8_MOUSE

Protein

Disintegrin and metalloproteinase domain-containing protein 8

Gene

Adam8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Possible involvement in extravasation of leukocytes.

    Cofactori

    Binds 1 zinc ion per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi329 – 3291Zinc; catalyticBy similarity
    Active sitei330 – 3301PROSITE-ProRule annotation
    Metal bindingi333 – 3331Zinc; catalyticBy similarity
    Metal bindingi339 – 3391Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: BHF-UCL
    2. cell adhesion molecule binding Source: BHF-UCL
    3. integrin binding Source: BHF-UCL
    4. metalloendopeptidase activity Source: InterPro
    5. metallopeptidase activity Source: BHF-UCL
    6. protein binding Source: BHF-UCL
    7. zinc ion binding Source: BHF-UCL

    GO - Biological processi

    1. activation of MAPK activity involved in innate immune response Source: BHF-UCL
    2. angiogenesis Source: BHF-UCL
    3. blood vessel endothelial cell migration Source: BHF-UCL
    4. cell-matrix adhesion Source: BHF-UCL
    5. cell morphogenesis Source: BHF-UCL
    6. cellular response to hypoxia Source: BHF-UCL
    7. leukocyte migration involved in inflammatory response Source: BHF-UCL
    8. lymphocyte chemotaxis Source: BHF-UCL
    9. membrane fusion Source: BHF-UCL
    10. negative regulation of neuron apoptotic process Source: BHF-UCL
    11. negative regulation of thymocyte aggregation Source: BHF-UCL
    12. osteoclast fusion Source: BHF-UCL
    13. positive regulation of acute inflammatory response Source: BHF-UCL
    14. positive regulation of bone resorption Source: BHF-UCL
    15. positive regulation of cell adhesion Source: BHF-UCL
    16. positive regulation of cellular extravasation Source: BHF-UCL
    17. positive regulation of eosinophil migration Source: BHF-UCL
    18. positive regulation of fibronectin-dependent thymocyte migration Source: BHF-UCL
    19. positive regulation of inflammatory response Source: BHF-UCL
    20. positive regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
    21. positive regulation of neutrophil extravasation Source: Ensembl
    22. positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
    23. positive regulation of protein kinase B signaling Source: BHF-UCL
    24. positive regulation of T cell differentiation in thymus Source: BHF-UCL
    25. positive regulation of T cell migration Source: BHF-UCL
    26. positive regulation of thymocyte apoptotic process Source: BHF-UCL
    27. positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Source: BHF-UCL
    28. regulation of cell-cell adhesion Source: BHF-UCL
    29. regulation of osteoclast differentiation Source: BHF-UCL
    30. single organismal cell-cell adhesion Source: MGI
    31. tissue morphogenesis Source: BHF-UCL

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199052. Degradation of the extracellular matrix.

    Protein family/group databases

    MEROPSiM12.208.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 8 (EC:3.4.24.-)
    Short name:
    ADAM 8
    Alternative name(s):
    Cell surface antigen MS2
    Macrophage cysteine-rich glycoprotein
    CD_antigen: CD156a
    Gene namesi
    Name:Adam8
    Synonyms:Ms2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:107825. Adam8.

    Subcellular locationi

    GO - Cellular componenti

    1. alpha9-beta1 integrin-ADAM8 complex Source: BHF-UCL
    2. alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex Source: Ensembl
    3. cell surface Source: Ensembl
    4. cytoplasm Source: BHF-UCL
    5. dense core granule membrane Source: BHF-UCL
    6. integral component of plasma membrane Source: BHF-UCL
    7. phagolysosome Source: BHF-UCL
    8. plasma membrane Source: BHF-UCL
    9. podosome Source: BHF-UCL
    10. specific granule Source: BHF-UCL
    11. tertiary granule Source: BHF-UCL

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 826810Disintegrin and metalloproteinase domain-containing protein 8PRO_0000029061Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi260 – 2601N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi305 ↔ 390By similarity
    Disulfide bondi346 ↔ 374By similarity
    Disulfide bondi348 ↔ 357By similarity
    Glycosylationi431 – 4311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi461 ↔ 481By similarity
    Glycosylationi614 – 6141N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi615 ↔ 625By similarity
    Disulfide bondi619 ↔ 631By similarity
    Disulfide bondi633 ↔ 642By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ05910.
    PRIDEiQ05910.

    PTM databases

    PhosphoSiteiQ05910.

    Miscellaneous databases

    PMAP-CutDBQ05910.

    Expressioni

    Tissue specificityi

    Macrophages.

    Gene expression databases

    BgeeiQ05910.
    CleanExiMM_ADAM8.
    GenevestigatoriQ05910.

    Interactioni

    Subunit structurei

    Interacts with FST3.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ05910.
    SMRiQ05910. Positions 193-646.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 658642ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini684 – 826143CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei659 – 68325HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini196 – 395200Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini403 – 48987DisintegrinPROSITE-ProRule annotationAdd
    BLAST
    Domaini611 – 64333EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 disintegrin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG310718.
    HOGENOMiHOG000230883.
    HOVERGENiHBG006978.
    KOiK06540.
    OMAiCYNGACP.
    PhylomeDBiQ05910.
    TreeFamiTF314733.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiIPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view]
    PfamiPF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view]
    PRINTSiPR00289. DISINTEGRIN.
    SMARTiSM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05910-1 [UniParc]FASTAAdd to Basket

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    MLGLWLLSVL WTPAVAPGPP LPHVKQYEVV WPRRLAASRS RRALPSHWGQ    50
    YPESLSYALG TSGHVFTLHL RKNRDLLGSS YTETYSAANG SEVTEQLQEQ 100
    DHCLYQGHVE GYEGSAASIS TCAGLRGFFR VGSTVHLIEP LDADEEGQHA 150
    MYQAKHLQQK AGTCGVKDTN LNDLGPRALE IYRAQPRNWL IPRETRYVEL 200
    YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV VLVGLEIWNK 250
    DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK 300
    VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE 350
    PREGGGCIMT ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF 400
    VGGPVCGNLF VEHGEQCDCG TPQDCQNPCC NATTCQLVKG AECASGTCCH 450
    ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT CPEDAFQQNG TPCPGGYCFD 500
    GSCPTLAQQC RDLWGPGARV AADSCYTFSI PPGCNGRMYS GRINRCGALY 550
    CEGGQKPLER SFCTFSSNHG VCHALGTGSN IDTFELVLQG TKCEEGKVCM 600
    DGSCQDLRVY RSENCSAKCN NHGVCNHKRE CHCHKGWAPP NCVQRLADVS 650
    DEQAASTSLP VSVVVVLVIL VAAMVIVAGI VIYRKAPRQI QRRSVAPKPI 700
    SGLSNPLFYT RDSSLPAKNR PPDPSETVST NQPPRPIVKP KRPPPAPPGA 750
    VSSSPLPVPV YAPKIPNQFR PDPPTKPLPE LKPKQVKPTF APPTPPVKPG 800
    TGGTVPGATQ GAGEPKVALK VPIQKR 826
    Length:826
    Mass (Da):90,046
    Last modified:October 1, 1996 - v3
    Checksum:i3142CC81DBBADFB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10911 Genomic DNA. Translation: BAA21771.1.
    X13335 mRNA. Translation: CAA31712.1.
    CCDSiCCDS21959.1.
    RefSeqiNP_031429.1. NM_007403.2.
    UniGeneiMm.15969.

    Genome annotation databases

    EnsembliENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
    GeneIDi11501.
    KEGGimmu:11501.
    UCSCiuc009kgj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10911 Genomic DNA. Translation: BAA21771.1 .
    X13335 mRNA. Translation: CAA31712.1 .
    CCDSi CCDS21959.1.
    RefSeqi NP_031429.1. NM_007403.2.
    UniGenei Mm.15969.

    3D structure databases

    ProteinModelPortali Q05910.
    SMRi Q05910. Positions 193-646.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.208.

    PTM databases

    PhosphoSitei Q05910.

    Proteomic databases

    PaxDbi Q05910.
    PRIDEi Q05910.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000106069 ; ENSMUSP00000101684 ; ENSMUSG00000025473 .
    GeneIDi 11501.
    KEGGi mmu:11501.
    UCSCi uc009kgj.1. mouse.

    Organism-specific databases

    CTDi 101.
    MGIi MGI:107825. Adam8.

    Phylogenomic databases

    eggNOGi NOG310718.
    HOGENOMi HOG000230883.
    HOVERGENi HBG006978.
    KOi K06540.
    OMAi CYNGACP.
    PhylomeDBi Q05910.
    TreeFami TF314733.

    Enzyme and pathway databases

    Reactomei REACT_199052. Degradation of the extracellular matrix.

    Miscellaneous databases

    NextBioi 278902.
    PMAP-CutDB Q05910.
    PROi Q05910.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q05910.
    CleanExi MM_ADAM8.
    Genevestigatori Q05910.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProi IPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view ]
    Pfami PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view ]
    PRINTSi PR00289. DISINTEGRIN.
    SMARTi SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57552. SSF57552. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Yamamoto S., Yoshiyama K., Setoguchi M., Matsuura K., Higuchi Y., Akizuki S.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ICR.
    2. "Structure of the murine CD156 gene, characterization of its promoter, and chromosomal location."
      Kataoka M., Yoshiyama K., Matsuura K., Hijiya N., Higuchi Y., Yamamoto S.
      J. Biol. Chem. 272:18209-18215(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Liver.
    3. "Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage."
      Yoshida S., Setoguchi M., Higuchi Y., Akizuki S., Yamamoto S.
      Int. Immunol. 2:585-591(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiADAM8_MOUSE
    AccessioniPrimary (citable) accession number: Q05910
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3