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Protein

Disintegrin and metalloproteinase domain-containing protein 8

Gene

Adam8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible involvement in extravasation of leukocytes.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi329Zinc; catalyticBy similarity1
Active sitei330PROSITE-ProRule annotation1
Metal bindingi333Zinc; catalyticBy similarity1
Metal bindingi339Zinc; catalyticBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: BHF-UCL
  • cell adhesion molecule binding Source: BHF-UCL
  • integrin binding Source: BHF-UCL
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: BHF-UCL
  • zinc ion binding Source: BHF-UCL

GO - Biological processi

  • activation of MAPK activity involved in innate immune response Source: BHF-UCL
  • angiogenesis Source: BHF-UCL
  • blood vessel endothelial cell migration Source: BHF-UCL
  • cell-matrix adhesion Source: BHF-UCL
  • cell morphogenesis Source: BHF-UCL
  • cellular response to hypoxia Source: BHF-UCL
  • inflammatory response Source: MGI
  • leukocyte migration involved in inflammatory response Source: BHF-UCL
  • lymphocyte chemotaxis Source: BHF-UCL
  • membrane fusion Source: BHF-UCL
  • negative regulation of neuron apoptotic process Source: BHF-UCL
  • negative regulation of thymocyte aggregation Source: BHF-UCL
  • osteoclast fusion Source: BHF-UCL
  • positive regulation of acute inflammatory response Source: BHF-UCL
  • positive regulation of bone resorption Source: BHF-UCL
  • positive regulation of cell adhesion Source: BHF-UCL
  • positive regulation of cellular extravasation Source: BHF-UCL
  • positive regulation of eosinophil migration Source: BHF-UCL
  • positive regulation of fibronectin-dependent thymocyte migration Source: BHF-UCL
  • positive regulation of inflammatory response Source: BHF-UCL
  • positive regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
  • positive regulation of neutrophil extravasation Source: MGI
  • positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
  • positive regulation of protein kinase B signaling Source: BHF-UCL
  • positive regulation of T cell differentiation in thymus Source: BHF-UCL
  • positive regulation of T cell migration Source: BHF-UCL
  • positive regulation of thymocyte apoptotic process Source: BHF-UCL
  • positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Source: BHF-UCL
  • regulation of cell-cell adhesion Source: BHF-UCL
  • regulation of osteoclast differentiation Source: BHF-UCL
  • single organismal cell-cell adhesion Source: MGI
  • tissue morphogenesis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiM12.208.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 8 (EC:3.4.24.-)
Short name:
ADAM 8
Alternative name(s):
Cell surface antigen MS2
Macrophage cysteine-rich glycoprotein
CD_antigen: CD156a
Gene namesi
Name:Adam8
Synonyms:Ms2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:107825. Adam8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 658ExtracellularSequence analysisAdd BLAST642
Transmembranei659 – 683HelicalSequence analysisAdd BLAST25
Topological domaini684 – 826CytoplasmicSequence analysisAdd BLAST143

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000002906117 – 826Disintegrin and metalloproteinase domain-containing protein 8Add BLAST810

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi260N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi305 ↔ 390By similarity
Disulfide bondi346 ↔ 374By similarity
Disulfide bondi348 ↔ 357By similarity
Disulfide bondi430 ↔ 452By similarity
Glycosylationi431N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi443 ↔ 449By similarity
Disulfide bondi461 ↔ 481By similarity
Disulfide bondi468 ↔ 498By similarity
Disulfide bondi493 ↔ 503By similarity
Disulfide bondi563 ↔ 615By similarity
Glycosylationi614N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi615 ↔ 625By similarity
Disulfide bondi619 ↔ 631By similarity
Disulfide bondi633 ↔ 642By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ05910.
PaxDbiQ05910.
PeptideAtlasiQ05910.
PRIDEiQ05910.

PTM databases

iPTMnetiQ05910.
PhosphoSitePlusiQ05910.

Miscellaneous databases

PMAP-CutDBQ05910.

Expressioni

Tissue specificityi

Macrophages.

Gene expression databases

BgeeiENSMUSG00000025473.
CleanExiMM_ADAM8.
ExpressionAtlasiQ05910. baseline and differential.
GenevisibleiQ05910. MM.

Interactioni

Subunit structurei

Interacts with FST3.By similarity

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • integrin binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101684.

Structurei

3D structure databases

ProteinModelPortaliQ05910.
SMRiQ05910.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini196 – 395Peptidase M12BPROSITE-ProRule annotationAdd BLAST200
Domaini403 – 489DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini611 – 643EGF-likePROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ05910.
KOiK06540.
OMAiCYNGACP.
OrthoDBiEOG091G01NX.
PhylomeDBiQ05910.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGLWLLSVL WTPAVAPGPP LPHVKQYEVV WPRRLAASRS RRALPSHWGQ
60 70 80 90 100
YPESLSYALG TSGHVFTLHL RKNRDLLGSS YTETYSAANG SEVTEQLQEQ
110 120 130 140 150
DHCLYQGHVE GYEGSAASIS TCAGLRGFFR VGSTVHLIEP LDADEEGQHA
160 170 180 190 200
MYQAKHLQQK AGTCGVKDTN LNDLGPRALE IYRAQPRNWL IPRETRYVEL
210 220 230 240 250
YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV VLVGLEIWNK
260 270 280 290 300
DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK
310 320 330 340 350
VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE
360 370 380 390 400
PREGGGCIMT ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF
410 420 430 440 450
VGGPVCGNLF VEHGEQCDCG TPQDCQNPCC NATTCQLVKG AECASGTCCH
460 470 480 490 500
ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT CPEDAFQQNG TPCPGGYCFD
510 520 530 540 550
GSCPTLAQQC RDLWGPGARV AADSCYTFSI PPGCNGRMYS GRINRCGALY
560 570 580 590 600
CEGGQKPLER SFCTFSSNHG VCHALGTGSN IDTFELVLQG TKCEEGKVCM
610 620 630 640 650
DGSCQDLRVY RSENCSAKCN NHGVCNHKRE CHCHKGWAPP NCVQRLADVS
660 670 680 690 700
DEQAASTSLP VSVVVVLVIL VAAMVIVAGI VIYRKAPRQI QRRSVAPKPI
710 720 730 740 750
SGLSNPLFYT RDSSLPAKNR PPDPSETVST NQPPRPIVKP KRPPPAPPGA
760 770 780 790 800
VSSSPLPVPV YAPKIPNQFR PDPPTKPLPE LKPKQVKPTF APPTPPVKPG
810 820
TGGTVPGATQ GAGEPKVALK VPIQKR
Length:826
Mass (Da):90,046
Last modified:October 1, 1996 - v3
Checksum:i3142CC81DBBADFB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10911 Genomic DNA. Translation: BAA21771.1.
X13335 mRNA. Translation: CAA31712.1.
CCDSiCCDS21959.1.
RefSeqiNP_031429.1. NM_007403.3.
UniGeneiMm.15969.

Genome annotation databases

EnsembliENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
GeneIDi11501.
KEGGimmu:11501.
UCSCiuc009kgj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10911 Genomic DNA. Translation: BAA21771.1.
X13335 mRNA. Translation: CAA31712.1.
CCDSiCCDS21959.1.
RefSeqiNP_031429.1. NM_007403.3.
UniGeneiMm.15969.

3D structure databases

ProteinModelPortaliQ05910.
SMRiQ05910.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101684.

Protein family/group databases

MEROPSiM12.208.

PTM databases

iPTMnetiQ05910.
PhosphoSitePlusiQ05910.

Proteomic databases

EPDiQ05910.
PaxDbiQ05910.
PeptideAtlasiQ05910.
PRIDEiQ05910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
GeneIDi11501.
KEGGimmu:11501.
UCSCiuc009kgj.2. mouse.

Organism-specific databases

CTDi101.
MGIiMGI:107825. Adam8.

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ05910.
KOiK06540.
OMAiCYNGACP.
OrthoDBiEOG091G01NX.
PhylomeDBiQ05910.
TreeFamiTF314733.

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PMAP-CutDBQ05910.
PROiQ05910.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025473.
CleanExiMM_ADAM8.
ExpressionAtlasiQ05910. baseline and differential.
GenevisibleiQ05910. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADAM8_MOUSE
AccessioniPrimary (citable) accession number: Q05910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.