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Q05910 (ADAM8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 8
Short name=ADAM 8
EC=3.4.24.-
Alternative name(s):
Cell surface antigen MS2
Macrophage cysteine-rich glycoprotein
CD_antigen=CD156a
Gene names
Name:Adam8
Synonyms:Ms2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Possible involvement in extravasation of leukocytes.

Cofactor

Binds 1 zinc ion per subunit Probable.

Subunit structure

Interacts with FST3 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Macrophages.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processactivation of MAPK activity involved in innate immune response

Inferred from direct assay. Source: BHF-UCL

angiogenesis

Inferred from direct assay. Source: BHF-UCL

blood vessel endothelial cell migration

Non-traceable author statement. Source: BHF-UCL

cell morphogenesis

Inferred from mutant phenotype. Source: BHF-UCL

cell-matrix adhesion

Traceable author statement. Source: BHF-UCL

cellular membrane fusion

Traceable author statement. Source: BHF-UCL

cellular response to hypoxia

Inferred from sequence or structural similarity. Source: BHF-UCL

leukocyte migration involved in inflammatory response

Inferred from mutant phenotype. Source: BHF-UCL

lymphocyte chemotaxis

Inferred from direct assay. Source: BHF-UCL

negative regulation of neuron apoptosis

Inferred from direct assay. Source: BHF-UCL

positive regulation of NF-kappaB transcription factor activity

Inferred from direct assay. Source: BHF-UCL

positive regulation of T cell differentiation in thymus

Inferred from direct assay. Source: BHF-UCL

positive regulation of acute inflammatory response

Inferred from mutant phenotype. Source: BHF-UCL

positive regulation of bone resorption

Inferred from direct assay. Source: BHF-UCL

positive regulation of cell adhesion

Inferred from direct assay. Source: BHF-UCL

positive regulation of cellular extravasation

Inferred from mutant phenotype. Source: BHF-UCL

positive regulation of membrane protein ectodomain proteolysis

Inferred from direct assay. Source: BHF-UCL

positive regulation of protein kinase B signaling cascade

Inferred from direct assay. Source: BHF-UCL

positive regulation of thymocyte apoptosis

Inferred from mutant phenotype. Source: BHF-UCL

positive regulation of tumor necrosis factor (ligand) superfamily member 11 production

Inferred from mutant phenotype. Source: BHF-UCL

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cell-cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of osteoclast differentiation

Non-traceable author statement. Source: BHF-UCL

thymocyte aggregation

Non-traceable author statement. Source: BHF-UCL

tissue morphogenesis

Traceable author statement. Source: BHF-UCL

   Cellular componentalpha9-beta1 integrin-ADAM8 complex

Inferred from direct assay. Source: BHF-UCL

dense core granule membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

integral to plasma membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

phagolysosome

Inferred from sequence or structural similarity. Source: BHF-UCL

podosome

Inferred from sequence or structural similarity. Source: BHF-UCL

specific granule

Inferred from sequence or structural similarity. Source: BHF-UCL

tertiary granule

Inferred from sequence or structural similarity. Source: BHF-UCL

   Molecular functioncalcium ion binding

Inferred from direct assay. Source: BHF-UCL

cell adhesion molecule binding

Inferred from physical interaction. Source: BHF-UCL

integrin binding

Traceable author statement. Source: BHF-UCL

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from direct assay. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 826810Disintegrin and metalloproteinase domain-containing protein 8
PRO_0000029061

Regions

Topological domain17 – 658642Extracellular Potential
Transmembrane659 – 68325Helical; Potential
Topological domain684 – 826143Cytoplasmic Potential
Domain196 – 395200Peptidase M12B
Domain403 – 48987Disintegrin

Sites

Active site3301 By similarity
Metal binding3291Zinc; catalytic Probable
Metal binding3331Zinc; catalytic Probable
Metal binding3391Zinc; catalytic Probable

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation2601N-linked (GlcNAc...) Potential
Glycosylation4311N-linked (GlcNAc...) Potential
Glycosylation6141N-linked (GlcNAc...) Potential
Disulfide bond305 ↔ 390 By similarity
Disulfide bond346 ↔ 374 By similarity
Disulfide bond348 ↔ 357 By similarity
Disulfide bond461 ↔ 481 By similarity
Disulfide bond615 ↔ 625 By similarity
Disulfide bond619 ↔ 631 By similarity
Disulfide bond633 ↔ 642 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05910 [UniParc].

Last modified October 1, 1996. Version 3.
Checksum: 3142CC81DBBADFB9

FASTA82690,046
        10         20         30         40         50         60 
MLGLWLLSVL WTPAVAPGPP LPHVKQYEVV WPRRLAASRS RRALPSHWGQ YPESLSYALG 

        70         80         90        100        110        120 
TSGHVFTLHL RKNRDLLGSS YTETYSAANG SEVTEQLQEQ DHCLYQGHVE GYEGSAASIS 

       130        140        150        160        170        180 
TCAGLRGFFR VGSTVHLIEP LDADEEGQHA MYQAKHLQQK AGTCGVKDTN LNDLGPRALE 

       190        200        210        220        230        240 
IYRAQPRNWL IPRETRYVEL YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV 

       250        260        270        280        290        300 
VLVGLEIWNK DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK 

       310        320        330        340        350        360 
VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE PREGGGCIMT 

       370        380        390        400        410        420 
ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF VGGPVCGNLF VEHGEQCDCG 

       430        440        450        460        470        480 
TPQDCQNPCC NATTCQLVKG AECASGTCCH ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT 

       490        500        510        520        530        540 
CPEDAFQQNG TPCPGGYCFD GSCPTLAQQC RDLWGPGARV AADSCYTFSI PPGCNGRMYS 

       550        560        570        580        590        600 
GRINRCGALY CEGGQKPLER SFCTFSSNHG VCHALGTGSN IDTFELVLQG TKCEEGKVCM 

       610        620        630        640        650        660 
DGSCQDLRVY RSENCSAKCN NHGVCNHKRE CHCHKGWAPP NCVQRLADVS DEQAASTSLP 

       670        680        690        700        710        720 
VSVVVVLVIL VAAMVIVAGI VIYRKAPRQI QRRSVAPKPI SGLSNPLFYT RDSSLPAKNR 

       730        740        750        760        770        780 
PPDPSETVST NQPPRPIVKP KRPPPAPPGA VSSSPLPVPV YAPKIPNQFR PDPPTKPLPE 

       790        800        810        820 
LKPKQVKPTF APPTPPVKPG TGGTVPGATQ GAGEPKVALK VPIQKR

« Hide

References

[1]Yamamoto S., Yoshiyama K., Setoguchi M., Matsuura K., Higuchi Y., Akizuki S.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ICR.
[2]"Structure of the murine CD156 gene, characterization of its promoter, and chromosomal location."
Kataoka M., Yoshiyama K., Matsuura K., Hijiya N., Higuchi Y., Yamamoto S.
J. Biol. Chem. 272:18209-18215(1997) [PubMed: 9218457] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Liver.
[3]"Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage."
Yoshida S., Setoguchi M., Higuchi Y., Akizuki S., Yamamoto S.
Int. Immunol. 2:585-591(1990) [PubMed: 1982220] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D10911 Genomic DNA. Translation: BAA21771.1.
X13335 mRNA. Translation: CAA31712.1.
IPIIPI00114687.
RefSeqNP_031429.1. NM_007403.2.
UniGeneMm.15969.

3D structure databases

ProteinModelPortalQ05910.
SMRQ05910. Positions 185-648.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ05910.

Protein family/group databases

MEROPSM12.208.

PTM databases

PhosphoSiteQ05910.

Proteomic databases

PRIDEQ05910.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
GeneID11501.
KEGGmmu:11501.

Organism-specific databases

CTD101.
MGIMGI:107825. Adam8.

Phylogenomic databases

eggNOGmaNOG17283.
HOVERGENHBG006978.

Gene expression databases

ArrayExpressQ05910.
BgeeQ05910.
CleanExMM_ADAM8.
GenevestigatorQ05910.
GermOnlineENSMUSG00000025473. Mus musculus.

Family and domain databases

InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR006210. EGF-like.
IPR013032. EGF-like_reg_CS.
IPR000742. EGF_3.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
Gene3DG3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
KOK06540.
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF57552. Disintegrin. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278902.
PMAP-CutDBQ05910.
SOURCESearch...

Entry information

Entry nameADAM8_MOUSE
AccessionPrimary (citable) accession number: Q05910
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 16, 2011
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents