Reviewed, UniProtKB/Swiss-Prot Q05910 (ADAM8_MOUSE)

Names and origin

Protein names

Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 8
      Short name=ADAM 8
    EC=3.4.24.-
Alternative name(s):
    Cell surface antigen MS2
    Macrophage cysteine-rich glycoprotein
    CD_antigen=CD156a

Gene names

Name:	Adam8
Synonyms:	Ms2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Possible involvement in extravasation of leukocytes.
Cofactor	Binds 1 zinc ion per subunit Probable.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Macrophages.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	EGF-like domain Signal Transmembrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cell-cell adhesion Inferred from direct assay. Source: MGI proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: MGI
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 826	810	Disintegrin and metalloproteinase domain-containing protein 8		PRO_0000029061
Regions
	Topological domain	17 – 658	642	Extracellular Potential
	Transmembrane	659 – 683	25	Potential
	Topological domain	684 – 826	143	Cytoplasmic Potential
	Domain	196 – 395	200	Peptidase M12B
	Domain	403 – 489	87	Disintegrin
Sites
	Active site	330	1	By similarity
	Metal binding	329	1	Zinc; catalytic Probable
	Metal binding	333	1	Zinc; catalytic Probable
	Metal binding	339	1	Zinc; catalytic Probable
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Glycosylation	431	1	N-linked (GlcNAc...) Potential
	Glycosylation	614	1	N-linked (GlcNAc...) Potential
	Disulfide bond	305 ↔ 390		By similarity
	Disulfide bond	346 ↔ 374		By similarity
	Disulfide bond	348 ↔ 357		By similarity
	Disulfide bond	461 ↔ 481		By similarity
	Disulfide bond	615 ↔ 625		By similarity
	Disulfide bond	619 ↔ 631		By similarity
	Disulfide bond	633 ↔ 642		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05910-1 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 3142CC81DBBADFB9	FASTA	826	90,046
	10 20 30 40 50 60 MLGLWLLSVL WTPAVAPGPP LPHVKQYEVV WPRRLAASRS RRALPSHWGQ YPESLSYALG 70 80 90 100 110 120 TSGHVFTLHL RKNRDLLGSS YTETYSAANG SEVTEQLQEQ DHCLYQGHVE GYEGSAASIS 130 140 150 160 170 180 TCAGLRGFFR VGSTVHLIEP LDADEEGQHA MYQAKHLQQK AGTCGVKDTN LNDLGPRALE 190 200 210 220 230 240 IYRAQPRNWL IPRETRYVEL YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV 250 260 270 280 290 300 VLVGLEIWNK DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK 310 320 330 340 350 360 VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE PREGGGCIMT 370 380 390 400 410 420 ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF VGGPVCGNLF VEHGEQCDCG 430 440 450 460 470 480 TPQDCQNPCC NATTCQLVKG AECASGTCCH ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT 490 500 510 520 530 540 CPEDAFQQNG TPCPGGYCFD GSCPTLAQQC RDLWGPGARV AADSCYTFSI PPGCNGRMYS 550 560 570 580 590 600 GRINRCGALY CEGGQKPLER SFCTFSSNHG VCHALGTGSN IDTFELVLQG TKCEEGKVCM 610 620 630 640 650 660 DGSCQDLRVY RSENCSAKCN NHGVCNHKRE CHCHKGWAPP NCVQRLADVS DEQAASTSLP 670 680 690 700 710 720 VSVVVVLVIL VAAMVIVAGI VIYRKAPRQI QRRSVAPKPI SGLSNPLFYT RDSSLPAKNR 730 740 750 760 770 780 PPDPSETVST NQPPRPIVKP KRPPPAPPGA VSSSPLPVPV YAPKIPNQFR PDPPTKPLPE 790 800 810 820 LKPKQVKPTF APPTPPVKPG TGGTVPGATQ GAGEPKVALK VPIQKR

References

[1]	Yamamoto S., Yoshiyama K., Setoguchi M., Matsuura K., Higuchi Y., Akizuki S. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ICR.
[2]	"Structure of the murine CD156 gene, characterization of its promoter, and chromosomal location." Kataoka M., Yoshiyama K., Matsuura K., Hijiya N., Higuchi Y., Yamamoto S. J. Biol. Chem. 272:18209-18215(1997) [PubMed: 9218457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Liver.
[3]	"Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage." Yoshida S., Setoguchi M., Higuchi Y., Akizuki S., Yamamoto S. Int. Immunol. 2:585-591(1990) [PubMed: 1982220] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10911 Genomic DNA. Translation: BAA21771.1. X13335 mRNA. Translation: CAA31712.1.
IPI	IPI00114687.
RefSeq	NP_031429.1.
UniGene	Mm.15969
3D structure databases
HSSP	HSSP built from PDB template 1FVL based on UniProtKB P18619.
ModBase	Search...
Protein family/group databases
MEROPS	M12.208.
Proteomic databases
PRIDE	Q05910.
Genome annotation databases
Ensembl	ENSMUSG00000025473. Mus musculus. [Contig view]
GeneID	11501.
KEGG	mmu:11501.
Organism-specific databases
MGI	MGI:107825. Adam8.
Phylogenomic databases
HOGENOM	Q05910.
HOVERGEN	Q05910.
Gene expression databases
ArrayExpress	Q05910.
Bgee	Q05910.
CleanEx	MM_ADAM8.
GermOnline	ENSMUSG00000025473. Mus musculus.
Family and domain databases
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR013111. EGF_extracell. IPR006025. Pept_M_Zn_BS. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Gene3D	G3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF07974. EGF_2. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
PRINTS	PR00289. DISINTEGRIN.
ProDom	PD000664. Disintegrin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	278902.
PMAP-CutDB	Q05910.
SOURCE	Search...

Entry information

Entry name

ADAM8_MOUSE

Accession

Primary (citable) accession number: Q05910

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)