Q05910 (ADAM8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Disintegrin and metalloproteinase domain-containing protein 8
Short name=ADAM 8
EC=3.4.24.-

Alternative name(s):
Cell surface antigen MS2
Macrophage cysteine-rich glycoprotein
CD_antigen=CD156a

Gene names

Name:	Adam8
Synonyms:	Ms2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possible involvement in extravasation of leukocytes.
Cofactor	Binds 1 zinc ion per subunit Probable.
Subunit structure	Interacts with FST3 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Macrophages.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity involved in innate immune response Inferred from direct assay PubMed 20683884. Source: BHF-UCL angiogenesis Inferred from direct assay PubMed 19003792. Source: BHF-UCL blood vessel endothelial cell migration Non-traceable author statement PubMed 19003792. Source: BHF-UCL cell morphogenesis Inferred from mutant phenotype PubMed 11341326PubMed 20683884. Source: BHF-UCL cell-cell adhesion Inferred from direct assay PubMed 12372841. Source: MGI cell-matrix adhesion Traceable author statement PubMed 11341326. Source: BHF-UCL cellular membrane fusion Traceable author statement PubMed 11341326. Source: BHF-UCL cellular response to hypoxia Inferred from sequence or structural similarity. Source: BHF-UCL leukocyte migration involved in inflammatory response Inferred from mutant phenotype PubMed 20194813. Source: BHF-UCL lymphocyte chemotaxis Inferred from direct assay PubMed 20856819. Source: BHF-UCL negative regulation of neuron apoptotic process Inferred from direct assay PubMed 14761956. Source: BHF-UCL negative regulation of thymocyte aggregation Non-traceable author statement PubMed 20856819. Source: BHF-UCL osteoclast fusion Traceable author statement PubMed 12372841. Source: BHF-UCL positive regulation of NF-kappaB transcription factor activity Inferred from direct assay PubMed 20683884. Source: BHF-UCL positive regulation of T cell differentiation in thymus Inferred from direct assay PubMed 20856819. Source: BHF-UCL positive regulation of acute inflammatory response Inferred from mutant phenotype PubMed 21268008. Source: BHF-UCL positive regulation of bone resorption Inferred from direct assay PubMed 11341326. Source: BHF-UCL positive regulation of cell adhesion Inferred from direct assay PubMed 12372841. Source: BHF-UCL positive regulation of cellular extravasation Inferred from mutant phenotype PubMed 20194813. Source: BHF-UCL positive regulation of eosinophil migration Inferred from mutant phenotype PubMed 20194813. Source: BHF-UCL positive regulation of fibronectin-dependent thymocyte migration Inferred from mutant phenotype PubMed 20856819. Source: BHF-UCL positive regulation of membrane protein ectodomain proteolysis Inferred from direct assay PubMed 14761956. Source: BHF-UCL positive regulation of neutrophil extravasation Inferred from electronic annotation. Source: Compara positive regulation of protein kinase B signaling cascade Inferred from direct assay PubMed 20683884. Source: BHF-UCL positive regulation of thymocyte apoptotic process Inferred from mutant phenotype PubMed 20856819. Source: BHF-UCL positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Inferred from mutant phenotype PubMed 20683884. Source: BHF-UCL proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of osteoclast differentiation Non-traceable author statement PubMed 18397961. Source: BHF-UCL tissue morphogenesis Traceable author statement PubMed 11341326. Source: BHF-UCL
Cellular_component	alpha9-beta1 integrin-ADAM8 complex Inferred from direct assay PubMed 16995821. Source: BHF-UCL alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex Inferred from electronic annotation. Source: Compara cell surface Inferred from electronic annotation. Source: Compara dense core granule membrane Inferred from sequence or structural similarity. Source: BHF-UCL integral to plasma membrane Inferred from sequence or structural similarity. Source: BHF-UCL phagolysosome Inferred from sequence or structural similarity. Source: BHF-UCL podosome Inferred from sequence or structural similarity. Source: BHF-UCL specific granule Inferred from sequence or structural similarity. Source: BHF-UCL tertiary granule Inferred from sequence or structural similarity. Source: BHF-UCL
Molecular_function	calcium ion binding Inferred from direct assay PubMed 12372841. Source: BHF-UCL integrin binding Traceable author statement PubMed 16831910. Source: BHF-UCL metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from direct assay PubMed 12135759PubMed 14761956. Source: BHF-UCL zinc ion binding Inferred from direct assay PubMed 12135759PubMed 12372841. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 826	810	Disintegrin and metalloproteinase domain-containing protein 8		PRO_0000029061
Regions
	Topological domain	17 – 658	642	Extracellular Potential
	Transmembrane	659 – 683	25	Helical; Potential
	Topological domain	684 – 826	143	Cytoplasmic Potential
	Domain	196 – 395	200	Peptidase M12B
	Domain	403 – 489	87	Disintegrin
	Domain	611 – 643	33	EGF-like
Sites
	Active site	330	1	By similarity
	Metal binding	329	1	Zinc; catalytic By similarity
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	339	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Glycosylation	431	1	N-linked (GlcNAc...) Potential
	Glycosylation	614	1	N-linked (GlcNAc...) Potential
	Disulfide bond	305 ↔ 390		By similarity
	Disulfide bond	346 ↔ 374		By similarity
	Disulfide bond	348 ↔ 357		By similarity
	Disulfide bond	461 ↔ 481		By similarity
	Disulfide bond	615 ↔ 625		By similarity
	Disulfide bond	619 ↔ 631		By similarity
	Disulfide bond	633 ↔ 642		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05910 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 3142CC81DBBADFB9	FASTA	826	90,046
	10 20 30 40 50 60 MLGLWLLSVL WTPAVAPGPP LPHVKQYEVV WPRRLAASRS RRALPSHWGQ YPESLSYALG 70 80 90 100 110 120 TSGHVFTLHL RKNRDLLGSS YTETYSAANG SEVTEQLQEQ DHCLYQGHVE GYEGSAASIS 130 140 150 160 170 180 TCAGLRGFFR VGSTVHLIEP LDADEEGQHA MYQAKHLQQK AGTCGVKDTN LNDLGPRALE 190 200 210 220 230 240 IYRAQPRNWL IPRETRYVEL YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV 250 260 270 280 290 300 VLVGLEIWNK DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK 310 320 330 340 350 360 VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE PREGGGCIMT 370 380 390 400 410 420 ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF VGGPVCGNLF VEHGEQCDCG 430 440 450 460 470 480 TPQDCQNPCC NATTCQLVKG AECASGTCCH ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT 490 500 510 520 530 540 CPEDAFQQNG TPCPGGYCFD GSCPTLAQQC RDLWGPGARV AADSCYTFSI PPGCNGRMYS 550 560 570 580 590 600 GRINRCGALY CEGGQKPLER SFCTFSSNHG VCHALGTGSN IDTFELVLQG TKCEEGKVCM 610 620 630 640 650 660 DGSCQDLRVY RSENCSAKCN NHGVCNHKRE CHCHKGWAPP NCVQRLADVS DEQAASTSLP 670 680 690 700 710 720 VSVVVVLVIL VAAMVIVAGI VIYRKAPRQI QRRSVAPKPI SGLSNPLFYT RDSSLPAKNR 730 740 750 760 770 780 PPDPSETVST NQPPRPIVKP KRPPPAPPGA VSSSPLPVPV YAPKIPNQFR PDPPTKPLPE 790 800 810 820 LKPKQVKPTF APPTPPVKPG TGGTVPGATQ GAGEPKVALK VPIQKR

References

[1]	Yamamoto S., Yoshiyama K., Setoguchi M., Matsuura K., Higuchi Y., Akizuki S. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ICR.
[2]	"Structure of the murine CD156 gene, characterization of its promoter, and chromosomal location." Kataoka M., Yoshiyama K., Matsuura K., Hijiya N., Higuchi Y., Yamamoto S. J. Biol. Chem. 272:18209-18215(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Liver.
[3]	"Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage." Yoshida S., Setoguchi M., Higuchi Y., Akizuki S., Yamamoto S. Int. Immunol. 2:585-591(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10911 Genomic DNA. Translation: BAA21771.1. X13335 mRNA. Translation: CAA31712.1.
IPI	IPI00114687.
RefSeq	NP_031429.1. NM_007403.2.
UniGene	Mm.15969.
3D structure databases
ProteinModelPortal	Q05910.
SMR	Q05910. Positions 193-644.
ModBase	Search...
Protein family/group databases
MEROPS	M12.208.
PTM databases
PhosphoSite	Q05910.
Proteomic databases
PaxDb	Q05910.
PRIDE	Q05910.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
GeneID	11501.
KEGG	mmu:11501.
UCSC	uc009kgj.1. mouse.
Organism-specific databases
CTD	101.
MGI	MGI:107825. Adam8.
Phylogenomic databases
eggNOG	NOG310718.
HOGENOM	HOG000230883.
HOVERGEN	HBG006978.
KO	K06540.
OMA	NGTPCPG.
Gene expression databases
ArrayExpress	Q05910.
Bgee	Q05910.
CleanEx	MM_ADAM8.
Genevestigator	Q05910.
GermOnline	ENSMUSG00000025473. Mus musculus.
Family and domain databases
Gene3D	3.40.390.10. 1 hit. 4.10.70.10. 1 hit.
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR024079. MetalloPept_cat_dom. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
PRINTS	PR00289. DISINTEGRIN.
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF57552. Disintegrin. 1 hit.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	278902.
PMAP-CutDB	Q05910.
SOURCE	Search...

Entry information

Entry name

ADAM8_MOUSE

Accession

Primary (citable) accession number: Q05910

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program