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Q05910 (ADAM8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 8

Short name=ADAM 8
EC=3.4.24.-
Alternative name(s):
Cell surface antigen MS2
Macrophage cysteine-rich glycoprotein
CD_antigen=CD156a
Gene names
Name:Adam8
Synonyms:Ms2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible involvement in extravasation of leukocytes.

Cofactor

Binds 1 zinc ion per subunit Probable.

Subunit structure

Interacts with FST3 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Macrophages.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity involved in innate immune response

Inferred from direct assay PubMed 20683884. Source: BHF-UCL

angiogenesis

Inferred from direct assay PubMed 19003792. Source: BHF-UCL

blood vessel endothelial cell migration

Non-traceable author statement PubMed 19003792. Source: BHF-UCL

cell morphogenesis

Inferred from mutant phenotype PubMed 11341326PubMed 20683884. Source: BHF-UCL

cell-cell adhesion

Inferred from direct assay PubMed 12372841. Source: MGI

cell-matrix adhesion

Traceable author statement PubMed 11341326. Source: BHF-UCL

cellular response to hypoxia

Inferred from sequence or structural similarity. Source: BHF-UCL

leukocyte migration involved in inflammatory response

Inferred from mutant phenotype PubMed 20194813. Source: BHF-UCL

lymphocyte chemotaxis

Inferred from direct assay PubMed 20856819. Source: BHF-UCL

membrane fusion

Traceable author statement PubMed 11341326. Source: BHF-UCL

negative regulation of neuron apoptotic process

Inferred from direct assay PubMed 14761956. Source: BHF-UCL

negative regulation of thymocyte aggregation

Non-traceable author statement PubMed 20856819. Source: BHF-UCL

osteoclast fusion

Traceable author statement PubMed 12372841. Source: BHF-UCL

positive regulation of NF-kappaB transcription factor activity

Inferred from direct assay PubMed 20683884. Source: BHF-UCL

positive regulation of T cell differentiation in thymus

Inferred from direct assay PubMed 20856819. Source: BHF-UCL

positive regulation of T cell migration

Inferred from mutant phenotype PubMed 20194813. Source: BHF-UCL

positive regulation of acute inflammatory response

Inferred from mutant phenotype PubMed 21268008. Source: BHF-UCL

positive regulation of bone resorption

Inferred from direct assay PubMed 11341326. Source: BHF-UCL

positive regulation of cell adhesion

Inferred from direct assay PubMed 12372841. Source: BHF-UCL

positive regulation of cellular extravasation

Inferred from mutant phenotype PubMed 20194813. Source: BHF-UCL

positive regulation of eosinophil migration

Inferred from mutant phenotype PubMed 20194813. Source: BHF-UCL

positive regulation of fibronectin-dependent thymocyte migration

Inferred from mutant phenotype PubMed 20856819. Source: BHF-UCL

positive regulation of inflammatory response

Inferred from direct assay PubMed 19397475. Source: BHF-UCL

positive regulation of membrane protein ectodomain proteolysis

Inferred from direct assay PubMed 14761956. Source: BHF-UCL

positive regulation of neutrophil extravasation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase B signaling

Inferred from direct assay PubMed 20683884. Source: BHF-UCL

positive regulation of thymocyte apoptotic process

Inferred from mutant phenotype PubMed 20856819. Source: BHF-UCL

positive regulation of tumor necrosis factor (ligand) superfamily member 11 production

Inferred from mutant phenotype PubMed 20683884. Source: BHF-UCL

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cell-cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of osteoclast differentiation

Non-traceable author statement PubMed 18397961. Source: BHF-UCL

tissue morphogenesis

Traceable author statement PubMed 11341326. Source: BHF-UCL

   Cellular_componentalpha9-beta1 integrin-ADAM8 complex

Inferred from direct assay PubMed 16995821. Source: BHF-UCL

alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex

Inferred from electronic annotation. Source: Ensembl

cell surface

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from sequence or structural similarity. Source: BHF-UCL

dense core granule membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

phagolysosome

Inferred from sequence or structural similarity. Source: BHF-UCL

plasma membrane

Inferred from direct assay PubMed 12372841. Source: BHF-UCL

podosome

Inferred from sequence or structural similarity. Source: BHF-UCL

specific granule

Inferred from sequence or structural similarity. Source: BHF-UCL

tertiary granule

Inferred from sequence or structural similarity. Source: BHF-UCL

   Molecular_functioncalcium ion binding

Inferred from direct assay PubMed 12372841. Source: BHF-UCL

cell adhesion molecule binding

Inferred from physical interaction PubMed 12135759PubMed 20826683. Source: BHF-UCL

integrin binding

Traceable author statement PubMed 16831910. Source: BHF-UCL

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

metallopeptidase activity

Inferred from direct assay PubMed 12135759PubMed 14761956. Source: BHF-UCL

zinc ion binding

Inferred from direct assay PubMed 12135759PubMed 12372841. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 826810Disintegrin and metalloproteinase domain-containing protein 8
PRO_0000029061

Regions

Topological domain17 – 658642Extracellular Potential
Transmembrane659 – 68325Helical; Potential
Topological domain684 – 826143Cytoplasmic Potential
Domain196 – 395200Peptidase M12B
Domain403 – 48987Disintegrin
Domain611 – 64333EGF-like

Sites

Active site3301 By similarity
Metal binding3291Zinc; catalytic By similarity
Metal binding3331Zinc; catalytic By similarity
Metal binding3391Zinc; catalytic By similarity

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation2601N-linked (GlcNAc...) Potential
Glycosylation4311N-linked (GlcNAc...) Potential
Glycosylation6141N-linked (GlcNAc...) Potential
Disulfide bond305 ↔ 390 By similarity
Disulfide bond346 ↔ 374 By similarity
Disulfide bond348 ↔ 357 By similarity
Disulfide bond461 ↔ 481 By similarity
Disulfide bond615 ↔ 625 By similarity
Disulfide bond619 ↔ 631 By similarity
Disulfide bond633 ↔ 642 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05910 [UniParc].

Last modified October 1, 1996. Version 3.
Checksum: 3142CC81DBBADFB9

FASTA82690,046
        10         20         30         40         50         60 
MLGLWLLSVL WTPAVAPGPP LPHVKQYEVV WPRRLAASRS RRALPSHWGQ YPESLSYALG 

        70         80         90        100        110        120 
TSGHVFTLHL RKNRDLLGSS YTETYSAANG SEVTEQLQEQ DHCLYQGHVE GYEGSAASIS 

       130        140        150        160        170        180 
TCAGLRGFFR VGSTVHLIEP LDADEEGQHA MYQAKHLQQK AGTCGVKDTN LNDLGPRALE 

       190        200        210        220        230        240 
IYRAQPRNWL IPRETRYVEL YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV 

       250        260        270        280        290        300 
VLVGLEIWNK DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK 

       310        320        330        340        350        360 
VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE PREGGGCIMT 

       370        380        390        400        410        420 
ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF VGGPVCGNLF VEHGEQCDCG 

       430        440        450        460        470        480 
TPQDCQNPCC NATTCQLVKG AECASGTCCH ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT 

       490        500        510        520        530        540 
CPEDAFQQNG TPCPGGYCFD GSCPTLAQQC RDLWGPGARV AADSCYTFSI PPGCNGRMYS 

       550        560        570        580        590        600 
GRINRCGALY CEGGQKPLER SFCTFSSNHG VCHALGTGSN IDTFELVLQG TKCEEGKVCM 

       610        620        630        640        650        660 
DGSCQDLRVY RSENCSAKCN NHGVCNHKRE CHCHKGWAPP NCVQRLADVS DEQAASTSLP 

       670        680        690        700        710        720 
VSVVVVLVIL VAAMVIVAGI VIYRKAPRQI QRRSVAPKPI SGLSNPLFYT RDSSLPAKNR 

       730        740        750        760        770        780 
PPDPSETVST NQPPRPIVKP KRPPPAPPGA VSSSPLPVPV YAPKIPNQFR PDPPTKPLPE 

       790        800        810        820 
LKPKQVKPTF APPTPPVKPG TGGTVPGATQ GAGEPKVALK VPIQKR 

« Hide

References

« Hide 'large scale' references
[1]Yamamoto S., Yoshiyama K., Setoguchi M., Matsuura K., Higuchi Y., Akizuki S.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ICR.
[2]"Structure of the murine CD156 gene, characterization of its promoter, and chromosomal location."
Kataoka M., Yoshiyama K., Matsuura K., Hijiya N., Higuchi Y., Yamamoto S.
J. Biol. Chem. 272:18209-18215(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Liver.
[3]"Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage."
Yoshida S., Setoguchi M., Higuchi Y., Akizuki S., Yamamoto S.
Int. Immunol. 2:585-591(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10911 Genomic DNA. Translation: BAA21771.1.
X13335 mRNA. Translation: CAA31712.1.
RefSeqNP_031429.1. NM_007403.2.
UniGeneMm.15969.

3D structure databases

ProteinModelPortalQ05910.
SMRQ05910. Positions 191-648.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM12.208.

PTM databases

PhosphoSiteQ05910.

Proteomic databases

PaxDbQ05910.
PRIDEQ05910.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000106069; ENSMUSP00000101684; ENSMUSG00000025473.
GeneID11501.
KEGGmmu:11501.
UCSCuc009kgj.1. mouse.

Organism-specific databases

CTD101.
MGIMGI:107825. Adam8.

Phylogenomic databases

eggNOGNOG310718.
HOGENOMHOG000230883.
HOVERGENHBG006978.
KOK06540.
OMANGTPCPG.
PhylomeDBQ05910.
TreeFamTF314733.

Gene expression databases

BgeeQ05910.
CleanExMM_ADAM8.
GenevestigatorQ05910.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF57552. SSF57552. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278902.
PMAP-CutDBQ05910.
PROQ05910.
SOURCESearch...

Entry information

Entry nameADAM8_MOUSE
AccessionPrimary (citable) accession number: Q05910
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot