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Protein

Glutamine synthetase

Gene

glnA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Magnesium 1By similarity1
Metal bindingi124Magnesium 2By similarity1
Binding sitei172ATPBy similarity1
Metal bindingi177Magnesium 2By similarity1
Metal bindingi184Magnesium 2By similarity1
Binding sitei229L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi233Magnesium 1; via pros nitrogenBy similarity1
Binding sitei237ATPBy similarity1
Binding sitei283L-glutamateBy similarity1
Binding sitei289L-glutamateBy similarity1
Binding sitei301ATPBy similarity1
Binding sitei301L-glutamateBy similarity1
Binding sitei306ATPBy similarity1
Binding sitei313ATPBy similarity1
Metal bindingi318Magnesium 1By similarity1
Binding sitei320L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi235 – 237ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:PF0450
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532091 – 439Glutamine synthetaseAdd BLAST439

Proteomic databases

PRIDEiQ05907.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi186497.PF0450.

Structurei

3D structure databases

ProteinModelPortaliQ05907.
SMRiQ05907.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiFWGMNGS.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNISVSMNKF DSKIKFVQLV FVDINGMPKG MEIPASRLEE AVTDGISFDG
60 70 80 90 100
SSVPGFQGIE DSDLVFKADP DTYVEVPWDN VARVYGFIYK DNKPYGADPR
110 120 130 140 150
GILKRALEEL EKEGYKAYIG PEPEFYLFKK NGTWELEIPD VGGYFDILTL
160 170 180 190 200
DKARDIRREI AEYMPSFGLI PEVLHHEVGK AQHEIDFRYD EALKTADNIV
210 220 230 240 250
SFKYITKAVA EMHGLYATFM PKPLFGFPGN GMHLHISLWK DGENVFMGEE
260 270 280 290 300
GLSEIALHFI GGILKHAKAL TAVTNPTVNS YKRLVPSYEA PVYISWGYRN
310 320 330 340 350
RSALIRVPAF WGKGARIEYR CPDPSANPYF AFAAVLKAGL DGIKHKIDPF
360 370 380 390 400
AYVEENVYEM SEEKRKELGI ETLPGSLGEA LEELEKDKVV KEALGDAYKN
410 420 430
FINYKWKEWE SYLEYLEEKH MPKDTKKVTE WELERYFFL
Length:439
Mass (Da):50,183
Last modified:April 3, 2002 - v2
Checksum:i96563A8E9A0E0892
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203K → G in AAA71968 (PubMed:7916055).Curated1
Sequence conflicti232M → L in AAA71968 (PubMed:7916055).Curated1
Sequence conflicti351A → S in AAA71968 (PubMed:7916055).Curated1
Sequence conflicti370I → L in AAA71968 (PubMed:7916055).Curated1
Sequence conflicti376S → N in AAA71968 (PubMed:7916055).Curated1
Sequence conflicti382E → G in AAA71968 (PubMed:7916055).Curated1
Sequence conflicti397A → S in AAA71968 (PubMed:7916055).Curated1
Sequence conflicti421 – 426MPKDTK → IPPDTE in AAA71968 (PubMed:7916055).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12410 Genomic DNA. Translation: AAA71968.1.
AE009950 Genomic DNA. Translation: AAL80574.1.
RefSeqiWP_011011567.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80574; AAL80574; PF0450.
GeneIDi1468291.
KEGGipfu:PF0450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12410 Genomic DNA. Translation: AAA71968.1.
AE009950 Genomic DNA. Translation: AAL80574.1.
RefSeqiWP_011011567.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ05907.
SMRiQ05907.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0450.

Proteomic databases

PRIDEiQ05907.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80574; AAL80574; PF0450.
GeneIDi1468291.
KEGGipfu:PF0450.

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiFWGMNGS.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1A_PYRFU
AccessioniPrimary (citable) accession number: Q05907
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 3, 2002
Last modified: April 12, 2017
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.