Reviewed, UniProtKB/Swiss-Prot Q05901 (ACHB3_HUMAN)

Names and origin

Protein names

Recommended name:
    Neuronal acetylcholine receptor subunit beta-3

Gene names

Name:

CHRNB3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Subunit structure	Neuronal AChR seems to be composed of two different type of subunits: alpha and beta.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ionic channel (TC 1.A.9) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
Molecular function	Ionic channel
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Ref.5 Traceable author statement. Source: ProtInc synaptic transmission, cholinergic Ref.5 Traceable author statement. Source: ProtInc
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW nicotinic acetylcholine-gated receptor-channel complex Ref.5 Traceable author statement. Source: ProtInc postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nicotinic acetylcholine-activated cation-selective channel activity Ref.5 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 458	434	Neuronal acetylcholine receptor subunit beta-3		PRO_0000000383
Regions
	Topological domain	25 – 232	208	Extracellular Potential
	Transmembrane	233 – 257	25	Potential
	Transmembrane	265 – 282	18	Potential
	Transmembrane	299 – 320	22	Potential
	Topological domain	321 – 428	108	Cytoplasmic Potential
	Transmembrane	429 – 447	19	Potential
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Disulfide bond	153 ↔ 167		By similarity
Natural variations
	Natural variant	451	1	K → E: dbSNP rs35327613.		VAR_048173
Experimental info
	Sequence conflict	36 – 38	3	LFQ → EWK in CAA47851. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q05901-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: D6E919E53CBD21F8	FASTA	458	52,729
	10 20 30 40 50 60 MLPDFMLVLI VLGIPSSATT GFNSIAENED ALLRHLFQGY QKWVRPVLHS NDTIKVYFGL 70 80 90 100 110 120 KISQLVDVDE KNQLMTTNVW LKQEWTDHKL RWNPDDYGGI HSIKVPSESL WLPDIVLFEN 130 140 150 160 170 180 ADGRFEGSLM TKVIVKSNGT VVWTPPASYK SSCTMDVTFF PFDRQNCSMK FGSWTYDGTM 190 200 210 220 230 240 VDLILINENV DRKDFFDNGE WEILNAKGMK GNRRDGVYSY PFITYSFVLR RLPLFYTLFL 250 260 270 280 290 300 IIPCLGLSFL TVLVFYLPSD EGEKLSLSTS VLVSLTVFLL VIEEIIPSSS KVIPLIGEYL 310 320 330 340 350 360 LFIMIFVTLS IIVTVFVINV HHRSSSTYHP MAPWVKRLFL QKLPKLLCMK DHVDRYSSPE 370 380 390 400 410 420 KEESQPVVKG KVLEKKKQKQ LSDGEKVLVA FLEKAADSIR YISRHVKKEH FISQVVQDWK 430 440 450 FVAQVLDRIF LWLFLIVSVT GSVLIFTPAL KMWLHSYH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits." Elliott K.J., Ellis S.B., Berckhan K.J., Urrutia A., Chavez-Noriega L.E., Johnson E.C., Velicelebi G., Harpold M.M. J. Mol. Neurosci. 7:217-228(1996) [PubMed: 8906617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Substantia nigra.
[2]	"Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32." Groot Kormelink P.J., Luyten W.H.M.L. FEBS Lett. 400:309-314(1997) [PubMed: 9009220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pons.
[3]	"Genomic structure and mutation analysis of the CHRNB3 gene." Keddache M., Durner M., Greenberg D.A. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Molecular cloning of a human neuronal nicotinic acetylcholine receptor beta 3-like subunit." Willoughby J.J., Ninkina N.N., Beech M.M., Latchman D.S., Wood J.N. Neurosci. Lett. 155:136-139(1993) [PubMed: 7690916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-458. Tissue: Brain stem.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62438 mRNA. Translation: AAB40116.1. Y08417 mRNA. Translation: CAA69694.1. AF140765 , AF140760, AF140761, AF140762, AF140763, AF140764 Genomic DNA. Translation: AAD33063.1. BC069681 mRNA. Translation: AAH69681.1. BC069703 mRNA. Translation: AAH69703.1. BC069788 mRNA. Translation: AAH69788.1. X67513 mRNA. Translation: CAA47851.1.
IPI	IPI00016662.
PIR	I38056.
RefSeq	NP_000740.1.
UniGene	Hs.654576
3D structure databases
SMR	Q05901. Positions 28-450.
ModBase	Search...
Protein-protein interaction databases
STRING	Q05901.
Proteomic databases
PRIDE	Q05901.
Genome annotation databases
Ensembl	ENST00000289957; ENSP00000289957; ENSG00000147432; Homo sapiens. [Genome view]
GeneID	1142.
KEGG	hsa:1142.
UCSC	uc003xpi.1. human.
Organism-specific databases
CTD	1142.
GeneCards	GC08P042671.
H-InvDB	HIX0034355.
HGNC	HGNC:1963. CHRNB3.
MIM	118508. gene.
PharmGKB	PA26495.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05964.
HOGENOM	HBG387619.
HOVERGEN	Q05901.
InParanoid	Q05901.
OMA	FITYSFV.
OrthoDB	EOG9XH2JJ.
PhylomeDB	Q05901.
Gene expression databases
ArrayExpress	Q05901.
Bgee	Q05901.
CleanEx	HS_CHRNB3.
Genevestigator	Q05901.
GermOnline	ENSG00000147432. Homo sapiens.
Family and domain databases
InterPro	IPR006202. Neur_chan_lig_bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt_N. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	4752.
SOURCE	Search...

Entry information

Entry name

ACHB3_HUMAN

Accession

Primary (citable) accession number: Q05901
Secondary accession number(s): Q15827

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.