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Protein

Neuronal acetylcholine receptor subunit beta-3

Gene

CHRNB3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  1. acetylcholine-activated cation-selective channel activity Source: ProtInc
  2. acetylcholine binding Source: Ensembl
  3. channel activity Source: ProtInc
  4. drug binding Source: Ensembl

GO - Biological processi

  1. cation transmembrane transport Source: GOC
  2. cation transport Source: GOC
  3. protein heterooligomerization Source: Ensembl
  4. signal transduction Source: ProtInc
  5. synaptic transmission Source: Reactome
  6. synaptic transmission, cholinergic Source: ProtInc
  7. transmembrane transport Source: GOC
  8. transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_22303. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_22352. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-3
Gene namesi
Name:CHRNB3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:1963. CHRNB3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 232208ExtracellularSequence AnalysisAdd
BLAST
Transmembranei233 – 25725HelicalSequence AnalysisAdd
BLAST
Transmembranei265 – 28218HelicalSequence AnalysisAdd
BLAST
Transmembranei299 – 32022HelicalSequence AnalysisAdd
BLAST
Topological domaini321 – 428108CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei429 – 44719HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. acetylcholine-gated channel complex Source: ProtInc
  2. cell junction Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB
  4. neuron projection Source: Ensembl
  5. plasma membrane Source: Reactome
  6. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26495.

Chemistry

DrugBankiDB00674. Galantamine.
DB00184. Nicotine.

Polymorphism and mutation databases

BioMutaiCHRNB3.
DMDMi2506128.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 458434Neuronal acetylcholine receptor subunit beta-3PRO_0000000383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi153 ↔ 167By similarity
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ05901.
PaxDbiQ05901.
PRIDEiQ05901.

PTM databases

PhosphoSiteiQ05901.

Expressioni

Gene expression databases

BgeeiQ05901.
CleanExiHS_CHRNB3.
GenevestigatoriQ05901.

Organism-specific databases

HPAiHPA045555.

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different type of subunits: alpha and beta.

Protein-protein interaction databases

BioGridi107564. 2 interactions.
STRINGi9606.ENSP00000289957.

Structurei

3D structure databases

ProteinModelPortaliQ05901.
SMRiQ05901. Positions 26-340, 395-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG252943.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ05901.
KOiK04814.
OMAiDRYSFPD.
PhylomeDBiQ05901.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05901-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPDFMLVLI VLGIPSSATT GFNSIAENED ALLRHLFQGY QKWVRPVLHS
60 70 80 90 100
NDTIKVYFGL KISQLVDVDE KNQLMTTNVW LKQEWTDHKL RWNPDDYGGI
110 120 130 140 150
HSIKVPSESL WLPDIVLFEN ADGRFEGSLM TKVIVKSNGT VVWTPPASYK
160 170 180 190 200
SSCTMDVTFF PFDRQNCSMK FGSWTYDGTM VDLILINENV DRKDFFDNGE
210 220 230 240 250
WEILNAKGMK GNRRDGVYSY PFITYSFVLR RLPLFYTLFL IIPCLGLSFL
260 270 280 290 300
TVLVFYLPSD EGEKLSLSTS VLVSLTVFLL VIEEIIPSSS KVIPLIGEYL
310 320 330 340 350
LFIMIFVTLS IIVTVFVINV HHRSSSTYHP MAPWVKRLFL QKLPKLLCMK
360 370 380 390 400
DHVDRYSSPE KEESQPVVKG KVLEKKKQKQ LSDGEKVLVA FLEKAADSIR
410 420 430 440 450
YISRHVKKEH FISQVVQDWK FVAQVLDRIF LWLFLIVSVT GSVLIFTPAL

KMWLHSYH
Length:458
Mass (Da):52,729
Last modified:November 1, 1997 - v2
Checksum:iD6E919E53CBD21F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 383LFQ → EWK in CAA47851 (PubMed:7690916).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti451 – 4511K → E.
Corresponds to variant rs35327613 [ dbSNP | Ensembl ].
VAR_048173

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62438 mRNA. Translation: AAB40116.1.
Y08417 mRNA. Translation: CAA69694.1.
AF140765
, AF140760, AF140761, AF140762, AF140763, AF140764 Genomic DNA. Translation: AAD33063.1.
BC069681 mRNA. Translation: AAH69681.1.
BC069703 mRNA. Translation: AAH69703.1.
BC069788 mRNA. Translation: AAH69788.1.
X67513 mRNA. Translation: CAA47851.1.
CCDSiCCDS6134.1.
PIRiI38056.
RefSeqiNP_000740.1. NM_000749.3.
UniGeneiHs.654576.

Genome annotation databases

EnsembliENST00000289957; ENSP00000289957; ENSG00000147432.
GeneIDi1142.
KEGGihsa:1142.
UCSCiuc003xpi.1. human.

Polymorphism and mutation databases

BioMutaiCHRNB3.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62438 mRNA. Translation: AAB40116.1.
Y08417 mRNA. Translation: CAA69694.1.
AF140765
, AF140760, AF140761, AF140762, AF140763, AF140764 Genomic DNA. Translation: AAD33063.1.
BC069681 mRNA. Translation: AAH69681.1.
BC069703 mRNA. Translation: AAH69703.1.
BC069788 mRNA. Translation: AAH69788.1.
X67513 mRNA. Translation: CAA47851.1.
CCDSiCCDS6134.1.
PIRiI38056.
RefSeqiNP_000740.1. NM_000749.3.
UniGeneiHs.654576.

3D structure databases

ProteinModelPortaliQ05901.
SMRiQ05901. Positions 26-340, 395-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107564. 2 interactions.
STRINGi9606.ENSP00000289957.

Chemistry

BindingDBiQ05901.
ChEMBLiCHEMBL2109233.
DrugBankiDB00674. Galantamine.
DB00184. Nicotine.
GuidetoPHARMACOLOGYi473.

PTM databases

PhosphoSiteiQ05901.

Polymorphism and mutation databases

BioMutaiCHRNB3.
DMDMi2506128.

Proteomic databases

MaxQBiQ05901.
PaxDbiQ05901.
PRIDEiQ05901.

Protocols and materials databases

DNASUi1142.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000289957; ENSP00000289957; ENSG00000147432.
GeneIDi1142.
KEGGihsa:1142.
UCSCiuc003xpi.1. human.

Organism-specific databases

CTDi1142.
GeneCardsiGC08P042552.
HGNCiHGNC:1963. CHRNB3.
HPAiHPA045555.
MIMi118508. gene.
neXtProtiNX_Q05901.
PharmGKBiPA26495.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG252943.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ05901.
KOiK04814.
OMAiDRYSFPD.
PhylomeDBiQ05901.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiREACT_22303. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_22352. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

ChiTaRSiCHRNB3. human.
GeneWikiiCHRNB3.
GenomeRNAii1142.
NextBioi4752.
PROiQ05901.
SOURCEiSearch...

Gene expression databases

BgeeiQ05901.
CleanExiHS_CHRNB3.
GenevestigatoriQ05901.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits."
    Elliott K.J., Ellis S.B., Berckhan K.J., Urrutia A., Chavez-Noriega L.E., Johnson E.C., Velicelebi G., Harpold M.M.
    J. Mol. Neurosci. 7:217-228(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Substantia nigra.
  2. "Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32."
    Groot Kormelink P.J., Luyten W.H.M.L.
    FEBS Lett. 400:309-314(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pons.
  3. "Genomic structure and mutation analysis of the CHRNB3 gene."
    Keddache M., Durner M., Greenberg D.A.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Molecular cloning of a human neuronal nicotinic acetylcholine receptor beta 3-like subunit."
    Willoughby J.J., Ninkina N.N., Beech M.M., Latchman D.S., Wood J.N.
    Neurosci. Lett. 155:136-139(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-458.
    Tissue: Brain stem.

Entry informationi

Entry nameiACHB3_HUMAN
AccessioniPrimary (citable) accession number: Q05901
Secondary accession number(s): Q15827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: April 29, 2015
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.