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Q05901

- ACHB3_HUMAN

UniProt

Q05901 - ACHB3_HUMAN

Protein

Neuronal acetylcholine receptor subunit beta-3

Gene

CHRNB3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

    GO - Molecular functioni

    1. acetylcholine-activated cation-selective channel activity Source: ProtInc
    2. acetylcholine binding Source: Ensembl
    3. channel activity Source: ProtInc
    4. drug binding Source: Ensembl

    GO - Biological processi

    1. cation transport Source: GOC
    2. protein heterooligomerization Source: Ensembl
    3. signal transduction Source: ProtInc
    4. synaptic transmission Source: Reactome
    5. synaptic transmission, cholinergic Source: ProtInc
    6. transmembrane transport Source: GOC
    7. transport Source: ProtInc

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_22303. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_22352. Highly calcium permeable nicotinic acetylcholine receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuronal acetylcholine receptor subunit beta-3
    Gene namesi
    Name:CHRNB3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:1963. CHRNB3.

    Subcellular locationi

    GO - Cellular componenti

    1. acetylcholine-gated channel complex Source: ProtInc
    2. cell junction Source: UniProtKB-KW
    3. integral component of membrane Source: UniProtKB
    4. neuron projection Source: Ensembl
    5. plasma membrane Source: Reactome
    6. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26495.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 458434Neuronal acetylcholine receptor subunit beta-3PRO_0000000383Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi153 ↔ 167By similarity
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ05901.
    PaxDbiQ05901.
    PRIDEiQ05901.

    PTM databases

    PhosphoSiteiQ05901.

    Expressioni

    Gene expression databases

    BgeeiQ05901.
    CleanExiHS_CHRNB3.
    GenevestigatoriQ05901.

    Organism-specific databases

    HPAiHPA045555.

    Interactioni

    Subunit structurei

    Neuronal AChR seems to be composed of two different type of subunits: alpha and beta.

    Protein-protein interaction databases

    STRINGi9606.ENSP00000289957.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05901.
    SMRiQ05901. Positions 26-340, 395-450.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 232208ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini321 – 428108CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei233 – 25725HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28218HelicalSequence AnalysisAdd
    BLAST
    Transmembranei299 – 32022HelicalSequence AnalysisAdd
    BLAST
    Transmembranei429 – 44719HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG252943.
    HOGENOMiHOG000006756.
    HOVERGENiHBG003756.
    InParanoidiQ05901.
    KOiK04814.
    OMAiDRYSFPD.
    PhylomeDBiQ05901.
    TreeFamiTF315605.

    Family and domain databases

    Gene3Di1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProiIPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05901-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPDFMLVLI VLGIPSSATT GFNSIAENED ALLRHLFQGY QKWVRPVLHS    50
    NDTIKVYFGL KISQLVDVDE KNQLMTTNVW LKQEWTDHKL RWNPDDYGGI 100
    HSIKVPSESL WLPDIVLFEN ADGRFEGSLM TKVIVKSNGT VVWTPPASYK 150
    SSCTMDVTFF PFDRQNCSMK FGSWTYDGTM VDLILINENV DRKDFFDNGE 200
    WEILNAKGMK GNRRDGVYSY PFITYSFVLR RLPLFYTLFL IIPCLGLSFL 250
    TVLVFYLPSD EGEKLSLSTS VLVSLTVFLL VIEEIIPSSS KVIPLIGEYL 300
    LFIMIFVTLS IIVTVFVINV HHRSSSTYHP MAPWVKRLFL QKLPKLLCMK 350
    DHVDRYSSPE KEESQPVVKG KVLEKKKQKQ LSDGEKVLVA FLEKAADSIR 400
    YISRHVKKEH FISQVVQDWK FVAQVLDRIF LWLFLIVSVT GSVLIFTPAL 450
    KMWLHSYH 458
    Length:458
    Mass (Da):52,729
    Last modified:November 1, 1997 - v2
    Checksum:iD6E919E53CBD21F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 383LFQ → EWK in CAA47851. (PubMed:7690916)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti451 – 4511K → E.
    Corresponds to variant rs35327613 [ dbSNP | Ensembl ].
    VAR_048173

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62438 mRNA. Translation: AAB40116.1.
    Y08417 mRNA. Translation: CAA69694.1.
    AF140765
    , AF140760, AF140761, AF140762, AF140763, AF140764 Genomic DNA. Translation: AAD33063.1.
    BC069681 mRNA. Translation: AAH69681.1.
    BC069703 mRNA. Translation: AAH69703.1.
    BC069788 mRNA. Translation: AAH69788.1.
    X67513 mRNA. Translation: CAA47851.1.
    CCDSiCCDS6134.1.
    PIRiI38056.
    RefSeqiNP_000740.1. NM_000749.3.
    UniGeneiHs.654576.

    Genome annotation databases

    EnsembliENST00000289957; ENSP00000289957; ENSG00000147432.
    GeneIDi1142.
    KEGGihsa:1142.
    UCSCiuc003xpi.1. human.

    Polymorphism databases

    DMDMi2506128.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62438 mRNA. Translation: AAB40116.1 .
    Y08417 mRNA. Translation: CAA69694.1 .
    AF140765
    , AF140760 , AF140761 , AF140762 , AF140763 , AF140764 Genomic DNA. Translation: AAD33063.1 .
    BC069681 mRNA. Translation: AAH69681.1 .
    BC069703 mRNA. Translation: AAH69703.1 .
    BC069788 mRNA. Translation: AAH69788.1 .
    X67513 mRNA. Translation: CAA47851.1 .
    CCDSi CCDS6134.1.
    PIRi I38056.
    RefSeqi NP_000740.1. NM_000749.3.
    UniGenei Hs.654576.

    3D structure databases

    ProteinModelPortali Q05901.
    SMRi Q05901. Positions 26-340, 395-450.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000289957.

    Chemistry

    BindingDBi Q05901.
    ChEMBLi CHEMBL2109233.
    DrugBanki DB00674. Galantamine.
    DB00184. Nicotine.
    GuidetoPHARMACOLOGYi 473.

    PTM databases

    PhosphoSitei Q05901.

    Polymorphism databases

    DMDMi 2506128.

    Proteomic databases

    MaxQBi Q05901.
    PaxDbi Q05901.
    PRIDEi Q05901.

    Protocols and materials databases

    DNASUi 1142.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000289957 ; ENSP00000289957 ; ENSG00000147432 .
    GeneIDi 1142.
    KEGGi hsa:1142.
    UCSCi uc003xpi.1. human.

    Organism-specific databases

    CTDi 1142.
    GeneCardsi GC08P042552.
    HGNCi HGNC:1963. CHRNB3.
    HPAi HPA045555.
    MIMi 118508. gene.
    neXtProti NX_Q05901.
    PharmGKBi PA26495.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG252943.
    HOGENOMi HOG000006756.
    HOVERGENi HBG003756.
    InParanoidi Q05901.
    KOi K04814.
    OMAi DRYSFPD.
    PhylomeDBi Q05901.
    TreeFami TF315605.

    Enzyme and pathway databases

    Reactomei REACT_22303. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_22352. Highly calcium permeable nicotinic acetylcholine receptors.

    Miscellaneous databases

    GeneWikii CHRNB3.
    GenomeRNAii 1142.
    NextBioi 4752.
    PROi Q05901.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q05901.
    CleanExi HS_CHRNB3.
    Genevestigatori Q05901.

    Family and domain databases

    Gene3Di 1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProi IPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits."
      Elliott K.J., Ellis S.B., Berckhan K.J., Urrutia A., Chavez-Noriega L.E., Johnson E.C., Velicelebi G., Harpold M.M.
      J. Mol. Neurosci. 7:217-228(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Substantia nigra.
    2. "Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32."
      Groot Kormelink P.J., Luyten W.H.M.L.
      FEBS Lett. 400:309-314(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pons.
    3. "Genomic structure and mutation analysis of the CHRNB3 gene."
      Keddache M., Durner M., Greenberg D.A.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Molecular cloning of a human neuronal nicotinic acetylcholine receptor beta 3-like subunit."
      Willoughby J.J., Ninkina N.N., Beech M.M., Latchman D.S., Wood J.N.
      Neurosci. Lett. 155:136-139(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-458.
      Tissue: Brain stem.

    Entry informationi

    Entry nameiACHB3_HUMAN
    AccessioniPrimary (citable) accession number: Q05901
    Secondary accession number(s): Q15827
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3