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Q05901 (ACHB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit beta-3
Gene names
Name:CHRNB3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure

Neuronal AChR seems to be composed of two different type of subunits: alpha and beta.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-3/CHRNB3 sub-subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcation transport

Traceable author statement Ref.5. Source: GOC

protein heterooligomerization

Inferred from electronic annotation. Source: Ensembl

signal transduction

Traceable author statement Ref.5. Source: ProtInc

synaptic transmission

Traceable author statement. Source: Reactome

synaptic transmission, cholinergic

Traceable author statement Ref.5. Source: ProtInc

transmembrane transport

Traceable author statement Ref.5. Source: GOC

transport

Traceable author statement Ref.5. Source: ProtInc

   Cellular_componentacetylcholine-gated channel complex

Traceable author statement Ref.5. Source: ProtInc

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Non-traceable author statement Ref.1. Source: UniProtKB

neuron projection

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacetylcholine binding

Inferred from electronic annotation. Source: Ensembl

acetylcholine-activated cation-selective channel activity

Traceable author statement Ref.5. Source: ProtInc

channel activity

Traceable author statement Ref.5. Source: ProtInc

drug binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 458434Neuronal acetylcholine receptor subunit beta-3
PRO_0000000383

Regions

Topological domain25 – 232208Extracellular Potential
Transmembrane233 – 25725Helical; Potential
Transmembrane265 – 28218Helical; Potential
Transmembrane299 – 32022Helical; Potential
Topological domain321 – 428108Cytoplasmic Potential
Transmembrane429 – 44719Helical; Potential

Amino acid modifications

Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Disulfide bond153 ↔ 167 By similarity

Natural variations

Natural variant4511K → E.
Corresponds to variant rs35327613 [ dbSNP | Ensembl ].
VAR_048173

Experimental info

Sequence conflict36 – 383LFQ → EWK in CAA47851. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q05901 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: D6E919E53CBD21F8

FASTA45852,729
        10         20         30         40         50         60 
MLPDFMLVLI VLGIPSSATT GFNSIAENED ALLRHLFQGY QKWVRPVLHS NDTIKVYFGL 

        70         80         90        100        110        120 
KISQLVDVDE KNQLMTTNVW LKQEWTDHKL RWNPDDYGGI HSIKVPSESL WLPDIVLFEN 

       130        140        150        160        170        180 
ADGRFEGSLM TKVIVKSNGT VVWTPPASYK SSCTMDVTFF PFDRQNCSMK FGSWTYDGTM 

       190        200        210        220        230        240 
VDLILINENV DRKDFFDNGE WEILNAKGMK GNRRDGVYSY PFITYSFVLR RLPLFYTLFL 

       250        260        270        280        290        300 
IIPCLGLSFL TVLVFYLPSD EGEKLSLSTS VLVSLTVFLL VIEEIIPSSS KVIPLIGEYL 

       310        320        330        340        350        360 
LFIMIFVTLS IIVTVFVINV HHRSSSTYHP MAPWVKRLFL QKLPKLLCMK DHVDRYSSPE 

       370        380        390        400        410        420 
KEESQPVVKG KVLEKKKQKQ LSDGEKVLVA FLEKAADSIR YISRHVKKEH FISQVVQDWK 

       430        440        450 
FVAQVLDRIF LWLFLIVSVT GSVLIFTPAL KMWLHSYH 

« Hide

References

« Hide 'large scale' references
[1]"Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits."
Elliott K.J., Ellis S.B., Berckhan K.J., Urrutia A., Chavez-Noriega L.E., Johnson E.C., Velicelebi G., Harpold M.M.
J. Mol. Neurosci. 7:217-228(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Substantia nigra.
[2]"Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32."
Groot Kormelink P.J., Luyten W.H.M.L.
FEBS Lett. 400:309-314(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pons.
[3]"Genomic structure and mutation analysis of the CHRNB3 gene."
Keddache M., Durner M., Greenberg D.A.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Molecular cloning of a human neuronal nicotinic acetylcholine receptor beta 3-like subunit."
Willoughby J.J., Ninkina N.N., Beech M.M., Latchman D.S., Wood J.N.
Neurosci. Lett. 155:136-139(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-458.
Tissue: Brain stem.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U62438 mRNA. Translation: AAB40116.1.
Y08417 mRNA. Translation: CAA69694.1.
AF140765 expand/collapse EMBL AC list , AF140760, AF140761, AF140762, AF140763, AF140764 Genomic DNA. Translation: AAD33063.1.
BC069681 mRNA. Translation: AAH69681.1.
BC069703 mRNA. Translation: AAH69703.1.
BC069788 mRNA. Translation: AAH69788.1.
X67513 mRNA. Translation: CAA47851.1.
CCDSCCDS6134.1.
PIRI38056.
RefSeqNP_000740.1. NM_000749.3.
UniGeneHs.654576.

3D structure databases

ProteinModelPortalQ05901.
SMRQ05901. Positions 26-340, 395-450.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000289957.

Chemistry

BindingDBQ05901.
ChEMBLCHEMBL2109233.
GuidetoPHARMACOLOGY473.

PTM databases

PhosphoSiteQ05901.

Polymorphism databases

DMDM2506128.

Proteomic databases

MaxQBQ05901.
PaxDbQ05901.
PRIDEQ05901.

Protocols and materials databases

DNASU1142.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000289957; ENSP00000289957; ENSG00000147432.
GeneID1142.
KEGGhsa:1142.
UCSCuc003xpi.1. human.

Organism-specific databases

CTD1142.
GeneCardsGC08P042552.
HGNCHGNC:1963. CHRNB3.
HPAHPA045555.
MIM118508. gene.
neXtProtNX_Q05901.
PharmGKBPA26495.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG252943.
HOGENOMHOG000006756.
HOVERGENHBG003756.
InParanoidQ05901.
KOK04814.
OMADRYSFPD.
PhylomeDBQ05901.
TreeFamTF315605.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

BgeeQ05901.
CleanExHS_CHRNB3.
GenevestigatorQ05901.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCHRNB3.
GenomeRNAi1142.
NextBio4752.
PROQ05901.
SOURCESearch...

Entry information

Entry nameACHB3_HUMAN
AccessionPrimary (citable) accession number: Q05901
Secondary accession number(s): Q15827
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM