Reviewed, UniProtKB/Swiss-Prot Q058E7 (METE_BUCCC)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BCc_017

Organism

Buchnera aphidicola subsp. Cinara cedri [Complete proteome] [HAMAP]

Taxonomic identifier

372461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017228
Sites
	Metal binding	644	1	Zinc By similarity
	Metal binding	646	1	Zinc By similarity
	Metal binding	729	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q058E7-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 8BC2EFDEBB51F8AD	FASTA	760	88,414
	10 20 30 40 50 60 MAIKNHILGF PRIGLNRELK FALEKYWSKK NTLEELLLIG KNIRKENWKN QIDSGMDYVT 70 80 90 100 110 120 VGDFAWYDHV LNISMMINNI PERHNPNNHI LNIDTLFKVA RGSKGVDNNI CSASEMTKWF 130 140 150 160 170 180 NTNYHYIVPE FTSNQKFYFA WKQILEETDE ALSLGYKVKP VLLGPLTYLW LGKVKNSKIN 190 200 210 220 230 240 KLDLLKKILP IYIQVFKELS SRNINWIQID EPILVLDIPK NWKKEFQSTY KFLDGKIKIL 250 260 270 280 290 300 LATYFGDITH NLDIINKLSI QGLHIDLVSS KYDLLKLSKS INNNFLLSLG IINGRNIWKT 310 320 330 340 350 360 NLLEWFYKLK DFMKINNNFW ISSSCSLLHV PLDITIEENL TDFVKSWFSF GIQKCLEISL 370 380 390 400 410 420 LSQVLQNNLD IKELKNWIKP IHEYKSSNIV NNTSVQKRTL KISSEQFIRK NEFSVRSKIQ 430 440 450 460 470 480 KETLCLPVLP TTTIGSFPQT SEIRKLRLDY KNKKINQLDY EKQIKIHIKK NIIQQEQLGL 490 500 510 520 530 540 DVLVHGEPER NDMVEYFSEY LEGFVFTTYG WVQSYGSRCV KPPIIVGDIS RITPMTVMWS 550 560 570 580 590 600 KYAQSLTKKP VKAMLTGPVT ILCWSFPRED ISKEDICNQI AISLRDEVLD LENSGINIIQ 610 620 630 640 650 660 IDEPALREGL PLRTHEWNNY LRWAVKSFKI CSSGVKNSTQ IHTHMCYCEF NDIMPAIVDL 670 680 690 700 710 720 DADVITIETS RSDMELLEFF KTFKYPNAIG PGVYDIHSPN IPSVQSIEKL LKKALKYISI 730 740 750 760 QQLWVNPDCG LKTRNWTETS LALQNMLQAT LNIRKEYFKK

References

[1]	"A small microbial genome: the end of a long symbiotic relationship?" Perez-Brocal V., Gil R., Ramos S., Lamelas A., Postigo M., Michelena J.M., Silva F.J., Moya A., Latorre A. Science 314:312-313(2006) [PubMed: 17038625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000263 Genomic DNA. Translation: ABJ90502.1.
RefSeq	YP_802595.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q058E7.
Genome annotation databases
GeneID	4440657.
GenomeReviews	Gene locus BCc_017 in contig CP000263_GR.
KEGG	bcc:BCc_017.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BUCCC

Accession

Primary (citable) accession number: Q058E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)