ID   ASSY_BUCCC              Reviewed;         399 AA.
AC   Q058D6;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=Argininosuccinate synthase;
DE            EC=6.3.4.5;
DE   AltName: Full=Citrulline--aspartate ligase;
GN   Name=argG; OrderedLocusNames=BCc_032;
OS   Buchnera aphidicola subsp. Cinara cedri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=372461;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17038625; DOI=10.1126/science.1130441;
RA   Perez-Brocal V., Gil R., Ramos S., Lamelas A., Postigo M.,
RA   Michelena J.M., Silva F.J., Moya A., Latorre A.;
RT   "A small microbial genome: the end of a long symbiotic relationship?";
RL   Science 314:312-313(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC       diphosphate + N(omega)-(L-arginino)succinate.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC       arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC       1 subfamily.
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DR   EMBL; CP000263; ABJ90513.1; -; Genomic_DNA.
DR   RefSeq; YP_802606.1; -.
DR   GeneID; 4440707; -.
DR   GenomeReviews; CP000263_GR; BCc_032.
DR   KEGG; bcc:BCc_032; -.
DR   OMA; Q058D6; YVFPMFR.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00005; -; 1.
DR   InterPro; IPR001518; Arginosuc_synth.
DR   InterPro; IPR018223; Arginosuc_synth_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR11587; Arginosuc_synth; 1.
DR   Pfam; PF00764; Arginosuc_synth; 1.
DR   TIGRFAMs; TIGR00032; argG; 1.
DR   PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR   PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW   Complete proteome; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN         1    399       Argininosuccinate synthase.
FT                                /FTId=PRO_1000057038.
FT   NP_BIND       8     16       ATP (By similarity).
FT   BINDING      35     35       ATP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   BINDING      87     87       Citrulline (By similarity).
FT   BINDING     117    117       ATP; via amide nitrogen (By similarity).
FT   BINDING     119    119       Aspartate (By similarity).
FT   BINDING     123    123       Aspartate (By similarity).
FT   BINDING     123    123       Citrulline (By similarity).
FT   BINDING     124    124       Aspartate (By similarity).
FT   BINDING     127    127       Citrulline (By similarity).
FT   BINDING     176    176       Citrulline (By similarity).
FT   BINDING     185    185       Citrulline (By similarity).
FT   BINDING     261    261       Citrulline (By similarity).
FT   BINDING     273    273       Citrulline (By similarity).
SQ   SEQUENCE   399 AA;  45285 MW;  65E279640523F661 CRC64;
     MKKTIVLAYS GGLDTSAIIP WIKDNYNFDV VAFVADIGQS KKDLYKIKEK AIISGASDCY
     ISDLKDIFVK KYVFPMLKTG AIYEGEYLLG TAIARPLIAK AQVDYAKKIN AIGLCHGSTG
     KGNDQVRFEL AYSALAPSLL VIAPWREWKF QSREDLLKYL KTKNIVTNVN KKKIYSRDEN
     IFHVSTEGGI LEDPWNPANE DCWFWTKSPL NAPNKPKKIS LKIEKGCVVS INNKFFNEFN
     CLKRLNKIGA KHSIGRIDIV ENRLIGMKSR GCYETPGGTI IYKALRSLEQ LVFDRECMYW
     KNKIALQLSS IIYDGKWFTP IRKSLQKSSD ILSSSISGKV VVELYKGSVR ILQKKSLNSL
     YSKKYVTFGK DNVYNQIDAK GFIRLFSLSS RIRALKNKK
//