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Protein

Thrombospondin-3

Gene

Thbs3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen.

GO - Molecular functioni

GO - Biological processi

  • bone trabecula formation Source: MGI
  • cell adhesion Source: UniProtKB-KW
  • growth plate cartilage development Source: MGI
  • ossification involved in bone maturation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-186797. Signaling by PDGF.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-3
Gene namesi
Name:Thbs3
Synonyms:Tsp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:98739. Thbs3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000003585022 – 956Thrombospondin-3Add BLAST935

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi266InterchainCurated
Disulfide bondi269InterchainCurated
Disulfide bondi278 ↔ 289PROSITE-ProRule annotation
Disulfide bondi283 ↔ 300PROSITE-ProRule annotation
Disulfide bondi303 ↔ 314PROSITE-ProRule annotation
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi320 ↔ 332PROSITE-ProRule annotation
Disulfide bondi326 ↔ 341PROSITE-ProRule annotation
Disulfide bondi344 ↔ 368PROSITE-ProRule annotation
Disulfide bondi374 ↔ 388PROSITE-ProRule annotation
Disulfide bondi382 ↔ 397PROSITE-ProRule annotation
Disulfide bondi400 ↔ 412PROSITE-ProRule annotation
Glycosylationi407N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi418 ↔ 432PROSITE-ProRule annotation
Disulfide bondi426 ↔ 442PROSITE-ProRule annotation
Disulfide bondi444 ↔ 455PROSITE-ProRule annotation
Disulfide bondi471 ↔ 478PROSITE-ProRule annotation
Disulfide bondi483 ↔ 503PROSITE-ProRule annotation
Disulfide bondi519 ↔ 539PROSITE-ProRule annotation
Disulfide bondi542 ↔ 562PROSITE-ProRule annotation
Disulfide bondi578 ↔ 598PROSITE-ProRule annotation
Disulfide bondi601 ↔ 621PROSITE-ProRule annotation
Disulfide bondi639 ↔ 659PROSITE-ProRule annotation
Glycosylationi644N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi679 ↔ 699PROSITE-ProRule annotation
Disulfide bondi715 ↔ 936PROSITE-ProRule annotation
Glycosylationi937N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ05895.
PaxDbiQ05895.
PRIDEiQ05895.

PTM databases

PhosphoSitePlusiQ05895.

Expressioni

Tissue specificityi

Brain, lung and cartilage.

Gene expression databases

BgeeiENSMUSG00000028047.
CleanExiMM_THBS3.
ExpressionAtlasiQ05895. baseline and differential.
GenevisibleiQ05895. MM.

Interactioni

Subunit structurei

Oligomer; disulfide-linked.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029682.

Structurei

3D structure databases

ProteinModelPortaliQ05895.
SMRiQ05895.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 193Laminin G-likeAdd BLAST172
Domaini316 – 354EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini370 – 410EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini414 – 456EGF-like 3PROSITE-ProRule annotationAdd BLAST43
Repeati457 – 491TSP type-3 1Add BLAST35
Repeati492 – 527TSP type-3 2Add BLAST36
Repeati528 – 550TSP type-3 3Add BLAST23
Repeati551 – 586TSP type-3 4Add BLAST36
Repeati587 – 609TSP type-3 5Add BLAST23
Repeati610 – 647TSP type-3 6Add BLAST38
Repeati648 – 687TSP type-3 7Add BLAST40
Repeati688 – 723TSP type-3 8Add BLAST36
Domaini727 – 941TSP C-terminalPROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
GeneTreeiENSGT00850000132273.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiQ05895.
KOiK04659.
OMAiYQDSGRF.
OrthoDBiEOG091G00TV.
TreeFamiTF324917.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR028507. Thrombospondin-3.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PANTHERiPTHR10199:SF89. PTHR10199:SF89. 1 hit.
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPELWGVL ALLLLCSYTC GSQDLQVIDL LTVGESRQMV AVAEKIRTAL
60 70 80 90 100
LTAGDIYLLS TFRLPPKQGG VLFGLYSRQD NTRWLEASVV GKINKVLVRY
110 120 130 140 150
QREDGKVHAV NLQQAGLADG RTHTALLRLR GPSRPSPGLQ LYVDCKLGDQ
160 170 180 190 200
HAGLPALAPI PPAEVSGLEI RTGQKAYLRM QGFVESMKII LGGSMARVGA
210 220 230 240 250
LSECPFQGDD SIHNAVTSAL QSILGEQTKA LVTQLTLFNQ ILVELRDDIR
260 270 280 290 300
DQVKEMSLIR NTIMECQVCG FHEQRSHCSP SPCFRGVDCM EVYEYPGYRC
310 320 330 340 350
GPCPPGLQGN GTHCDDINEC AHADPCFPGS SCINTMPGFH CEACPPGYKG
360 370 380 390 400
TRVSGVGIDY ARASKQVCND IDECNDGNNG GCDPNSICTN TVGSFKCGPC
410 420 430 440 450
RLGFLGNQSQ GCVPARTCHS PAHSPCHIHA HCLFERNGAV SCQCNVGWAG
460 470 480 490 500
NGNVCGPDTD IDGYPDQALP CMDNNKHCKQ DNCLLTPNSG QEDADNDGVG
510 520 530 540 550
DQCDDDADGD GIKNVEDNCR LFPNKDQQNS DTDSFGDACD NCPNVPNNDQ
560 570 580 590 600
KDTDGNGEGD ACDNDVDGDG IPNGLDNCPK VPNPLQTDRD EDGVGDACDS
610 620 630 640 650
CPEMSNPTQT DADSDLVGDV CDTNEDSDGD GHQDTKDNCP QLPNSSQLDS
660 670 680 690 700
DNDGLGDECD GDDDNDGVPD YIPPGPDNCR LVPNPNQKDS DGNGVGDVCE
710 720 730 740 750
DDFDNDAVVD PLDVCPESAE VTLTDFRAYQ TVILDPEGDA QIDPNWVVLN
760 770 780 790 800
QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTVTDDD YAGFLFSYQD
810 820 830 840 850
SGRFYVVMWK QTEQTYWQAT PFRAVAQPGL QLKAVTSISG PGEHLRNALW
860 870 880 890 900
HTGHTPDQVR LLWTDPRNVG WRDKTSYRWR LLHRPQVGYI RVKLYEGPQL
910 920 930 940 950
VADSGVIIDT SMRGGRLGVF CFSQENIIWS NLQYRCNDTV PEDFEPFRRQ

LLQGRV
Length:956
Mass (Da):104,119
Last modified:July 27, 2011 - v2
Checksum:i4FAC268D11C83CAA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti433L → F in AAA40433 (PubMed:8288588).Curated1
Sequence conflicti563D → N in AAA40497 (PubMed:8468055).Curated1
Sequence conflicti563D → N in AAA40430 (PubMed:1601886).Curated1
Sequence conflicti720E → G in AAA40497 (PubMed:8468055).Curated1
Sequence conflicti720E → G in AAA40430 (PubMed:1601886).Curated1
Sequence conflicti871W → L in AAA40497 (PubMed:8468055).Curated1
Sequence conflicti871W → L in AAA40430 (PubMed:1601886).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04302 mRNA. Translation: AAA40497.1.
L24434 mRNA. Translation: AAA40433.1.
CH466547 Genomic DNA. Translation: EDL15225.1.
BC053023 mRNA. Translation: AAH53023.1.
M86620
, M86611, M86612, M86613, M86614, M86615, M86616, M86617, M86618, M86619 Genomic DNA. Translation: AAA40430.1.
U16175 Genomic DNA. Translation: AAA98537.1.
U66257 Genomic DNA. Translation: AAC52819.1.
CCDSiCCDS17495.1.
PIRiA46016.
RefSeqiNP_038719.2. NM_013691.2.
UniGeneiMm.2114.

Genome annotation databases

EnsembliENSMUST00000029682; ENSMUSP00000029682; ENSMUSG00000028047.
GeneIDi21827.
KEGGimmu:21827.
UCSCiuc008pyg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04302 mRNA. Translation: AAA40497.1.
L24434 mRNA. Translation: AAA40433.1.
CH466547 Genomic DNA. Translation: EDL15225.1.
BC053023 mRNA. Translation: AAH53023.1.
M86620
, M86611, M86612, M86613, M86614, M86615, M86616, M86617, M86618, M86619 Genomic DNA. Translation: AAA40430.1.
U16175 Genomic DNA. Translation: AAA98537.1.
U66257 Genomic DNA. Translation: AAC52819.1.
CCDSiCCDS17495.1.
PIRiA46016.
RefSeqiNP_038719.2. NM_013691.2.
UniGeneiMm.2114.

3D structure databases

ProteinModelPortaliQ05895.
SMRiQ05895.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029682.

PTM databases

PhosphoSitePlusiQ05895.

Proteomic databases

MaxQBiQ05895.
PaxDbiQ05895.
PRIDEiQ05895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029682; ENSMUSP00000029682; ENSMUSG00000028047.
GeneIDi21827.
KEGGimmu:21827.
UCSCiuc008pyg.2. mouse.

Organism-specific databases

CTDi7059.
MGIiMGI:98739. Thbs3.

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
GeneTreeiENSGT00850000132273.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiQ05895.
KOiK04659.
OMAiYQDSGRF.
OrthoDBiEOG091G00TV.
TreeFamiTF324917.

Enzyme and pathway databases

ReactomeiR-MMU-186797. Signaling by PDGF.

Miscellaneous databases

PROiQ05895.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028047.
CleanExiMM_THBS3.
ExpressionAtlasiQ05895. baseline and differential.
GenevisibleiQ05895. MM.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR028507. Thrombospondin-3.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PANTHERiPTHR10199:SF89. PTHR10199:SF89. 1 hit.
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSP3_MOUSE
AccessioniPrimary (citable) accession number: Q05895
Secondary accession number(s): Q6LCE0, Q7TN15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.