ID   PCKG_ASCSU              Reviewed;         643 AA.
AC   Q05893;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   16-JUN-2009, entry version 50.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase [GTP];
DE            Short=PEPCK;
DE            EC=4.1.1.32;
DE   AltName: Full=Phosphoenolpyruvate carboxylase;
GN   Name=PEPCK;
OS   Ascaris suum (Pig roundworm) (Ascaris lumbricoides).
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Ascaridida; Ascaridoidea;
OC   Ascarididae; Ascaris.
OX   NCBI_TaxID=6253;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Nerve cord, and Pharynx;
RX   MEDLINE=93387435; PubMed=8375484; DOI=10.1006/expr.1993.1072;
RA   Geary T.G., Winterrowd C.A., Alexander-Bowman S.J., Favreau M.A.,
RA   Nulf S.C., Klein R.D.;
RT   "Ascaris suum: cloning of a cDNA encoding phosphoenolpyruvate
RT   carboxykinase.";
RL   Exp. Parasitol. 77:155-161(1993).
CC   -!- FUNCTION: In parasitic nematodes PEPCK carboxylates
CC       phosphoenolpyruvate to oxaloacetate thus introducing the products
CC       of glycolysis to mitochondrial metabolism.
CC   -!- FUNCTION: Catalyzes the conversion of oxaloacetate (OAA) to
CC       phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic
CC       pathway that produces glucose from lactate and other precursors
CC       derived from the citric acid cycle (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + oxaloacetate = GDP + phosphoenolpyruvate
CC       + CO(2).
CC   -!- COFACTOR: Binds 1 manganese ion per subunit (By similarity).
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC       family.
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DR   EMBL; L01787; AAA29378.1; -; mRNA.
DR   HSSP; P35558; 1KHB.
DR   BRENDA; 4.1.1.32; 649.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) act...; IEA:EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:InterPro.
DR   InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008209; PEP_carboxykinase_GTP.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   Gene3D; G3DSA:3.90.228.20; PEP_carboxykinase_C; 1.
DR   Gene3D; G3DSA:3.40.449.10; PEP_carboxykinase_N; 1.
DR   PANTHER; PTHR11561; PEP_carboxykin; 1.
DR   Pfam; PF00821; PEPCK; 1.
DR   PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 1.
DR   ProDom; PD004738; PEPCK_N; 1.
DR   PROSITE; PS00505; PEPCK_GTP; 1.
PE   2: Evidence at transcript level;
KW   Decarboxylase; GTP-binding; Lyase; Manganese; Metal-binding;
KW   Nucleotide-binding.
FT   CHAIN         1    643       Phosphoenolpyruvate carboxykinase [GTP].
FT                                /FTId=PRO_0000103632.
FT   NP_BIND     305    310       GTP (By similarity).
FT   NP_BIND     548    551       GTP (By similarity).
FT   REGION      422    424       Substrate binding (By similarity).
FT   ACT_SITE    306    306       By similarity.
FT   METAL       262    262       Manganese (By similarity).
FT   METAL       282    282       Manganese (By similarity).
FT   METAL       329    329       Manganese (By similarity).
FT   BINDING     102    102       Substrate (By similarity).
FT   BINDING     255    255       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     262    262       Substrate (By similarity).
FT   BINDING     304    304       Substrate (By similarity).
FT   BINDING     424    424       GTP (By similarity).
FT   BINDING     455    455       GTP (By similarity).
SQ   SEQUENCE   643 AA;  72230 MW;  A9C369959AA79E27 CRC64;
     MRCRSLSHFK DDDFAVVSEV VTHKQNHIPV IKGDFVSLPK HVQRFVAEKA ELMKPSAIFI
     CDGSQNEADE LIARCVERGV LVPLKAYKNN YLCRTDPRDV ARVESKTWMI TPEKYDSVCH
     TPEGVKPMMG QWMSPDEFGK ELDDRFPGCM AGRTMYVIPY SMGPVGGPLS KIGIELTDSD
     YVVLCMRIMT RMGEPVLKAL AKNNGEFVRC VHSVGQPKPV ATKVINHWPC NPEKTIIAHR
     PAEREIWSFG SGYGGNSLLG KKCFALRIAM NIGYDEGWMA EHMLIMGVTS PKGEERFVAA
     AFPSACGKTN LAMLEPTIPG WKVRVIGDDI AWMKFGADGR LYAINPEYGF FGVAPGTSHK
     TNPMAMASFQ ENTIFTNVAE TADGEYFWEG LEHEVKNPKV DMINWLGEPW HIGDESKAAH
     PNSRFTAPAG QCPIIHPDWE KPEGVPIDAI IFGGRRPEGV PLVFESRSWV HGIFVGACVK
     SEATAAAEHT GKQVMHDPMA MRPFMGYNFG RYMRHWMKLG QPPHKVPKIF HVNWFRQSAD
     HKFLWPGYGD NIRVIDWILR RCSGDATIAE ETPIGFIPKK GTINLEGLPN VNWDELMSIP
     KSYWLEDMVE TKTFFENQVG SDLPPEIAKE LEAQTERIKA LKE
//