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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

PEPCK

Organism
Ascaris suum (Pig roundworm) (Ascaris lumbricoides)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

In parasitic nematodes PEPCK carboxylates phosphoenolpyruvate to oxaloacetate thus introducing the products of glycolysis to mitochondrial metabolism.
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102SubstrateBy similarity1
Metal bindingi262ManganeseBy similarity1
Metal bindingi282Manganese; via tele nitrogenBy similarity1
Binding sitei304SubstrateBy similarity1
Active sitei306By similarity1
Metal bindingi329ManganeseBy similarity1
Binding sitei424GTPBy similarity1
Binding sitei455GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi305 – 310GTPBy similarity6
Nucleotide bindingi548 – 551GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18260.
SABIO-RKQ05893.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
Gene namesi
Name:PEPCK
OrganismiAscaris suum (Pig roundworm) (Ascaris lumbricoides)
Taxonomic identifieri6253 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaAscarididaAscaridoideaAscarididaeAscaris

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036321 – 643Phosphoenolpyruvate carboxykinase [GTP]Add BLAST643

Proteomic databases

PRIDEiQ05893.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ05893.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 255Substrate bindingBy similarity3
Regioni422 – 424Substrate bindingBy similarity3

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCRSLSHFK DDDFAVVSEV VTHKQNHIPV IKGDFVSLPK HVQRFVAEKA
60 70 80 90 100
ELMKPSAIFI CDGSQNEADE LIARCVERGV LVPLKAYKNN YLCRTDPRDV
110 120 130 140 150
ARVESKTWMI TPEKYDSVCH TPEGVKPMMG QWMSPDEFGK ELDDRFPGCM
160 170 180 190 200
AGRTMYVIPY SMGPVGGPLS KIGIELTDSD YVVLCMRIMT RMGEPVLKAL
210 220 230 240 250
AKNNGEFVRC VHSVGQPKPV ATKVINHWPC NPEKTIIAHR PAEREIWSFG
260 270 280 290 300
SGYGGNSLLG KKCFALRIAM NIGYDEGWMA EHMLIMGVTS PKGEERFVAA
310 320 330 340 350
AFPSACGKTN LAMLEPTIPG WKVRVIGDDI AWMKFGADGR LYAINPEYGF
360 370 380 390 400
FGVAPGTSHK TNPMAMASFQ ENTIFTNVAE TADGEYFWEG LEHEVKNPKV
410 420 430 440 450
DMINWLGEPW HIGDESKAAH PNSRFTAPAG QCPIIHPDWE KPEGVPIDAI
460 470 480 490 500
IFGGRRPEGV PLVFESRSWV HGIFVGACVK SEATAAAEHT GKQVMHDPMA
510 520 530 540 550
MRPFMGYNFG RYMRHWMKLG QPPHKVPKIF HVNWFRQSAD HKFLWPGYGD
560 570 580 590 600
NIRVIDWILR RCSGDATIAE ETPIGFIPKK GTINLEGLPN VNWDELMSIP
610 620 630 640
KSYWLEDMVE TKTFFENQVG SDLPPEIAKE LEAQTERIKA LKE
Length:643
Mass (Da):72,230
Last modified:February 1, 1994 - v1
Checksum:iA9C369959AA79E27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01787 mRNA. Translation: AAA29378.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01787 mRNA. Translation: AAA29378.1.

3D structure databases

ProteinModelPortaliQ05893.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ05893.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18260.
SABIO-RKQ05893.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_ASCSU
AccessioniPrimary (citable) accession number: Q05893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 5, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.