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Q05893

- PCKG_ASCSU

UniProt

Q05893 - PCKG_ASCSU

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
PEPCK
Organism
Ascaris suum (Pig roundworm) (Ascaris lumbricoides)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

In parasitic nematodes PEPCK carboxylates phosphoenolpyruvate to oxaloacetate thus introducing the products of glycolysis to mitochondrial metabolism.UniRule annotation
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021Substrate By similarity
Binding sitei255 – 2551Substrate; via amide nitrogen By similarity
Metal bindingi262 – 2621Manganese By similarity
Binding sitei262 – 2621Substrate By similarity
Metal bindingi282 – 2821Manganese; via tele nitrogen By similarity
Binding sitei304 – 3041Substrate By similarity
Active sitei306 – 3061 By similarity
Metal bindingi329 – 3291Manganese By similarity
Binding sitei424 – 4241GTP By similarity
Binding sitei455 – 4551GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi305 – 3106GTP By similarity
Nucleotide bindingi548 – 5514GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18260.
SABIO-RKQ05893.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
Gene namesi
Name:PEPCK
OrganismiAscaris suum (Pig roundworm) (Ascaris lumbricoides)
Taxonomic identifieri6253 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaAscarididaAscaridoideaAscarididaeAscaris

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 643643Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_0000103632Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ05893.
SMRiQ05893. Positions 27-641.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni422 – 4243Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05893-1 [UniParc]FASTAAdd to Basket

« Hide

MRCRSLSHFK DDDFAVVSEV VTHKQNHIPV IKGDFVSLPK HVQRFVAEKA    50
ELMKPSAIFI CDGSQNEADE LIARCVERGV LVPLKAYKNN YLCRTDPRDV 100
ARVESKTWMI TPEKYDSVCH TPEGVKPMMG QWMSPDEFGK ELDDRFPGCM 150
AGRTMYVIPY SMGPVGGPLS KIGIELTDSD YVVLCMRIMT RMGEPVLKAL 200
AKNNGEFVRC VHSVGQPKPV ATKVINHWPC NPEKTIIAHR PAEREIWSFG 250
SGYGGNSLLG KKCFALRIAM NIGYDEGWMA EHMLIMGVTS PKGEERFVAA 300
AFPSACGKTN LAMLEPTIPG WKVRVIGDDI AWMKFGADGR LYAINPEYGF 350
FGVAPGTSHK TNPMAMASFQ ENTIFTNVAE TADGEYFWEG LEHEVKNPKV 400
DMINWLGEPW HIGDESKAAH PNSRFTAPAG QCPIIHPDWE KPEGVPIDAI 450
IFGGRRPEGV PLVFESRSWV HGIFVGACVK SEATAAAEHT GKQVMHDPMA 500
MRPFMGYNFG RYMRHWMKLG QPPHKVPKIF HVNWFRQSAD HKFLWPGYGD 550
NIRVIDWILR RCSGDATIAE ETPIGFIPKK GTINLEGLPN VNWDELMSIP 600
KSYWLEDMVE TKTFFENQVG SDLPPEIAKE LEAQTERIKA LKE 643
Length:643
Mass (Da):72,230
Last modified:February 1, 1994 - v1
Checksum:iA9C369959AA79E27
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01787 mRNA. Translation: AAA29378.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01787 mRNA. Translation: AAA29378.1 .

3D structure databases

ProteinModelPortali Q05893.
SMRi Q05893. Positions 27-641.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-18260.
SABIO-RK Q05893.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Ascaris suum: cloning of a cDNA encoding phosphoenolpyruvate carboxykinase."
    Geary T.G., Winterrowd C.A., Alexander-Bowman S.J., Favreau M.A., Nulf S.C., Klein R.D.
    Exp. Parasitol. 77:155-161(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Nerve cord and Pharynx.

Entry informationi

Entry nameiPCKG_ASCSU
AccessioniPrimary (citable) accession number: Q05893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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