Reviewed, UniProtKB/Swiss-Prot Q05888 (UDPK_STRMU)

Names and origin

Protein names

Recommended name:
    Undecaprenol kinase
      Short name=UdpK
    EC=2.7.1.66

Gene names

Name:	dgkA
Synonyms:	dgk
Ordered Locus Names:	SMU_1618

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of undecaprenol, which is probably the primary physiological substrate. Is also able to phosphorylate diacylglycerol, albeit with very low efficiency. May play a role in adaptability to environmental stress conditions such as acid tolerance, elevated temperatures and high osmolarity. Ref.4
Catalytic activity	ATP + undecaprenol = ADP + undecaprenyl phosphate. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein. Ref.4
Disruption phenotype	Cells lacking this gene display defective growth at low pH, elevated temperatures, and high osmolarity. Moreover, they exhibit a significant reduction in the transcription level of mutA and a defect in production of the lantibiotic mutacin II. They also show reduced resistance to bacitracin. Ref.4 Ref.1 Ref.2
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.
Caution	It is uncertain whether Met-1 or Met-3 is the initiator.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis Stress response
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	Undecaprenol kinase		PRO_0000195269
Regions
	Topological domain	1 – 42	42	Cytoplasmic Potential
	Transmembrane	43 – 58	16	Potential
	Transmembrane	62 – 78	17	Potential
	Topological domain	79 – 108	30	Cytoplasmic Potential
	Transmembrane	109 – 129	21	Potential
Experimental info
	Sequence conflict	128 – 129	2	VP → LL in AAA26867. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05888-1 [UniParc]. Last modified November 28, 2002. Version 2. Checksum: 5471974885319125	FASTA	137	15,350
	10 20 30 40 50 60 MPMDLRDNKQ SQKKWKNRTL TSSLEFALTG IFTAFKEERN MKKHAVSALL AVIAGLVFKV 70 80 90 100 110 120 SVIEWLFLLL SIFLVITFEI VNSAIENVVD LASDYHFSML AKNAKDMAAG AVLVISGFAA 130 LTGLIIFVPK IWFLLFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a Streptococcus mutans mutant altered in environmental stress responses." Yamashita Y., Takehara T., Kuramitsu H.K. J. Bacteriol. 175:6220-6228(1993) [PubMed: 8407794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: GS-5.
[2]	"Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans." Chen P., Novak J., Qi F.-Q., Caufield P.W. J. Bacteriol. 180:167-170(1998) [PubMed: 9422608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: T8.
[3]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.
[4]	"The stress-responsive dgk gene from Streptococcus mutans encodes a putative undecaprenol kinase activity." Lis M., Kuramitsu H.K. Infect. Immun. 71:1938-1943(2003) [PubMed: 12654811] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE. Strain: GS-5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12211 Genomic DNA. Translation: AAA26867.1. AF000954 Genomic DNA. Translation: AAC38047.1. AE014133 Genomic DNA. Translation: AAN59259.1.
PIR	A36933.
RefSeq	NP_721953.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1028846.
GenomeReviews	Gene locus SMU_1618 in contig AE014133_GR.
KEGG	smu:SMU.1618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q05888.
OMA	LASINSV.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_1618-MON.
BRENDA	2.7.1.107. 20716.
Family and domain databases
InterPro	IPR000829. Diacylglycerol_kinase_prok. [Graphical view]
Pfam	PF01219. DAGK_prokar. 1 hit. [Graphical view]
ProDom	PD010722. DAGK_prokar. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01069. DAGK_PROKAR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UDPK_STRMU

Accession

Primary (citable) accession number: Q05888
Secondary accession number(s): O51807

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 28, 2002
Last modified:	November 3, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)