ID   NNT1_YEAST              Reviewed;         261 AA.
AC   Q05874;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=Putative nicotinamide N-methyltransferase;
DE            EC=2.1.1.1;
GN   Name=NNT1; OrderedLocusNames=YLR285W;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313267; PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
RA   Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
RA   Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
RA   Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
RA   Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
RA   Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
RA   Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
RA   Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
RA   Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
RA   Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
RA   Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
RA   Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [3]
RP   FUNCTION.
RX   PubMed=12736687; DOI=10.1038/nature01578;
RA   Anderson R.M., Bitterman K.J., Wood J.G., Medvedik O., Sinclair D.A.;
RT   "Nicotinamide and PNC1 govern lifespan extension by calorie
RT   restriction in Saccharomyces cerevisiae.";
RL   Nature 423:181-185(2003).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Putative nicotinamide N-methyltransferase involved in
CC       rDNA silencing and in lifespan determination.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + nicotinamide = S-
CC       adenosyl-L-homocysteine + 1-methylnicotinamide.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Present with 7280 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the NNT1 family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; U17243; AAB67330.1; -; Genomic_DNA.
DR   EMBL; AY693038; AAT93057.1; -; Genomic_DNA.
DR   PIR; S50370; S50370.
DR   RefSeq; NP_013387.1; -.
DR   DIP; DIP:2117N; -.
DR   IntAct; Q05874; 3.
DR   PeptideAtlas; Q05874; -.
DR   Ensembl; YLR285W; Saccharomyces cerevisiae.
DR   GeneID; 850991; -.
DR   GenomeReviews; Y13138_GR; YLR285W.
DR   KEGG; sce:YLR285W; -.
DR   NMPDR; fig|4932.3.peg.4402; -.
DR   CYGD; YLR285w; -.
DR   SGD; S000004275; NNT1.
DR   HOGENOM; Q05874; -.
DR   OMA; Q05874; VVFSPHR.
DR   BRENDA; 2.1.1.1; 250.
DR   NextBio; 967516; -.
DR   GermOnline; YLR285W; Saccharomyces cerevisiae.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0008112; F:nicotinamide N-methyltransferase activity; IEA:EC.
DR   GO; GO:0000183; P:chromatin silencing at rDNA; IMP:SGD.
DR   GO; GO:0006769; P:nicotinamide metabolic process; IGI:SGD.
DR   InterPro; IPR019410; Methyltransferase-16_put.
DR   Pfam; PF10294; Methyltransf_16; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Methyltransferase;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    261       Putative nicotinamide N-
FT                                methyltransferase.
FT                                /FTId=PRO_0000096901.
SQ   SEQUENCE   261 AA;  29633 MW;  74AC77E39ED55387 CRC64;
     MSDIESLGEA AGLFEEPEDF LPPPPKPHFA EYQRSHITKE SKSDVKDIKL RLVGTSPLWG
     HLLWNAGIYT ANHLDSHPEL IKGKTVLELG AAAALPSVIC ALNGAQMVVS TDYPDPDLMQ
     NIDYNIKSNV PEDFNNVSTE GYIWGNDYSP LLAHIEKIGN NNGKFDLIIL SDLVFNHTEH
     HKLLQTTKDL LAEKGQALVV FSPHRPKLLE KDLEFFELAK NEFHLVPQLI EMVNWKPMFD
     EDEETIEVRS RVYAYYLTHE K
//