Reviewed, UniProtKB/Swiss-Prot Q05874 (NNT1_YEAST)

Names and origin

Protein names

Recommended name:
    Putative nicotinamide N-methyltransferase
    EC=2.1.1.1

Gene names

Name:	NNT1
Ordered Locus Names:	YLR285W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative nicotinamide N-methyltransferase involved in rDNA silencing and in lifespan determination. Ref.3
Catalytic activity	S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.
Subcellular location	Cytoplasm Ref.4.
Miscellaneous	Present with 7280 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the NNT1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin silencing at rDNA Ref.3 Inferred from mutant phenotype. Source: SGD nicotinamide metabolic process Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD
Molecular function	nicotinamide N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Putative nicotinamide N-methyltransferase		PRO_0000096901

Sequences

	Sequence		Length	Mass (Da)
	Q05874-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74AC77E39ED55387	FASTA	261	29,633
	10 20 30 40 50 60 MSDIESLGEA AGLFEEPEDF LPPPPKPHFA EYQRSHITKE SKSDVKDIKL RLVGTSPLWG 70 80 90 100 110 120 HLLWNAGIYT ANHLDSHPEL IKGKTVLELG AAAALPSVIC ALNGAQMVVS TDYPDPDLMQ 130 140 150 160 170 180 NIDYNIKSNV PEDFNNVSTE GYIWGNDYSP LLAHIEKIGN NNGKFDLIIL SDLVFNHTEH 190 200 210 220 230 240 HKLLQTTKDL LAEKGQALVV FSPHRPKLLE KDLEFFELAK NEFHLVPQLI EMVNWKPMFD 250 260 EDEETIEVRS RVYAYYLTHE K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae." Anderson R.M., Bitterman K.J., Wood J.G., Medvedik O., Sinclair D.A. Nature 423:181-185(2003) [PubMed: 12736687] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17243 Genomic DNA. Translation: AAB67330.1. AY693038 Genomic DNA. Translation: AAT93057.1.
PIR	S50370.
RefSeq	NP_013387.1.
3D structure databases
SMR	Q05874. Positions 59-225.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2117N.
IntAct	Q05874. 3 interactions.
STRING	Q05874.
Proteomic databases
PeptideAtlas	Q05874.
Genome annotation databases
Ensembl	YLR285W; YLR285W; YLR285W; Saccharomyces cerevisiae. [Genome view]
GeneID	850991.
KEGG	sce:YLR285W.
NMPDR	fig|4932.3.peg.4402.
Organism-specific databases
CYGD	YLR285w.
SGD	S000004275. NNT1.
Phylogenomic databases
eggNOG	fuNOG05731.
HOGENOM	HBG397955.
OMA	VVFSPHR.
OrthoDB	EOG92BZS7.
PhylomeDB	Q05874.
Enzyme and pathway databases
BRENDA	2.1.1.1. 250.
Gene expression databases
ArrayExpress	Q05874.
Genevestigator	Q05874.
GermOnline	YLR285W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019410. Methyltransferase-16_put. [Graphical view]
Pfam	PF10294. Methyltransf_16. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967516.

Entry information

Entry name

NNT1_YEAST

Accession

Primary (citable) accession number: Q05874

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)