Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein N-methyltransferase NNT1

Gene

NNT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent protein methyltransferase. Involved in rDNA silencing and in lifespan determination.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei112 – 1121S-adenosyl-L-methionineBy similarity
Binding sitei144 – 1441S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei171 – 1711S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

  • N-terminal protein N-methyltransferase activity Source: SGD
  • protein-lysine N-methyltransferase activity Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD

GO - Biological processi

  • chromatin silencing at rDNA Source: SGD
  • N-terminal peptidyl-glycine methylation Source: SGD
  • peptidyl-lysine dimethylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-32380-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-methyltransferase NNT1Curated (EC:2.1.1.-1 Publication)
Alternative name(s):
Nicotinamide N-methyltransferase-like protein 11 Publication
Gene namesi
Name:NNT11 Publication
Ordered Locus Names:YLR285WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR285W.
SGDiS000004275. NNT1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Protein N-methyltransferase NNT1PRO_0000096901Add
BLAST

Proteomic databases

MaxQBiQ05874.
PeptideAtlasiQ05874.

Interactioni

Protein-protein interaction databases

BioGridi31550. 26 interactions.
DIPiDIP-2117N.
MINTiMINT-493718.

Structurei

3D structure databases

ProteinModelPortaliQ05874.
SMRiQ05874. Positions 71-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni90 – 923S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. NNT1 family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000199189.
InParanoidiQ05874.
KOiK17878.
OMAiLVFYTHH.
OrthoDBiEOG7JHMGG.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019410. Methyltransf_16.
IPR025784. Nicotinamide_N-MeTfrase_put.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51560. SAM_MT_NNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIESLGEA AGLFEEPEDF LPPPPKPHFA EYQRSHITKE SKSDVKDIKL
60 70 80 90 100
RLVGTSPLWG HLLWNAGIYT ANHLDSHPEL IKGKTVLELG AAAALPSVIC
110 120 130 140 150
ALNGAQMVVS TDYPDPDLMQ NIDYNIKSNV PEDFNNVSTE GYIWGNDYSP
160 170 180 190 200
LLAHIEKIGN NNGKFDLIIL SDLVFNHTEH HKLLQTTKDL LAEKGQALVV
210 220 230 240 250
FSPHRPKLLE KDLEFFELAK NEFHLVPQLI EMVNWKPMFD EDEETIEVRS
260
RVYAYYLTHE K
Length:261
Mass (Da):29,633
Last modified:November 1, 1996 - v1
Checksum:i74AC77E39ED55387
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17243 Genomic DNA. Translation: AAB67330.1.
AY693038 Genomic DNA. Translation: AAT93057.1.
BK006945 Genomic DNA. Translation: DAA09595.1.
PIRiS50370.
RefSeqiNP_013387.1. NM_001182172.1.

Genome annotation databases

EnsemblFungiiYLR285W; YLR285W; YLR285W.
GeneIDi850991.
KEGGisce:YLR285W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17243 Genomic DNA. Translation: AAB67330.1.
AY693038 Genomic DNA. Translation: AAT93057.1.
BK006945 Genomic DNA. Translation: DAA09595.1.
PIRiS50370.
RefSeqiNP_013387.1. NM_001182172.1.

3D structure databases

ProteinModelPortaliQ05874.
SMRiQ05874. Positions 71-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31550. 26 interactions.
DIPiDIP-2117N.
MINTiMINT-493718.

Proteomic databases

MaxQBiQ05874.
PeptideAtlasiQ05874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR285W; YLR285W; YLR285W.
GeneIDi850991.
KEGGisce:YLR285W.

Organism-specific databases

EuPathDBiFungiDB:YLR285W.
SGDiS000004275. NNT1.

Phylogenomic databases

HOGENOMiHOG000199189.
InParanoidiQ05874.
KOiK17878.
OMAiLVFYTHH.
OrthoDBiEOG7JHMGG.

Enzyme and pathway databases

BioCyciYEAST:G3O-32380-MONOMER.

Miscellaneous databases

NextBioi967516.
PROiQ05874.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019410. Methyltransf_16.
IPR025784. Nicotinamide_N-MeTfrase_put.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51560. SAM_MT_NNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae."
    Anderson R.M., Bitterman K.J., Wood J.G., Medvedik O., Sinclair D.A.
    Nature 423:181-185(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Comprehensive structural and substrate specificity classification of the Saccharomyces cerevisiae methyltransferome."
    Wlodarski T., Kutner J., Towpik J., Knizewski L., Rychlewski L., Kudlicki A., Rowicka M., Dziembowski A., Ginalski K.
    PLoS ONE 6:E23168-E23168(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION.

Entry informationi

Entry nameiNNT1_YEAST
AccessioniPrimary (citable) accession number: Q05874
Secondary accession number(s): D6VYS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.