Q05874 (NNT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative nicotinamide N-methyltransferase
EC=2.1.1.1

Gene names

Name:	NNT1
Ordered Locus Names:	YLR285W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative nicotinamide N-methyltransferase involved in rDNA silencing and in lifespan determination. Ref.4
Catalytic activity	S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 7280 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the NNT1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin silencing at rDNA Inferred from mutant phenotype Ref.4. Source: SGD nicotinamide metabolic process Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.5. Source: SGD
Molecular function	nicotinamide N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Putative nicotinamide N-methyltransferase		PRO_0000096901

Sequences

	Sequence		Length	Mass (Da)
	Q05874 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74AC77E39ED55387	FASTA	261	29,633
	10 20 30 40 50 60 MSDIESLGEA AGLFEEPEDF LPPPPKPHFA EYQRSHITKE SKSDVKDIKL RLVGTSPLWG 70 80 90 100 110 120 HLLWNAGIYT ANHLDSHPEL IKGKTVLELG AAAALPSVIC ALNGAQMVVS TDYPDPDLMQ 130 140 150 160 170 180 NIDYNIKSNV PEDFNNVSTE GYIWGNDYSP LLAHIEKIGN NNGKFDLIIL SDLVFNHTEH 190 200 210 220 230 240 HKLLQTTKDL LAEKGQALVV FSPHRPKLLE KDLEFFELAK NEFHLVPQLI EMVNWKPMFD 250 260 EDEETIEVRS RVYAYYLTHE K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae." Anderson R.M., Bitterman K.J., Wood J.G., Medvedik O., Sinclair D.A. Nature 423:181-185(2003) [PubMed: 12736687] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17243 Genomic DNA. Translation: AAB67330.1. AY693038 Genomic DNA. Translation: AAT93057.1. BK006945 Genomic DNA. Translation: DAA09595.1.
PIR	S50370.
RefSeq	NP_013387.1. NM_001182172.1.
3D structure databases
ProteinModelPortal	Q05874.
SMR	Q05874. Positions 38-260.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2117N.
MINT	MINT-493718.
STRING	Q05874.
Proteomic databases
PeptideAtlas	Q05874.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR285W; YLR285W; YLR285W.
GeneID	850991.
KEGG	sce:YLR285W.
NMPDR	fig|4932.3.peg.4402.
Organism-specific databases
CYGD	YLR285w.
SGD	S000004275. NNT1.
Phylogenomic databases
eggNOG	fuNOG05731.
GeneTree	EFGT00050000001751.
HOGENOM	HBG397955.
OMA	HLLWNAG.
OrthoDB	EOG4MKRRQ.
Gene expression databases
ArrayExpress	Q05874.
Genevestigator	Q05874.
GermOnline	YLR285W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019410. Nicotinamide_N-MeTfrase_put. [Graphical view]
Pfam	PF10294. Methyltransf_16. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967516.

Entry information

Entry name

NNT1_YEAST

Accession

Primary (citable) accession number: Q05874
Secondary accession number(s): D6VYS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program