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Protein

Valine--tRNA ligase

Gene

valS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity). Catalyzes the attachment of valine to tRNA(Val).By similarity

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei528ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase, Repressor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU28090-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
Alternative name(s):
Valyl-tRNA synthetaseUniRule annotation
Short name:
ValRSUniRule annotation
Gene namesi
Name:valSUniRule annotation
Ordered Locus Names:BSU28090
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene grow normally, suggesting there is a second functional gene with valine-tRNA synthetase activity in B.subtilis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062141 – 880Valine--tRNA ligaseAdd BLAST880

Proteomic databases

PaxDbiQ05873.

Expressioni

Inductioni

By valine starvation. Is transcribed by itself and in an operon with folC. A GUC triplet (Val, the specifier codon) 190 nucleotides upstream of the initiator codon confers induction upon valaine starvation; replacing it with ACC (Thr) confers induction upon threonine starvation, replacing it with UAA (stop), renders the gene uninducible. Negatively regulates its own transcription; this depends on the presence of the GUC specifier codon.1 Publication

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015351.

Structurei

3D structure databases

ProteinModelPortaliQ05873.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili809 – 879UniRule annotationAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 59"HIGH" regionAdd BLAST11
Motifi525 – 529"KMSKS" region5

Domaini

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
HOGENOMiHOG000020093.
InParanoidiQ05873.
KOiK01873.
OMAiSQYRFDL.
PhylomeDBiQ05873.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.287.380. 1 hit.
1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 2 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05873-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METNEQTMPT KYDPAAVEKD RYDFWLKGKF FEAGSDQTKE PYSVVIPPPN
60 70 80 90 100
VTGRLHLGHA WDTTLQDIVT RMKRMQGYDV LWLPGMDHAG IATQAKVEAK
110 120 130 140 150
LREEGKSRYD LGREKFLEET WKWKEEYADF IRSQWAKLGL GLDYSRERFT
160 170 180 190 200
LDEGLSKAVR EVFVKLYEKG LIYRGEYIIN WDPATKTALS DIEVIYKDVQ
210 220 230 240 250
GAFYHMSYPL ADGSGSIEIA TTRPETMLGD TAVAVHPEDE RYKHLIGKTV
260 270 280 290 300
ILPIVNREIP IVGDDYVDME FGSGAVKITP AHDPNDFELG NRHNLERILV
310 320 330 340 350
MNEDGTMNEN ALQYQGMDRF ECRKKLVKDL QEAGVLFKIE DHMHSVGHSE
360 370 380 390 400
RSGAVVEPYL STQWFVRMQP LADAAIELQK KEEKVNFVPD RFEKTYLHWM
410 420 430 440 450
ENIRDWCISR QLWWGHRIPA WYHKETGELY VGLEAPEDSE NWEQDTDVLD
460 470 480 490 500
TWFSSALWPF STMGWPDVTA EDFKRYYPTD VLVTGYDIIF FWVSRMIFQG
510 520 530 540 550
IEFTGERPFK DVLIHGLIRD EQGRKMSKSL GNGVDPMDVI DKYGADSLRY
560 570 580 590 600
FLATGSSPGQ DLRFSYEKVE STWNFANKIW NASRFALMNM DGMTYDELDL
610 620 630 640 650
SGEKSVADKW ILTRLNETIE HVTQLADRYE FGEVGRHLYN FIWDDFCDWY
660 670 680 690 700
IEMAKLPLYG EDEAAKKTTR SILAYVLDQT MRLLHPFMPF LTEEIWQHLP
710 720 730 740 750
HQGESITVSQ WPAVVPEHTD TEAAADMKLL VELIRSVRNI RSEVNTPMSK
760 770 780 790 800
QVELYIKTST DEIASRLEAN RSYVERFTNP SVLKIGTDIE AVDKAMTAVV
810 820 830 840 850
SGAEVILPLE GLINIDEEIA RLQKEFDKLT KEVERVQKKL GNEGFMKKAP
860 870 880
AHVIDEEREK EKDYVAKRDA VQKRMAELKG
Length:880
Mass (Da):101,745
Last modified:July 7, 2009 - v3
Checksum:iEAA0A4508F6283D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti379Q → E in CAA54458 (PubMed:9098041).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77239 Genomic DNA. Translation: CAA54458.1.
AL009126 Genomic DNA. Translation: CAB14769.2.
L04520 Genomic DNA. Translation: AAB59020.1.
PIRiS41420.
RefSeqiNP_390687.2. NC_000964.3.
WP_004398606.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14769; CAB14769; BSU28090.
GeneIDi937491.
KEGGibsu:BSU28090.
PATRICi18977492. VBIBacSub10457_2935.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77239 Genomic DNA. Translation: CAA54458.1.
AL009126 Genomic DNA. Translation: CAB14769.2.
L04520 Genomic DNA. Translation: AAB59020.1.
PIRiS41420.
RefSeqiNP_390687.2. NC_000964.3.
WP_004398606.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliQ05873.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015351.

Proteomic databases

PaxDbiQ05873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14769; CAB14769; BSU28090.
GeneIDi937491.
KEGGibsu:BSU28090.
PATRICi18977492. VBIBacSub10457_2935.

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
HOGENOMiHOG000020093.
InParanoidiQ05873.
KOiK01873.
OMAiSQYRFDL.
PhylomeDBiQ05873.

Enzyme and pathway databases

BioCyciBSUB:BSU28090-MONOMER.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.287.380. 1 hit.
1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 2 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYV_BACSU
AccessioniPrimary (citable) accession number: Q05873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 7, 2009
Last modified: October 5, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.