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Protein

3,2-trans-enoyl-CoA isomerase

Gene

ECI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the beta oxidation of unsaturated fatty acids.1 Publication

Catalytic activityi

(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126Substrate; via amide nitrogenCombined sources1 Publication1
Active sitei158Proton donor/acceptor1 Publication1

GO - Molecular functioni

  • dodecenoyl-CoA delta-isomerase activity Source: SGD

GO - Biological processi

  • fatty acid beta-oxidation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YLR284C-MONOMER.
YEAST:YLR284C-MONOMER.
BRENDAi5.3.3.8. 984.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
3,2-trans-enoyl-CoA isomerase (EC:5.3.3.8)
Alternative name(s):
Delta(3),Delta(2)-enoyl-CoA isomerase
Short name:
D3,D2-enoyl-CoA isomerase
Dodecenoyl-CoA isomerase
Gene namesi
Name:ECI1
Ordered Locus Names:YLR284C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR284C.
SGDiS000004274. ECI1.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi158E → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001092631 – 2803,2-trans-enoyl-CoA isomeraseAdd BLAST280

Proteomic databases

MaxQBiQ05871.
PRIDEiQ05871.

Expressioni

Inductioni

By oleate.1 Publication

Interactioni

Subunit structurei

Homohexamer, dimer of trimers. Interacts with DCI1.4 Publications

Protein-protein interaction databases

BioGridi31549. 11 interactors.
DIPiDIP-1692N.
IntActiQ05871. 4 interactors.
MINTiMINT-386405.

Structurei

Secondary structure

1280
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 16Combined sources6
Beta strandi19 – 24Combined sources6
Helixi27 – 29Combined sources3
Helixi35 – 50Combined sources16
Beta strandi56 – 60Combined sources5
Beta strandi65 – 67Combined sources3
Helixi73 – 75Combined sources3
Turni77 – 81Combined sources5
Beta strandi82 – 88Combined sources7
Helixi89 – 96Combined sources8
Helixi98 – 110Combined sources13
Beta strandi113 – 119Combined sources7
Helixi125 – 133Combined sources9
Beta strandi134 – 141Combined sources8
Beta strandi145 – 147Combined sources3
Helixi150 – 153Combined sources4
Helixi161 – 169Combined sources9
Helixi171 – 179Combined sources9
Helixi186 – 191Combined sources6
Beta strandi196 – 198Combined sources3
Helixi206 – 220Combined sources15
Turni221 – 223Combined sources3
Helixi226 – 237Combined sources12
Turni238 – 240Combined sources3
Helixi241 – 261Combined sources21
Turni263 – 266Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HNOX-ray2.50A1-280[»]
1HNUX-ray2.15A1-280[»]
1K39X-ray3.29A/B/C1-280[»]
1PJHX-ray2.10A/B/C1-280[»]
4ZDBX-ray2.14A/B/C1-280[»]
4ZDCX-ray2.13A/B/C1-280[»]
4ZDDX-ray3.00A1-280[»]
4ZDEX-ray2.10A/B/C1-280[»]
4ZDFX-ray1.81A/B1-268[»]
ProteinModelPortaliQ05871.
SMRiQ05871.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05871.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 72Substrate bindingCombined sources1 Publication5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi278 – 280Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027948.
InParanoidiQ05871.
KOiK13239.
OMAiLGTHLNQ.
OrthoDBiEOG092C431D.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEIRQNEK ISYRIEGPFF IIHLMNPDNL NALEGEDYIY LGELLELADR
60 70 80 90 100
NRDVYFTIIQ SSGRFFSSGA DFKGIAKAQG DDTNKYPSET SKWVSNFVAR
110 120 130 140 150
NVYVTDAFIK HSKVLICCLN GPAIGLSAAL VALCDIVYSI NDKVYLLYPF
160 170 180 190 200
ANLGLITEGG TTVSLPLKFG TNTTYECLMF NKPFKYDIMC ENGFISKNFN
210 220 230 240 250
MPSSNAEAFN AKVLEELREK VKGLYLPSCL GMKKLLKSNH IDAFNKANSV
260 270 280
EVNESLKYWV DGEPLKRFRQ LGSKQRKHRL
Length:280
Mass (Da):31,698
Last modified:November 1, 1996 - v1
Checksum:iCDB72D157ABB845C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090442 Genomic DNA. Translation: AAC83700.1.
U17243 Genomic DNA. Translation: AAB67329.1.
BK006945 Genomic DNA. Translation: DAA09594.1.
PIRiS50369.
RefSeqiNP_013386.1. NM_001182171.1.

Genome annotation databases

EnsemblFungiiYLR284C; YLR284C; YLR284C.
GeneIDi850990.
KEGGisce:YLR284C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090442 Genomic DNA. Translation: AAC83700.1.
U17243 Genomic DNA. Translation: AAB67329.1.
BK006945 Genomic DNA. Translation: DAA09594.1.
PIRiS50369.
RefSeqiNP_013386.1. NM_001182171.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HNOX-ray2.50A1-280[»]
1HNUX-ray2.15A1-280[»]
1K39X-ray3.29A/B/C1-280[»]
1PJHX-ray2.10A/B/C1-280[»]
4ZDBX-ray2.14A/B/C1-280[»]
4ZDCX-ray2.13A/B/C1-280[»]
4ZDDX-ray3.00A1-280[»]
4ZDEX-ray2.10A/B/C1-280[»]
4ZDFX-ray1.81A/B1-268[»]
ProteinModelPortaliQ05871.
SMRiQ05871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31549. 11 interactors.
DIPiDIP-1692N.
IntActiQ05871. 4 interactors.
MINTiMINT-386405.

Proteomic databases

MaxQBiQ05871.
PRIDEiQ05871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR284C; YLR284C; YLR284C.
GeneIDi850990.
KEGGisce:YLR284C.

Organism-specific databases

EuPathDBiFungiDB:YLR284C.
SGDiS000004274. ECI1.

Phylogenomic databases

GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027948.
InParanoidiQ05871.
KOiK13239.
OMAiLGTHLNQ.
OrthoDBiEOG092C431D.

Enzyme and pathway databases

UniPathwayiUPA00659.
BioCyciMetaCyc:YLR284C-MONOMER.
YEAST:YLR284C-MONOMER.
BRENDAi5.3.3.8. 984.

Miscellaneous databases

EvolutionaryTraceiQ05871.
PROiQ05871.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiECI1_YEAST
AccessioniPrimary (citable) accession number: Q05871
Secondary accession number(s): D6VYS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.