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Q05871

- ECI1_YEAST

UniProt

Q05871 - ECI1_YEAST

Protein

3,2-trans-enoyl-CoA isomerase

Gene

ECI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Essential for the beta oxidation of unsaturated fatty acids.1 Publication

    Catalytic activityi

    (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei126 – 1261Substrate; via amide nitrogen
    Sitei158 – 1581Important for catalytic activity

    GO - Molecular functioni

    1. dodecenoyl-CoA delta-isomerase activity Source: SGD

    GO - Biological processi

    1. fatty acid beta-oxidation Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR284C-MONOMER.
    YEAST:YLR284C-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3,2-trans-enoyl-CoA isomerase (EC:5.3.3.8)
    Alternative name(s):
    Delta(3),Delta(2)-enoyl-CoA isomerase
    Short name:
    D3,D2-enoyl-CoA isomerase
    Dodecenoyl-CoA isomerase
    Gene namesi
    Name:ECI1
    Ordered Locus Names:YLR284C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR284c.
    SGDiS000004274. ECI1.

    Subcellular locationi

    Peroxisome 3 Publications
    Note: This location is DCI1 dependent.

    GO - Cellular componenti

    1. peroxisome Source: SGD

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi158 – 1581E → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2802803,2-trans-enoyl-CoA isomerasePRO_0000109263Add
    BLAST

    Proteomic databases

    MaxQBiQ05871.
    PaxDbiQ05871.
    PeptideAtlasiQ05871.

    Expressioni

    Inductioni

    By oleate.1 Publication

    Gene expression databases

    GenevestigatoriQ05871.

    Interactioni

    Subunit structurei

    Homohexamer, dimer of trimers. Interacts with DCI1.4 Publications

    Protein-protein interaction databases

    BioGridi31549. 11 interactions.
    DIPiDIP-1692N.
    IntActiQ05871. 4 interactions.
    MINTiMINT-386405.
    STRINGi4932.YLR284C.

    Structurei

    Secondary structure

    1
    280
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi11 – 166
    Beta strandi19 – 246
    Helixi27 – 293
    Helixi35 – 5016
    Beta strandi56 – 605
    Beta strandi65 – 673
    Helixi72 – 765
    Turni77 – 815
    Helixi89 – 968
    Helixi98 – 11013
    Beta strandi113 – 1197
    Helixi125 – 1339
    Beta strandi134 – 1418
    Beta strandi145 – 1473
    Helixi150 – 1534
    Helixi161 – 1699
    Helixi171 – 1799
    Helixi186 – 1916
    Beta strandi196 – 1983
    Helixi206 – 22116
    Beta strandi222 – 2243
    Helixi226 – 23712
    Turni238 – 2403
    Helixi241 – 26121
    Helixi263 – 2686

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HNOX-ray2.50A1-280[»]
    1HNUX-ray2.15A1-280[»]
    1K39X-ray3.29A/B/C1-280[»]
    1PJHX-ray2.10A/B/C1-280[»]
    ProteinModelPortaliQ05871.
    SMRiQ05871. Positions 4-274.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ05871.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni68 – 725Substrate binding

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi278 – 2803Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1024.
    GeneTreeiENSGT00670000097595.
    HOGENOMiHOG000027948.
    OMAiLGTHLNQ.
    OrthoDBiEOG70PC7K.

    Family and domain databases

    Gene3Di3.90.226.10. 1 hit.
    InterProiIPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    [Graphical view]
    PfamiPF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF52096. SSF52096. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q05871-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQEIRQNEK ISYRIEGPFF IIHLMNPDNL NALEGEDYIY LGELLELADR    50
    NRDVYFTIIQ SSGRFFSSGA DFKGIAKAQG DDTNKYPSET SKWVSNFVAR 100
    NVYVTDAFIK HSKVLICCLN GPAIGLSAAL VALCDIVYSI NDKVYLLYPF 150
    ANLGLITEGG TTVSLPLKFG TNTTYECLMF NKPFKYDIMC ENGFISKNFN 200
    MPSSNAEAFN AKVLEELREK VKGLYLPSCL GMKKLLKSNH IDAFNKANSV 250
    EVNESLKYWV DGEPLKRFRQ LGSKQRKHRL 280
    Length:280
    Mass (Da):31,698
    Last modified:November 1, 1996 - v1
    Checksum:iCDB72D157ABB845C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090442 Genomic DNA. Translation: AAC83700.1.
    U17243 Genomic DNA. Translation: AAB67329.1.
    BK006945 Genomic DNA. Translation: DAA09594.1.
    PIRiS50369.
    RefSeqiNP_013386.1. NM_001182171.1.

    Genome annotation databases

    EnsemblFungiiYLR284C; YLR284C; YLR284C.
    GeneIDi850990.
    KEGGisce:YLR284C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090442 Genomic DNA. Translation: AAC83700.1 .
    U17243 Genomic DNA. Translation: AAB67329.1 .
    BK006945 Genomic DNA. Translation: DAA09594.1 .
    PIRi S50369.
    RefSeqi NP_013386.1. NM_001182171.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HNO X-ray 2.50 A 1-280 [» ]
    1HNU X-ray 2.15 A 1-280 [» ]
    1K39 X-ray 3.29 A/B/C 1-280 [» ]
    1PJH X-ray 2.10 A/B/C 1-280 [» ]
    ProteinModelPortali Q05871.
    SMRi Q05871. Positions 4-274.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31549. 11 interactions.
    DIPi DIP-1692N.
    IntActi Q05871. 4 interactions.
    MINTi MINT-386405.
    STRINGi 4932.YLR284C.

    Proteomic databases

    MaxQBi Q05871.
    PaxDbi Q05871.
    PeptideAtlasi Q05871.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR284C ; YLR284C ; YLR284C .
    GeneIDi 850990.
    KEGGi sce:YLR284C.

    Organism-specific databases

    CYGDi YLR284c.
    SGDi S000004274. ECI1.

    Phylogenomic databases

    eggNOGi COG1024.
    GeneTreei ENSGT00670000097595.
    HOGENOMi HOG000027948.
    OMAi LGTHLNQ.
    OrthoDBi EOG70PC7K.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci MetaCyc:YLR284C-MONOMER.
    YEAST:YLR284C-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q05871.
    NextBioi 967513.

    Gene expression databases

    Genevestigatori Q05871.

    Family and domain databases

    Gene3Di 3.90.226.10. 1 hit.
    InterProi IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    [Graphical view ]
    Pfami PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52096. SSF52096. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of Saccharomyces cerevisiae Delta3, Delta2-enoyl-CoA isomerase."
      Geisbrecht B.V., Zhu D., Schulz K., Nau K., Morrell J.C., Geraghty M.T., Schulz H., Erdmann R., Gould S.J.
      J. Biol. Chem. 273:33184-33191(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Preliminary characterization of Yor180Cp: identification of a novel peroxisomal protein of Saccharomyces cerevisiae involved in fatty acid metabolism."
      Geisbrecht B.V., Schulz K., Nau K., Geraghty M.T., Schulz H., Erdmann R., Gould S.J.
      Biochem. Biophys. Res. Commun. 260:28-34(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DCI1.
    5. "Crystallization and X-ray diffraction analysis of peroxisomal Delta3-Delta2-enoyl-CoA isomerase from Saccharomyces cerevisiae."
      Mursula A.M., van Aalten D.M., Modis Y., Hiltunen J.K., Wierenga R.K.
      Acta Crystallogr. D 56:1020-1023(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION, SUBUNIT.
    6. "Eci1p uses a PTS1 to enter peroxisomes: either its own or that of a partner, Dci1p."
      Yang X., Purdue P.E., Lazarow P.B.
      Eur. J. Cell Biol. 80:126-138(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DOMAIN.
    7. "The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase."
      Mursula A.M., van Aalten D.M., Hiltunen J.K., Wierenga R.K.
      J. Mol. Biol. 309:845-853(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEX WITH OCTANOYL COENZYME A, MUTAGENESIS OF GLU-158, SUBUNIT.
    8. "Structural studies on delta(3)-delta(2)-enoyl-CoA isomerase: the variable mode of assembly of the trimeric disks of the crotonase superfamily."
      Mursula A.M., Hiltunen J.K., Wierenga R.K.
      FEBS Lett. 557:81-87(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiECI1_YEAST
    AccessioniPrimary (citable) accession number: Q05871
    Secondary accession number(s): D6VYS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3