ID   FOLC_BACSU              Reviewed;         430 AA.
AC   Q05865;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   16-JUN-2009, entry version 68.
DE   RecName: Full=Folylpolyglutamate synthase;
DE            EC=6.3.2.17;
DE   AltName: Full=Folylpoly-gamma-glutamate synthetase;
DE            Short=FPGS;
DE   AltName: Full=Tetrahydrofolate synthase;
DE   AltName: Full=Tetrahydrofolylpolyglutamate synthase;
GN   Name=folC; OrderedLocusNames=BSU28080;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / PY79;
RX   MEDLINE=93123172; PubMed=8419299;
RA   Margolis P.S., Driks A., Losick R.;
RT   "Sporulation gene spoIIB from Bacillus subtilis.";
RL   J. Bacteriol. 175:528-540(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 294-429.
RX   MEDLINE=90036690; PubMed=2553669;
RA   Mohan S., Aghion J., Guillen N., Dubnau D.A.;
RT   "Molecular cloning and characterization of comC, a late competence
RT   gene of Bacillus subtilis.";
RL   J. Bacteriol. 171:6043-6051(1989).
CC   -!- FUNCTION: Conversion of folates to polyglutamate derivatives. It
CC       preferes 5,10-methylenetetrahydrofolate, rather than 10-
CC       formyltetrahydrofolate as folate substrate.
CC   -!- CATALYTIC ACTIVITY: ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-
CC       glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolylpolyglutamate
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the folylpolyglutamate synthase family.
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DR   EMBL; L04520; AAB59021.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14768.1; -; Genomic_DNA.
DR   EMBL; M30805; AAA83364.1; ALT_INIT; Genomic_DNA.
DR   PIR; B40646; B40646.
DR   RefSeq; NP_390686.1; -.
DR   HSSP; P15925; 1FGS.
DR   GeneID; 936327; -.
DR   GenomeReviews; AL009126_GR; BSU28080.
DR   KEGG; bsu:BSU28080; -.
DR   NMPDR; fig|224308.1.peg.2811; -.
DR   SubtiList; BG10322; folC.
DR   HOGENOM; Q05865; -.
DR   OMA; Q05865; QRWPETK.
DR   BioCyc; BSUB224308:BSU2804-MON; -.
DR   BRENDA; 6.3.2.17; 150.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004326; F:tetrahydrofolylpolyglutamate synthase activity; IEA:EC.
DR   GO; GO:0009396; P:folic acid and derivative biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR018109; Folylpolyglutamate_synth_CS.
DR   InterPro; IPR001645; Fpolygl_synthtse.
DR   InterPro; IPR004101; Mur_ligase_C.
DR   InterPro; IPR013221; Mur_ligase_cen.
DR   Gene3D; G3DSA:3.90.190.20; Mur_ligase_C; 1.
DR   Gene3D; G3DSA:3.40.1190.10; Mur_ligase_cen; 1.
DR   PANTHER; PTHR11136; Fpolygl_synthtse; 1.
DR   Pfam; PF02875; Mur_ligase_C; 1.
DR   Pfam; PF08245; Mur_ligase_M; 1.
DR   TIGRFAMs; TIGR01499; folC; 1.
DR   PROSITE; PS01011; FOLYLPOLYGLU_SYNT_1; 1.
DR   PROSITE; PS01012; FOLYLPOLYGLU_SYNT_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; Nucleotide-binding;
KW   One-carbon metabolism.
FT   CHAIN         1    430       Folylpolyglutamate synthase.
FT                                /FTId=PRO_0000168299.
FT   NP_BIND      48     54       ATP (By similarity).
FT   CONFLICT    295    295       V -> M (in Ref. 2; AAA83364).
SQ   SEQUENCE   430 AA;  48165 MW;  8AD1DF9151D51160 CRC64;
     MFTAYQDARS WIHGRLKFGV KPGLGRMKQL MARLGHPEKK IRAFHVAGTN GKGSTVAFIR
     SMLQEAGYTV GTFTSPYIIT FNERISVNGI PISDEEWTAL VNQMKPHVEA LDQTEYGQPT
     EFEIMTACAF LYFAEFHKVD FVIFETGLGG RFDSTNVVEP LLTVITSIGH DHMNILGNTI
     EEIAGEKAGI IKEGIPIVTA VTQPEALQVI RHEAERHAAP FQSLHDACVI FNEEALPAGE
     QFSFKTEEKC YEDIRTSLIG THQRQNAALS ILAAEWLNKE NIAHISDEAL RSGLVKAAWP
     GRLELVQEHP PVYLDGAHNE EGVEKLAETM KQRFANSRIS VVFSALKDKP YQNMIKRLET
     IAHAIHFASF DFPRASLAKD LYDASEISNK SWSEDPDDVI KFIESKKGSN EIVLITGSLY
     FISDIRKRLK
//