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Protein

Diacylglycerol acyltransferase/mycolyltransferase Ag85C

Gene

fbpC

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria to fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei170NucleophileBy similarity1
Binding sitei170SubstrateBy similarity1
Binding sitei198SubstrateBy similarity1
Active sitei274By similarity1
Active sitei306By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Protein family/group databases

ESTHERimycle-a85c. A85-Mycolyl-transferase.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol acyltransferase/mycolyltransferase Ag85C (EC:2.3.1.122, EC:2.3.1.20)
Short name:
DGAT
Alternative name(s):
Acyl-CoA:diacylglycerol acyltransferase
Antigen 85 complex C
Short name:
85C
Short name:
Ag85C
Fibronectin-binding protein C
Short name:
Fbps C
Gene namesi
Name:fbpC
Synonyms:fbpC2
Ordered Locus Names:ML2655
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2655.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 44Sequence analysisAdd BLAST44
ChainiPRO_000000022545 – 333Diacylglycerol acyltransferase/mycolyltransferase Ag85CAdd BLAST289

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272631.ML2655.

Structurei

3D structure databases

ProteinModelPortaliQ05862.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 87Substrate bindingBy similarity2
Regioni102 – 112Fibronectin-bindingAdd BLAST11
Regioni276 – 279Substrate bindingBy similarity4
Regioni306 – 308SubstrateBy similarity3

Sequence similaritiesi

Belongs to the mycobacterial A85 antigen family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0627. LUCA.
HOGENOMiHOG000049505.
KOiK18851.
OMAiTRIWIYC.
OrthoDBiPOG091H0560.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLQQMRKL FGLAAKFPAR LTIAVIGTAL LAGLVGVVGD TAIAVAFSKP
60 70 80 90 100
GLPVEYLQVP SPSMGHDIKI QFQGGGQHAV YLLDGLRAQE DYNGWDINTP
110 120 130 140 150
AFEEYYHSGL SVIMPVGGQS SFYSNWYQPS QGNGQHYTYK WETFLTQEMP
160 170 180 190 200
SWLQANKNVL PTGNAAVGLS MSGSSALILA SYYPQQFPYA ASLSGFLNPS
210 220 230 240 250
EGWWPTMIGL AMNDSGGYNA NSMWGPSTDP AWKRNDPMVQ IPRLVANNTR
260 270 280 290 300
IWVYCGNGAP NELGGDNIPA KFLESLTLST NEIFQNTYAA SGGRNGVFNF
310 320 330
PPNGTHSWPY WNQQLVAMKP DIQQILNGSN NNA
Length:333
Mass (Da):36,392
Last modified:February 1, 1994 - v1
Checksum:i50EADF1F731E8EC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21951 Genomic DNA. Translation: CAA79949.1.
M90649 Genomic DNA. Translation: AAA91865.1.
AL583926 Genomic DNA. Translation: CAC32187.1.
PIRiS32114.
RefSeqiNP_302695.1. NC_002677.1.
WP_010909014.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC32187; CAC32187; CAC32187.
GeneIDi910842.
KEGGimle:ML2655.
PATRICi18060377. VBIMycLep78757_5102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21951 Genomic DNA. Translation: CAA79949.1.
M90649 Genomic DNA. Translation: AAA91865.1.
AL583926 Genomic DNA. Translation: CAC32187.1.
PIRiS32114.
RefSeqiNP_302695.1. NC_002677.1.
WP_010909014.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ05862.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2655.

Protein family/group databases

ESTHERimycle-a85c. A85-Mycolyl-transferase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC32187; CAC32187; CAC32187.
GeneIDi910842.
KEGGimle:ML2655.
PATRICi18060377. VBIMycLep78757_5102.

Organism-specific databases

LepromaiML2655.

Phylogenomic databases

eggNOGiCOG0627. LUCA.
HOGENOMiHOG000049505.
KOiK18851.
OMAiTRIWIYC.
OrthoDBiPOG091H0560.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA85C_MYCLE
AccessioniPrimary (citable) accession number: Q05862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 5, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.