Formin-1 - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Formin-1

Alternative name(s):
Limb deformity protein

Gene names

Name:	Fmn1
Synonyms:	Fmn, Ld

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1466 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the formation of adherens junction and the polymerization of linear actin cables. Ref.6 Ref.7
Subunit structure	Interacts with alpha-catenin and may interact with tubulin. Ref.7 Ref.8
Subcellular location	Nucleus. Cytoplasm. Cell junction › adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Localization to the adherens junctions is alpha-catenin-dependent. Also localizes to F-actin bundles originating from adherens junctions. Ref.7 Ref.8 Isoform 2: Cytoplasm › cytoskeleton. Note: Isoform 2 localizes to microtubules. Ref.7 Ref.8
Tissue specificity	It is present in the adult kidney, testis, limb, ovary, brain, small intestine, salivary gland and harderian gland. Isoforms 1, 2 and 5 are detected in skin and keratinocytes. Isoform 5 is found throughout the embryo. Ref.1 Ref.7
Developmental stage	It is present throughout the embryo. In the developing limb bud, the protein is expressed in the apical ectodermal ridge and the mesenchymal compartment, predominantly in the posterior region. During kidney morphogenesis, expression is initially restricted to the epithelial compartment of the pronephros and mesonephros. Isoform 5 is found in the apical ectodermal ridge and the mesenchymal compartment of the developing limb bud.
Post-translational modification	Phosphorylated on serine and possibly threonine residues. Ref.4
Miscellaneous	Was originally thought to play a role in limb bud development based on the fact that limb deformity (ld) mutants are associated with Fmn1 gene disruption. However, Ref.6 shows that limb deformity mutations rather affects Grem1 cis-regulatory regions localized in Fmn1 gene and that loss of Grem1 (gremlin-1) expression is the cause of limb malformations.
Sequence similarities	Belongs to the formin homology family. Cappuccino subfamily. Contains 1 FH1 (formin homology 1) domain. Contains 1 FH2 (formin homology 2) domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Cytoskeleton Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro forelimb morphogenesis Inferred from mutant phenotype PubMed 17554679. Source: MGI gene expression Inferred from mutant phenotype PubMed 17554679. Source: MGI hindlimb morphogenesis Inferred from mutant phenotype PubMed 17554679. Source: MGI positive regulation of actin filament polymerization Inferred from direct assay Ref.7. Source: MGI positive regulation of actin nucleation Inferred from direct assay Ref.7. Source: MGI skeletal system morphogenesis Inferred from mutant phenotype PubMed 17554679. Source: MGI
Cellular_component	actin cytoskeleton Inferred from electronic annotation. Source: InterPro adherens junction Inferred from physical interaction Ref.7. Source: MGI cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay Ref.4. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q05860-1) Also known as: IA; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q05860-2) Also known as: IB; The sequence of this isoform differs from the canonical sequence as follows: 1250-1285: Missing.

Isoform 3 (identifier: Q05860-3) Also known as: II; The sequence of this isoform differs from the canonical sequence as follows: 625-722: Missing.

Isoform 4 (identifier: Q05860-4) Also known as: III; The sequence of this isoform differs from the canonical sequence as follows: 626-627: IA → SV 628-1466: Missing.

Isoform 5 (identifier: Q05860-5) Also known as: IV; The sequence of this isoform differs from the canonical sequence as follows: 1-682: Missing. 683-683: N → MEEVGNSLSS...PADSPSDPKS 1250-1285: Missing.

Isoform 6 (identifier: Q05860-6) Also known as: V; The sequence of this isoform differs from the canonical sequence as follows: 1-623: Missing. 624-624: P → MVTTGSPPFNTMGACYRYNPRYGQPISVPKVWKCRHRRSVTPDE 1250-1285: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1466

1466

Formin-1

PRO_0000194886

Regions

Domain

870 – 970

101

FH1

Domain

983 – 1435

453

FH2

Region

1 – 624

624

Microtubule-binding

Region

458 – 842

385

Mediates interaction with alpha-catenin

Coiled coil

722 – 786

65

Potential

Compositional bias

870 – 995

126

Pro-rich

Amino acid modifications

Modified residue

234

1

Phosphoserine Ref.9

Natural variations

Alternative sequence

1 – 682

682

Missing in isoform 5.

VSP_027214

Alternative sequence

1 – 623

623

Missing in isoform 6.

VSP_029424

Alternative sequence

624

1

P → MVTTGSPPFNTMGACYRYNP RYGQPISVPKVWKCRHRRSV TPDE in isoform 6.

VSP_029425

Alternative sequence

625 – 722

98

Missing in isoform 3.

VSP_001570

Alternative sequence

626 – 627

2

IA → SV in isoform 4.

VSP_001571

Alternative sequence

628 – 1466

839

Missing in isoform 4.

VSP_001572

Alternative sequence

683

1

N → MEEVGNSLSSRDVLEPDKSE AGLEMAQSILSKFSMKSLFG FTNKLDSLEPEEEDAVLKAF RSLEGDPAPERGDPSKGSDQ PQAEAPVPPDLKNDGKSARA ETGSEGSQGKGRSNTSSPGY ELSPATVSVDNEEVIWVRGT LVHTTSDSDSEDGDQEAEEE SSLDTQKPTTVVLCEPSQEP KDRAGDSEENTDTGNTDDTE LCAEESQRTLPETSSKLELG GDGSHPAEHSPRQDQAAEEG SQIPPAATDQTVGALASTVS KREAPEEKPFQLPAFFSGLR VLKKGATAEAGETITEIKPK DGDLALLKLTQRVQKSLGQG GPQTVKSPGRATDPKATPTL LEQLSQLLNIDMPRTEQKEA DPEFHGADEMGYSTDQESHK SPRDAHVQGGQVKARTPETA LEAFKALFIRPPKKGSTADT SELEALKRKMKHEKESLRAV FERSKSRPADSPSDPKS in isoform 5.

VSP_027215

Alternative sequence

1250 – 1285

36

Missing in isoform 2, isoform 5 and isoform 6.

VSP_027216

Experimental info

Sequence conflict

220

1

V → A in CAA37668. Ref.1

Sequence conflict

776

1

G → E in CAA37668. Ref.1

Sequence conflict

776

1

G → E in CAA44244. Ref.2

Sequence conflict

866

1

H → Q in CAA37668. Ref.1

Sequence conflict

866

1

H → Q in CAA44244. Ref.2

Sequence conflict

950

1

G → GPP in CAA37668. Ref.1

Sequence conflict

950

1

G → GPP in CAA44244. Ref.2

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (IA) [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 5482F8AAB66CBF32 Show »	FASTA	1,466	163,581
10 20 30 40 50 60 MEGTHCTLQL HNPIAELCYI SFYLPKGEVR GFSYKGTVTL DRSNNAFHNC YQVREGPDIT 70 80 90 100 110 120 SLSQQPNEHP GDIFFKQTPT KNILTELYKL TAEKERLLDS LLRSDNILGV SMGSQEGKLQ 130 140 150 160 170 180 ELSVILATGD EYFQSAGNWR RELPVSSLIR RSTQENKKPR RSGRRRESPE ELRQKRTRRK 190 200 210 220 230 240 GRGCQESAFQ MGKDQVCSSS SLSFRARPNL RLLEERGNLV PRGTLTSSLR RRESCPANIL 250 260 270 280 290 300 RTPDADLAFG NSGRTSEDTD LEGPLSPDSS PTEVGDADVG GQLKSSHQQE PPQPNVSESH 310 320 330 340 350 360 GKHAGAERWS SRTRKSKSLE RTCSKKPVSK VVAKIQEPSA PVKRIVRAHH DGKGRVAYGP 370 380 390 400 410 420 ETQTEFIPKA DFLTLPGGET ETHSSGRLEE EQPGIKSLRS SAPERASITK EPASTEAAVN 430 440 450 460 470 480 KVLRKVIESE KLDEATEGKR LGFSLNTRAT HTFPETRSQR KAGLPQSGHK FLLLDLPHTV 490 500 510 520 530 540 GPDSPQPKCD EKKPTPQVPT ALGMVFNNSS PQSSAHKRLS PVPSPLSPRC PSPQQHHRIL 550 560 570 580 590 600 LLPPLPSEGE VVFNEYPSRK NDVSSGFPSA DTLEPSSTTK VTETKGASPT SLRASQTWLV 610 620 630 640 650 660 SEEASEKGLG PEKITAPPQH QLPPGIASEG FPCDNFKEQT AKDLPNKDGG VWVPGYRAGP 670 680 690 700 710 720 PCPFLLHEEK EKTSRSELYL DLNPDQSPTE QDDRTPGRLQ AVWPPPKTKD TEEKVGLKYT 730 740 750 760 770 780 EAEYQAAILH LKREHKEEIE TLQAQFELKT FHIRGEHALV TARLEEAIEN LKQQLGKRRE 790 800 810 820 830 840 GCEEMRDVCI STDDDCSPKA FRNVCIQTDR ETFLKPCDAE SKATRSSQIV PKKLTISLTQ 850 860 870 880 890 900 LSPSKDSKDI HAPFQTREGT SSSSQHKISP PAPPTPPPLP PPLIPPPPPL PPGLGPLPPA 910 920 930 940 950 960 PPIPPVCPVS PPPPPPPPPP TPVPPSDGPP PPPPPPPPLP NVLALPNSGG PPPPPPPPPP 970 980 990 1000 1010 1020 GLAPPPPPGL SFGLSSSSSQ YPRKPAIEPS CPMKPLYWTR IQINDKSQDA APTLWDSLEE 1030 1040 1050 1060 1070 1080 PHIRDTSEFE YLFSKDTTQQ KKKPLSEAYE KKNKVKKIIK LLDGKRSQTV GILISSLHLE 1090 1100 1110 1120 1130 1140 MKDIQQAIFT VDDSVVDLET LAALYENRAQ EDELTKIRKY YETSKEEDLK LLDKPEQFLH 1150 1160 1170 1180 1190 1200 ELAQIPNFAE RAQCIIFRAV FSEGITSLHR KVEIVTRASK GLLHMKSVKD ILALILAFGN 1210 1220 1230 1240 1250 1260 YMNGGNRTRG QADGYSLEIL PKLKDVKSRD NGMNLVDYVV KYYLRYYDQC KHHDQEASCR 1270 1280 1290 1300 1310 1320 GKDLFSLYFH IAVHPQRKSG LELKQEAGTD KSVFPLPEPQ DFFLASQVKF EDLLKDLRKL 1330 1340 1350 1360 1370 1380 KRQLEASEQQ MKLVCKESPR EYLQPFKDKL EEFFKKAKKE HKMEESHLEN AQKSFETTVG 1390 1400 1410 1420 1430 1440 YFGMKPKTGE KEVTPSYVFM VWFEFCSDFK TIWKRESKNI SKERLKMAQA SVSKLTSEKK 1450 1460 VETKKINPTA SLKERLRQKE ASVATN « Hide
Isoform 2 (IB) [UniParc]. Checksum: 6A90287624580946 Show »	FASTA	1,430	159,391
10 20 30 40 50 60 MEGTHCTLQL HNPIAELCYI SFYLPKGEVR GFSYKGTVTL DRSNNAFHNC YQVREGPDIT 70 80 90 100 110 120 SLSQQPNEHP GDIFFKQTPT KNILTELYKL TAEKERLLDS LLRSDNILGV SMGSQEGKLQ 130 140 150 160 170 180 ELSVILATGD EYFQSAGNWR RELPVSSLIR RSTQENKKPR RSGRRRESPE ELRQKRTRRK 190 200 210 220 230 240 GRGCQESAFQ MGKDQVCSSS SLSFRARPNL RLLEERGNLV PRGTLTSSLR RRESCPANIL 250 260 270 280 290 300 RTPDADLAFG NSGRTSEDTD LEGPLSPDSS PTEVGDADVG GQLKSSHQQE PPQPNVSESH 310 320 330 340 350 360 GKHAGAERWS SRTRKSKSLE RTCSKKPVSK VVAKIQEPSA PVKRIVRAHH DGKGRVAYGP 370 380 390 400 410 420 ETQTEFIPKA DFLTLPGGET ETHSSGRLEE EQPGIKSLRS SAPERASITK EPASTEAAVN 430 440 450 460 470 480 KVLRKVIESE KLDEATEGKR LGFSLNTRAT HTFPETRSQR KAGLPQSGHK FLLLDLPHTV 490 500 510 520 530 540 GPDSPQPKCD EKKPTPQVPT ALGMVFNNSS PQSSAHKRLS PVPSPLSPRC PSPQQHHRIL 550 560 570 580 590 600 LLPPLPSEGE VVFNEYPSRK NDVSSGFPSA DTLEPSSTTK VTETKGASPT SLRASQTWLV 610 620 630 640 650 660 SEEASEKGLG PEKITAPPQH QLPPGIASEG FPCDNFKEQT AKDLPNKDGG VWVPGYRAGP 670 680 690 700 710 720 PCPFLLHEEK EKTSRSELYL DLNPDQSPTE QDDRTPGRLQ AVWPPPKTKD TEEKVGLKYT 730 740 750 760 770 780 EAEYQAAILH LKREHKEEIE TLQAQFELKT FHIRGEHALV TARLEEAIEN LKQQLGKRRE 790 800 810 820 830 840 GCEEMRDVCI STDDDCSPKA FRNVCIQTDR ETFLKPCDAE SKATRSSQIV PKKLTISLTQ 850 860 870 880 890 900 LSPSKDSKDI HAPFQTREGT SSSSQHKISP PAPPTPPPLP PPLIPPPPPL PPGLGPLPPA 910 920 930 940 950 960 PPIPPVCPVS PPPPPPPPPP TPVPPSDGPP PPPPPPPPLP NVLALPNSGG PPPPPPPPPP 970 980 990 1000 1010 1020 GLAPPPPPGL SFGLSSSSSQ YPRKPAIEPS CPMKPLYWTR IQINDKSQDA APTLWDSLEE 1030 1040 1050 1060 1070 1080 PHIRDTSEFE YLFSKDTTQQ KKKPLSEAYE KKNKVKKIIK LLDGKRSQTV GILISSLHLE 1090 1100 1110 1120 1130 1140 MKDIQQAIFT VDDSVVDLET LAALYENRAQ EDELTKIRKY YETSKEEDLK LLDKPEQFLH 1150 1160 1170 1180 1190 1200 ELAQIPNFAE RAQCIIFRAV FSEGITSLHR KVEIVTRASK GLLHMKSVKD ILALILAFGN 1210 1220 1230 1240 1250 1260 YMNGGNRTRG QADGYSLEIL PKLKDVKSRD NGMNLVDYVV KYYLRYYDQE AGTDKSVFPL 1270 1280 1290 1300 1310 1320 PEPQDFFLAS QVKFEDLLKD LRKLKRQLEA SEQQMKLVCK ESPREYLQPF KDKLEEFFKK 1330 1340 1350 1360 1370 1380 AKKEHKMEES HLENAQKSFE TTVGYFGMKP KTGEKEVTPS YVFMVWFEFC SDFKTIWKRE 1390 1400 1410 1420 1430 SKNISKERLK MAQASVSKLT SEKKVETKKI NPTASLKERL RQKEASVATN « Hide
Isoform 3 (II) [UniParc]. Checksum: F34DEB5CF39489AA Show »	FASTA	1,368	152,641
10 20 30 40 50 60 MEGTHCTLQL HNPIAELCYI SFYLPKGEVR GFSYKGTVTL DRSNNAFHNC YQVREGPDIT 70 80 90 100 110 120 SLSQQPNEHP GDIFFKQTPT KNILTELYKL TAEKERLLDS LLRSDNILGV SMGSQEGKLQ 130 140 150 160 170 180 ELSVILATGD EYFQSAGNWR RELPVSSLIR RSTQENKKPR RSGRRRESPE ELRQKRTRRK 190 200 210 220 230 240 GRGCQESAFQ MGKDQVCSSS SLSFRARPNL RLLEERGNLV PRGTLTSSLR RRESCPANIL 250 260 270 280 290 300 RTPDADLAFG NSGRTSEDTD LEGPLSPDSS PTEVGDADVG GQLKSSHQQE PPQPNVSESH 310 320 330 340 350 360 GKHAGAERWS SRTRKSKSLE RTCSKKPVSK VVAKIQEPSA PVKRIVRAHH DGKGRVAYGP 370 380 390 400 410 420 ETQTEFIPKA DFLTLPGGET ETHSSGRLEE EQPGIKSLRS SAPERASITK EPASTEAAVN 430 440 450 460 470 480 KVLRKVIESE KLDEATEGKR LGFSLNTRAT HTFPETRSQR KAGLPQSGHK FLLLDLPHTV 490 500 510 520 530 540 GPDSPQPKCD EKKPTPQVPT ALGMVFNNSS PQSSAHKRLS PVPSPLSPRC PSPQQHHRIL 550 560 570 580 590 600 LLPPLPSEGE VVFNEYPSRK NDVSSGFPSA DTLEPSSTTK VTETKGASPT SLRASQTWLV 610 620 630 640 650 660 SEEASEKGLG PEKITAPPQH QLPPEYQAAI LHLKREHKEE IETLQAQFEL KTFHIRGEHA 670 680 690 700 710 720 LVTARLEEAI ENLKQQLGKR REGCEEMRDV CISTDDDCSP KAFRNVCIQT DRETFLKPCD 730 740 750 760 770 780 AESKATRSSQ IVPKKLTISL TQLSPSKDSK DIHAPFQTRE GTSSSSQHKI SPPAPPTPPP 790 800 810 820 830 840 LPPPLIPPPP PLPPGLGPLP PAPPIPPVCP VSPPPPPPPP PPTPVPPSDG PPPPPPPPPP 850 860 870 880 890 900 LPNVLALPNS GGPPPPPPPP PPGLAPPPPP GLSFGLSSSS SQYPRKPAIE PSCPMKPLYW 910 920 930 940 950 960 TRIQINDKSQ DAAPTLWDSL EEPHIRDTSE FEYLFSKDTT QQKKKPLSEA YEKKNKVKKI 970 980 990 1000 1010 1020 IKLLDGKRSQ TVGILISSLH LEMKDIQQAI FTVDDSVVDL ETLAALYENR AQEDELTKIR 1030 1040 1050 1060 1070 1080 KYYETSKEED LKLLDKPEQF LHELAQIPNF AERAQCIIFR AVFSEGITSL HRKVEIVTRA 1090 1100 1110 1120 1130 1140 SKGLLHMKSV KDILALILAF GNYMNGGNRT RGQADGYSLE ILPKLKDVKS RDNGMNLVDY 1150 1160 1170 1180 1190 1200 VVKYYLRYYD QCKHHDQEAS CRGKDLFSLY FHIAVHPQRK SGLELKQEAG TDKSVFPLPE 1210 1220 1230 1240 1250 1260 PQDFFLASQV KFEDLLKDLR KLKRQLEASE QQMKLVCKES PREYLQPFKD KLEEFFKKAK 1270 1280 1290 1300 1310 1320 KEHKMEESHL ENAQKSFETT VGYFGMKPKT GEKEVTPSYV FMVWFEFCSD FKTIWKRESK 1330 1340 1350 1360 NISKERLKMA QASVSKLTSE KKVETKKINP TASLKERLRQ KEASVATN « Hide
Isoform 4 (III) [UniParc]. Checksum: F25AF953C733E24F Show »	FASTA	627	68,996
10 20 30 40 50 60 MEGTHCTLQL HNPIAELCYI SFYLPKGEVR GFSYKGTVTL DRSNNAFHNC YQVREGPDIT 70 80 90 100 110 120 SLSQQPNEHP GDIFFKQTPT KNILTELYKL TAEKERLLDS LLRSDNILGV SMGSQEGKLQ 130 140 150 160 170 180 ELSVILATGD EYFQSAGNWR RELPVSSLIR RSTQENKKPR RSGRRRESPE ELRQKRTRRK 190 200 210 220 230 240 GRGCQESAFQ MGKDQVCSSS SLSFRARPNL RLLEERGNLV PRGTLTSSLR RRESCPANIL 250 260 270 280 290 300 RTPDADLAFG NSGRTSEDTD LEGPLSPDSS PTEVGDADVG GQLKSSHQQE PPQPNVSESH 310 320 330 340 350 360 GKHAGAERWS SRTRKSKSLE RTCSKKPVSK VVAKIQEPSA PVKRIVRAHH DGKGRVAYGP 370 380 390 400 410 420 ETQTEFIPKA DFLTLPGGET ETHSSGRLEE EQPGIKSLRS SAPERASITK EPASTEAAVN 430 440 450 460 470 480 KVLRKVIESE KLDEATEGKR LGFSLNTRAT HTFPETRSQR KAGLPQSGHK FLLLDLPHTV 490 500 510 520 530 540 GPDSPQPKCD EKKPTPQVPT ALGMVFNNSS PQSSAHKRLS PVPSPLSPRC PSPQQHHRIL 550 560 570 580 590 600 LLPPLPSEGE VVFNEYPSRK NDVSSGFPSA DTLEPSSTTK VTETKGASPT SLRASQTWLV 610 620 SEEASEKGLG PEKITAPPQH QLPPGSV « Hide
Isoform 5 (IV) [UniParc]. Checksum: 8724170303A83D33 Show »	FASTA	1,204	133,207
10 20 30 40 50 60 MEEVGNSLSS RDVLEPDKSE AGLEMAQSIL SKFSMKSLFG FTNKLDSLEP EEEDAVLKAF 70 80 90 100 110 120 RSLEGDPAPE RGDPSKGSDQ PQAEAPVPPD LKNDGKSARA ETGSEGSQGK GRSNTSSPGY 130 140 150 160 170 180 ELSPATVSVD NEEVIWVRGT LVHTTSDSDS EDGDQEAEEE SSLDTQKPTT VVLCEPSQEP 190 200 210 220 230 240 KDRAGDSEEN TDTGNTDDTE LCAEESQRTL PETSSKLELG GDGSHPAEHS PRQDQAAEEG 250 260 270 280 290 300 SQIPPAATDQ TVGALASTVS KREAPEEKPF QLPAFFSGLR VLKKGATAEA GETITEIKPK 310 320 330 340 350 360 DGDLALLKLT QRVQKSLGQG GPQTVKSPGR ATDPKATPTL LEQLSQLLNI DMPRTEQKEA 370 380 390 400 410 420 DPEFHGADEM GYSTDQESHK SPRDAHVQGG QVKARTPETA LEAFKALFIR PPKKGSTADT 430 440 450 460 470 480 SELEALKRKM KHEKESLRAV FERSKSRPAD SPSDPKSPDQ SPTEQDDRTP GRLQAVWPPP 490 500 510 520 530 540 KTKDTEEKVG LKYTEAEYQA AILHLKREHK EEIETLQAQF ELKTFHIRGE HALVTARLEE 550 560 570 580 590 600 AIENLKQQLG KRREGCEEMR DVCISTDDDC SPKAFRNVCI QTDRETFLKP CDAESKATRS 610 620 630 640 650 660 SQIVPKKLTI SLTQLSPSKD SKDIHAPFQT REGTSSSSQH KISPPAPPTP PPLPPPLIPP 670 680 690 700 710 720 PPPLPPGLGP LPPAPPIPPV CPVSPPPPPP PPPPTPVPPS DGPPPPPPPP PPLPNVLALP 730 740 750 760 770 780 NSGGPPPPPP PPPPGLAPPP PPGLSFGLSS SSSQYPRKPA IEPSCPMKPL YWTRIQINDK 790 800 810 820 830 840 SQDAAPTLWD SLEEPHIRDT SEFEYLFSKD TTQQKKKPLS EAYEKKNKVK KIIKLLDGKR 850 860 870 880 890 900 SQTVGILISS LHLEMKDIQQ AIFTVDDSVV DLETLAALYE NRAQEDELTK IRKYYETSKE 910 920 930 940 950 960 EDLKLLDKPE QFLHELAQIP NFAERAQCII FRAVFSEGIT SLHRKVEIVT RASKGLLHMK 970 980 990 1000 1010 1020 SVKDILALIL AFGNYMNGGN RTRGQADGYS LEILPKLKDV KSRDNGMNLV DYVVKYYLRY 1030 1040 1050 1060 1070 1080 YDQEAGTDKS VFPLPEPQDF FLASQVKFED LLKDLRKLKR QLEASEQQMK LVCKESPREY 1090 1100 1110 1120 1130 1140 LQPFKDKLEE FFKKAKKEHK MEESHLENAQ KSFETTVGYF GMKPKTGEKE VTPSYVFMVW 1150 1160 1170 1180 1190 1200 FEFCSDFKTI WKRESKNISK ERLKMAQASV SKLTSEKKVE TKKINPTASL KERLRQKEAS VATN « Hide
Isoform 6 (V) [UniParc]. Checksum: 173EC42FD49F66D9 Show »	FASTA	850	95,723
10 20 30 40 50 60 MVTTGSPPFN TMGACYRYNP RYGQPISVPK VWKCRHRRSV TPDEGIASEG FPCDNFKEQT 70 80 90 100 110 120 AKDLPNKDGG VWVPGYRAGP PCPFLLHEEK EKTSRSELYL DLNPDQSPTE QDDRTPGRLQ 130 140 150 160 170 180 AVWPPPKTKD TEEKVGLKYT EAEYQAAILH LKREHKEEIE TLQAQFELKT FHIRGEHALV 190 200 210 220 230 240 TARLEEAIEN LKQQLGKRRE GCEEMRDVCI STDDDCSPKA FRNVCIQTDR ETFLKPCDAE 250 260 270 280 290 300 SKATRSSQIV PKKLTISLTQ LSPSKDSKDI HAPFQTREGT SSSSQHKISP PAPPTPPPLP 310 320 330 340 350 360 PPLIPPPPPL PPGLGPLPPA PPIPPVCPVS PPPPPPPPPP TPVPPSDGPP PPPPPPPPLP 370 380 390 400 410 420 NVLALPNSGG PPPPPPPPPP GLAPPPPPGL SFGLSSSSSQ YPRKPAIEPS CPMKPLYWTR 430 440 450 460 470 480 IQINDKSQDA APTLWDSLEE PHIRDTSEFE YLFSKDTTQQ KKKPLSEAYE KKNKVKKIIK 490 500 510 520 530 540 LLDGKRSQTV GILISSLHLE MKDIQQAIFT VDDSVVDLET LAALYENRAQ EDELTKIRKY 550 560 570 580 590 600 YETSKEEDLK LLDKPEQFLH ELAQIPNFAE RAQCIIFRAV FSEGITSLHR KVEIVTRASK 610 620 630 640 650 660 GLLHMKSVKD ILALILAFGN YMNGGNRTRG QADGYSLEIL PKLKDVKSRD NGMNLVDYVV 670 680 690 700 710 720 KYYLRYYDQE AGTDKSVFPL PEPQDFFLAS QVKFEDLLKD LRKLKRQLEA SEQQMKLVCK 730 740 750 760 770 780 ESPREYLQPF KDKLEEFFKK AKKEHKMEES HLENAQKSFE TTVGYFGMKP KTGEKEVTPS 790 800 810 820 830 840 YVFMVWFEFC SDFKTIWKRE SKNISKERLK MAQASVSKLT SEKKVETKKI NPTASLKERL 850 RQKEASVATN « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"'Formins': proteins deduced from the alternative transcripts of the limb deformity gene." Woychik R.P., Maas R.L., Zeller R., Vogt T.F., Leder P. Nature 346:850-853(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Tissue: Kidney and Testis.
[2]	"A variant limb deformity transcript expressed in the embryonic mouse limb defines a novel formin." Grusby-Jackson L., Kuo A., Leder P. Genes Dev. 6:29-37(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), ALTERNATIVE SPLICING. Tissue: Embryo.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"Formins: phosphoprotein isoforms encoded by the mouse limb deformity locus." Vogt T.F., Jackson-Grusby L., Rush J., Leder P. Proc. Natl. Acad. Sci. U.S.A. 90:5554-5558(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, ALTERNATIVE SPLICING (ISOFORMS 1; 2; 3; 4 AND 5).
[5]	"The mouse formin (Fmn) gene: genomic structure, novel exons, and genetic mapping." Wang C.C., Chan D.C., Leder P. Genomics 39:303-311(1997) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 2; 3; 4 AND 5).
[6]	"Mouse limb deformity mutations disrupt a global control region within the large regulatory landscape required for Gremlin expression." Zuniga A., Michos O., Spitz F., Haramis A.-P.G., Panman L., Galli A., Vintersten K., Klasen C., Mansfield W., Kuc S., Duboule D., Dono R., Zeller R. Genes Dev. 18:1553-1564(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Mammalian formin-1 participates in adherens junctions and polymerization of linear actin cables." Kobielak A., Pasolli H.A., Fuchs E. Nat. Cell Biol. 6:21-30(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 5 AND 6), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ALPHA-CATENIN, TISSUE SPECIFICITY.
[8]	"Formin-1 protein associates with microtubules through a peptide domain encoded by exon-2." Zhou F., Leder P., Martin S.S. Exp. Cell Res. 312:1119-1126(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TUBULIN.
[9]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X53599 mRNA. Translation: CAA37668.1.
X62379 mRNA. Translation: CAA44244.1.
AL672253, AL691437 Genomic DNA. Translation: CAM14988.1.
AL672253, AL691437, AL691466 Genomic DNA. Translation: CAM14989.1.
AL691466, AL672253, AL691437 Genomic DNA. Translation: CAM18143.1.
AL691437, AL672253 Genomic DNA. Translation: CAM18583.1.
AL691437, AL672253, AL691466 Genomic DNA. Translation: CAM18585.1.

IPI

IPI00114260.
IPI00229709.
IPI00229710.
IPI00828236.
IPI00828355.
IPI00874986.

PIR

S11515.
S24407.

RefSeq

NP_001036787.1. NM_001043322.1.
NP_034360.2. NM_010230.2.

UniGene

Mm.4938.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JUP	NMR	-	P	881-888	[»]
2RLY	NMR	-	P	874-881	[»]
2RM0	NMR	-	P	880-888	[»]

ProteinModelPortal

Q05860.

SMR

Q05860. Positions 970-1424.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-646N.

PTM databases

PhosphoSite

Q05860.

Proteomic databases

PaxDb

Q05860.

PRIDE

Q05860.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000081349; ENSMUSP00000080093; ENSMUSG00000044042.
ENSMUST00000099576; ENSMUSP00000097171; ENSMUSG00000044042.
ENSMUST00000102547; ENSMUSP00000099606; ENSMUSG00000044042.

GeneID

14260.

KEGG

mmu:14260.

UCSC

uc008lpl.1. mouse.
uc008lpm.1. mouse.
uc008lpn.1. mouse.
uc012cbb.1. mouse.

Organism-specific databases

CTD

342184.

MGI

MGI:101815. Fmn1.

Phylogenomic databases

eggNOG

NOG264408.

GeneTree

ENSGT00700000104245.

HOGENOM

HOG000168579.

HOVERGEN

HBG107922.

InParanoid

Q05860.

KO

K10367.

OMA

DSKSPDH.

OrthoDB

EOG4KWJSD.

Gene expression databases

ArrayExpress

Q05860.

Bgee

Q05860.

CleanEx

MM_FMN1.

Genevestigator

Q05860.

Family and domain databases

InterPro

IPR003104. Actin-bd_FH2/DRF_autoreg.
IPR015425. FH2_actin-bd.
IPR001265. Formin.
[Graphical view]

Pfam

PF02181. FH2. 1 hit.
[Graphical view]

PRINTS

PR00828. FORMIN.

SMART

SM00498. FH2. 1 hit.
[Graphical view]

SUPFAM

SSF101447. FH2_actin_bd. 1 hit.

PROSITE

PS51444. FH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q05860.

NextBio

285597.

SOURCE

Search...

Entry information

Entry name

FMN1_MOUSE

Accession

Primary (citable) accession number: Q05860
Secondary accession number(s): A2AFY9, A2AFZ0, Q05859

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families