Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q05852 (GTAB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UTP--glucose-1-phosphate uridylyltransferase

EC=2.7.7.9
Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
General stress protein 33
Short name=GSP33
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase
Gene names
Name:gtaB
Ordered Locus Names:BSU35670
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP. This is an intermediate step in the biosynthesis of diglucosyl-diacylglycerol (Glc2-DAG), i.e. the predominant glycolipid found in B.subtilis membrane, which is also used as a membrane anchor for lipoteichoic acid (LTA). Has a role in the biosynthesis of all phosphate-containing envelope polymers, since UDP-glucose serves as a glucosyl donor not only for the biosynthesis of LTA but also for wall teichoic acids (WTAs). Is required for biofilm formation. This is likely due to another role of UDP-glucose, which might also act as a metabolic signal regulating biofilm formation or may be involved in some unknown biosynthetic pathway essential for biofilm formation, e.g. the synthesis of an exopolysaccharide. Ref.1 Ref.2 Ref.5 Ref.6

Catalytic activity

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Pathway

Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis. Ref.6

Induction

By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. Expressed under control of the sigma-B transcription factor. Ref.1

Sequence similarities

Belongs to the UDPGP type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292UTP--glucose-1-phosphate uridylyltransferase
PRO_0000201364

Sequences

Sequence LengthMass (Da)Tools
Q05852 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: DFA640840826596F

FASTA29233,070
        10         20         30         40         50         60 
MKKVRKAIIP AAGLGTRFLP ATKAMPKEML PIVDKPTIQY IIEEAVEAGI EDIIIVTGKS 

        70         80         90        100        110        120 
KRAIEDHFDY SPELERNLEE KGKTELLEKV KKASNLADIH YIRQKEPKGL GHAVWCARNF 

       130        140        150        160        170        180 
IGDEPFAVLL GDDIVQAETP GLRQLMDEYE KTLSSIIGVQ QVPEEETHRY GIIDPLTSEG 

       190        200        210        220        230        240 
RRYQVKNFVE KPPKGTAPSN LAILGRYVFT PEIFMYLEEQ QVGAGGEIQL TDAIQKLNEI 

       250        260        270        280        290 
QRVFAYDFEG KRYDVGEKLG FITTTLEFAM QDKELRDQLV PFMEGLLNKE EI 

« Hide

References

« Hide 'large scale' references
[1]"Bacillus subtilis gtaB encodes UDP-glucose pyrophosphorylase and is controlled by stationary-phase transcription factor sigma B."
Varon D., Boylan S.A., Okamoto K., Price C.W.
J. Bacteriol. 175:3964-3971(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, ROLE IN GLYCOSYLATION OF WTAS.
Strain: 168.
[2]"Sequencing and analysis of the divergon comprising gtaB, the structural gene of UDP-glucose pyrophosphorylase of Bacillus subtilis 168."
Soldo B., Lazarevic V., Margot P., Karamata D.
J. Gen. Microbiol. 139:3185-3195(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
Electrophoresis 18:1451-1463(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-21.
Strain: 168 / IS58.
[5]"The gtaB marker in Bacillus subtilis 168 is associated with a deficiency in UDPglucose pyrophosphorylase."
Pooley H.M., Paschoud D., Karamata D.
J. Gen. Microbiol. 133:3481-3493(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168.
[6]"Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation."
Lazarevic V., Soldo B., Medico N., Pooley H.M., Bron S., Karamata D.
Appl. Environ. Microbiol. 71:39-45(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN BIOFILM FORMATION, PATHWAY.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12272 Unassigned DNA. Translation: AAA71967.1.
Z22516 Genomic DNA. Translation: CAA80241.1.
AL009126 Genomic DNA. Translation: CAB15584.1.
PIRA40650.
RefSeqNP_391447.1. NC_000964.3.

3D structure databases

ProteinModelPortalQ05852.
SMRQ05852. Positions 1-285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ05852. 1 interaction.
MINTMINT-8365569.
STRING224308.BSU35670.

Proteomic databases

PaxDbQ05852.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15584; CAB15584; BSU35670.
GeneID936797.
KEGGbsu:BSU35670.
PATRIC18979144. VBIBacSub10457_3734.

Organism-specific databases

GenoListBSU35670. [Micado]

Phylogenomic databases

eggNOGCOG1210.
HOGENOMHOG000283477.
KOK00963.
OMAKTHASIL.
OrthoDBEOG6Z9B3V.
ProtClustDBCLSK2518282.

Enzyme and pathway databases

BioCycBSUB:BSU35670-MONOMER.
MetaCyc:MONOMER-8824.
UniPathwayUPA00894.

Family and domain databases

InterProIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
[Graphical view]
PfamPF00483. NTP_transferase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01099. galU. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGTAB_BACSU
AccessionPrimary (citable) accession number: Q05852
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 13, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList