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Q05852

- GTAB_BACSU

UniProt

Q05852 - GTAB_BACSU

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Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

gtaB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP. This is an intermediate step in the biosynthesis of diglucosyl-diacylglycerol (Glc2-DAG), i.e. the predominant glycolipid found in B.subtilis membrane, which is also used as a membrane anchor for lipoteichoic acid (LTA). Has a role in the biosynthesis of all phosphate-containing envelope polymers, since UDP-glucose serves as a glucosyl donor not only for the biosynthesis of LTA but also for wall teichoic acids (WTAs). Is required for biofilm formation. This is likely due to another role of UDP-glucose, which might also act as a metabolic signal regulating biofilm formation or may be involved in some unknown biosynthetic pathway essential for biofilm formation, e.g. the synthesis of an exopolysaccharide.4 Publications

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Pathwayi

GO - Molecular functioni

  1. UTP:glucose-1-phosphate uridylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. enterobacterial common antigen biosynthetic process Source: UniProtKB-UniPathway
  2. response to stress Source: UniProtKB-KW
  3. UDP-glucose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Stress response

Enzyme and pathway databases

BioCyciBSUB:BSU35670-MONOMER.
MetaCyc:MONOMER-8824.
UniPathwayiUPA00894.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
General stress protein 33
Short name:
GSP33
UDP-glucose pyrophosphorylase
Short name:
UDPGP
Uridine diphosphoglucose pyrophosphorylase
Gene namesi
Name:gtaB
Ordered Locus Names:BSU35670
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU35670. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292UTP--glucose-1-phosphate uridylyltransferasePRO_0000201364Add
BLAST

Proteomic databases

PaxDbiQ05852.

Expressioni

Inductioni

By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. Expressed under control of the sigma-B transcription factor.1 Publication

Interactioni

Protein-protein interaction databases

IntActiQ05852. 1 interaction.
MINTiMINT-8365569.
STRINGi224308.BSU35670.

Structurei

3D structure databases

ProteinModelPortaliQ05852.
SMRiQ05852. Positions 1-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDPGP type 2 family.Curated

Phylogenomic databases

eggNOGiCOG1210.
HOGENOMiHOG000283477.
InParanoidiQ05852.
KOiK00963.
OMAiMDEIMTN.
OrthoDBiEOG6Z9B3V.
PhylomeDBiQ05852.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01099. galU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05852-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKVRKAIIP AAGLGTRFLP ATKAMPKEML PIVDKPTIQY IIEEAVEAGI
60 70 80 90 100
EDIIIVTGKS KRAIEDHFDY SPELERNLEE KGKTELLEKV KKASNLADIH
110 120 130 140 150
YIRQKEPKGL GHAVWCARNF IGDEPFAVLL GDDIVQAETP GLRQLMDEYE
160 170 180 190 200
KTLSSIIGVQ QVPEEETHRY GIIDPLTSEG RRYQVKNFVE KPPKGTAPSN
210 220 230 240 250
LAILGRYVFT PEIFMYLEEQ QVGAGGEIQL TDAIQKLNEI QRVFAYDFEG
260 270 280 290
KRYDVGEKLG FITTTLEFAM QDKELRDQLV PFMEGLLNKE EI
Length:292
Mass (Da):33,070
Last modified:October 1, 1994 - v1
Checksum:iDFA640840826596F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12272 Unassigned DNA. Translation: AAA71967.1.
Z22516 Genomic DNA. Translation: CAA80241.1.
AL009126 Genomic DNA. Translation: CAB15584.1.
PIRiA40650.
RefSeqiNP_391447.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15584; CAB15584; BSU35670.
GeneIDi936797.
KEGGibsu:BSU35670.
PATRICi18979144. VBIBacSub10457_3734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12272 Unassigned DNA. Translation: AAA71967.1 .
Z22516 Genomic DNA. Translation: CAA80241.1 .
AL009126 Genomic DNA. Translation: CAB15584.1 .
PIRi A40650.
RefSeqi NP_391447.1. NC_000964.3.

3D structure databases

ProteinModelPortali Q05852.
SMRi Q05852. Positions 1-285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q05852. 1 interaction.
MINTi MINT-8365569.
STRINGi 224308.BSU35670.

Proteomic databases

PaxDbi Q05852.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15584 ; CAB15584 ; BSU35670 .
GeneIDi 936797.
KEGGi bsu:BSU35670.
PATRICi 18979144. VBIBacSub10457_3734.

Organism-specific databases

GenoListi BSU35670. [Micado ]

Phylogenomic databases

eggNOGi COG1210.
HOGENOMi HOG000283477.
InParanoidi Q05852.
KOi K00963.
OMAi MDEIMTN.
OrthoDBi EOG6Z9B3V.
PhylomeDBi Q05852.

Enzyme and pathway databases

UniPathwayi UPA00894 .
BioCyci BSUB:BSU35670-MONOMER.
MetaCyc:MONOMER-8824.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF00483. NTP_transferase. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01099. galU. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bacillus subtilis gtaB encodes UDP-glucose pyrophosphorylase and is controlled by stationary-phase transcription factor sigma B."
    Varon D., Boylan S.A., Okamoto K., Price C.W.
    J. Bacteriol. 175:3964-3971(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, ROLE IN GLYCOSYLATION OF WTAS.
    Strain: 168.
  2. "Sequencing and analysis of the divergon comprising gtaB, the structural gene of UDP-glucose pyrophosphorylase of Bacillus subtilis 168."
    Soldo B., Lazarevic V., Margot P., Karamata D.
    J. Gen. Microbiol. 139:3185-3195(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
    Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
    Electrophoresis 18:1451-1463(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-21.
    Strain: 168 / IS58.
  5. "The gtaB marker in Bacillus subtilis 168 is associated with a deficiency in UDPglucose pyrophosphorylase."
    Pooley H.M., Paschoud D., Karamata D.
    J. Gen. Microbiol. 133:3481-3493(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 168.
  6. "Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation."
    Lazarevic V., Soldo B., Medico N., Pooley H.M., Bron S., Karamata D.
    Appl. Environ. Microbiol. 71:39-45(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN BIOFILM FORMATION, PATHWAY.
    Strain: 168.

Entry informationi

Entry nameiGTAB_BACSU
AccessioniPrimary (citable) accession number: Q05852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3