Reviewed, UniProtKB/Swiss-Prot Q05852 (GTAB_BACSU)

Names and origin

Protein names

Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase
    EC=2.7.7.9
Alternative name(s):
    UDP-glucose pyrophosphorylase
      Short name=UDPGP
    Alpha-D-glucosyl-1-phosphate uridylyltransferase
    Uridine diphosphoglucose pyrophosphorylase
    General stress protein 33
      Short name=GSP33

Gene names

Name:	gtaB
Ordered Locus Names:	BSU35670

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP. This is an intermediate step in the biosynthesis of diglucosyl-diacylglycerol (Glc2-DAG), i.e. the predominant glycolipid found in B.subtilis membrane, which is also used as a membrane anchor for lipoteichoic acid (LTA). Has a role in the biosynthesis of all phosphate-containing envelope polymers, since UDP-glucose serves as a glucosyl donor not only for the biosynthesis of LTA but also for wall teichoic acids (WTAs). Is required for biofilm formation. This is likely due to another role of UDP-glucose, which might also act as a metabolic signal regulating biofilm formation or may be involved in some unknown biosynthetic pathway essential for biofilm formation, e.g. the synthesis of an exopolysaccharide. Ref.1 Ref.2 Ref.5 Ref.6
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Pathway	Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis. Ref.6
Induction	By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. Expressed under control of the sigma-B transcription factor. Ref.1
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Stress response
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201364

Sequences

	Sequence		Length	Mass (Da)
	Q05852-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: DFA640840826596F	FASTA	292	33,070
	10 20 30 40 50 60 MKKVRKAIIP AAGLGTRFLP ATKAMPKEML PIVDKPTIQY IIEEAVEAGI EDIIIVTGKS 70 80 90 100 110 120 KRAIEDHFDY SPELERNLEE KGKTELLEKV KKASNLADIH YIRQKEPKGL GHAVWCARNF 130 140 150 160 170 180 IGDEPFAVLL GDDIVQAETP GLRQLMDEYE KTLSSIIGVQ QVPEEETHRY GIIDPLTSEG 190 200 210 220 230 240 RRYQVKNFVE KPPKGTAPSN LAILGRYVFT PEIFMYLEEQ QVGAGGEIQL TDAIQKLNEI 250 260 270 280 290 QRVFAYDFEG KRYDVGEKLG FITTTLEFAM QDKELRDQLV PFMEGLLNKE EI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacillus subtilis gtaB encodes UDP-glucose pyrophosphorylase and is controlled by stationary-phase transcription factor sigma B." Varon D., Boylan S.A., Okamoto K., Price C.W. J. Bacteriol. 175:3964-3971(1993) [PubMed: 8320212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, ROLE IN GLYCOSYLATION OF WTAS. Strain: 168.
[2]	"Sequencing and analysis of the divergon comprising gtaB, the structural gene of UDP-glucose pyrophosphorylase of Bacillus subtilis 168." Soldo B., Lazarevic V., Margot P., Karamata D. J. Gen. Microbiol. 139:3185-3195(1993) [PubMed: 8126437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis." Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M. Electrophoresis 18:1451-1463(1997) [PubMed: 9298659] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21. Strain: 168 / IS58.
[5]	"The gtaB marker in Bacillus subtilis 168 is associated with a deficiency in UDPglucose pyrophosphorylase." Pooley H.M., Paschoud D., Karamata D. J. Gen. Microbiol. 133:3481-3493(1987) [PubMed: 2846750] [Abstract] Cited for: FUNCTION. Strain: 168.
[6]	"Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation." Lazarevic V., Soldo B., Medico N., Pooley H.M., Bron S., Karamata D. Appl. Environ. Microbiol. 71:39-45(2005) [PubMed: 15640167] [Abstract] Cited for: ROLE IN BIOFILM FORMATION, PATHWAY. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12272 Unassigned DNA. Translation: AAA71967.1. Z22516 Genomic DNA. Translation: CAA80241.1. AL009126 Genomic DNA. Translation: CAB15584.1.
PIR	A40650.
RefSeq	NP_391447.1.
3D structure databases
SMR	Q05852. Positions 2-285.
ModBase	Search...
Genome annotation databases
GeneID	936797.
GenomeReviews	Gene locus BSU35670 in contig AL009126_GR.
KEGG	bsu:BSU35670.
NMPDR	fig|224308.1.peg.3573.
Organism-specific databases
SubtiList	BG10402. gtaB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG688195.
OMA	HETHAST.
PhylomeDB	Q05852.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-8824.
BRENDA	2.7.7.9. 150.
Family and domain databases
InterPro	IPR005771. GalU_uridylyltTrfase_bac/arc. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01099. galU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GTAB_BACSU

Accession

Primary (citable) accession number: Q05852

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)