Reviewed, UniProtKB/Swiss-Prot Q05820 (LYSC2_RAT)

Names and origin

Protein names

Recommended name:
    Putative lysozyme C-2
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase C

Gene names

Name:

Lyz2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Uncertain.

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. In the intestine they may also have a digestive function.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.
Caution	Could be the product of a pseudogene. Lyz1 has been shown to be expressed, but not Lyz2.

Ontologies

Keywords
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to Gram-negative bacterium Inferred from mutant phenotype. Source: RGD defense response to Gram-positive bacterium Inferred from mutant phenotype. Source: RGD
Cellular component	Golgi cis cisterna Inferred from direct assay. Source: RGD microvillus Inferred from direct assay. Source: RGD rough endoplasmic reticulum lumen Inferred from direct assay. Source: RGD secretory granule Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD trans-Golgi network transport vesicle Inferred from direct assay. Source: RGD
Molecular function	lysozyme activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 148	130	Putative lysozyme C-2		PRO_0000018485
Sites
	Active site	53	1	By similarity
	Active site	71	1	By similarity
Amino acid modifications
	Disulfide bond	24 ↔ 146		By similarity
	Disulfide bond	48 ↔ 134		By similarity
	Disulfide bond	83 ↔ 99		By similarity
	Disulfide bond	95 ↔ 113		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05820-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 2855279E91CCC083	FASTA	148	16,618
	10 20 30 40 50 60 MKALLVLGFL LLSASVQAKV FKHCELARIL RSSALAGYRG VSLENWMCMA QHESNFDTEA 70 80 90 100 110 120 INYNSTDQST DYGIFQINSR YWCNDGKTPR AVNACGIPCS ALLQDDITQA IQCAKRVVRD 130 140 PQGIRAWVAW QRHCQNRDLS GYIRNCGV

References

[1]	"Evolution of rodent lysozymes: isolation and sequence of the rat lysozyme genes." Yeh T.C., Wilson A.C., Irwin D.M. Mol. Phylogenet. Evol. 2:65-75(1993) [PubMed: 8081549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12458 Genomic DNA. Translation: AAA41552.1.
IPI	IPI00193370.
PIR	B40729.
UniGene	Rn.162941
3D structure databases
HSSP	HSSP built from PDB template 1IVM based on UniProtKB P08905.
SMR	Q05820. Positions 19-148.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Proteomic databases
PRIDE	Q05820.
Phylogenomic databases
HOVERGEN	Q05820.
Enzyme and pathway databases
BRENDA	3.2.1.17. 248.
Gene expression databases
Genevestigator	Q05820.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC2_RAT

Accession

Primary (citable) accession number: Q05820

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	October 13, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)