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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Cinara cedri (strain Cc)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation
Metal bindingi927 – 9271ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH372461:GHAJ-93-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BCc_093
OrganismiBuchnera aphidicola subsp. Cinara cedri (strain Cc)
Taxonomic identifieri372461 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000669 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Isoleucine--tRNA ligasePRO_1000022044Add
BLAST

Proteomic databases

PRIDEiQ057X9.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi372461.BCc_093.

Structurei

3D structure databases

ProteinModelPortaliQ057X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q057X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINIKKSLNL PKTKFPMKAN LAYKENEILE TWKKINLYNK LHKNKKKNKQ
60 70 80 90 100
FFLQDGPPYA NGNIHIGHAV NKILKDIILK FKRMSGFFSP YIPCWDCHGL
110 120 130 140 150
PIEHIIEKKL SKKKINKKEF RKICFKYVLK QVEKQKNDFI RLGIIANWDN
160 170 180 190 200
INLSTDYINQ SNTIKVLTKI VEKGLIYRDL KPVYWCFDCQ SALAEAEIEY
210 220 230 240 250
KFKKSISIYI EYKLIENSIL KNNFFKNYNK KIFNNISILI FTTTPWTIPT
260 270 280 290 300
CQAIAINPKL YYQIIKINKK YYICIEELTK KIFKKNNIKK WKIILSFKGK
310 320 330 340 350
EIEHIKCFHP FLNTQIPIIL SKHVSNQLGT GAVHMSPDHG YEDFIACKKY
360 370 380 390 400
KIIPKQIVDS HGFYKIKKYS QLNNIHIFNK ENKIIYILKK NKKLFFFQTI
410 420 430 440 450
NHNYPHCWRH KKPIIFRATP QWFINLSKKN FKEDTFNKIK KILWIPSWGK
460 470 480 490 500
NKMKKMLKIR PDWCISRQRI WGIPLPFFIH KNTGELHPNT VMIMKKIVKK
510 520 530 540 550
IRNHGYKIWW ESNVNTWIKK DSETYRKVND VLDVWFESGA NHQLKIYKHN
560 570 580 590 600
IKNKKNYVAD LYLEGSDQHR GWFMSSLIIS MITKTIPPYL SVITHGFVLD
610 620 630 640 650
KNGQKMSKSL NNNISPKKII QKKGADILRL WVAYTNYTND ISISNEILEQ
660 670 680 690 700
ISDNYRRIRN TIRFLFSNIF DFKANIHIIK YEKMLFLDQW IIEKTYNYQR
710 720 730 740 750
KIIKKYSQYQ FHKVIKKIIN FCSIELGSCY LELIKDRQYT MHKNSIERRS
760 770 780 790 800
SQTAIFYILQ FLVRWIAPIL SFTAEEIWSQ LQEKKEKSIF MTQWYKNKQL
810 820 830 840 850
IKKKSTYNLF FWKKIFAIRK EINLFIETEK KNKFIKNSLE IILLLYINKK
860 870 880 890 900
LFNFLLLFNN ELKFIFLVSE TQLHKYSSAP SLAIKSKKIK KFKILIKKSK
910 920 930 940
KIKCPRCWNY TKKNNFLKNK NSICNKCIKN INQTNKKHIF L
Length:941
Mass (Da):112,919
Last modified:November 14, 2006 - v1
Checksum:iD00F6241B1F556BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000263 Genomic DNA. Translation: ABJ90570.1.
RefSeqiWP_011672489.1. NC_008513.1.
YP_802663.1. NC_008513.1.

Genome annotation databases

EnsemblBacteriaiABJ90570; ABJ90570; BCc_093.
KEGGibcc:BCc_093.
PATRICi21246129. VBIBucAph7855_0089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000263 Genomic DNA. Translation: ABJ90570.1.
RefSeqiWP_011672489.1. NC_008513.1.
YP_802663.1. NC_008513.1.

3D structure databases

ProteinModelPortaliQ057X9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi372461.BCc_093.

Proteomic databases

PRIDEiQ057X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ90570; ABJ90570; BCc_093.
KEGGibcc:BCc_093.
PATRICi21246129. VBIBucAph7855_0089.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBAPH372461:GHAJ-93-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A small microbial genome: the end of a long symbiotic relationship?"
    Perez-Brocal V., Gil R., Ramos S., Lamelas A., Postigo M., Michelena J.M., Silva F.J., Moya A., Latorre A.
    Science 314:312-313(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Cc.

Entry informationi

Entry nameiSYI_BUCCC
AccessioniPrimary (citable) accession number: Q057X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: May 27, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.