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Q057X9

- SYI_BUCCC

UniProt

Q057X9 - SYI_BUCCC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Cinara cedri (strain Cc)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation
    Metal bindingi927 – 9271ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAPH372461:GHAJ-93-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BCc_093
    OrganismiBuchnera aphidicola subsp. Cinara cedri (strain Cc)
    Taxonomic identifieri372461 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000669: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 941941Isoleucine--tRNA ligasePRO_1000022044Add
    BLAST

    Proteomic databases

    PRIDEiQ057X9.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi372461.BCc_093.

    Structurei

    3D structure databases

    ProteinModelPortaliQ057X9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q057X9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINIKKSLNL PKTKFPMKAN LAYKENEILE TWKKINLYNK LHKNKKKNKQ    50
    FFLQDGPPYA NGNIHIGHAV NKILKDIILK FKRMSGFFSP YIPCWDCHGL 100
    PIEHIIEKKL SKKKINKKEF RKICFKYVLK QVEKQKNDFI RLGIIANWDN 150
    INLSTDYINQ SNTIKVLTKI VEKGLIYRDL KPVYWCFDCQ SALAEAEIEY 200
    KFKKSISIYI EYKLIENSIL KNNFFKNYNK KIFNNISILI FTTTPWTIPT 250
    CQAIAINPKL YYQIIKINKK YYICIEELTK KIFKKNNIKK WKIILSFKGK 300
    EIEHIKCFHP FLNTQIPIIL SKHVSNQLGT GAVHMSPDHG YEDFIACKKY 350
    KIIPKQIVDS HGFYKIKKYS QLNNIHIFNK ENKIIYILKK NKKLFFFQTI 400
    NHNYPHCWRH KKPIIFRATP QWFINLSKKN FKEDTFNKIK KILWIPSWGK 450
    NKMKKMLKIR PDWCISRQRI WGIPLPFFIH KNTGELHPNT VMIMKKIVKK 500
    IRNHGYKIWW ESNVNTWIKK DSETYRKVND VLDVWFESGA NHQLKIYKHN 550
    IKNKKNYVAD LYLEGSDQHR GWFMSSLIIS MITKTIPPYL SVITHGFVLD 600
    KNGQKMSKSL NNNISPKKII QKKGADILRL WVAYTNYTND ISISNEILEQ 650
    ISDNYRRIRN TIRFLFSNIF DFKANIHIIK YEKMLFLDQW IIEKTYNYQR 700
    KIIKKYSQYQ FHKVIKKIIN FCSIELGSCY LELIKDRQYT MHKNSIERRS 750
    SQTAIFYILQ FLVRWIAPIL SFTAEEIWSQ LQEKKEKSIF MTQWYKNKQL 800
    IKKKSTYNLF FWKKIFAIRK EINLFIETEK KNKFIKNSLE IILLLYINKK 850
    LFNFLLLFNN ELKFIFLVSE TQLHKYSSAP SLAIKSKKIK KFKILIKKSK 900
    KIKCPRCWNY TKKNNFLKNK NSICNKCIKN INQTNKKHIF L 941
    Length:941
    Mass (Da):112,919
    Last modified:November 14, 2006 - v1
    Checksum:iD00F6241B1F556BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000263 Genomic DNA. Translation: ABJ90570.1.
    RefSeqiYP_802663.1. NC_008513.1.

    Genome annotation databases

    EnsemblBacteriaiABJ90570; ABJ90570; BCc_093.
    GeneIDi4440638.
    KEGGibcc:BCc_093.
    PATRICi21246129. VBIBucAph7855_0089.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000263 Genomic DNA. Translation: ABJ90570.1 .
    RefSeqi YP_802663.1. NC_008513.1.

    3D structure databases

    ProteinModelPortali Q057X9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 372461.BCc_093.

    Proteomic databases

    PRIDEi Q057X9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABJ90570 ; ABJ90570 ; BCc_093 .
    GeneIDi 4440638.
    KEGGi bcc:BCc_093.
    PATRICi 21246129. VBIBucAph7855_0089.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BAPH372461:GHAJ-93-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A small microbial genome: the end of a long symbiotic relationship?"
      Perez-Brocal V., Gil R., Ramos S., Lamelas A., Postigo M., Michelena J.M., Silva F.J., Moya A., Latorre A.
      Science 314:312-313(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Cc.

    Entry informationi

    Entry nameiSYI_BUCCC
    AccessioniPrimary (citable) accession number: Q057X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3