Reviewed, UniProtKB/Swiss-Prot Q057K3 (PNP_BUCCC)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	BCc_227

Organism

Buchnera aphidicola subsp. Cinara cedri [Complete proteome] [HAMAP]

Taxonomic identifier

372461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329550
Regions
	Domain	554 – 613	60	KH
	Domain	623 – 691	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q057K3-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 92B9BFD73C99FFC1	FASTA	693	77,154
	10 20 30 40 50 60 MLKPIIHKFK YGKHNIILET GVIARQATAS VLASMDDTTV LITIVSDNTT IPGQKFFPLV 70 80 90 100 110 120 VNYQERTYAA GRIPGGFFRR EGRPSENEIL ISRLIDRPIR PLFPKDFLYE VQIIATVISV 130 140 150 160 170 180 NPQINPDIIS IIGSSAALCL SGLPFLGPIG AARIGFLNNK YILNPSIEKI KKSSLDLVVS 190 200 210 220 230 240 GTKNTIFMVE AEASILSEDN ILNAILFGHE SQQSLIDNIY LFSDKANKIP NIYKNIYKPD 250 260 270 280 290 300 KQIFNLISKK IKKDINKAYY ILKKKERLKK LNDIKQKVTS ELLNNDSQLT SSKIEESIYF 310 320 330 340 350 360 LERNIVRKRI LKGKLRIDGR INNEVRKIDV RTGILPRVHG SALFTRGETQ ALVSATLGTS 370 380 390 400 410 420 RDAQNLDDLL GDRTDNFLFH YNFPPYSVGE IGIVGSPKRR EIGHGKLAKR SFLAVMPNIE 430 440 450 460 470 480 DFPYTIRLVS EITESNGSSS MASVCGASLA LMDAGVPIKS AIAGIAMGLI KENDSYIILS 490 500 510 520 530 540 DILGDEDYLG DMDFKVSGSR LGITALQMDI KVSGITSDII RSALYQAKSA RLKILDIMEN 550 560 570 580 590 600 TLQIPRSDIS KFAPRIYTIK INPEKIKDVI GKGGSIIRML TEETGTVIEI KDDGIVKISA 610 620 630 640 650 660 INGEKAKYAI KRIQEITEDI QIGKIYSGKV TRILDFGAFV SIGFGKEGLI HISQISNKRI 670 680 690 DKVINHLKIN QIIFVKVLEV DRQGRIRLSM KNI

References

[1]	"A small microbial genome: the end of a long symbiotic relationship?" Perez-Brocal V., Gil R., Ramos S., Lamelas A., Postigo M., Michelena J.M., Silva F.J., Moya A., Latorre A. Science 314:312-313(2006) [PubMed: 17038625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000263 Genomic DNA. Translation: ABJ90696.1.
RefSeq	YP_802789.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4440802.
GenomeReviews	Gene locus BCc_227 in contig CP000263_GR.
KEGG	bcc:BCc_227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q057K3. AFDEIRP.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_BUCCC

Accession

Primary (citable) accession number: Q057K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	November 14, 2006
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)