ID   PNPH_YEAST              Reviewed;         311 AA.
AC   Q05788;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 74.
DE   RecName: Full=Purine nucleoside phosphorylase;
DE            Short=PNP;
DE            EC=2.4.2.1;
DE   AltName: Full=Inosine phosphorylase;
GN   Name=PNP1; OrderedLocusNames=YLR209C; ORFNames=L8167.19;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313267; PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
RA   Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
RA   Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
RA   Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
RA   Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
RA   Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
RA   Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
RA   Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
RA   Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
RA   Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
RA   Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
RA   Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [3]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, AND MASS
RP   SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Cleavage of guanosine or inosine to respective bases and
CC       sugar-1-phosphate molecules (By similarity).
CC   -!- CATALYTIC ACTIVITY: Purine nucleoside + phosphate = purine +
CC       alpha-D-ribose 1-phosphate.
CC   -!- INTERACTION:
CC       Q02821:SRP1; NbExp=1; IntAct=EBI-13604, EBI-1797;
CC   -!- MISCELLANEOUS: Present with 1630 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the PNP/MTAP phosphorylase family.
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DR   EMBL; U14913; AAB67440.1; -; Genomic_DNA.
DR   EMBL; AY557950; AAS56276.1; -; Genomic_DNA.
DR   PIR; S48560; S48560.
DR   RefSeq; NP_013310.1; -.
DR   HSSP; P55859; 1VFN.
DR   DIP; DIP:4300N; -.
DR   IntAct; Q05788; 2.
DR   PRIDE; Q05788; -.
DR   Ensembl; YLR209C; Saccharomyces cerevisiae.
DR   GeneID; 850906; -.
DR   GenomeReviews; Y13138_GR; YLR209C.
DR   KEGG; sce:YLR209C; -.
DR   NMPDR; fig|4932.3.peg.4320; -.
DR   CYGD; YLR209c; -.
DR   SGD; S000004199; PNP1.
DR   HOGENOM; Q05788; -.
DR   OMA; Q05788; DHINFTG.
DR   BRENDA; 2.4.2.1; 250.
DR   NextBio; 967302; -.
DR   ArrayExpress; Q05788; -.
DR   GermOnline; YLR209C; Saccharomyces cerevisiae.
DR   GO; GO:0005622; C:intracellular; IC:SGD.
DR   GO; GO:0047724; F:inosine nucleosidase activity; IDA:SGD.
DR   GO; GO:0070635; F:nicotinamide riboside hydrolase activity; IDA:SGD.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0004731; F:purine-nucleoside phosphorylase activity; IDA:SGD.
DR   GO; GO:0046115; P:guanosine catabolic process; IMP:SGD.
DR   GO; GO:0006148; P:inosine catabolic process; IMP:SGD.
DR   GO; GO:0034356; P:NAD biosynthesis via nicotinamide riboside ...; IGI:SGD.
DR   GO; GO:0019358; P:nicotinate nucleotide salvage; IGI:SGD.
DR   InterPro; IPR000845; Nucleoside_phosphorylase.
DR   InterPro; IPR011268; PNPHPUNA_XAPA.
DR   InterPro; IPR011270; Pur_Nuc_Pase_Ino/Guo-sp.
DR   InterPro; IPR001369; Purine_phosphorylase-2.
DR   InterPro; IPR018099; Purine_phosphorylase-2_CS.
DR   PANTHER; PTHR11904; Mtap_PNP; 1.
DR   PANTHER; PTHR11904:SF9; PNPHPUNA_XAPA; 1.
DR   Pfam; PF01048; PNP_UDP_1; 1.
DR   TIGRFAMs; TIGR01700; PNPH; 1.
DR   TIGRFAMs; TIGR01697; PNPH-PUNA-XAPA; 1.
DR   PROSITE; PS01240; PNP_MTAP_2; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Glycosyltransferase; Phosphoprotein; Transferase.
FT   CHAIN         1    311       Purine nucleoside phosphorylase.
FT                                /FTId=PRO_0000184538.
FT   MOD_RES     275    275       Phosphoserine.
SQ   SEQUENCE   311 AA;  33755 MW;  159DB9F9EC393BCA CRC64;
     MSDILNVSQQ REAITKAAAY ISAILEPHFK NTTNFEPPRT LIICGSGLGG ISTKLSRDNP
     PPVTVPYQDI PGFKKSTVPG HSGTLMFGSM NGSPVVLMNG RLHGYEGNTL FETTFPIRVL
     NHMGHVRNLI VTNAAGGINA KYQACDLMCI YDHLNIPGLA GQHPLRGPNL DEDGPRFLAL
     SDAYDLELRK LLFKKWKELK IQRPLHEGTY TFVSGPTFET RAESKMIRML GGDAVGMSTV
     PEVIVARHCG WRVLALSLIT NTCVVDSPAS ALDESPVPLE KGKATHAEVL ENGKIASNDV
     QNLIAAVMGE L
//