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Q05788

- PNPH_YEAST

UniProt

Q05788 - PNPH_YEAST

Protein

Purine nucleoside phosphorylase

Gene

PNP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine and inosine By similarity.By similarity

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. inosine nucleosidase activity Source: SGD
    2. nicotinamide riboside hydrolase activity Source: SGD
    3. purine-nucleoside phosphorylase activity Source: SGD

    GO - Biological processi

    1. guanosine catabolic process Source: SGD
    2. inosine catabolic process Source: SGD
    3. NAD biosynthesis via nicotinamide riboside salvage pathway Source: SGD
    4. nicotinate nucleotide salvage Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YLR209C-MONOMER.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purine nucleoside phosphorylase (EC:2.4.2.1)
    Short name:
    PNP
    Alternative name(s):
    Inosine phosphorylase
    Inosine-guanosine phosphorylase
    Gene namesi
    Name:PNP1
    Ordered Locus Names:YLR209C
    ORF Names:L8167.19
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR209c.
    SGDiS000004199. PNP1.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 311310Purine nucleoside phosphorylasePRO_0000184538Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei275 – 2751Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ05788.
    PaxDbiQ05788.

    Expressioni

    Gene expression databases

    GenevestigatoriQ05788.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SRP1Q028211EBI-13604,EBI-1797

    Protein-protein interaction databases

    BioGridi31477. 7 interactions.
    DIPiDIP-4300N.
    IntActiQ05788. 3 interactions.
    MINTiMINT-550315.
    STRINGi4932.YLR209C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05788.
    SMRiQ05788. Positions 12-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0005.
    GeneTreeiENSGT00550000074740.
    HOGENOMiHOG000045183.
    KOiK03783.
    OMAiDHINMLP.
    OrthoDBiEOG72C59D.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    InterProiIPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR011268. Purine_phosphorylase.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PTHR11904:SF9. PTHR11904:SF9. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000477. PurNPase. 1 hit.
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01697. PNPH-PUNA-XAPA. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05788-1 [UniParc]FASTAAdd to Basket

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    MSDILNVSQQ REAITKAAAY ISAILEPHFK NTTNFEPPRT LIICGSGLGG    50
    ISTKLSRDNP PPVTVPYQDI PGFKKSTVPG HSGTLMFGSM NGSPVVLMNG 100
    RLHGYEGNTL FETTFPIRVL NHMGHVRNLI VTNAAGGINA KYQACDLMCI 150
    YDHLNIPGLA GQHPLRGPNL DEDGPRFLAL SDAYDLELRK LLFKKWKELK 200
    IQRPLHEGTY TFVSGPTFET RAESKMIRML GGDAVGMSTV PEVIVARHCG 250
    WRVLALSLIT NTCVVDSPAS ALDESPVPLE KGKATHAEVL ENGKIASNDV 300
    QNLIAAVMGE L 311
    Length:311
    Mass (Da):33,755
    Last modified:November 1, 1996 - v1
    Checksum:i159DB9F9EC393BCA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14913 Genomic DNA. Translation: AAB67440.1.
    AY557950 Genomic DNA. Translation: AAS56276.1.
    BK006945 Genomic DNA. Translation: DAA09526.1.
    PIRiS48560.
    RefSeqiNP_013310.1. NM_001182096.1.

    Genome annotation databases

    EnsemblFungiiYLR209C; YLR209C; YLR209C.
    GeneIDi850906.
    KEGGisce:YLR209C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14913 Genomic DNA. Translation: AAB67440.1 .
    AY557950 Genomic DNA. Translation: AAS56276.1 .
    BK006945 Genomic DNA. Translation: DAA09526.1 .
    PIRi S48560.
    RefSeqi NP_013310.1. NM_001182096.1.

    3D structure databases

    ProteinModelPortali Q05788.
    SMRi Q05788. Positions 12-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31477. 7 interactions.
    DIPi DIP-4300N.
    IntActi Q05788. 3 interactions.
    MINTi MINT-550315.
    STRINGi 4932.YLR209C.

    Proteomic databases

    MaxQBi Q05788.
    PaxDbi Q05788.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR209C ; YLR209C ; YLR209C .
    GeneIDi 850906.
    KEGGi sce:YLR209C.

    Organism-specific databases

    CYGDi YLR209c.
    SGDi S000004199. PNP1.

    Phylogenomic databases

    eggNOGi COG0005.
    GeneTreei ENSGT00550000074740.
    HOGENOMi HOG000045183.
    KOi K03783.
    OMAi DHINMLP.
    OrthoDBi EOG72C59D.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .
    BioCyci YEAST:YLR209C-MONOMER.

    Miscellaneous databases

    NextBioi 967302.
    PROi Q05788.

    Gene expression databases

    Genevestigatori Q05788.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    InterProi IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR011268. Purine_phosphorylase.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    PTHR11904:SF9. PTHR11904:SF9. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000477. PurNPase. 1 hit.
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01697. PNPH-PUNA-XAPA. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPNPH_YEAST
    AccessioniPrimary (citable) accession number: Q05788
    Secondary accession number(s): D6VYL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1630 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3