Q05788 (PNPH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purine nucleoside phosphorylase
Short name=PNP
EC=2.4.2.1

Alternative name(s):
Inosine phosphorylase

Gene names

Name:	PNP1
Ordered Locus Names:	YLR209C
ORF Names:	L8167.19

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules By similarity.
Catalytic activity	Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the PNP/MTAP phosphorylase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	guanosine catabolic process Inferred from mutant phenotype. Source: SGD inosine catabolic process Inferred from mutant phenotype. Source: SGD nicotinate nucleotide salvage Inferred from genetic interaction. Source: SGD
Cellular component	intracellular Inferred by curator. Source: SGD
Molecular function	inosine nucleosidase activity Inferred from direct assay. Source: SGD nicotinamide riboside hydrolase activity Inferred from direct assay. Source: SGD purine-nucleoside phosphorylase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRP1	Q02821	1	EBI-13604,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Purine nucleoside phosphorylase		PRO_0000184538
Amino acid modifications
	Modified residue	275	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q05788 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 159DB9F9EC393BCA	FASTA	311	33,755
	10 20 30 40 50 60 MSDILNVSQQ REAITKAAAY ISAILEPHFK NTTNFEPPRT LIICGSGLGG ISTKLSRDNP 70 80 90 100 110 120 PPVTVPYQDI PGFKKSTVPG HSGTLMFGSM NGSPVVLMNG RLHGYEGNTL FETTFPIRVL 130 140 150 160 170 180 NHMGHVRNLI VTNAAGGINA KYQACDLMCI YDHLNIPGLA GQHPLRGPNL DEDGPRFLAL 190 200 210 220 230 240 SDAYDLELRK LLFKKWKELK IQRPLHEGTY TFVSGPTFET RAESKMIRML GGDAVGMSTV 250 260 270 280 290 300 PEVIVARHCG WRVLALSLIT NTCVVDSPAS ALDESPVPLE KGKATHAEVL ENGKIASNDV 310 QNLIAAVMGE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14913 Genomic DNA. Translation: AAB67440.1. AY557950 Genomic DNA. Translation: AAS56276.1. BK006945 Genomic DNA. Translation: DAA09526.1.
PIR	S48560.
RefSeq	NP_013310.1. NM_001182096.1.
3D structure databases
ProteinModelPortal	Q05788.
SMR	Q05788. Positions 12-311.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4300N.
IntAct	Q05788. 1 interaction.
MINT	MINT-550315.
STRING	Q05788.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR209C; YLR209C; YLR209C.
GeneID	850906.
KEGG	sce:YLR209C.
NMPDR	fig|4932.3.peg.4320.
Organism-specific databases
CYGD	YLR209c.
SGD	S000004199. PNP1.
Phylogenomic databases
eggNOG	fuNOG04872.
GeneTree	EFGT00050000004672.
HOGENOM	HBG292914.
OMA	MKVMTTP.
OrthoDB	EOG46MFTQ.
Gene expression databases
ArrayExpress	Q05788.
Genevestigator	Q05788.
GermOnline	YLR209C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011268. InoGua/Xao_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. IPR001369. Purine_phosphorylase-2. IPR018099. Purine_phosphorylase-2_CS. [Graphical view]
KO	K03783.
PANTHER	PTHR11904. Mtap_PNP. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
PIRSF	PIRSF000477. PurNPase. 1 hit.
TIGRFAMs	TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITE	PS01240. PNP_MTAP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967302.

Entry information

Entry name

PNPH_YEAST

Accession

Primary (citable) accession number: Q05788
Secondary accession number(s): D6VYL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program