Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ERAD-associated E3 ubiquitin-protein ligase component HRD3

Gene

HRD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the endoplasmic reticulum quality control (ERQC) system involved in ubiquitin-dependent degradation of missfolded endoplasmic reticulum proteins. Component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome. ERAD-M substrates are processed by the same HRD1-HRD3 core complex, but only a subset of the other components is required for ERAD-M. Stabilizes the HRD1 ubiquitin-protein ligase. Has also a function in recruiting misfolded protein substrates.10 Publications

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein ubiquitination Source: GOC
  • retrograde protein transport, ER to cytosol Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32325-MONOMER.

Protein family/group databases

TCDBi3.A.16.1.2. the endoplasmic reticular retrotranslocon (er-rt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
ERAD-associated E3 ubiquitin-protein ligase component HRD3
Alternative name(s):
HMG-CoA reductase degradation protein 3
Gene namesi
Name:HRD3
Ordered Locus Names:YLR207W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR207W.
SGDiS000004197. HRD3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei768 – 788HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
  • Hrd1p ubiquitin ligase ERAD-M complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • luminal surveillance complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000024036921 – 833ERAD-associated E3 ubiquitin-protein ligase component HRD3Add BLAST813

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi101N-linked (GlcNAc...)Sequence analysis1
Glycosylationi123N-linked (GlcNAc...)Sequence analysis1
Glycosylationi142N-linked (GlcNAc...)Sequence analysis1
Glycosylationi429N-linked (GlcNAc...)Sequence analysis1
Glycosylationi611N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ05787.
PRIDEiQ05787.

Interactioni

Subunit structurei

Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, KAR2, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factors KAR2 and YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. The complex interacts with the ERAD substrates HMG1 and HMG2. Interacts with HRD1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC48P256946EBI-31647,EBI-4308
DER1P383073EBI-31647,EBI-5761
HRD1Q081099EBI-31647,EBI-37613
PRC1P007296EBI-31647,EBI-4153
SEC61P329152EBI-31647,EBI-16400
YOS9Q992208EBI-31647,EBI-34938

Protein-protein interaction databases

BioGridi31475. 71 interactors.
DIPiDIP-2843N.
IntActiQ05787. 13 interactors.
MINTiMINT-1722064.

Structurei

3D structure databases

ProteinModelPortaliQ05787.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 139Sel1-like 1Add BLAST37
Repeati143 – 186Sel1-like 2Add BLAST44
Repeati187 – 222Sel1-like 3Add BLAST36
Repeati413 – 445Sel1-like 4Add BLAST33
Repeati552 – 595Sel1-like 5Add BLAST44
Repeati596 – 627Sel1-like 6Add BLAST32
Repeati628 – 663Sel1-like 7Add BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi809 – 819Poly-GlnAdd BLAST11

Sequence similaritiesi

Belongs to the sel-1 family.Curated
Contains 7 Sel1-like repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063170.
HOGENOMiHOG000112949.
InParanoidiQ05787.
KOiK14023.
OMAiSEWIANF.
OrthoDBiEOG092C27J5.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF08238. Sel1. 4 hits.
[Graphical view]
SMARTiSM00671. SEL1. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05787-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITLLLYLCV ICNAIVLIRA DSIADPWPEA RHLLNTIAKS RDPMKEAAME
60 70 80 90 100
PNADEFVGFY VPMDYSPRNE EKNYQSIWQN EITDSQRHIY ELLVQSSEQF
110 120 130 140 150
NNSEATYTLS QIHLWSQYNF PHNMTLAHKY LEKFNDLTHF TNHSAIFDLA
160 170 180 190 200
VMYATGGCAS GNDQTVIPQD SAKALLYYQR AAQLGNLKAK QVLAYKYYSG
210 220 230 240 250
FNVPRNFHKS LVLYRDIAEQ LRKSYSRDEW DIVFPYWESY NVRISDFESG
260 270 280 290 300
LLGKGLNSVP SSTVRKRTTR PDIGSPFIAQ VNGVQMTLQI EPMGRFAFNG
310 320 330 340 350
NDGNINGDED DEDASERRII RIYYAALNDY KGTYSQSRNC ERAKNLLELT
360 370 380 390 400
YKEFQPHVDN LDPLQVFYYV RCLQLLGHMY FTGEGSSKPN IHMAEEILTT
410 420 430 440 450
SLEISRRAQG PIGRACIDLG LINQYITNNI SQAISYYMKA MKTQANNGIV
460 470 480 490 500
EFQLSKLATS FPEEKIGDPF NLMETAYLNG FIPAIYEFAV MIESGMNSKS
510 520 530 540 550
SVENTAYLFK TFVDKNEAIM APKLRTAFAA LINDRSEVAL WAYSQLAEQG
560 570 580 590 600
YETAQVSAAY LMYQLPYEFE DPPRTTDQRK TLAISYYTRA FKQGNIDAGV
610 620 630 640 650
VAGDIYFQMQ NYSKAMALYQ GAALKYSIQA IWNLGYMHEH GLGVNRDFHL
660 670 680 690 700
AKRYYDQVSE HDHRFYLASK LSVLKLHLKS WLTWITREKV NYWKPSSPLN
710 720 730 740 750
PNEDTQHSKT SWYKQLTKIL QRMRHKEDSD KAAEDSHKHR TVVQNGANHR
760 770 780 790 800
GDDQEEASEI LGFQMEDLVT MGCILGIFLL SILMSTLAAR RGWNVRFNGA
810 820 830
QLNANGNRQQ EQQQQQQAQG PPGWDFNVQI FAI
Length:833
Mass (Da):95,481
Last modified:November 1, 1996 - v1
Checksum:iE7DB29FBA16D9BFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14913 Genomic DNA. Translation: AAB67427.1.
BK006945 Genomic DNA. Translation: DAA09524.1.
PIRiS48558.
RefSeqiNP_013308.1. NM_001182094.1.

Genome annotation databases

EnsemblFungiiYLR207W; YLR207W; YLR207W.
GeneIDi850904.
KEGGisce:YLR207W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14913 Genomic DNA. Translation: AAB67427.1.
BK006945 Genomic DNA. Translation: DAA09524.1.
PIRiS48558.
RefSeqiNP_013308.1. NM_001182094.1.

3D structure databases

ProteinModelPortaliQ05787.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31475. 71 interactors.
DIPiDIP-2843N.
IntActiQ05787. 13 interactors.
MINTiMINT-1722064.

Protein family/group databases

TCDBi3.A.16.1.2. the endoplasmic reticular retrotranslocon (er-rt) family.

Proteomic databases

MaxQBiQ05787.
PRIDEiQ05787.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR207W; YLR207W; YLR207W.
GeneIDi850904.
KEGGisce:YLR207W.

Organism-specific databases

EuPathDBiFungiDB:YLR207W.
SGDiS000004197. HRD3.

Phylogenomic databases

GeneTreeiENSGT00530000063170.
HOGENOMiHOG000112949.
InParanoidiQ05787.
KOiK14023.
OMAiSEWIANF.
OrthoDBiEOG092C27J5.

Enzyme and pathway databases

BioCyciYEAST:G3O-32325-MONOMER.

Miscellaneous databases

PROiQ05787.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF08238. Sel1. 4 hits.
[Graphical view]
SMARTiSM00671. SEL1. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHRD3_YEAST
AccessioniPrimary (citable) accession number: Q05787
Secondary accession number(s): D6VYK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.