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Protein

Epsin-2

Gene

ENT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin.1 Publication

Miscellaneous

Present with 1970 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • clathrin binding Source: SGD
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • actin filament organization Source: SGD
  • endocytosis Source: SGD

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32324-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-2
Gene namesi
Name:ENT2
Ordered Locus Names:YLR206W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR206W.
SGDiS000004196. ENT2.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745251 – 613Epsin-2Add BLAST613

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei165PhosphothreonineCombined sources1
Modified residuei167PhosphoserineCombined sources1
Cross-linki426Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei430PhosphothreonineCombined sources1
Modified residuei434PhosphoserineCombined sources1
Modified residuei450PhosphothreonineCombined sources1
Modified residuei468PhosphothreonineCombined sources1
Modified residuei470PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated by PRK1.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ05785.
PRIDEiQ05785.

PTM databases

iPTMnetiQ05785.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • clathrin binding Source: SGD

Protein-protein interaction databases

BioGridi31474. 179 interactors.
DIPiDIP-2698N.
ELMiQ05785.
IntActiQ05785. 20 interactors.
MINTiMINT-687167.
STRINGi4932.YLR206W.

Structurei

Secondary structure

1613
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 26Combined sources9
Beta strandi29 – 31Combined sources3
Helixi36 – 45Combined sources10
Helixi49 – 63Combined sources15
Helixi67 – 69Combined sources3
Helixi70 – 86Combined sources17
Helixi89 – 97Combined sources9
Helixi99 – 104Combined sources6
Helixi105 – 107Combined sources3
Helixi120 – 133Combined sources14
Helixi136 – 145Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GZCX-ray1.30A1-149[»]
4GZDX-ray1.75A1-149[»]
ProteinModelPortaliQ05785.
SMRiQ05785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 143ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini175 – 194UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini206 – 225UIM 2PROSITE-ProRule annotationAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi138 – 166Arg-richAdd BLAST29
Compositional biasi192 – 606Gln-richAdd BLAST415

Sequence similaritiesi

Belongs to the epsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00890000140675.
HOGENOMiHOG000216614.
InParanoidiQ05785.
KOiK12471.
OMAiMVPPQQS.
OrthoDBiEOG092C4BNK.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiView protein in InterPro
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR003903. UIM_dom.
PfamiView protein in Pfam
PF01417. ENTH. 1 hit.
SMARTiView protein in SMART
SM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiView protein in PROSITE
PS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.

Sequencei

Sequence statusi: Complete.

Q05785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQFVRSAK NMMKGYSSTQ VLVRDATAND SRTPSIDTLD DLAQRSYDSV
60 70 80 90 100
DFFEIMDMLD KRLNDKGKYW RHVAKSLTVL DYLVRFGSEN CVLWCRENFY
110 120 130 140 150
VIKTLREFRH ENESGFDEGQ IIRVKAKELV SLLNDEERLR EERSMNTRNR
160 170 180 190 200
RANRAARPRP RRQRTRSNPH DSSPSYQDDL EKALEESRIT AQEDEQRRRE
210 220 230 240 250
LAQYDDEDPD FQAALQLSKE EEELKQLQEL QRLQKQQQSL SQFQAPLQQQ
260 270 280 290 300
QPQQQPAYYD IFGNPISQDE YLQYQYQQDQ EQAMAQQRWL DQQQEQQQLA
310 320 330 340 350
EQQYFQQQQQ AAAAASALQQ QQTAANMQQQ QQQPADFQQP LPTGSNNPFS
360 370 380 390 400
MDNLERQKQE QQHAQLQRQQ EEARQQQEQL KLQQLQRQQQ EEAQLHQKRQ
410 420 430 440 450
EEAQLQQQQA QLLQQQAQFQ QQQPLKQTRT GNQSISDKYS DLNTLLATGT
460 470 480 490 500
GIDTFGNTGE ARIPAQHTKT GTFINSQGTG YKQVTNEPKN NPFLSNQYTG
510 520 530 540 550
LPSTNIVPTQ TGYGFGNQPQ SPPTNSPQQN PTGISYSQPQ QQQQPQQQPQ
560 570 580 590 600
YMQNFQQQQP QYAQNFQQQP QYTQNYQQQP QYIQPHQQQQ QQQQQQQQQQ
610
GYTPDQGVSL IDL
Length:613
Mass (Da):71,807
Last modified:November 1, 1996 - v1
Checksum:iCEC1B2D04C0F7D86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14913 Genomic DNA. Translation: AAB67428.1.
BK006945 Genomic DNA. Translation: DAA09523.1.
PIRiS48557.
RefSeqiNP_013307.1. NM_001182093.1.

Genome annotation databases

EnsemblFungiiYLR206W; YLR206W; YLR206W.
GeneIDi850903.
KEGGisce:YLR206W.

Similar proteinsi

Entry informationi

Entry nameiENT2_YEAST
AccessioniPrimary (citable) accession number: Q05785
Secondary accession number(s): D6VYK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names