Epsin-2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

Q05785 (ENT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Epsin-2

Gene names

Name:	ENT2
Ordered Locus Names:	YLR206W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Ref.3
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: Localizes in a punctate pattern. Found in the actin cortical patches, although the majority is located at the cell periphery. Ref.3 Ref.4
Post-translational modification	Phosphorylated by PRK1. Ref.4
Miscellaneous	Present with 1970 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the epsin family. Contains 1 ENTH (epsin N-terminal homology) domain. Contains 2 UIM (ubiquitin-interacting motif) repeats.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Membrane
Domain	Repeat
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cortical patch assembly Inferred from mutant phenotype Ref.3. Source: SGD actin filament organization Inferred from mutant phenotype Ref.3. Source: SGD endocytosis Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	actin cortical patch Traceable author statement PubMed 10652251. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	clathrin binding Traceable author statement PubMed 12461563. Source: SGD lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 613

613

Epsin-2

PRO_0000074525

Regions

Domain

11 – 143

133

ENTH

Repeat

175 – 194

UIM 1

Repeat

206 – 225

UIM 2

Compositional bias

138 – 166

Arg-rich

Compositional bias

192 – 606

415

Gln-rich

Amino acid modifications

Modified residue

165

Phosphothreonine Ref.7 Ref.9 Ref.10

Modified residue

167

Phosphoserine Ref.7 Ref.9 Ref.10

Modified residue

172

Phosphoserine Ref.10

Modified residue

173

Phosphoserine Ref.9 Ref.10

Modified residue

430

Phosphothreonine Ref.8 Ref.10

Modified residue

434

Phosphoserine Ref.8

Modified residue

436

Phosphoserine Ref.9

Modified residue

450

Phosphothreonine Ref.10

Modified residue

468

Phosphothreonine Ref.7 Ref.9 Ref.10

Modified residue

470

Phosphothreonine Ref.9 Ref.10

Modified residue

476

Phosphoserine Ref.9

Modified residue

479

Phosphothreonine Ref.6 Ref.10

Modified residue

481

Phosphotyrosine Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

Q05785 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CEC1B2D04C0F7D86
Show »

FASTA

613

71,807

        10         20         30         40         50         60 
MSKQFVRSAK NMMKGYSSTQ VLVRDATAND SRTPSIDTLD DLAQRSYDSV DFFEIMDMLD 

        70         80         90        100        110        120 
KRLNDKGKYW RHVAKSLTVL DYLVRFGSEN CVLWCRENFY VIKTLREFRH ENESGFDEGQ 

       130        140        150        160        170        180 
IIRVKAKELV SLLNDEERLR EERSMNTRNR RANRAARPRP RRQRTRSNPH DSSPSYQDDL 

       190        200        210        220        230        240 
EKALEESRIT AQEDEQRRRE LAQYDDEDPD FQAALQLSKE EEELKQLQEL QRLQKQQQSL 

       250        260        270        280        290        300 
SQFQAPLQQQ QPQQQPAYYD IFGNPISQDE YLQYQYQQDQ EQAMAQQRWL DQQQEQQQLA 

       310        320        330        340        350        360 
EQQYFQQQQQ AAAAASALQQ QQTAANMQQQ QQQPADFQQP LPTGSNNPFS MDNLERQKQE 

       370        380        390        400        410        420 
QQHAQLQRQQ EEARQQQEQL KLQQLQRQQQ EEAQLHQKRQ EEAQLQQQQA QLLQQQAQFQ 

       430        440        450        460        470        480 
QQQPLKQTRT GNQSISDKYS DLNTLLATGT GIDTFGNTGE ARIPAQHTKT GTFINSQGTG 

       490        500        510        520        530        540 
YKQVTNEPKN NPFLSNQYTG LPSTNIVPTQ TGYGFGNQPQ SPPTNSPQQN PTGISYSQPQ 

       550        560        570        580        590        600 
QQQQPQQQPQ YMQNFQQQQP QYAQNFQQQP QYTQNYQQQP QYIQPHQQQQ QQQQQQQQQQ 

       610 
GYTPDQGVSL IDL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis." Wendland B., Steece K.E., Emr S.D. EMBO J. 18:4383-4393(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation." Watson H.A., Cope M.J.T.V., Groen A.C., Drubin D.G., Wendland B. Mol. Biol. Cell 12:3668-3679(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-479, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-165; SER-167 AND THR-468, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430 AND SER-434, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-165; SER-167; SER-173; SER-436; THR-468; THR-470 AND SER-476, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-165; SER-167; SER-172; SER-173; THR-430; THR-450; THR-468; THR-470; THR-479 AND TYR-481, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14913 Genomic DNA. Translation: AAB67428.1. BK006945 Genomic DNA. Translation: DAA09523.1.
PIR	S48557.
RefSeq	NP_013307.1. NM_001182093.1.
3D structure databases
ProteinModelPortal	Q05785.
SMR	Q05785. Positions 7-143.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2698N.
IntAct	Q05785. 12 interactions.
MINT	MINT-687167.
STRING	4932.YLR206W.
Proteomic databases
PaxDb	Q05785.
PeptideAtlas	Q05785.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR206W; YLR206W; YLR206W.
GeneID	850903.
KEGG	sce:YLR206W.
Organism-specific databases
CYGD	YLR206w.
SGD	S000004196. ENT2.
Phylogenomic databases
eggNOG	NOG263730.
GeneTree	ENSGT00700000105179.
HOGENOM	HOG000216614.
KO	K12471.
OMA	FVQPQAT.
OrthoDB	EOG4CC79N.
Gene expression databases
Genevestigator	Q05785.
GermOnline	YLR206W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.40.90. 1 hit.
InterPro	IPR008942. ENTH_VHS. IPR013809. Epsin-like_N. IPR001026. Epsin_dom_N. IPR003903. Ubiquitin-int_motif. [Graphical view]
Pfam	PF01417. ENTH. 1 hit. [Graphical view]
SMART	SM00273. ENTH. 1 hit. SM00726. UIM. 2 hits. [Graphical view]
SUPFAM	SSF48464. ENTH_VHS. 1 hit.
PROSITE	PS50942. ENTH. 1 hit. PS50330. UIM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	967293.

Entry information

Entry name

ENT2_YEAST

Accession

Primary (citable) accession number: Q05785
Secondary accession number(s): D6VYK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents