Q05776 (UPS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein UPS1, mitochondrial

Alternative name(s):
Unprocessed MGM1 protein 1

Gene names

Name:	UPS1
Ordered Locus Names:	YLR193C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for maintenance of normal mitochondrial morphology as well as PCP1-dependent processing of MGM1. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits. Ref.5 Ref.6 Ref.7 Ref.8
Subunit structure	Interacts with MDM35. Found associated with a 170 kDa complex. Ref.5 Ref.8 Ref.9
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Mitochondrion intermembrane space. Note: Lacks the two major intermembrane space-targeting signals, bipartite presequences and cysteine motifs, and import is mediated by another IMS protein, MDM35. Ref.3 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Disruption phenotype	Cells show slow growth, fragmented mitochondria and have a 50-fold reduced level of s-MGM1. These defects can be complemented by human PRELI or bypassed by growth on a nonfermentable carbon source. Ref.5
Miscellaneous	Present with 704 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the slowmo family. Contains 1 PRELI/MSF1 domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cardiolipin metabolic process Inferred from mutant phenotype Ref.6Ref.7. Source: SGD
Cellular component	extrinsic to mitochondrial inner membrane Inferred from direct assay Ref.5Ref.7. Source: SGD mitochondrial intermembrane space Inferred from direct assay Ref.5Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Protein UPS1, mitochondrial		PRO_0000271269
Regions
	Domain	2 – 172	171	PRELI/MSF1
	Region	1 – 80	80	Required for mitochondrial targeting Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q05776 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5B9003B6A2323109	FASTA	175	20,108
	10 20 30 40 50 60 MVLLHKSTHI FPTDFASVSR AFFNRYPNPY SPHVLSIDTI SRNVDQEGNL RTTRLLKKSG 70 80 90 100 110 120 KLPTWVKPFL RGITETWIIE VSVVNPANST MKTYTRNLDH TGIMKVEEYT TYQFDSATSS 130 140 150 160 170 TIADSRVKFS SGFNMGIKSK VEDWSRTKFD ENVKKSRMGM AFVIQKLEEA RNPQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Ups1p, a conserved intermembrane space protein, regulates mitochondrial shape and alternative topogenesis of Mgm1p." Sesaki H., Dunn C.D., Iijima M., Shepard K.A., Yaffe M.P., Machamer C.E., Jensen R.E. J. Cell Biol. 173:651-658(2006) [PubMed: 16754953] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]	"The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria." Osman C., Haag M., Potting C., Rodenfels J., Dip P.V., Wieland F.T., Brugger B., Westermann B., Langer T. J. Cell Biol. 184:583-596(2009) [PubMed: 19221197] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria." Tamura Y., Endo T., Iijima M., Sesaki H. J. Cell Biol. 185:1029-1045(2009) [PubMed: 19506038] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Mdm35p imports Ups proteins into the mitochondrial intermembrane space by functional complex formation." Tamura Y., Iijima M., Sesaki H. EMBO J. 29:2875-2887(2010) [PubMed: 20622808] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION MDM35.
[9]	"Regulation of mitochondrial phospholipids by Ups1/PRELI-like proteins depends on proteolysis and Mdm35." Potting C., Wilmes C., Engmann T., Osman C., Langer T. EMBO J. 29:2888-2898(2010) [PubMed: 20657548] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MDM35.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14913 Genomic DNA. Translation: AAB67434.1. BK006945 Genomic DNA. Translation: DAA09512.1.
PIR	S48546.
RefSeq	NP_013294.1. NM_001182080.1.
3D structure databases
ProteinModelPortal	Q05776.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4798N.
MINT	MINT-494469.
STRING	Q05776.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR193C; YLR193C; YLR193C.
GeneID	850890.
KEGG	sce:YLR193C.
NMPDR	fig|4932.3.peg.4304.
Organism-specific databases
CYGD	YLR193c.
SGD	S000004183. UPS1.
Phylogenomic databases
eggNOG	fuNOG10656.
GeneTree	EFGT00050000003515.
HOGENOM	HBG398237.
OMA	RISESWV.
OrthoDB	EOG47WRZ5.
Gene expression databases
ArrayExpress	Q05776.
Genevestigator	Q05776.
Family and domain databases
InterPro	IPR006797. PRELI/MSF1. [Graphical view]
Pfam	PF04707. PRELI. 1 hit. [Graphical view]
PROSITE	PS50904. PRELI_MSF1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967260.

Entry information

Entry name

UPS1_YEAST

Accession

Primary (citable) accession number: Q05776
Secondary accession number(s): D6VYJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program