Reviewed, UniProtKB/Swiss-Prot Q05775 (EIF3J_YEAST)

Names and origin

Protein names

Recommended name:
    Eukaryotic translation initiation factor 3 subunit J
      Short name=eIF3j
Alternative name(s):
    Eukaryotic translation initiation factor 3 30 kDa subunit
      Short name=eIF-3 30 kDa

Gene names

Name:	HCR1
Ordered Locus Names:	YLR192C
ORF Names:	L8167.11

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.
Subunit structure	Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Is not part of the eIF-3 core complex, with which it is associated in substochiometric amounts.
Subcellular location	Cytoplasm Probable.
Disruption phenotype	Marked reduction in binding of the eIF-3 core complex to 40S ribosomes. Ref.5
Miscellaneous	Present with 17900 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the eIF-3 subunit J family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD translation Inferred from physical interaction. Source: SGD
Cellular component	eukaryotic translation initiation factor 3 complex Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct rRNA binding Inferred from direct assay. Source: SGD translation initiation factor activity Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-8944,EBI-22766
ESS1	P22696	1	EBI-8944,EBI-6679
PRP40	P33203	1	EBI-8944,EBI-701
RLI1	Q03195	1	EBI-8944,EBI-35146
RSP5	P39940	1	EBI-8944,EBI-16219
SET2	P46995	1	EBI-8944,EBI-16985
URN1	Q06525	1	EBI-8944,EBI-35138

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Eukaryotic translation initiation factor 3 subunit J		PRO_0000123509
Amino acid modifications
	Modified residue	64	1	Phosphoserine Ref.8
	Modified residue	65	1	Phosphoserine Ref.7
	Modified residue	75	1	Phosphothreonine Ref.8 Ref.6
	Modified residue	189	1	Phosphoserine Ref.8
Experimental info
	Mutagenesis	215	1	R → I: Increased association of the eIF-3 complex and 40S ribosomes. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q05775-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BDDBC5943DD7F9BB	FASTA	265	29,564
	10 20 30 40 50 60 MSWDDEAING SMGNDDAVLM DSWDAEIGDD EPVMQSWDAE EEEKKPAPKP KKEQPKKVKK 70 80 90 100 110 120 GKESSADRAL LDIDTLDEKT RKELIKKAEM ESDLNNAADL FAGLGVAEEH PRARALQKEQ 130 140 150 160 170 180 EEQALKRPAF TKDTPIETHP LFNAETKREY QDLRKALTAA ITPMNKKSPL NYSSSLAIDL 190 200 210 220 230 240 IRDVAKPMSI ESIRQTVATL NVLIKDKERE ERQARLARVR GGTATGGAGK KKVKGKTNLG 250 260 GAFKKDQDFD LDGPDDFEFG DDDFM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3." Valasek L., Hasek J., Trachsel H., Imre E.M., Ruis H. J. Biol. Chem. 274:27567-27572(1999) [PubMed: 10488093] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast." Nielsen K.H., Valasek L., Sykes C., Jivotovskaya A., Hinnebusch A.G. Mol. Cell. Biol. 26:2984-2998(2006) [PubMed: 16581774] [Abstract] Cited for: INTERACTION WITH PRT1, ASSOCIATION WITH THE 40S RIBOSOME, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-215.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; THR-75 AND SER-189, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U14913 Genomic DNA. Translation: AAB67433.1. AY557943 Genomic DNA. Translation: AAS56269.1.
PIR	S48545.
RefSeq	NP_013293.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4532N.
IntAct	Q05775. 22 interactions.
Proteomic databases
PeptideAtlas	Q05775.
Genome annotation databases
Ensembl	YLR192C. Saccharomyces cerevisiae. [Contig view]
GeneID	850889.
GenomeReviews	Gene locus YLR192C in contig Y13138_GR.
KEGG	sce:YLR192C.
NMPDR	fig|4932.3.peg.4303.
Organism-specific databases
CYGD	YLR192c.
SGD	S000004182. HCR1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q05775.
OMA	Q05775. MSWDDED.
Gene expression databases
GermOnline	YLR192C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013906. eIF3_subunit. [Graphical view]
PANTHER	PTHR21681. eIF3_subunit. 1 hit.
Pfam	PF08597. eIF3_subunit. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967257.

Entry information

Entry name

EIF3J_YEAST

Accession

Primary (citable) accession number: Q05775

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)