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Reviewed, UniProtKB/Swiss-Prot Q05775 (EIF3J_YEAST)

Last modified June 16, 2009. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Eukaryotic translation initiation factor 3 subunit J
      Short name=eIF3j
Alternative name(s):
    Eukaryotic translation initiation factor 3 30 kDa subunit
      Short name=eIF-3 30 kDa
Gene names
Name: HCR1
Ordered Locus Names: YLR192C
ORF Names: L8167.11
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.

Subunit structure

Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Is not part of the eIF-3 core complex, with which it is associated in substochiometric amounts.

Subcellular location

Cytoplasm Probable.

Disruption phenotype

Marked reduction in binding of the eIF-3 core complex to 40S ribosomes. Ref.5

Miscellaneous

Present with 17900 molecules/cell in log phase SD medium. Ref.4

Sequence similarities

Belongs to the eIF-3 subunit J family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Eukaryotic translation initiation factor 3 subunit J
PRO_0000123509

Amino acid modifications

Modified residue641Phosphoserine Ref.8
Modified residue651Phosphoserine Ref.7
Modified residue751Phosphothreonine Ref.8 Ref.6
Modified residue1891Phosphoserine Ref.8

Experimental info

Mutagenesis2151R → I: Increased association of the eIF-3 complex and 40S ribosomes. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q05775-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BDDBC5943DD7F9BB

FASTA26529,564
        10         20         30         40         50         60 
MSWDDEAING SMGNDDAVLM DSWDAEIGDD EPVMQSWDAE EEEKKPAPKP KKEQPKKVKK 

        70         80         90        100        110        120 
GKESSADRAL LDIDTLDEKT RKELIKKAEM ESDLNNAADL FAGLGVAEEH PRARALQKEQ 

       130        140        150        160        170        180 
EEQALKRPAF TKDTPIETHP LFNAETKREY QDLRKALTAA ITPMNKKSPL NYSSSLAIDL 

       190        200        210        220        230        240 
IRDVAKPMSI ESIRQTVATL NVLIKDKERE ERQARLARVR GGTATGGAGK KKVKGKTNLG 

       250        260 
GAFKKDQDFD LDGPDDFEFG DDDFM 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed: 9169871] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3."
Valasek L., Hasek J., Trachsel H., Imre E.M., Ruis H.
J. Biol. Chem. 274:27567-27572(1999) [PubMed: 10488093] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast."
Nielsen K.H., Valasek L., Sykes C., Jivotovskaya A., Hinnebusch A.G.
Mol. Cell. Biol. 26:2984-2998(2006) [PubMed: 16581774] [Abstract]
Cited for: INTERACTION WITH PRT1, ASSOCIATION WITH THE 40S RIBOSOME, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-215.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, MASS SPECTROMETRY.
[7]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; THR-75 AND SER-189, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

U14913 Genomic DNA. Translation: AAB67433.1.
AY557943 Genomic DNA. Translation: AAS56269.1.
PIRS48545.
RefSeqNP_013293.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4532N.
IntActQ05775. 22 interactions.

Proteomic databases

PeptideAtlasQ05775.

Genome annotation databases

EnsemblYLR192C. Saccharomyces cerevisiae. [Contig view]
GeneID850889.
GenomeReviewsGene locus YLR192C in contig Y13138_GR.
KEGGsce:YLR192C.
NMPDRfig|4932.3.peg.4303.

Organism-specific databases

CYGDYLR192c.
SGDS000004182. HCR1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMQ05775.
OMAQ05775. MSWDDED.

Gene expression databases

GermOnlineYLR192C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013906. eIF3_subunit.
[Graphical view]
PANTHERPTHR21681. eIF3_subunit. 1 hit.
PfamPF08597. eIF3_subunit. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio967257.

Entry information

Entry nameEIF3J_YEAST
AccessionPrimary (citable) accession number: Q05775
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Translation initiation factors

List of translation initiation factor entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents