Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q05775

- EIF3J_YEAST

UniProt

Q05775 - EIF3J_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Eukaryotic translation initiation factor 3 subunit J

Gene

HCR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.

GO - Molecular functioni

  1. translation initiation factor activity Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. formation of translation preinitiation complex Source: UniProtKB-HAMAP
  3. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. regulation of translational initiation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-32314-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit JUniRule annotation
Short name:
eIF3jUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 30 kDa subunit
Short name:
eIF-3 30 kDa
Gene namesi
Name:HCR1UniRule annotation
Ordered Locus Names:YLR192C
ORF Names:L8167.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR192c.
SGDiS000004182. HCR1.

Subcellular locationi

Cytoplasm 1 PublicationUniRule annotation

GO - Cellular componenti

  1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
  2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
  3. eukaryotic translation initiation factor 3 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Marked reduction in binding of the eIF-3 core complex to 40S ribosomes.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi215 – 2151R → I: Increased association of the eIF-3 complex and 40S ribosomes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Eukaryotic translation initiation factor 3 subunit JPRO_0000123509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei75 – 751Phosphothreonine3 PublicationsUniRule annotation
Modified residuei92 – 921Phosphoserine1 PublicationUniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05775.
PaxDbiQ05775.
PeptideAtlasiQ05775.

Expressioni

Gene expression databases

GenevestigatoriQ05775.

Interactioni

Subunit structurei

Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Is not part of the eIF-3 core complex, with which it is associated in substochiometric amounts.

Binary interactionsi

WithEntry#Exp.IntActNotes
RLI1Q031954EBI-8944,EBI-35146

Protein-protein interaction databases

BioGridi31462. 134 interactions.
DIPiDIP-4532N.
IntActiQ05775. 23 interactions.
MINTiMINT-517104.
STRINGi4932.YLR192C.

Structurei

3D structure databases

ProteinModelPortaliQ05775.
SMRiQ05775. Positions 138-213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-3 subunit J family.UniRule annotation

Phylogenomic databases

eggNOGiNOG118058.
HOGENOMiHOG000246847.
InParanoidiQ05775.
KOiK03245.
OMAiLESWDID.
OrthoDBiEOG76X6BX.

Family and domain databases

Gene3Di1.10.246.60. 1 hit.
HAMAPiMF_03009. eIF3j.
InterProiIPR023194. eIF3-like_dom.
IPR013906. eIF3j.
[Graphical view]
PANTHERiPTHR21681. PTHR21681. 1 hit.
PfamiPF08597. eIF3_subunit. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05775-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSWDDEAING SMGNDDAVLM DSWDAEIGDD EPVMQSWDAE EEEKKPAPKP
60 70 80 90 100
KKEQPKKVKK GKESSADRAL LDIDTLDEKT RKELIKKAEM ESDLNNAADL
110 120 130 140 150
FAGLGVAEEH PRARALQKEQ EEQALKRPAF TKDTPIETHP LFNAETKREY
160 170 180 190 200
QDLRKALTAA ITPMNKKSPL NYSSSLAIDL IRDVAKPMSI ESIRQTVATL
210 220 230 240 250
NVLIKDKERE ERQARLARVR GGTATGGAGK KKVKGKTNLG GAFKKDQDFD
260
LDGPDDFEFG DDDFM
Length:265
Mass (Da):29,564
Last modified:November 1, 1996 - v1
Checksum:iBDDBC5943DD7F9BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14913 Genomic DNA. Translation: AAB67433.1.
AY557943 Genomic DNA. Translation: AAS56269.1.
BK006945 Genomic DNA. Translation: DAA09511.1.
PIRiS48545.
RefSeqiNP_013293.1. NM_001182079.1.

Genome annotation databases

EnsemblFungiiYLR192C; YLR192C; YLR192C.
GeneIDi850889.
KEGGisce:YLR192C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14913 Genomic DNA. Translation: AAB67433.1 .
AY557943 Genomic DNA. Translation: AAS56269.1 .
BK006945 Genomic DNA. Translation: DAA09511.1 .
PIRi S48545.
RefSeqi NP_013293.1. NM_001182079.1.

3D structure databases

ProteinModelPortali Q05775.
SMRi Q05775. Positions 138-213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31462. 134 interactions.
DIPi DIP-4532N.
IntActi Q05775. 23 interactions.
MINTi MINT-517104.
STRINGi 4932.YLR192C.

Proteomic databases

MaxQBi Q05775.
PaxDbi Q05775.
PeptideAtlasi Q05775.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR192C ; YLR192C ; YLR192C .
GeneIDi 850889.
KEGGi sce:YLR192C.

Organism-specific databases

CYGDi YLR192c.
SGDi S000004182. HCR1.

Phylogenomic databases

eggNOGi NOG118058.
HOGENOMi HOG000246847.
InParanoidi Q05775.
KOi K03245.
OMAi LESWDID.
OrthoDBi EOG76X6BX.

Enzyme and pathway databases

BioCyci YEAST:G3O-32314-MONOMER.

Miscellaneous databases

NextBioi 967257.
PROi Q05775.

Gene expression databases

Genevestigatori Q05775.

Family and domain databases

Gene3Di 1.10.246.60. 1 hit.
HAMAPi MF_03009. eIF3j.
InterProi IPR023194. eIF3-like_dom.
IPR013906. eIF3j.
[Graphical view ]
PANTHERi PTHR21681. PTHR21681. 1 hit.
Pfami PF08597. eIF3_subunit. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3."
    Valasek L., Hasek J., Trachsel H., Imre E.M., Ruis H.
    J. Biol. Chem. 274:27567-27572(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast."
    Nielsen K.H., Valasek L., Sykes C., Jivotovskaya A., Hinnebusch A.G.
    Mol. Cell. Biol. 26:2984-2998(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRT1, ASSOCIATION WITH THE 40S RIBOSOME, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-215.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75 AND SER-92, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEIF3J_YEAST
AccessioniPrimary (citable) accession number: Q05775
Secondary accession number(s): D6VYJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3