Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q05775

- EIF3J_YEAST

UniProt

Q05775 - EIF3J_YEAST

Protein

Eukaryotic translation initiation factor 3 subunit J

Gene

HCR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. translation initiation factor activity Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. formation of translation preinitiation complex Source: UniProtKB-HAMAP
    3. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. regulation of translational initiation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32314-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 3 subunit JUniRule annotation
    Short name:
    eIF3jUniRule annotation
    Alternative name(s):
    Eukaryotic translation initiation factor 3 30 kDa subunit
    Short name:
    eIF-3 30 kDa
    Gene namesi
    Name:HCR1UniRule annotation
    Ordered Locus Names:YLR192C
    ORF Names:L8167.11
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR192c.
    SGDiS000004182. HCR1.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
    2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
    3. eukaryotic translation initiation factor 3 complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Marked reduction in binding of the eIF-3 core complex to 40S ribosomes.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi215 – 2151R → I: Increased association of the eIF-3 complex and 40S ribosomes. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 265265Eukaryotic translation initiation factor 3 subunit JPRO_0000123509Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei75 – 751Phosphothreonine3 PublicationsUniRule annotation
    Modified residuei92 – 921Phosphoserine1 PublicationUniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ05775.
    PaxDbiQ05775.
    PeptideAtlasiQ05775.

    Expressioni

    Gene expression databases

    GenevestigatoriQ05775.

    Interactioni

    Subunit structurei

    Probable component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Is not part of the eIF-3 core complex, with which it is associated in substochiometric amounts.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RLI1Q031954EBI-8944,EBI-35146

    Protein-protein interaction databases

    BioGridi31462. 134 interactions.
    DIPiDIP-4532N.
    IntActiQ05775. 23 interactions.
    MINTiMINT-517104.
    STRINGi4932.YLR192C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05775.
    SMRiQ05775. Positions 138-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eIF-3 subunit J family.UniRule annotation

    Phylogenomic databases

    eggNOGiNOG118058.
    HOGENOMiHOG000246847.
    KOiK03245.
    OMAiLESWDID.
    OrthoDBiEOG76X6BX.

    Family and domain databases

    Gene3Di1.10.246.60. 1 hit.
    HAMAPiMF_03009. eIF3j.
    InterProiIPR023194. eIF3-like_dom.
    IPR013906. eIF3j.
    [Graphical view]
    PANTHERiPTHR21681. PTHR21681. 1 hit.
    PfamiPF08597. eIF3_subunit. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05775-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSWDDEAING SMGNDDAVLM DSWDAEIGDD EPVMQSWDAE EEEKKPAPKP    50
    KKEQPKKVKK GKESSADRAL LDIDTLDEKT RKELIKKAEM ESDLNNAADL 100
    FAGLGVAEEH PRARALQKEQ EEQALKRPAF TKDTPIETHP LFNAETKREY 150
    QDLRKALTAA ITPMNKKSPL NYSSSLAIDL IRDVAKPMSI ESIRQTVATL 200
    NVLIKDKERE ERQARLARVR GGTATGGAGK KKVKGKTNLG GAFKKDQDFD 250
    LDGPDDFEFG DDDFM 265
    Length:265
    Mass (Da):29,564
    Last modified:November 1, 1996 - v1
    Checksum:iBDDBC5943DD7F9BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14913 Genomic DNA. Translation: AAB67433.1.
    AY557943 Genomic DNA. Translation: AAS56269.1.
    BK006945 Genomic DNA. Translation: DAA09511.1.
    PIRiS48545.
    RefSeqiNP_013293.1. NM_001182079.1.

    Genome annotation databases

    EnsemblFungiiYLR192C; YLR192C; YLR192C.
    GeneIDi850889.
    KEGGisce:YLR192C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14913 Genomic DNA. Translation: AAB67433.1 .
    AY557943 Genomic DNA. Translation: AAS56269.1 .
    BK006945 Genomic DNA. Translation: DAA09511.1 .
    PIRi S48545.
    RefSeqi NP_013293.1. NM_001182079.1.

    3D structure databases

    ProteinModelPortali Q05775.
    SMRi Q05775. Positions 138-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31462. 134 interactions.
    DIPi DIP-4532N.
    IntActi Q05775. 23 interactions.
    MINTi MINT-517104.
    STRINGi 4932.YLR192C.

    Proteomic databases

    MaxQBi Q05775.
    PaxDbi Q05775.
    PeptideAtlasi Q05775.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR192C ; YLR192C ; YLR192C .
    GeneIDi 850889.
    KEGGi sce:YLR192C.

    Organism-specific databases

    CYGDi YLR192c.
    SGDi S000004182. HCR1.

    Phylogenomic databases

    eggNOGi NOG118058.
    HOGENOMi HOG000246847.
    KOi K03245.
    OMAi LESWDID.
    OrthoDBi EOG76X6BX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32314-MONOMER.

    Miscellaneous databases

    NextBioi 967257.
    PROi Q05775.

    Gene expression databases

    Genevestigatori Q05775.

    Family and domain databases

    Gene3Di 1.10.246.60. 1 hit.
    HAMAPi MF_03009. eIF3j.
    InterProi IPR023194. eIF3-like_dom.
    IPR013906. eIF3j.
    [Graphical view ]
    PANTHERi PTHR21681. PTHR21681. 1 hit.
    Pfami PF08597. eIF3_subunit. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3."
      Valasek L., Hasek J., Trachsel H., Imre E.M., Ruis H.
      J. Biol. Chem. 274:27567-27572(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast."
      Nielsen K.H., Valasek L., Sykes C., Jivotovskaya A., Hinnebusch A.G.
      Mol. Cell. Biol. 26:2984-2998(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRT1, ASSOCIATION WITH THE 40S RIBOSOME, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-215.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75 AND SER-92, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEIF3J_YEAST
    AccessioniPrimary (citable) accession number: Q05775
    Secondary accession number(s): D6VYJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 17900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3