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Protein

Beta-adducin

Gene

Add2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit (By similarity).By similarity

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: RGD

GO - Biological processi

  • ion transport Source: RGD
  • protein complex assembly Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-RNO-5223345. Miscellaneous transport and binding events.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-adducin
Alternative name(s):
Adducin-63
Erythrocyte adducin subunit beta
Gene namesi
Name:Add2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2042. Add2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB
  • cytoskeleton Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002185352 – 725Beta-adducinAdd BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei11PhosphoserineCombined sources1
Modified residuei25PhosphoserineCombined sources1
Modified residuei55PhosphothreonineBy similarity1
Modified residuei60PhosphoserineCombined sources1
Modified residuei344PhosphoserineBy similarity1
Modified residuei396PhosphoserineBy similarity1
Modified residuei455PhosphoserineBy similarity1
Modified residuei530PhosphoserineCombined sources1
Modified residuei532PhosphoserineCombined sources1
Modified residuei533PhosphothreonineBy similarity1
Modified residuei535PhosphoserineBy similarity1
Modified residuei594PhosphoserineCombined sources1
Modified residuei598PhosphoserineBy similarity1
Modified residuei602PhosphoserineCombined sources1
Modified residuei606PhosphoserineBy similarity1
Modified residuei612PhosphothreonineBy similarity1
Modified residuei614PhosphoserineCombined sources1
Modified residuei618PhosphoserineCombined sources1
Modified residuei620PhosphoserineBy similarity1
Modified residuei674PhosphothreonineCombined sources1
Modified residuei678PhosphoserineCombined sources1
Modified residuei685PhosphoserineCombined sources1
Modified residuei688PhosphoserineCombined sources1
Modified residuei692PhosphoserineCombined sources1
Modified residuei696PhosphoserineBy similarity1
Modified residuei698PhosphoserineBy similarity1
Modified residuei700PhosphoserineBy similarity1
Modified residuei702PhosphoserineBy similarity1
Modified residuei712PhosphoserineBy similarity1
Isoform 2 (identifier: Q05764-2)
Modified residuei561PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ05764.
PRIDEiQ05764.

PTM databases

iPTMnetiQ05764.
PhosphoSitePlusiQ05764.

Expressioni

Tissue specificityi

Found in liver, kidney, spleen, heart and brain.

Gene expression databases

ExpressionAtlasiQ05764. baseline and differential.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Found in a complex with ADD2, DMTN and SLC2A1. Interacts with SLC2A1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021491.

Structurei

3D structure databases

ProteinModelPortaliQ05764.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni425 – 444Interaction with calmodulinSequence analysisAdd BLAST20
Regioni703 – 720Interaction with calmodulinSequence analysisAdd BLAST18

Domaini

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ05764.
KOiK18622.
PhylomeDBiQ05764.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027773. ADD2.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF6. PTHR10672:SF6. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q05764-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEDTVPEAA SPPPSQGQHY FDRFSEDDPE YLRLRNRAAD LRQDFNLMEQ
60 70 80 90 100
KKRVTMILQS PSFREELEGL IQEQMKKGNN SSNIWALRQI ADFMASTSHA
110 120 130 140 150
VFPASSMNFS MMTPINDLHT ADSLNLAKGE RLMRCKISSV YRLLDLYGWA
160 170 180 190 200
QLSDTYVTLR VSKEQDHFLI SPKGVSCSEV TASSLIKVNI LGEVVEKGSS
210 220 230 240 250
CFPVDTTGFS LHSAIYAARP DVRCAIHLHT PATAAVSAMK CGLLPVSHNA
260 270 280 290 300
LLVGDMAYYD FNGEMEQEAD RINLQKCLGP TCKILVLRNH GMVALGDTVE
310 320 330 340 350
EAFYKVFHLQ AACEVQVSAL SSAGGTENLI LLEQEKHRPH EVGSVQWAGS
360 370 380 390 400
TFGPMQKSRL GEHEFEALMR MLDNLGYRTG YTYRHPFVQE KTKHKSEVEI
410 420 430 440 450
PATVTAFVFE EDGVPVPALR QHAQKQQKEK TRWLNTPNTY LRVNVADEVQ
460 470 480 490 500
RNMGSPRPKT TWMKADEVEK SSSGMPIRIE NPNQFVPLYT DPQEVLDMRN
510 520 530 540 550
KIREQNRQDI KSAGPQSQLL ASVIAEKSRS PSTESQLASK GDADTKDELE
560 570 580 590 600
ETVPNPFSQL TDQELEEYKK EVERKKLEQE QEGEKDAATE EPGSPVKSTP
610 620 630 640 650
ASPVQSPTRA GTKSPAVSPS KASEDAKKTE VSEANTEPEP EKPEGVVVNG
660 670 680 690 700
KEEEPCVEEV LSKGPGQMTT NADTDGDSYK DKTESVTSGP LSPEGSPSKS
710 720
PSKKKKKFRT PSFLKKSKKK EKVES
Length:725
Mass (Da):80,593
Last modified:January 23, 2007 - v4
Checksum:i0715BD6A75B3D174
GO
Isoform 2 (identifier: Q05764-2) [UniParc]FASTAAdd to basket
Also known as: Adducin 63

The sequence of this isoform differs from the canonical sequence as follows:
     532-562: STESQLASKGDADTKDELEETVPNPFSQLTD → VQQRLPPTEGEAYQTPGAGQGTPESSGPLTP
     563-725: Missing.

Show »
Length:562
Mass (Da):62,788
Checksum:iAECF299694E44CB8
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti529R → Q in strain: Milan hypertensive. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000186532 – 562STESQ…SQLTD → VQQRLPPTEGEAYQTPGAGQ GTPESSGPLTP in isoform 2. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_000187563 – 725Missing in isoform 2. 1 PublicationAdd BLAST163

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63894 mRNA. Translation: AAA40679.1.
AF130338 mRNA. Translation: AAF31764.1.
PIRiJQ1036.
RefSeqiNP_001103350.1. NM_001109880.1.
NP_036623.1. NM_012491.2. [Q05764-2]
UniGeneiRn.11353.

Genome annotation databases

EnsembliENSRNOT00000081173; ENSRNOP00000073973; ENSRNOG00000015903. [Q05764-2]
GeneIDi24171.
KEGGirno:24171.
UCSCiRGD:2042. rat. [Q05764-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63894 mRNA. Translation: AAA40679.1.
AF130338 mRNA. Translation: AAF31764.1.
PIRiJQ1036.
RefSeqiNP_001103350.1. NM_001109880.1.
NP_036623.1. NM_012491.2. [Q05764-2]
UniGeneiRn.11353.

3D structure databases

ProteinModelPortaliQ05764.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021491.

PTM databases

iPTMnetiQ05764.
PhosphoSitePlusiQ05764.

Proteomic databases

PaxDbiQ05764.
PRIDEiQ05764.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000081173; ENSRNOP00000073973; ENSRNOG00000015903. [Q05764-2]
GeneIDi24171.
KEGGirno:24171.
UCSCiRGD:2042. rat. [Q05764-1]

Organism-specific databases

CTDi119.
RGDi2042. Add2.

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ05764.
KOiK18622.
PhylomeDBiQ05764.

Enzyme and pathway databases

ReactomeiR-RNO-5223345. Miscellaneous transport and binding events.

Miscellaneous databases

PROiQ05764.

Gene expression databases

ExpressionAtlasiQ05764. baseline and differential.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027773. ADD2.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF6. PTHR10672:SF6. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADDB_RAT
AccessioniPrimary (citable) accession number: Q05764
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.