Reviewed,
UniProtKB/Swiss-Prot Q05762 (DRTS1_ARATH)
Last modified
February 9, 2010.
Version 85.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Bifunctional dihydrofolate reductase-thymidylate synthase 1 Short name=DHFR-TS 1 Including the following 2 domains: 1- Recommended name: Dihydrofolate reductase EC=1.5.1.3 2- Recommended name: Thymidylate synthase EC=2.1.1.45 | ||||||
| Gene names |
| ||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 519 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP. |
| Catalytic activity | 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. |
| Pathway | |
| Subunit structure | Heterodimer or homodimer By similarity. |
| Sequence similarities | In the N-terminal section; belongs to the dihydrofolate reductase family. In the C-terminal section; belongs to the thymidylate synthase family. Contains 1 DHFR (dihydrofolate reductase) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Nucleotide biosynthesis One-carbon metabolism |
| Ligand | NADP |
| Molecular function | Methyltransferase Oxidoreductase Transferase |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro glycine biosynthetic processInferred from electronic annotation. Source: InterPro one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | dihydrofolate reductase activity Inferred from electronic annotation. Source: EC thymidylate synthase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 519 | 519 | Bifunctional dihydrofolate reductase-thymidylate synthase 1 | PRO_0000186355 | |||||
Regions | |||||||||
| Domain | 21 – 198 | 178 | DHFR | ||||||
| Nucleotide binding | 33 – 39 | 7 | NADP By similarity | ||||||
| Nucleotide binding | 71 – 73 | 3 | NADP By similarity | ||||||
| Nucleotide binding | 92 – 95 | 4 | NADP By similarity | ||||||
| Nucleotide binding | 135 – 142 | 8 | NADP By similarity | ||||||
| Nucleotide binding | 420 – 424 | 5 | dUMP By similarity | ||||||
| Nucleotide binding | 462 – 464 | 3 | dUMP By similarity | ||||||
| Region | 201 – 234 | 34 | Hinge | ||||||
| Region | 235 – 519 | 285 | Thymidylate synthase | ||||||
Sites | |||||||||
| Active site | 401 | 1 | By similarity | ||||||
| Binding site | 25 | 1 | Substrate; via carbonyl oxygen By similarity | ||||||
| Binding site | 27 | 1 | NADP; via amide nitrogen and carbonyl oxygen By similarity | ||||||
| Binding site | 47 | 1 | Substrate By similarity | ||||||
| Binding site | 134 | 1 | Substrate; via carbonyl oxygen By similarity | ||||||
| Binding site | 155 | 1 | Substrate By similarity | ||||||
| Binding site | 256 | 1 | dUMP By similarity | ||||||
| Binding site | 402 | 1 | dUMP By similarity | ||||||
| Binding site | 432 | 1 | dUMP By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 120 | 1 | A → R in AAA32788. Ref.1 | ||||||
| Sequence conflict | 478 | 1 | L → P in AAA32788. Ref.1 | ||||||
| Sequence conflict | 485 | 1 | M → L in AAA32788. Ref.1 | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L08593 mRNA. Translation: AAA32788.1. AC007047 Genomic DNA. Translation: AAD22302.1. AY063968 mRNA. Translation: AAL36324.1. AY114032 mRNA. Translation: AAM45080.1. |
| IPI | IPI00530946. |
| PIR | E84539. |
| RefSeq | NP_179230.1. |
| UniGene | At.27124 |
3D structure databases | |
| SMR | Q05762. Positions 17-513, 234-519. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q05762. |
Proteomic databases | |
| PRIDE | Q05762. |
Genome annotation databases | |
| GeneID | 816134. |
| GenomeReviews | Gene locus AT2G16370 in contig CT485783_GR. |
| KEGG | ath:AT2G16370. |
| NMPDR | fig|3702.1.peg.8626. |
Organism-specific databases | |
| TAIR | At2g16370. |
Phylogenomic databases | |
| eggNOG | KOG0673. |
| HOGENOM | HBG588098. |
| InParanoid | Q05762. |
| OMA | HADYTGK. |
| PhylomeDB | Q05762. |
Enzyme and pathway databases | |
| BRENDA | 1.5.1.3. 302. 2.1.1.45. 302. |
Gene expression databases | |
| ArrayExpress | Q05762. |
| Genevestigator | Q05762. |
| GermOnline | AT2G16370. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR012262. DHFR-TS. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view] |
| Gene3D | G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit. |
| PANTHER | PTHR11549:SF2. Thymidylat_synth_C. 1 hit. |
| Pfam | PF00186. DHFR_1. 1 hit. PF00303. Thymidylat_synt. 1 hit. [Graphical view] |
| PIRSF | PIRSF000389. DHFR-TS. 1 hit. |
| PRINTS | PR00108. THYMDSNTHASE. |
| TIGRFAMs | TIGR03284. thym_sym. 1 hit. |
| PROSITE | PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DRTS1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q05762 Secondary accession number(s): Q9SIW4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


