Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ketol-acid reductoisomerase, chloroplastic

Gene

At3g58610

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase (AXX17_At3g42690), Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase small subunit 2, chloroplastic (At2g31810)
  2. Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610), Ketol-acid reductoisomerase (AXX17_At3g53150)
  3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
  4. Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase (AXX17_At3g42690), Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase small subunit 2, chloroplastic (At2g31810)
  2. Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610), Ketol-acid reductoisomerase (AXX17_At3g53150)
  3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
  4. Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei220Sequence analysis1
Metal bindingi309Magnesium 1PROSITE-ProRule annotation1
Metal bindingi309Magnesium 2PROSITE-ProRule annotation1
Metal bindingi313Magnesium 1PROSITE-ProRule annotation1
Metal bindingi486Magnesium 2PROSITE-ProRule annotation1
Metal bindingi490Magnesium 2PROSITE-ProRule annotation1
Binding sitei512SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 130NADPBy similarity8
Nucleotide bindingi156 – 161NADPBy similarity6
Nucleotide bindingi195 – 199NADPBy similarity5

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • ketol-acid reductoisomerase activity Source: UniProtKB-EC

GO - Biological processi

  • isoleucine biosynthetic process Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
  • valine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT3G58610-MONOMER
UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase, chloroplastic (EC:1.1.1.86)
Alternative name(s):
Acetohydroxy-acid reductoisomerase
Alpha-keto-beta-hydroxylacyl reductoisomerase
Gene namesi
Ordered Locus Names:At3g58610
ORF Names:F14P22.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G58610
TAIRilocus:2076371 AT3G58610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastCombined sourcesAdd BLAST52
ChainiPRO_000001562953 – 591Ketol-acid reductoisomerase, chloroplasticAdd BLAST539

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ05758
PRIDEiQ05758

PTM databases

iPTMnetiQ05758

Expressioni

Gene expression databases

ExpressionAtlasiQ05758 baseline and differential
GenevisibleiQ05758 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi10345, 2 interactors
STRINGi3702.AT3G58610.1

Structurei

3D structure databases

ProteinModelPortaliQ05758
SMRiQ05758
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 300KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST199
Domaini301 – 449KARI C-terminal knotted 1PROSITE-ProRule annotationAdd BLAST149
Domaini450 – 586KARI C-terminal knotted 2PROSITE-ProRule annotationAdd BLAST137

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IEEV Eukaryota
COG0059 LUCA
HOGENOMiHOG000238129
InParanoidiQ05758
KOiK00053
OMAiEEIFSHM
OrthoDBiEOG093606Q2
PhylomeDBiQ05758

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR000506 AcH_isomrdctse_C
IPR013116 IlvN
IPR013023 KARI
IPR016206 KetolA_reductoisomerase_plant
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 2 hits
PF07991 IlvN, 1 hit
PIRSFiPIRSF000118 Ilv5_plant, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 2 hits
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATSSIAP SLSCPSPSSS SKTLWSSKAR TLALPNIGFL SSSSKSLRSL
60 70 80 90 100
TATVAGNGAT GSSLAARMVS SSAVKAPVSL DFETSVFKKE KVSLAGYEEY
110 120 130 140 150
IVRGGRDLFK HLPDAFKGIK QIGVIGWGSQ GPAQAQNLRD SLVEAKSDIV
160 170 180 190 200
VKIGLRKGSR SFEEARAAGF TEESGTLGDI WETIAGSDLV LLLISDAAQA
210 220 230 240 250
DNYEKIFSHM KPNSILGLSH GFLLGHLQSS GLDFPKNISV VAVCPKGMGP
260 270 280 290 300
SVRRLYVQGK EINGAGINAS FAVHQDVDGR AADVALGWSV ALGSPFTFAT
310 320 330 340 350
TLEQEYRSDI FGERGILLGA VHGIVESLFR RYTENGMSED LAYKNTVECI
360 370 380 390 400
TGTISRTIST QGMLAVYNSL SEEGKKDFET AYSASFYPCM EILYECYEDV
410 420 430 440 450
QSGSEIRSVV LAGRRFYEKE GLPAFPMGNI DQTRMWKVGE RVRKSRPAGD
460 470 480 490 500
LGPLYPFTAG VYVALMMAQI EILRKKGHSY SEIINESVIE SVDSLNPFMH
510 520 530 540 550
ARGVSFMVDN CSTTARLGSR KWAPRFDYIL TQQALVAVDS GAAINRDLIS
560 570 580 590
NFFSDPVHGA IEVCAQLRPT VDISVPADAD FVRPELRQSS N
Length:591
Mass (Da):63,812
Last modified:December 1, 2000 - v2
Checksum:i003C41A69B2C0F4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti285A → R in CAA48253 (PubMed:8448371).Curated1
Sequence conflicti579A → V in CAA48253 (PubMed:8448371).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68150 mRNA Translation: CAA48253.1
X69880 Genomic DNA Translation: CAA49506.1
AL137082 Genomic DNA Translation: CAB68199.1
CP002686 Genomic DNA Translation: AEE79804.1
CP002686 Genomic DNA Translation: AEE79805.1
CP002686 Genomic DNA Translation: AEE79806.1
AF324671 mRNA Translation: AAG40022.1
AF329500 mRNA Translation: AAG42917.1
AY062094 mRNA Translation: AAL32973.1
AY065398 mRNA Translation: AAL38839.1
AY096556 mRNA Translation: AAM20206.1
BT000669 mRNA Translation: AAN31816.1
BT000822 mRNA Translation: AAN33197.1
PIRiS30145
T45681
RefSeqiNP_001078309.1, NM_001084840.1
NP_001190127.1, NM_001203198.1
NP_191420.1, NM_115723.4
UniGeneiAt.46637
At.480
At.69012

Genome annotation databases

EnsemblPlantsiAT3G58610.1; AT3G58610.1; AT3G58610
AT3G58610.2; AT3G58610.2; AT3G58610
AT3G58610.3; AT3G58610.3; AT3G58610
GeneIDi825030
GrameneiAT3G58610.1; AT3G58610.1; AT3G58610
AT3G58610.2; AT3G58610.2; AT3G58610
AT3G58610.3; AT3G58610.3; AT3G58610
KEGGiath:AT3G58610

Similar proteinsi

Entry informationi

Entry nameiILV5_ARATH
AccessioniPrimary (citable) accession number: Q05758
Secondary accession number(s): Q42559
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 1, 2000
Last modified: April 25, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health