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Q05753

- AKRP_ARATH

UniProt

Q05753 - AKRP_ARATH

Protein

Ankyrin repeat domain-containing protein, chloroplastic

Gene

AKRP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (13 Dec 2002)
      Previous versions | rss
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    Functioni

    Involved in the initial differentiation of the proplastid during the embryo development and in plastid differentiation linked to cell differentiation, morphogenesis and organogenesis during the plant life cycle.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Enzyme and pathway databases

    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ankyrin repeat domain-containing protein, chloroplastic
    Short name:
    AKRP
    Alternative name(s):
    Protein EMBRYO DEFECTIVE 2036
    Gene namesi
    Name:AKRP
    Synonyms:AKR, EMB2036
    Ordered Locus Names:At5g66055
    ORF Names:K2A18.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G66055.

    Subcellular locationi

    Plastidchloroplast 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Plants show a developmental arrest of the embryos at the globular stage.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3636ChloroplastSequence AnalysisAdd
    BLAST
    Chaini37 – 435399Ankyrin repeat domain-containing protein, chloroplasticPRO_0000001621Add
    BLAST

    Proteomic databases

    PaxDbiQ05753.
    PRIDEiQ05753.

    Expressioni

    Tissue specificityi

    Expressed mainly in chloroplast-containing tissues. Also detected in roots, stems, flower buds, developing siliques and dry seeds.2 Publications

    Developmental stagei

    Highest expression occurs in four-day-old plants and declines as plants develop further.1 Publication

    Inductioni

    By light.1 Publication

    Gene expression databases

    GenevestigatoriQ05753.

    Interactioni

    Subunit structurei

    Interacts with EMB506. No homodimerization observed.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EMB506Q9SQK33EBI-2114020,EBI-2114010

    Protein-protein interaction databases

    BioGridi21979. 1 interaction.
    IntActiQ05753. 1 interaction.
    STRINGi3702.AT5G66055.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05753.
    SMRiQ05753. Positions 211-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati259 – 28830ANK 1Add
    BLAST
    Repeati292 – 32130ANK 2Add
    BLAST
    Repeati325 – 35430ANK 3Add
    BLAST
    Repeati358 – 38730ANK 4Add
    BLAST
    Repeati391 – 42434ANK 5Add
    BLAST

    Sequence similaritiesi

    Contains 5 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0666.
    HOGENOMiHOG000239703.
    InParanoidiQ05753.
    OMAiQIANINR.
    PhylomeDBiQ05753.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view]
    PfamiPF00023. Ank. 2 hits.
    PF12796. Ank_2. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 5 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q05753-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA    50
    GDDFIVGDCL VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI 100
    EPENLVPEEW RDIQAEVNLT KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV 150
    DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD SGASSDGETA VSSPSERVAP 200
    KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK EEKFMLNSRN 250
    PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA 300
    IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA 350
    DINAQDRDGW TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL 400
    YLGREIRTYE VMKLLKEFPL SRHKKRLVTT DEDIE 435

    Note: Found predominantly in leaves and inflorescence stems.

    Length:435
    Mass (Da):48,878
    Last modified:December 13, 2002 - v2
    Checksum:iDE306D487CC894EC
    GO
    Isoform 2 (identifier: Q05753-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         358-358: D → V
         360-435: Missing.

    Note: Found predominantly in flower buds and siliques. Seems to be unable to interact with EMB506.

    Show »
    Length:359
    Mass (Da):40,095
    Checksum:i35DAC3F5B1B911EA
    GO

    Sequence cautioni

    The sequence AAA32812.1 differs from that shown. Reason: Frameshift at position 397.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161R → G in AAA32812. (PubMed:1281700)Curated
    Sequence conflicti30 – 301A → V in AAA32812. (PubMed:1281700)Curated
    Sequence conflicti73 – 731D → E in AAA32812. (PubMed:1281700)Curated
    Sequence conflicti105 – 1051L → P in BAC42148. (PubMed:11910074)Curated
    Sequence conflicti146 – 1461P → Q in BAC42148. (PubMed:11910074)Curated
    Sequence conflicti181 – 1811S → F in AAA32812. (PubMed:1281700)Curated
    Sequence conflicti296 – 2961V → A in AAA32812. (PubMed:1281700)Curated
    Sequence conflicti406 – 4061I → T in AAA32812. (PubMed:1281700)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei358 – 3581D → V in isoform 2. 1 PublicationVSP_030158
    Alternative sequencei360 – 43576Missing in isoform 2. 1 PublicationVSP_030159Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82883 Genomic DNA. Translation: AAA32812.1. Frameshift.
    AB011474 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED98151.1.
    CP002688 Genomic DNA. Translation: AED98152.1.
    AK117484 mRNA. Translation: BAC42148.1.
    AY052363 mRNA. Translation: AAK96554.1.
    BT001055 mRNA. Translation: AAN46809.1.
    PIRiJQ1729.
    RefSeqiNP_569027.2. NM_126003.2. [Q05753-1]
    NP_975000.1. NM_203271.1. [Q05753-2]
    UniGeneiAt.68506.

    Genome annotation databases

    EnsemblPlantsiAT5G66055.1; AT5G66055.1; AT5G66055. [Q05753-1]
    GeneIDi836737.
    KEGGiath:AT5G66055.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82883 Genomic DNA. Translation: AAA32812.1 . Frameshift.
    AB011474 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED98151.1 .
    CP002688 Genomic DNA. Translation: AED98152.1 .
    AK117484 mRNA. Translation: BAC42148.1 .
    AY052363 mRNA. Translation: AAK96554.1 .
    BT001055 mRNA. Translation: AAN46809.1 .
    PIRi JQ1729.
    RefSeqi NP_569027.2. NM_126003.2. [Q05753-1 ]
    NP_975000.1. NM_203271.1. [Q05753-2 ]
    UniGenei At.68506.

    3D structure databases

    ProteinModelPortali Q05753.
    SMRi Q05753. Positions 211-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21979. 1 interaction.
    IntActi Q05753. 1 interaction.
    STRINGi 3702.AT5G66055.1-P.

    Proteomic databases

    PaxDbi Q05753.
    PRIDEi Q05753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G66055.1 ; AT5G66055.1 ; AT5G66055 . [Q05753-1 ]
    GeneIDi 836737.
    KEGGi ath:AT5G66055.

    Organism-specific databases

    GeneFarmi 2586. 230.
    TAIRi AT5G66055.

    Phylogenomic databases

    eggNOGi COG0666.
    HOGENOMi HOG000239703.
    InParanoidi Q05753.
    OMAi QIANINR.
    PhylomeDBi Q05753.

    Enzyme and pathway databases

    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    Genevestigatori Q05753.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view ]
    Pfami PF00023. Ank. 2 hits.
    PF12796. Ank_2. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 5 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis."
      Zhang H., Scheirer D.C., Fowle W.H., Goodman H.M.
      Plant Cell 4:1575-1588(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY LIGHT.
      Strain: cv. Landsberg erecta.
      Tissue: Leaf.
    2. "AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis."
      Garcion C., Guilleminot J., Kroj T., Parcy F., Giraudat J., Devic M.
      Plant J. 48:895-906(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH EMB506, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
      Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    7. "Expression of the Arabidopsis gene Akr coincides with chloroplast development."
      Zhang H., Wang J., Goodman H.M.
      Plant Physiol. 106:1261-1267(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: cv. C24.

    Entry informationi

    Entry nameiAKRP_ARATH
    AccessioniPrimary (citable) accession number: Q05753
    Secondary accession number(s): Q3E852, Q8GYN6, Q940Y0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: December 13, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3