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Q05753 (AKRP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ankyrin repeat domain-containing protein, chloroplastic

Short name=AKRP
Alternative name(s):
Protein EMBRYO DEFECTIVE 2036
Gene names
Name:AKRP
Synonyms:AKR, EMB2036
Ordered Locus Names:At5g66055
ORF Names:K2A18.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the initial differentiation of the proplastid during the embryo development and in plastid differentiation linked to cell differentiation, morphogenesis and organogenesis during the plant life cycle.

Subunit structure

Interacts with EMB506. No homodimerization observed. Ref.2

Subcellular location

Plastidchloroplast Ref.2.

Tissue specificity

Expressed mainly in chloroplast-containing tissues. Also detected in roots, stems, flower buds, developing siliques and dry seeds. Ref.2 Ref.7

Developmental stage

Highest expression occurs in four-day-old plants and declines as plants develop further. Ref.7

Induction

By light. Ref.1

Disruption phenotype

Plants show a developmental arrest of the embryos at the globular stage. Ref.2

Sequence similarities

Contains 5 ANK repeats.

Sequence caution

The sequence AAA32812.1 differs from that shown. Reason: Frameshift at position 397.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainANK repeat
Repeat
Transit peptide
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentchloroplast

Inferred from direct assay Ref.2. Source: TAIR

   Molecular_functionprotein binding

Inferred from physical interaction Ref.2. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EMB506Q9SQK33EBI-2114020,EBI-2114010

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q05753-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Found predominantly in leaves and inflorescence stems.
Isoform 2 (identifier: Q05753-2)

The sequence of this isoform differs from the canonical sequence as follows:
     358-358: D → V
     360-435: Missing.
Note: Found predominantly in flower buds and siliques. Seems to be unable to interact with EMB506.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Chloroplast Potential
Chain37 – 435399Ankyrin repeat domain-containing protein, chloroplastic
PRO_0000001621

Regions

Repeat259 – 28830ANK 1
Repeat292 – 32130ANK 2
Repeat325 – 35430ANK 3
Repeat358 – 38730ANK 4
Repeat391 – 42434ANK 5

Natural variations

Alternative sequence3581D → V in isoform 2.
VSP_030158
Alternative sequence360 – 43576Missing in isoform 2.
VSP_030159

Experimental info

Sequence conflict161R → G in AAA32812. Ref.1
Sequence conflict301A → V in AAA32812. Ref.1
Sequence conflict731D → E in AAA32812. Ref.1
Sequence conflict1051L → P in BAC42148. Ref.5
Sequence conflict1461P → Q in BAC42148. Ref.5
Sequence conflict1811S → F in AAA32812. Ref.1
Sequence conflict2961V → A in AAA32812. Ref.1
Sequence conflict4061I → T in AAA32812. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 13, 2002. Version 2.
Checksum: DE306D487CC894EC

FASTA43548,878
        10         20         30         40         50         60 
MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA GDDFIVGDCL 

        70         80         90        100        110        120 
VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI EPENLVPEEW RDIQAEVNLT 

       130        140        150        160        170        180 
KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD 

       190        200        210        220        230        240 
SGASSDGETA VSSPSERVAP KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK 

       250        260        270        280        290        300 
EEKFMLNSRN PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA 

       310        320        330        340        350        360 
IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA DINAQDRDGW 

       370        380        390        400        410        420 
TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL YLGREIRTYE VMKLLKEFPL 

       430 
SRHKKRLVTT DEDIE 

« Hide

Isoform 2 [UniParc].

Checksum: 35DAC3F5B1B911EA
Show »

FASTA35940,095

References

« Hide 'large scale' references
[1]"Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis."
Zhang H., Scheirer D.C., Fowle W.H., Goodman H.M.
Plant Cell 4:1575-1588(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY LIGHT.
Strain: cv. Landsberg erecta.
Tissue: Leaf.
[2]"AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis."
Garcion C., Guilleminot J., Kroj T., Parcy F., Giraudat J., Devic M.
Plant J. 48:895-906(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH EMB506, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[7]"Expression of the Arabidopsis gene Akr coincides with chloroplast development."
Zhang H., Wang J., Goodman H.M.
Plant Physiol. 106:1261-1267(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: cv. C24.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M82883 Genomic DNA. Translation: AAA32812.1. Frameshift.
AB011474 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED98151.1.
CP002688 Genomic DNA. Translation: AED98152.1.
AK117484 mRNA. Translation: BAC42148.1.
AY052363 mRNA. Translation: AAK96554.1.
BT001055 mRNA. Translation: AAN46809.1.
PIRJQ1729.
RefSeqNP_569027.2. NM_126003.2. [Q05753-1]
NP_975000.1. NM_203271.1. [Q05753-2]
UniGeneAt.68506.

3D structure databases

ProteinModelPortalQ05753.
SMRQ05753. Positions 211-417.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21979. 1 interaction.
IntActQ05753. 1 interaction.
STRING3702.AT5G66055.1-P.

Proteomic databases

PaxDbQ05753.
PRIDEQ05753.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G66055.1; AT5G66055.1; AT5G66055. [Q05753-1]
GeneID836737.
KEGGath:AT5G66055.

Organism-specific databases

GeneFarm2586. 230.
TAIRAT5G66055.

Phylogenomic databases

eggNOGCOG0666.
HOGENOMHOG000239703.
InParanoidQ05753.
OMAQIANINR.
PhylomeDBQ05753.

Gene expression databases

GenevestigatorQ05753.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamPF00023. Ank. 2 hits.
PF12796. Ank_2. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAKRP_ARATH
AccessionPrimary (citable) accession number: Q05753
Secondary accession number(s): Q3E852, Q8GYN6, Q940Y0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 13, 2002
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names