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Q05753

- AKRP_ARATH

UniProt

Q05753 - AKRP_ARATH

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Protein

Ankyrin repeat domain-containing protein, chloroplastic

Gene

AKRP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the initial differentiation of the proplastid during the embryo development and in plastid differentiation linked to cell differentiation, morphogenesis and organogenesis during the plant life cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein, chloroplastic
Short name:
AKRP
Alternative name(s):
Protein EMBRYO DEFECTIVE 2036
Gene namesi
Name:AKRP
Synonyms:AKR, EMB2036
Ordered Locus Names:At5g66055
ORF Names:K2A18.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G66055.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Plants show a developmental arrest of the embryos at the globular stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636ChloroplastSequence AnalysisAdd
BLAST
Chaini37 – 435399Ankyrin repeat domain-containing protein, chloroplasticPRO_0000001621Add
BLAST

Proteomic databases

PaxDbiQ05753.
PRIDEiQ05753.

Expressioni

Tissue specificityi

Expressed mainly in chloroplast-containing tissues. Also detected in roots, stems, flower buds, developing siliques and dry seeds.2 Publications

Developmental stagei

Highest expression occurs in four-day-old plants and declines as plants develop further.1 Publication

Inductioni

By light.1 Publication

Gene expression databases

ExpressionAtlasiQ05753. baseline and differential.
GenevestigatoriQ05753.

Interactioni

Subunit structurei

Interacts with EMB506. No homodimerization observed.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EMB506Q9SQK33EBI-2114020,EBI-2114010

Protein-protein interaction databases

BioGridi21979. 1 interaction.
IntActiQ05753. 1 interaction.
STRINGi3702.AT5G66055.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ05753.
SMRiQ05753. Positions 211-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati259 – 28830ANK 1Add
BLAST
Repeati292 – 32130ANK 2Add
BLAST
Repeati325 – 35430ANK 3Add
BLAST
Repeati358 – 38730ANK 4Add
BLAST
Repeati391 – 42434ANK 5Add
BLAST

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG0666.
HOGENOMiHOG000239703.
InParanoidiQ05753.
OMAiQIANINR.
PhylomeDBiQ05753.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF12796. Ank_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q05753-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA
60 70 80 90 100
GDDFIVGDCL VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI
110 120 130 140 150
EPENLVPEEW RDIQAEVNLT KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV
160 170 180 190 200
DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD SGASSDGETA VSSPSERVAP
210 220 230 240 250
KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK EEKFMLNSRN
260 270 280 290 300
PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA
310 320 330 340 350
IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA
360 370 380 390 400
DINAQDRDGW TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL
410 420 430
YLGREIRTYE VMKLLKEFPL SRHKKRLVTT DEDIE

Note: Found predominantly in leaves and inflorescence stems.

Length:435
Mass (Da):48,878
Last modified:December 13, 2002 - v2
Checksum:iDE306D487CC894EC
GO
Isoform 2 (identifier: Q05753-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     358-358: D → V
     360-435: Missing.

Note: Found predominantly in flower buds and siliques. Seems to be unable to interact with EMB506.

Show »
Length:359
Mass (Da):40,095
Checksum:i35DAC3F5B1B911EA
GO

Sequence cautioni

The sequence AAA32812.1 differs from that shown. Reason: Frameshift at position 397. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161R → G in AAA32812. (PubMed:1281700)Curated
Sequence conflicti30 – 301A → V in AAA32812. (PubMed:1281700)Curated
Sequence conflicti73 – 731D → E in AAA32812. (PubMed:1281700)Curated
Sequence conflicti105 – 1051L → P in BAC42148. (PubMed:11910074)Curated
Sequence conflicti146 – 1461P → Q in BAC42148. (PubMed:11910074)Curated
Sequence conflicti181 – 1811S → F in AAA32812. (PubMed:1281700)Curated
Sequence conflicti296 – 2961V → A in AAA32812. (PubMed:1281700)Curated
Sequence conflicti406 – 4061I → T in AAA32812. (PubMed:1281700)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei358 – 3581D → V in isoform 2. 1 PublicationVSP_030158
Alternative sequencei360 – 43576Missing in isoform 2. 1 PublicationVSP_030159Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82883 Genomic DNA. Translation: AAA32812.1. Frameshift.
AB011474 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED98151.1.
CP002688 Genomic DNA. Translation: AED98152.1.
AK117484 mRNA. Translation: BAC42148.1.
AY052363 mRNA. Translation: AAK96554.1.
BT001055 mRNA. Translation: AAN46809.1.
PIRiJQ1729.
RefSeqiNP_569027.2. NM_126003.2. [Q05753-1]
NP_975000.1. NM_203271.1. [Q05753-2]
UniGeneiAt.68506.

Genome annotation databases

EnsemblPlantsiAT5G66055.1; AT5G66055.1; AT5G66055. [Q05753-1]
GeneIDi836737.
KEGGiath:AT5G66055.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82883 Genomic DNA. Translation: AAA32812.1 . Frameshift.
AB011474 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED98151.1 .
CP002688 Genomic DNA. Translation: AED98152.1 .
AK117484 mRNA. Translation: BAC42148.1 .
AY052363 mRNA. Translation: AAK96554.1 .
BT001055 mRNA. Translation: AAN46809.1 .
PIRi JQ1729.
RefSeqi NP_569027.2. NM_126003.2. [Q05753-1 ]
NP_975000.1. NM_203271.1. [Q05753-2 ]
UniGenei At.68506.

3D structure databases

ProteinModelPortali Q05753.
SMRi Q05753. Positions 211-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21979. 1 interaction.
IntActi Q05753. 1 interaction.
STRINGi 3702.AT5G66055.1-P.

Proteomic databases

PaxDbi Q05753.
PRIDEi Q05753.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G66055.1 ; AT5G66055.1 ; AT5G66055 . [Q05753-1 ]
GeneIDi 836737.
KEGGi ath:AT5G66055.

Organism-specific databases

GeneFarmi 2586. 230.
TAIRi AT5G66055.

Phylogenomic databases

eggNOGi COG0666.
HOGENOMi HOG000239703.
InParanoidi Q05753.
OMAi QIANINR.
PhylomeDBi Q05753.

Gene expression databases

ExpressionAtlasi Q05753. baseline and differential.
Genevestigatori Q05753.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view ]
Pfami PF00023. Ank. 2 hits.
PF12796. Ank_2. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 5 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis."
    Zhang H., Scheirer D.C., Fowle W.H., Goodman H.M.
    Plant Cell 4:1575-1588(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY LIGHT.
    Strain: cv. Landsberg erecta.
    Tissue: Leaf.
  2. "AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis."
    Garcion C., Guilleminot J., Kroj T., Parcy F., Giraudat J., Devic M.
    Plant J. 48:895-906(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH EMB506, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  7. "Expression of the Arabidopsis gene Akr coincides with chloroplast development."
    Zhang H., Wang J., Goodman H.M.
    Plant Physiol. 106:1261-1267(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: cv. C24.

Entry informationi

Entry nameiAKRP_ARATH
AccessioniPrimary (citable) accession number: Q05753
Secondary accession number(s): Q3E852, Q8GYN6, Q940Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 13, 2002
Last modified: November 26, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3