Ankyrin repeat domain-containing protein, chloroplastic precursor

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Q05753 (AKRP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ankyrin repeat domain-containing protein, chloroplastic
Short name=AKRP

Alternative name(s):
Protein EMBRYO DEFECTIVE 2036

Gene names

Name:	AKRP
Synonyms:	AKR, EMB2036
Ordered Locus Names:	At5g66055
ORF Names:	K2A18.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the initial differentiation of the proplastid during the embryo development and in plastid differentiation linked to cell differentiation, morphogenesis and organogenesis during the plant life cycle.
Subunit structure	Interacts with EMB506. No homodimerization observed. Ref.2
Subcellular location	Plastid › chloroplast Ref.2.
Tissue specificity	Expressed mainly in chloroplast-containing tissues. Also detected in roots, stems, flower buds, developing siliques and dry seeds. Ref.2 Ref.7
Developmental stage	Highest expression occurs in four-day-old plants and declines as plants develop further. Ref.7
Induction	By light. Ref.1
Disruption phenotype	Plants show a developmental arrest of the embryos at the globular stage. Ref.2
Sequence similarities	Contains 5 ANK repeats.
Sequence caution	The sequence AAA32812.1 differs from that shown. Reason: Frameshift at position 397.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat Transit peptide
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	chloroplast Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EMB506	Q9SQK3	3	EBI-2114020,EBI-2114010

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q05753-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Found predominantly in leaves and inflorescence stems.

Isoform 2 (identifier: Q05753-2) The sequence of this isoform differs from the canonical sequence as follows: 358-358: D → V 360-435: Missing.
Note: Found predominantly in flower buds and siliques. Seems to be unable to interact with EMB506.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 36

Chloroplast Potential

Chain

37 – 435

399

Ankyrin repeat domain-containing protein, chloroplastic

PRO_0000001621

Regions

Repeat

259 – 288

ANK 1

Repeat

292 – 321

ANK 2

Repeat

325 – 354

ANK 3

Repeat

358 – 387

ANK 4

Repeat

391 – 424

ANK 5

Natural variations

Alternative sequence

358

D → V in isoform 2.

VSP_030158

Alternative sequence

360 – 435

Missing in isoform 2.

VSP_030159

Experimental info

Sequence conflict

R → G in AAA32812. Ref.1

Sequence conflict

A → V in AAA32812. Ref.1

Sequence conflict

D → E in AAA32812. Ref.1

Sequence conflict

105

L → P in BAC42148. Ref.5

Sequence conflict

146

P → Q in BAC42148. Ref.5

Sequence conflict

181

S → F in AAA32812. Ref.1

Sequence conflict

296

V → A in AAA32812. Ref.1

Sequence conflict

406

I → T in AAA32812. Ref.1

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified December 13, 2002. Version 2. Checksum: DE306D487CC894EC Show »	FASTA	435	48,878
10 20 30 40 50 60 MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA GDDFIVGDCL 70 80 90 100 110 120 VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI EPENLVPEEW RDIQAEVNLT 130 140 150 160 170 180 KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD 190 200 210 220 230 240 SGASSDGETA VSSPSERVAP KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK 250 260 270 280 290 300 EEKFMLNSRN PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA 310 320 330 340 350 360 IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA DINAQDRDGW 370 380 390 400 410 420 TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL YLGREIRTYE VMKLLKEFPL 430 SRHKKRLVTT DEDIE « Hide
Isoform 2 [UniParc]. Checksum: 35DAC3F5B1B911EA Show »	FASTA	359	40,095
10 20 30 40 50 60 MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA GDDFIVGDCL 70 80 90 100 110 120 VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI EPENLVPEEW RDIQAEVNLT 130 140 150 160 170 180 KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD 190 200 210 220 230 240 SGASSDGETA VSSPSERVAP KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK 250 260 270 280 290 300 EEKFMLNSRN PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA 310 320 330 340 350 IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA DINAQDRVG « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis." Zhang H., Scheirer D.C., Fowle W.H., Goodman H.M. Plant Cell 4:1575-1588(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY LIGHT. Strain: cv. Landsberg erecta. Tissue: Leaf.
[2]	"AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis." Garcion C., Guilleminot J., Kroj T., Parcy F., Giraudat J., Devic M. Plant J. 48:895-906(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH EMB506, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[7]	"Expression of the Arabidopsis gene Akr coincides with chloroplast development." Zhang H., Wang J., Goodman H.M. Plant Physiol. 106:1261-1267(1994) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. C24.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M82883 Genomic DNA. Translation: AAA32812.1. Frameshift. AB011474 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED98151.1. CP002688 Genomic DNA. Translation: AED98152.1. AK117484 mRNA. Translation: BAC42148.1. AY052363 mRNA. Translation: AAK96554.1. BT001055 mRNA. Translation: AAN46809.1.
IPI	IPI00523216. IPI00545683.
PIR	JQ1729.
RefSeq	NP_569027.2. NM_126003.2. NP_975000.1. NM_203271.1.
UniGene	At.68506.
3D structure databases
ProteinModelPortal	Q05753.
SMR	Q05753. Positions 211-417.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q05753. 1 interaction.
STRING	3702.AT5G66055.1-P.
Proteomic databases
PaxDb	Q05753.
PRIDE	Q05753.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G66055.1; AT5G66055.1; AT5G66055.
GeneID	836737.
KEGG	ath:AT5G66055.
Organism-specific databases
GeneFarm	2586. 230.
TAIR	At5g66055.
Phylogenomic databases
eggNOG	COG0666.
HOGENOM	HOG000239703.
InParanoid	Q05753.
OMA	KIAQEME.
PhylomeDB	Q05753.
ProtClustDB	CLSN2680622.
Gene expression databases
ArrayExpress	Q05753.
Genevestigator	Q05753.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Pfam	PF00023. Ank. 2 hits. PF12796. Ank_2. 1 hit. [Graphical view]
SMART	SM00248. ANK. 5 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKRP_ARATH

Accession

Primary (citable) accession number: Q05753
Secondary accession number(s): Q3E852, Q8GYN6, Q940Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 13, 2002
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Recent format changes

Overview of recent format changes

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents