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Protein

Ankyrin repeat domain-containing protein, chloroplastic

Gene

AKRP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the initial differentiation of the proplastid during the embryo development and in plastid differentiation linked to cell differentiation, morphogenesis and organogenesis during the plant life cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein, chloroplastic
Short name:
AKRP
Alternative name(s):
Protein EMBRYO DEFECTIVE 2036
Gene namesi
Name:AKRP
Synonyms:AKR, EMB2036
Ordered Locus Names:At5g66055
ORF Names:K2A18.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G66055.

Subcellular locationi

  • Plastidchloroplast 1 Publication

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Plants show a developmental arrest of the embryos at the globular stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 36ChloroplastSequence analysisAdd BLAST36
ChainiPRO_000000162137 – 435Ankyrin repeat domain-containing protein, chloroplasticAdd BLAST399

Proteomic databases

PaxDbiQ05753.

PTM databases

iPTMnetiQ05753.

Expressioni

Tissue specificityi

Expressed mainly in chloroplast-containing tissues. Also detected in roots, stems, flower buds, developing siliques and dry seeds.2 Publications

Developmental stagei

Highest expression occurs in four-day-old plants and declines as plants develop further.1 Publication

Inductioni

By light.1 Publication

Gene expression databases

ExpressionAtlasiQ05753. baseline and differential.
GenevisibleiQ05753. AT.

Interactioni

Subunit structurei

Interacts with EMB506. No homodimerization observed.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EMB506Q9SQK33EBI-2114020,EBI-2114010

Protein-protein interaction databases

BioGridi21979. 1 interactor.
IntActiQ05753. 1 interactor.
STRINGi3702.AT5G66055.1.

Structurei

3D structure databases

ProteinModelPortaliQ05753.
SMRiQ05753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati259 – 288ANK 1Add BLAST30
Repeati292 – 321ANK 2Add BLAST30
Repeati325 – 354ANK 3Add BLAST30
Repeati358 – 387ANK 4Add BLAST30
Repeati391 – 424ANK 5Add BLAST34

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000239703.
InParanoidiQ05753.
OMAiCLRLGHH.
OrthoDBiEOG09360CBT.
PhylomeDBiQ05753.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q05753-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA
60 70 80 90 100
GDDFIVGDCL VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI
110 120 130 140 150
EPENLVPEEW RDIQAEVNLT KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV
160 170 180 190 200
DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD SGASSDGETA VSSPSERVAP
210 220 230 240 250
KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK EEKFMLNSRN
260 270 280 290 300
PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA
310 320 330 340 350
IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA
360 370 380 390 400
DINAQDRDGW TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL
410 420 430
YLGREIRTYE VMKLLKEFPL SRHKKRLVTT DEDIE
Note: Found predominantly in leaves and inflorescence stems.
Length:435
Mass (Da):48,878
Last modified:December 13, 2002 - v2
Checksum:iDE306D487CC894EC
GO
Isoform 2 (identifier: Q05753-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     358-358: D → V
     360-435: Missing.

Note: Found predominantly in flower buds and siliques. Seems to be unable to interact with EMB506.
Show »
Length:359
Mass (Da):40,095
Checksum:i35DAC3F5B1B911EA
GO

Sequence cautioni

The sequence AAA32812 differs from that shown. Reason: Frameshift at position 397.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16R → G in AAA32812 (PubMed:1281700).Curated1
Sequence conflicti30A → V in AAA32812 (PubMed:1281700).Curated1
Sequence conflicti73D → E in AAA32812 (PubMed:1281700).Curated1
Sequence conflicti105L → P in BAC42148 (PubMed:11910074).Curated1
Sequence conflicti146P → Q in BAC42148 (PubMed:11910074).Curated1
Sequence conflicti181S → F in AAA32812 (PubMed:1281700).Curated1
Sequence conflicti296V → A in AAA32812 (PubMed:1281700).Curated1
Sequence conflicti406I → T in AAA32812 (PubMed:1281700).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030158358D → V in isoform 2. 1 Publication1
Alternative sequenceiVSP_030159360 – 435Missing in isoform 2. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82883 Genomic DNA. Translation: AAA32812.1. Frameshift.
AB011474 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED98151.1.
CP002688 Genomic DNA. Translation: AED98152.1.
AK117484 mRNA. Translation: BAC42148.1.
AY052363 mRNA. Translation: AAK96554.1.
BT001055 mRNA. Translation: AAN46809.1.
PIRiJQ1729.
RefSeqiNP_569027.2. NM_126003.3. [Q05753-1]
NP_975000.1. NM_203271.2. [Q05753-2]
UniGeneiAt.68506.

Genome annotation databases

EnsemblPlantsiAT5G66055.1; AT5G66055.1; AT5G66055. [Q05753-1]
GeneIDi836737.
GrameneiAT5G66055.1; AT5G66055.1; AT5G66055.
KEGGiath:AT5G66055.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82883 Genomic DNA. Translation: AAA32812.1. Frameshift.
AB011474 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED98151.1.
CP002688 Genomic DNA. Translation: AED98152.1.
AK117484 mRNA. Translation: BAC42148.1.
AY052363 mRNA. Translation: AAK96554.1.
BT001055 mRNA. Translation: AAN46809.1.
PIRiJQ1729.
RefSeqiNP_569027.2. NM_126003.3. [Q05753-1]
NP_975000.1. NM_203271.2. [Q05753-2]
UniGeneiAt.68506.

3D structure databases

ProteinModelPortaliQ05753.
SMRiQ05753.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21979. 1 interactor.
IntActiQ05753. 1 interactor.
STRINGi3702.AT5G66055.1.

PTM databases

iPTMnetiQ05753.

Proteomic databases

PaxDbiQ05753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G66055.1; AT5G66055.1; AT5G66055. [Q05753-1]
GeneIDi836737.
GrameneiAT5G66055.1; AT5G66055.1; AT5G66055.
KEGGiath:AT5G66055.

Organism-specific databases

TAIRiAT5G66055.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000239703.
InParanoidiQ05753.
OMAiCLRLGHH.
OrthoDBiEOG09360CBT.
PhylomeDBiQ05753.

Miscellaneous databases

PROiQ05753.

Gene expression databases

ExpressionAtlasiQ05753. baseline and differential.
GenevisibleiQ05753. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKRP_ARATH
AccessioniPrimary (citable) accession number: Q05753
Secondary accession number(s): Q3E852, Q8GYN6, Q940Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 13, 2002
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.