Reviewed, UniProtKB/Swiss-Prot Q05753 (AKRP_ARATH)

Names and origin

Protein names

Recommended name:
    Ankyrin repeat domain-containing protein, chloroplastic
      Short name=AKRP
Alternative name(s):
    Protein EMBRYO DEFECTIVE 2036

Gene names

Name:	AKRP
Synonyms:	AKR, EMB2036
Ordered Locus Names:	At5g66055
ORF Names:	K2A18.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the initial differentiation of the proplastid during the embryo development and in plastid differentiation linked to cell differentiation, morphogenesis and organogenesis during the plant life cycle.
Subunit structure	Interacts with EMB506. No homodimerization observed. Ref.2
Subcellular location	Plastid › chloroplast Ref.2.
Tissue specificity	Expressed mainly in chloroplast-containing tissues. Also detected in roots, stems, flower buds, developing siliques and dry seeds. Ref.2 Ref.6
Developmental stage	Highest expression occurs in four-day-old plants and declines as plants develop further. Ref.6
Induction	By light. Ref.1
Disruption phenotype	Plants show a developmental arrest of the embryos at the globular stage. Ref.2
Sequence similarities	Contains 5 ANK repeats.
Sequence caution	The sequence AAA32812.1 differs from that shown. Reason: Frameshift at position 397.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat Transit peptide
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	chloroplast Ref.2 Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.2 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EMB506	Q9SQK3	2	EBI-2114020,EBI-2114010

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q05753-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Found predominantly in leaves and inflorescence stems.

Isoform 2 (identifier: Q05753-2)

The sequence of this isoform differs from the canonical sequence as follows:
     358-358: D → V
     360-435: Missing.

Note: Found predominantly in flower buds and siliques. Seems to be unable to interact with EMB506.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Chloroplast Potential
	Chain	37 – 435	399	Ankyrin repeat domain-containing protein, chloroplastic		PRO_0000001621
Regions
	Repeat	259 – 288	30	ANK 1
	Repeat	292 – 321	30	ANK 2
	Repeat	325 – 354	30	ANK 3
	Repeat	358 – 387	30	ANK 4
	Repeat	391 – 424	34	ANK 5
Natural variations
	Alternative sequence	358	1	D → V in isoform 2.		VSP_030158
	Alternative sequence	360 – 435	76	Missing in isoform 2.		VSP_030159
Experimental info
	Sequence conflict	16	1	R → G in AAA32812. Ref.1
	Sequence conflict	30	1	A → V in AAA32812. Ref.1
	Sequence conflict	73	1	D → E in AAA32812. Ref.1
	Sequence conflict	105	1	L → P in BAC42148. Ref.4
	Sequence conflict	146	1	P → Q in BAC42148. Ref.4
	Sequence conflict	181	1	S → F in AAA32812. Ref.1
	Sequence conflict	296	1	V → A in AAA32812. Ref.1
	Sequence conflict	406	1	I → T in AAA32812. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 13, 2002. Version 2. Checksum: DE306D487CC894EC	FASTA	435	48,878
	10 20 30 40 50 60 MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA GDDFIVGDCL 70 80 90 100 110 120 VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI EPENLVPEEW RDIQAEVNLT 130 140 150 160 170 180 KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD 190 200 210 220 230 240 SGASSDGETA VSSPSERVAP KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK 250 260 270 280 290 300 EEKFMLNSRN PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA 310 320 330 340 350 360 IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA DINAQDRDGW 370 380 390 400 410 420 TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL YLGREIRTYE VMKLLKEFPL 430 SRHKKRLVTT DEDIE
	Isoform 2. Checksum: 35DAC3F5B1B911EA	FASTA	359	40,095
	10 20 30 40 50 60 MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA GDDFIVGDCL 70 80 90 100 110 120 VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI EPENLVPEEW RDIQAEVNLT 130 140 150 160 170 180 KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD 190 200 210 220 230 240 SGASSDGETA VSSPSERVAP KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK 250 260 270 280 290 300 EEKFMLNSRN PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA 310 320 330 340 350 IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA DINAQDRVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis." Zhang H., Scheirer D.C., Fowle W.H., Goodman H.M. Plant Cell 4:1575-1588(1992) [PubMed: 1281700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY LIGHT. Strain: cv. Landsberg erecta. Tissue: Leaf.
[2]	"AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis." Garcion C., Guilleminot J., Kroj T., Parcy F., Giraudat J., Devic M. Plant J. 48:895-906(2006) [PubMed: 17092312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH EMB506, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed: 9679202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Expression of the Arabidopsis gene Akr coincides with chloroplast development." Zhang H., Wang J., Goodman H.M. Plant Physiol. 106:1261-1267(1994) [PubMed: 12232406] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. C24.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M82883 Genomic DNA. Translation: AAA32812.1. Frameshift. AB011474 Genomic DNA. No translation available. AK117484 mRNA. Translation: BAC42148.1. AY052363 mRNA. Translation: AAK96554.1. BT001055 mRNA. Translation: AAN46809.1.
IPI	IPI00523216. IPI00545683.
PIR	JQ1729.
RefSeq	NP_569027.2. NP_975000.1.
UniGene	At.68506
3D structure databases
SMR	Q05753. Positions 239-419.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q05753. 1 interaction.
Genome annotation databases
GeneID	836737.
GenomeReviews	Gene locus AT5G66055 in contig BA000015_GR.
KEGG	ath:AT5G66055.
NMPDR	fig|3702.1.peg.28700.
Organism-specific databases
GeneFarm	2586. 230.
TAIR	At5g66055.
Phylogenomic databases
eggNOG	KOG0504.
HOGENOM	HBG317301.
InParanoid	Q05753.
OMA	LLRNSAN.
PhylomeDB	Q05753.
Gene expression databases
ArrayExpress	Q05753.
Genevestigator	Q05753.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 2 hits. [Graphical view]
SMART	SM00248. ANK. 5 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKRP_ARATH

Accession

Primary (citable) accession number: Q05753
Secondary accession number(s): Q3E852, Q8GYN6, Q940Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 13, 2002
Last modified:	February 9, 2010
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)