Q05744 (CATD_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cathepsin D
EC=3.4.23.5

Cleaved into the following 2 chains:

1. Cathepsin D light chain
2. Cathepsin D heavy chain

Gene names

Name:

CTSD

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acid protease active in intracellular protein breakdown. In chicken it is a key enzyme for yolk formation as it is capable of catalyzing intra oocytic break down of protein components of both vitellogenin and VLDL.
Catalytic activity	Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.
Subunit structure	Consists of a light chain and a heavy chain.
Subcellular location	Lysosome.
Tissue specificity	Oocytic yolk, preovulatory follicles, liver.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 63	43	Activation peptide Potential		PRO_0000025966
	Chain	64 – 398	335	Cathepsin D		PRO_0000025967
	Chain	64 – 157	94	Cathepsin D light chain Probable		PRO_0000025968
	Chain	158 – 398	241	Cathepsin D heavy chain Probable		PRO_0000025969
Sites
	Active site	96	1	By similarity
	Active site	283	1	By similarity
Amino acid modifications
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 116		By similarity
	Disulfide bond	274 ↔ 278		By similarity
	Disulfide bond	317 ↔ 354		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05744 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 584C99E2755AA2B1	FASTA	398	43,298
	10 20 30 40 50 60 MAPRGLLVLL LLALVGPCAA LIRIPLTKFT STRRMLTEVG SEIPDMNAIT QFLKFKLGFA 70 80 90 100 110 120 DLAEPTPEIL KNYMDAQYYG EIGIGTPPQK FTVVFDTGSS NLWVPSVHCH LLDIACLLHH 130 140 150 160 170 180 KYDASKSSTY VENGTEFAIH YGTGSLSGFL SQDTVTLGNL KIKNQIFGEA VKQPGITFIA 190 200 210 220 230 240 AKFDGILGMA FPRISVDKVT PFFDNVMQQK LIEKNIFSFY LNRDPTAQPG GELLLGGTDP 250 260 270 280 290 300 KYYSGDFSWV NVTRKAYWQV HMDSVDVANG LTLCKGGCEA IVDTGTSLIT GPTKEVKELQ 310 320 330 340 350 360 TAIGAKPLIK GQYVISCDKI SSLPVVTLML GGKPYQLTGE QYVFKVSAQG ETICLSGFSG 370 380 390 LDVPPPGGPL WILGDVFIGP YYTVFDRDND SVGFAKCV

References

[1]	"Molecular cloning and functional characterization of chicken cathepsin D, a key enzyme for yolk formation." Retzek H., Steyrer E., Sanders E.J., Nimpf J., Schneider W.J. DNA Cell Biol. 11:661-672(1992) [PubMed: 1418623] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 64-97. Tissue: Follicular cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S49650 mRNA. Translation: AAB24157.1.
IPI	IPI00600003.
PIR	I51185.
RefSeq	NP_990508.1. NM_205177.1.
UniGene	Gga.1094.
3D structure databases
ProteinModelPortal	Q05744.
SMR	Q05744. Positions 65-396.
ModBase	Search...
Protein-protein interaction databases
STRING	Q05744.
Protein family/group databases
MEROPS	A01.009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000010676; ENSGALP00000010662; ENSGALG00000006613.
GeneID	396090.
KEGG	gga:396090.
Organism-specific databases
CTD	1509.
Phylogenomic databases
eggNOG	veNOG11338.
GeneTree	ENSGT00600000084042.
HOGENOM	HBG590923.
HOVERGEN	HBG000482.
InParanoid	Q05744.
OMA	SGFMGID.
OrthoDB	EOG40GCR5.
PhylomeDB	Q05744.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
KO	K01379.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATD_CHICK

Accession

Primary (citable) accession number: Q05744

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program